AnnHyb's help - Multi-alignment cleaner
This function allow you to edit multi-alignment of sequences done with CLUSTALW (ALN as file extention).
You can select a sequence as a reference for multi-alignment or calculate a consensus sequence with variable homology percent
1) Open the CLUSTALW multi-alignment in AnnHYb
2) Select the multi-alignment do you want to clean
3) You have two possibility for editing your CLUSTALW output:
Select a reference sequence
Check on the "Select a reference sequence" radio button
Choose the sequence do you want to use as a reference for the multi-alignment in the list box
Make a consensus sequence
Check the "Make a consensus sequence" radio button
Enter the homology percent for the consensus
4) Choose a length for the output line in bases (default is 60 b like in default CLUSTALW)
5) Click the
Clear multi-alignment
button
The result is shown in the "
Results" window
Example of consensus sequence (85% homologie) from a CLUSTALW multi-alignment: