AnnHyb's help - Multi-alignment cleaner

This function allow you to edit multi-alignment of sequences done with CLUSTALW (ALN as file extention).

You can select a sequence as a reference for multi-alignment or calculate a consensus sequence with variable homology percent

1) Open the CLUSTALW multi-alignment in AnnHYb

2) Select the multi-alignment do you want to clean

3) You have two possibility for editing your CLUSTALW output:

• Select a reference sequence


Check on the "Select a reference sequence" radio button
Choose the sequence do you want to use as a reference for the multi-alignment in the list box





• Make a consensus sequence


Check the "Make a consensus sequence" radio button
Enter the homology percent for the consensus

4) Choose a length for the output line in bases (default is 60 b like in default CLUSTALW)

5) Click the Clear multi-alignment button

The result is shown in the "Results" window



Example of consensus sequence (85% homologie) from a CLUSTALW multi-alignment: