How to understand its output
The first window below the input area is a graphical representation
of all the peptides that can be obtained upon translation in
all the ORF. All the 6 frames are shown one beneath the other and
are numbered on the left side from 0 to 5. Along each frame, peptides
that can be obtained (given the presence of start/stop codon pairs)
are displayed as colored bars. A yellow grid every 10 bp is superimposed,
and red lines are drawn every 100 bases. Labels are placed in the bottom
part of this graph, for navigational purposes. Would the input
DNA sequence exceed 600 bp, the graph can be scrolled acting on the scrollbar
placed above the graph. By clicking on anyone of the colored bars, more
details are shown in the segments below:
A textual area below the colored graph contains further information
on the specified translation object. It consists of one or more lines,
depending on the length of the transcript, which begins with the frame index
(from 0 to 5), the aminoacid sequence in three-letter notation,
and finally the location of begin and end on the DNA sequence.
The last window which is present in this application is a colored and 10base
formatted image of the DNA sequence. It is colored using the same color
as the currently selected peptide sequence. It is thus very
simple to see the entire information set.
References
Doelz,R.(1990)BioCompanion, Biocomputing Essentials series, ISBN 3-905 434-00-8
Author:Luca I.G. TOLDO,
"Edition date: 28 February 1997