Physical Mapping Calculator - Library Construction
This very simple calculator shows how the library redundancy and %% of genome covered depends on the number of clones in a library. Redundancy (or the number of genome equivalents contained in a library) grows linearly with the number of clones and is limited by ten in this and Random Fingerprinting calculator. A proportion of genome covered (or a probability that a given sequence is found in a library) is known as Clarke and Carbon (1976) formula: 1-exp(-redundancy). Thanks to Jared Roach for suggesting me to include this calculator in the set and the original reference to Robbins (1944).
Approximate sizes of human chromosomes are given below (frankly, I don't remember where I've got this table from so if anything is wrong please let me know).
Clarke, L. and Carbon, J. (1976) A colony bank containing synthetic ColE1 hybrid plasmids representative of the entire E. coli genome. Cell, 9, 91-101.
Robbins, H. E. (1944) On the measure of a random set. Ann. Math. Stat., 15, 70-74.
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Copyright 1996 Andrei Grigoriev
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