osprai/trunk/io_module.py

"""
io_module
---------

Input/Output module for converting files to Biosensor Array class.
Supported sensorgram types include Biosensor, CLAMP, SPRit, and Plexera ICM.
Supported microarray types include GAL and ISB Map.

.. moduleauthor:: Christopher Lausted, 
                  Institute for Systems Biology, 
                  OSPRAI developers.

Examples::

  >>> import io_module as io
  >>> ba1 = io.readsprit("spritdata.txt")
  >>> ba1 = io.readclamp("spritdata.txt")
  >>> ba1 = io.readicmtxt("icmdata.txt")
  >>> ba1 = io.readbiosensor("icmdata.txt")
  >>> ba1 = io.readcsv("rawdata.csv")
  >>> ba2 = io.applygal(ba1, "galfile.gal")
  >>> ba2 = io.applykey(ba1, "keyfile.tsv")
  >>> ba3 = io.applymethod(ba2, "icmmethod.xls")
  >>> io.writesprit(ba3, "newspritfile.txt")
  >>> io.writeclamp(ba3, "newclampfile.txt")
"""
__version__ = "110214"


## Import libraries.
from copy import deepcopy
from os import getcwd
from time import mktime
import numpy as np                  ## Numpy array library.
import ba_class as ba               ## Our Biosensor Array class.
reload(ba)

def readsprit(fname):
    """
    Read a SPRit text file into a ba class.
    It has two tab-delimited columns and two header lines.
    Here is a very simple example with 2 rois and 3 datapoints::
    
      Elapsed Time (Seconds)   Average Intensity (Pixel Intensity)
      BEGIN
      0.000000e+000            2.863145e+003
      5.013000e+000            2.863367e+003
      1.002500e+001            2.862950e+003
      0.000000e+000            2.862875e+003
      5.013000e+000            2.862510e+003
    """
    
    ## Try to open file. Return tiny ba object if it fails.
    ba0 = ba.BiosensorArray(1,1)
    try:
        fp = open(fname, "r")
    except IOError:
        print 'Error: Cannot open file %s for reading' % fname
        return ba0
    
    ## Check header lines for signature text.
    txt = fp.readline()  # 'Elapsed Time...'
    txt = fp.readline()  # 'BEGIN'
    if ('BEGIN' not in txt):
        print "Error: Second line is not BEGIN."
        fp.close()
        return ba0
    
    ## Put data into one big text string and close.
    txtfile = fp.readlines()
    fp.close()
    
    ## Change text into array
    sprtable = np.zeros((len(txtfile),2), dtype=float)  ## Redimension Nx2
    for i in range(len(txtfile)):
        txt = txtfile[i].split('\t')
        sprtable[i,0] = float(txt[0])  ## Time column.
        sprtable[i,1] = float(txt[1])  ## Signal column.
    
    ## Find number of ROIs in file.
    for i in range(1,len(sprtable)):
        ## See when the time drops back to zero.
        if (sprtable[i,0] < sprtable[i-1,0]): break
    rows = i  ## Datapoints or new array rows.
    rois = int(len(sprtable) / rows)
    
    ## Move data the old-fashioned iterative way.
    ba0 = ba.BiosensorArray(rois,rows)
    k = 0
    for i in range(rois):
        for j in range(rows):
            ba0.roi[i].time[j] = sprtable[k,0]
            ba0.roi[i].value[j] = sprtable[k,1]
            k +=1
            
    return ba0
    """End of readsprit() function"""
    
    
def writesprit(ba0, fname):
    """
    Write a ba class to a SPRit text file.
    """
    fp=file(fname,'w')
    fp.write("Elapsed Time (Seconds)\tAverage Intensity (Pixel Intensity)\r\n")
    fp.write("BEGIN\r\n")
    for roi in range(len(ba0.roi)):                   ## Could also use ba.rois.
        for dpoint in range(len(ba0.roi[0].time)):    ## Could also use ba.dpoints.
            txt = "%f\t%f\r\n" % (ba0.roi[roi].time[dpoint], ba0.roi[roi].value[dpoint])
            fp.write(txt)
    fp.close
    print "File %s saved in %s." % (fname, getcwd())
    return
    """End of writesprit() function"""
    
    
def readclamp(fname):
    """
    Read a Clamp text file into a ba class.
    It has two tab-delimited columns per SPR flowcell/roi..
    It has a varying number of header lines with injection information.
    Here is a very simple example with 2 rois and 3 datapoints::
    
      Vers 3.41 Data
      Conc1   0       0       0       0
      Start1  301.5   301.5   301.5   301.5
      Stop1   949.8   949.8   949.8   949.8
      RInd1   0       0       0       0
      Conc2   0       0       0       0
      Start2  986.4   0       0       0
      Stop2   1626    0       0       0
      RInd2   0       0       0       0
      Flow    1       1       1       1
      Time1   Data1   Time2   Data2
      0.094   0.062   0.094   0.053
      1.094   0.026   1.094   0.05
      2.094   0.119   2.094   0.055
    """
    
    print "This feature is under construction."
    return
    """"End of readclamp() function"""
    
    
def writeclamp(ba0, fname):
    """
    Write a ba class to a Clamp text file.
    """
    
    fp=file(fname,'w')
    ## First header line.
    fp.write("Vers 3.41 Data\r\n")
    
    ## Next write injection information.
    for inj in range(len(ba0.roi[0].injconc)):
        fp.write("Conc%i" % (inj+1))
        for roi in ba0.roi: fp.write("\t%.3f" % roi.injconc[inj])
        fp.write("\r\nStart%i" % (inj+1))
        for roi in ba0.roi: fp.write("\t%.3f" % roi.injstart[inj])
        fp.write("\r\nStop%i" % (inj+1))
        for roi in ba0.roi: fp.write("\t%.3f" % roi.injstop[inj])
        fp.write("\r\nRInd%i" % (inj+1))
        for roi in ba0.roi: fp.write("\t%.3f" % roi.injrind[inj])
    ## Next write flowrate line.
    fp.write("\r\nFlow")
    for roi in ba0.roi: fp.write("\t%.3f" % roi.flow)
    fp.write("\r\n")
    
    ## Write sensorgram data header line.
    for i,roi in enumerate(ba0.roi):
        if (i>0): fp.write("\t")
        fp.write("Time%i" % (i+1))
        fp.write("\t")
        fp.write(roi.name)
    fp.write("\r\n")
    ## Write sensorgram data lines. Three decimal places.
    for dpoint in range(len(ba0.roi[0].time)):
        for i,roi in enumerate(ba0.roi):
            if (i>0): fp.write("\t")
            fp.write("%.3f\t%.3f" % (roi.time[dpoint], roi.value[dpoint]))
        fp.write("\r\n")
        
    ## Close file handle and print message.
    fp.close
    print "File %s saved in %s." % (fname, getcwd())
    return
    """End of writeclamp() function"""
    
    
def readicmtxt(fname):
    """
    Read a ICM text file into a ba class.
    Here is a very simple example of the tab-delimited format::
    
      03/05/2010 13:37:21.312   249.408   0.000   0.000
      03/05/2010 13:37:22.312   249.306   0.000   0.000
    """ 

    ## Try to open file. Return tiny ba object if it fails.
    ba0 = ba.BiosensorArray(1,1)
    try:
        fp = open(fname, "r")
    except IOError:
        print 'Error: Cannot open file %s for reading' % fname
        return ba0
    
    ## Put data into one big text string and close.
    txtfile = fp.readlines()
    fp.close()
    dpoints = len(txtfile)
    rois = txtfile[0].count("\t")  ## Usually 25.
    
    ## Create and size ba object.
    print "This ICM file has %i datapoints for %i ROIs." % (dpoints, rois)
    ba0 = ba.BiosensorArray(rois, dpoints)
    
    ## Determine experiment start time so we can make other times relative.
    x = txtfile[0].split("\t")  ## E.g. "03/05/2010 13:37:20.218"
    t0 = __icmtime2sec(x[0])      ## E.g. 1267825040.22
    
    ## Move the data from txtfile to ba0.
    for i, txtline in enumerate(txtfile):
        x = txtline.split("\t")
        tx = __icmtime2sec(x[0]) - t0
        for j in range(1,rois):
            ba0.roi[j].time[i] = tx
            ba0.roi[j].value[i] = float(x[j])
            
    return ba0
    """End of readicmtxt"""


def __icmtime2sec(timetxt):
    """
    Take a time of the form "03/05/2010 15:19:27.312" and return seconds.
    """
    cal, clock = timetxt.split(" ")
    mm, dd, yy = cal.split("/")
    hh, min, ss = clock.split(":")
    stime = (int(yy), int(mm), int(dd), int(hh), int(min), 0, 0, 0, 0)
    return mktime(stime) + float(ss)
    """End of __icmtime2sec"""
    
    
def readbiosensor(fname):
    """
    Read a Biacore-style text file into a ba class.
    Here is a very simple example of the tab-delimited format::
    
      Ab1 Fc=4- 1_X  Ab1 Fc=4 -1_Y  Ab2 Fc=4 -1_X  Ab2 Fc=4 -1_Y
      13.1           23.7644        93.1           0.713912
      13.6           23.4265        93.6           0.0541172
      14.1           23.1625        94.1           0.332768
      14.6           23.5752        94.6           0.849459
    """ 

    ## Try to open file. Return tiny ba object if it fails.
    ba0 = ba.BiosensorArray(1,1)
    try:
        fp = open(fname, "r")
    except IOError:
        print 'Error: Cannot open file %s for reading' % fname
        return ba0
    
    ## Read header line.  Check number of pairs of _X and _Y labels.
    txthdr = fp.readline()
    cols = txthdr.count("Fc=")
    xys = txthdr.count("_X\t") + txthdr.count("_Y\t")+1
    if ((cols != xys) or ((cols%2) != 0)):
        print "Error: This is not a valid Biosensor file."
        return ba0
        
    ## Put data into one big text string and close.
    txtfile = fp.readlines()
    fp.close()
    dpoints = len(txtfile)
    rois = int(cols/2)
    
    ## Create and size ba object.
    print "This Biosensor file has %i datapoints for %i ROIs." % (dpoints, rois)
    ba0 = ba.BiosensorArray(rois, dpoints)
    
    ## Get names of ROIs from header like "Ab1 Fc=4 - 1_Y"
    txthdr = txthdr.replace(" -","-").replace("- ","-") ## Remove unwanted spaces.
    names = txthdr.split("\t")                          ## Use tab delimiter.
    for j in range(rois):
        name = names[j*2+1].replace("_Y","").strip(" -")
        name = name.partition("Fc=")          ## Now a 3-tuple.
        ba0.roi[j].name = name[0]             ## Text left of Fc.
        ba0.roi[j].desc = ("Fc=" + name[2])   ## Text right of Fc.
        
    ## Move the data from txtfile to ba0.
    for i, txtline in enumerate(txtfile):
        x = txtline.split("\t")
        for j in range(rois):
            ba0.roi[j].time[i] = float(x[j*2+0])
            ba0.roi[j].value[i] = float(x[j*2+1])
    
    return ba0
    """End of readbiosensor()"""


def writebiosensor(ba0, fname):
    """
    Write a ba class to a Biosensor text file.
    """
    
    ## This is like a simplified Clamp file.
    fp=file(fname,'w')
    ## Write sensorgram data header line.
    for i,roi in enumerate(ba0.roi):
        if (i>0): fp.write("\t")
        name = roi.name.strip() + " " + roi.desc.strip()
        fp.write("%s_X\t%s_Y" % (name, name))
    ## Write sensorgram data lines. Three decimal places.
    for dpoint in range(len(ba0.roi[0].time)):
        fp.write("\r\n")
        for i,roi in enumerate(ba0.roi):
            if (i>0): fp.write("\t")
            fp.write("%.3f\t%.3f" % (roi.time[dpoint], roi.value[dpoint]))
    
    ## Close file handle and print message.
    fp.close
    print "File %s saved in %s." % (fname, getcwd())
    return
    """End of writebiosensor()"""


def readcsv(fname):
    """
    Read a comma-separated value text file into a ba class.
    The first column contains time data while the others contain response data.
    Here is a very simple example of the format::
    
      1.0001, 23.7644, 0.7139
      2.0001, 23.4265, 0.0541
      3.0001, 23.1625, 0.3327
      4.0001, 23.5752, 0.8494
    """ 

    ## Try to open file. Return tiny ba object if it fails.
    ba0 = ba.BiosensorArray(1,1)
    try:
        fp = open(fname, "r")
    except IOError:
        print 'Error: Cannot open file %s for reading' % fname
        return ba0
      
    ## Put data into one big text string, determine array size, and close.
    txtfile = fp.readlines()  ## One big text string.
    fp.close()
    dpoints = len(txtfile)  ## Rows in the csv file.
    rois = len(txtfile[0].split(',')) - 1  ## Columns are comma-delimited.
    
    ## Move the data from txtfile to ba0.
    ba0 = ba.BiosensorArray(rois, dpoints)
    for i, txtline in enumerate(txtfile):
        x = txtline.split(',')
        for j in range(rois):
            ba0.roi[j].time[i] = float(x[0])
            ba0.roi[j].value[i] = float(x[j+1])
    
    print "The file %s has %i ROIs and %i datapoints." % (fname, rois, dpoints)
    return ba0
    """End of readcsv()"""


def applygal(ba0, fname):
    """
    Read a Gal file and apply its microarray information.
    
    *(This function is under construction)*
    """
    print "This feature is under construction."
    return
    """End of applygal()"""
    
    
def applykey(ba0, fname):
    """
    Read a Key file and apply its microarray information.
    Multiple background ROIs are not yet supported.
    Here is a very simple example::
    
      No. Description1    Description2    Background ROI  Col     Row
      1   Rat TNF         Antibody01      2               1       1
      2   ratIgG          Antibody02      4               2       1
      3   Hum TNF         Antibody03      4               3       1
      4   humIgG          Antibody04      2               4       1
    """
    
    ## Try to open file. Return unchanged ba object if it fails.
    try:
        fp = open(fname, "r")
    except IOError:
        print 'Cannot open file %s for reading' % fname
        return ba0
    ## Check header lines for signature text.
    txt = fp.readline()
    if ("No." not in txt):
        print "Error: The header line is unfamiliar."
        fp.close()
        return ba0
    
    ## Read all text, convert to 2d list, then six lists (one for each column).
    ## There's probably an easier way to do this. Maybe in CSV module.
    txtfile = fp.readlines()
    fp.close()
    ## Change text into array
    keytable = txtfile[:]  ## Dimension rows in new 2d list.
    for i in range(len(txtfile)):
        txt = str(txtfile[i].strip())
        keytable[i] = txt.split('\t')
    ## Use list comprehensions to get one list for each column.
    id = [int(x[0]) for x in keytable]    ## First column is integer.
    desc1 = [x[1] for x in keytable]      ## Second column is text.
    desc2 = [x[2] for x in keytable]      ## Third column is text.
    bg = [int(x[3]) for x in keytable]    ## Fourth column is integer. Change base1 to base0?
    col = [int(x[4]) for x in keytable]   ## Fifth column is integer.
    row = [int(x[5]) for x in keytable]   ## Sixth column is integer.
    
    ## Check if key file looks valid.  These tests are not very thorough!
    keys = 1 + max(id) - min(id)
    if (ba0.rois != keys):
        print "Error: We have %i ROIs but %i keyfile entries." % (ba0.rois, keys)
        return ba0
    if ((min(bg) < min(id)) or (max(bg) > max (id))):
        print "Error: at least one specified background ROI does not exist"
        print "Identifiers (id) range from %i to %i." % (min(id), max(id))
        print "Background references range from %i to %i." % (min(bg), max(bg))
        return ba0
        
    ## Create new object and put data in it.
    ba1 = deepcopy(ba0)
    for i in range(len(id)):
        ba1.roi[id[i]-1].name = desc1[i] 
        ba1.roi[id[i]-1].desc = desc2[i]
        ba1.roi[id[i]-1].bgroi = [bg[i]]  ## ToDo: Decide if bg will be id or index. Base1 now.
        ba1.roi[id[i]-1].spotx = col[i]
        ba1.roi[id[i]-1].spoty = row[i]
    
    print "Successfully loaded information for %i ROIs." % keys
    return ba1
    """End of applykey()"""
    
    
def applymethod(ba0, fname):
    """
    Read a ICM Analyte/Method xls file and apply its information.
    
    *(This feature is under construction.)*
    """
    print "This feature is under construction."
    return
    """End of applymethod()"""
    
    
def outputbindinglevels(ba0, fname, interval, *baselines):
    """
    Measure the binding level changes at multiple intervals along a sensorgram.
    Write the data to a file.  If interval is 500 and baselines are [100, 1100] then
    binding levels are changes between 100-600s and between 1100-01600s.
    Average 30s of data.
    """
    fp=file(fname,'w')
    
    ## Measurements
    for iroi in ba0.roi:
        fp.write("%i\t%s" % (iroi.index, iroi.name))
        for j in baselines:
            t1 = j
            t2 = j + interval
            y1 = np.average(iroi.time2val(t1-15, t1+15))
            y2 = np.average(iroi.time2val(t2-15, t2+15))
            fp.write("\t%.3f" % (y2-y1))
        fp.write("\n")
    fp.close
    print "File %s saved in %s." % (fname, getcwd())
    return
    """End of outputbindinglevels()"""


## Here are a few lines to test this module.
if __name__ == '__main__':
    print "This module isn't a stand-alone app."

################################# End of module #################################
Revision:	54
Committed:	Wed Feb 16 21:29:02 2011 UTC (8 years, 7 months ago) by clausted
File size:	16560 byte(s)
Log Message:	Added fit_module and mdl_module to sphinx documentation.
Line	User	Rev	File contents
1	clausted	7	"""
2	clausted	47	io_module
3			---------
4	clausted	7
5	clausted	47	Input/Output module for converting files to Biosensor Array class.
6			Supported sensorgram types include Biosensor, CLAMP, SPRit, and Plexera ICM.
7			Supported microarray types include GAL and ISB Map.
8
9			.. moduleauthor:: Christopher Lausted,
10			Institute for Systems Biology,
11	clausted	54	OSPRAI developers.
12
13	clausted	47	Examples::
14
15			>>> import io_module as io
16			>>> ba1 = io.readsprit("spritdata.txt")
17			>>> ba1 = io.readclamp("spritdata.txt")
18			>>> ba1 = io.readicmtxt("icmdata.txt")
19			>>> ba1 = io.readbiosensor("icmdata.txt")
20	clausted	49	>>> ba1 = io.readcsv("rawdata.csv")
21	clausted	47	>>> ba2 = io.applygal(ba1, "galfile.gal")
22			>>> ba2 = io.applykey(ba1, "keyfile.tsv")
23			>>> ba3 = io.applymethod(ba2, "icmmethod.xls")
24			>>> io.writesprit(ba3, "newspritfile.txt")
25			>>> io.writeclamp(ba3, "newclampfile.txt")
26	clausted	7	"""
27	clausted	54	__version__ = "110214"
28	clausted	7
29	clausted	47
30	clausted	7	## Import libraries.
31			from copy import deepcopy
32			from os import getcwd
33			from time import mktime
34			import numpy as np ## Numpy array library.
35			import ba_class as ba ## Our Biosensor Array class.
36			reload(ba)
37
38			def readsprit(fname):
39			"""
40			Read a SPRit text file into a ba class.
41			It has two tab-delimited columns and two header lines.
42	clausted	47	Here is a very simple example with 2 rois and 3 datapoints::
43	clausted	7
44	clausted	47	Elapsed Time (Seconds) Average Intensity (Pixel Intensity)
45			BEGIN
46			0.000000e+000 2.863145e+003
47			5.013000e+000 2.863367e+003
48			1.002500e+001 2.862950e+003
49			0.000000e+000 2.862875e+003
50			5.013000e+000 2.862510e+003
51	clausted	7	"""
52
53			## Try to open file. Return tiny ba object if it fails.
54			ba0 = ba.BiosensorArray(1,1)
55			try:
56			fp = open(fname, "r")
57			except IOError:
58			print 'Error: Cannot open file %s for reading' % fname
59			return ba0
60
61			## Check header lines for signature text.
62			txt = fp.readline() # 'Elapsed Time...'
63			txt = fp.readline() # 'BEGIN'
64			if ('BEGIN' not in txt):
65			print "Error: Second line is not BEGIN."
66			fp.close()
67			return ba0
68
69			## Put data into one big text string and close.
70			txtfile = fp.readlines()
71			fp.close()
72
73			## Change text into array
74			sprtable = np.zeros((len(txtfile),2), dtype=float) ## Redimension Nx2
75			for i in range(len(txtfile)):
76			txt = txtfile[i].split('\t')
77			sprtable[i,0] = float(txt[0]) ## Time column.
78			sprtable[i,1] = float(txt[1]) ## Signal column.
79
80			## Find number of ROIs in file.
81			for i in range(1,len(sprtable)):
82			## See when the time drops back to zero.
83			if (sprtable[i,0] < sprtable[i-1,0]): break
84			rows = i ## Datapoints or new array rows.
85			rois = int(len(sprtable) / rows)
86
87			## Move data the old-fashioned iterative way.
88			ba0 = ba.BiosensorArray(rois,rows)
89			k = 0
90			for i in range(rois):
91			for j in range(rows):
92			ba0.roi[i].time[j] = sprtable[k,0]
93			ba0.roi[i].value[j] = sprtable[k,1]
94			k +=1
95
96			return ba0
97	clausted	47	"""End of readsprit() function"""
98	clausted	7
99
100			def writesprit(ba0, fname):
101	clausted	47	"""
102			Write a ba class to a SPRit text file.
103			"""
104	clausted	7	fp=file(fname,'w')
105			fp.write("Elapsed Time (Seconds)\tAverage Intensity (Pixel Intensity)\r\n")
106			fp.write("BEGIN\r\n")
107			for roi in range(len(ba0.roi)): ## Could also use ba.rois.
108			for dpoint in range(len(ba0.roi[0].time)): ## Could also use ba.dpoints.
109			txt = "%f\t%f\r\n" % (ba0.roi[roi].time[dpoint], ba0.roi[roi].value[dpoint])
110			fp.write(txt)
111			fp.close
112			print "File %s saved in %s." % (fname, getcwd())
113	clausted	47	return
114			"""End of writesprit() function"""
115	clausted	7
116
117			def readclamp(fname):
118			"""
119			Read a Clamp text file into a ba class.
120			It has two tab-delimited columns per SPR flowcell/roi..
121			It has a varying number of header lines with injection information.
122	clausted	47	Here is a very simple example with 2 rois and 3 datapoints::
123	clausted	7
124	clausted	47	Vers 3.41 Data
125			Conc1 0 0 0 0
126			Start1 301.5 301.5 301.5 301.5
127			Stop1 949.8 949.8 949.8 949.8
128			RInd1 0 0 0 0
129			Conc2 0 0 0 0
130			Start2 986.4 0 0 0
131			Stop2 1626 0 0 0
132			RInd2 0 0 0 0
133			Flow 1 1 1 1
134			Time1 Data1 Time2 Data2
135			0.094 0.062 0.094 0.053
136			1.094 0.026 1.094 0.05
137			2.094 0.119 2.094 0.055
138	clausted	7	"""
139
140			print "This feature is under construction."
141	clausted	47	return
142			""""End of readclamp() function"""
143	clausted	7
144
145			def writeclamp(ba0, fname):
146	clausted	47	"""
147			Write a ba class to a Clamp text file.
148			"""
149	clausted	7
150			fp=file(fname,'w')
151			## First header line.
152			fp.write("Vers 3.41 Data\r\n")
153
154			## Next write injection information.
155			for inj in range(len(ba0.roi[0].injconc)):
156			fp.write("Conc%i" % (inj+1))
157			for roi in ba0.roi: fp.write("\t%.3f" % roi.injconc[inj])
158			fp.write("\r\nStart%i" % (inj+1))
159			for roi in ba0.roi: fp.write("\t%.3f" % roi.injstart[inj])
160			fp.write("\r\nStop%i" % (inj+1))
161			for roi in ba0.roi: fp.write("\t%.3f" % roi.injstop[inj])
162			fp.write("\r\nRInd%i" % (inj+1))
163			for roi in ba0.roi: fp.write("\t%.3f" % roi.injrind[inj])
164			## Next write flowrate line.
165			fp.write("\r\nFlow")
166			for roi in ba0.roi: fp.write("\t%.3f" % roi.flow)
167			fp.write("\r\n")
168
169			## Write sensorgram data header line.
170			for i,roi in enumerate(ba0.roi):
171			if (i>0): fp.write("\t")
172			fp.write("Time%i" % (i+1))
173			fp.write("\t")
174			fp.write(roi.name)
175			fp.write("\r\n")
176			## Write sensorgram data lines. Three decimal places.
177			for dpoint in range(len(ba0.roi[0].time)):
178			for i,roi in enumerate(ba0.roi):
179			if (i>0): fp.write("\t")
180			fp.write("%.3f\t%.3f" % (roi.time[dpoint], roi.value[dpoint]))
181			fp.write("\r\n")
182
183			## Close file handle and print message.
184			fp.close
185			print "File %s saved in %s." % (fname, getcwd())
186	clausted	47	return
187			"""End of writeclamp() function"""
188	clausted	7
189
190			def readicmtxt(fname):
191			"""
192			Read a ICM text file into a ba class.
193	clausted	47	Here is a very simple example of the tab-delimited format::
194	clausted	7
195	clausted	47	03/05/2010 13:37:21.312 249.408 0.000 0.000
196			03/05/2010 13:37:22.312 249.306 0.000 0.000
197	clausted	7	"""
198
199			## Try to open file. Return tiny ba object if it fails.
200			ba0 = ba.BiosensorArray(1,1)
201			try:
202			fp = open(fname, "r")
203			except IOError:
204			print 'Error: Cannot open file %s for reading' % fname
205			return ba0
206
207			## Put data into one big text string and close.
208			txtfile = fp.readlines()
209			fp.close()
210			dpoints = len(txtfile)
211			rois = txtfile[0].count("\t") ## Usually 25.
212
213			## Create and size ba object.
214			print "This ICM file has %i datapoints for %i ROIs." % (dpoints, rois)
215			ba0 = ba.BiosensorArray(rois, dpoints)
216
217			## Determine experiment start time so we can make other times relative.
218			x = txtfile[0].split("\t") ## E.g. "03/05/2010 13:37:20.218"
219	clausted	50	t0 = __icmtime2sec(x[0]) ## E.g. 1267825040.22
220	clausted	7
221			## Move the data from txtfile to ba0.
222			for i, txtline in enumerate(txtfile):
223			x = txtline.split("\t")
224	clausted	50	tx = __icmtime2sec(x[0]) - t0
225	clausted	7	for j in range(1,rois):
226			ba0.roi[j].time[i] = tx
227			ba0.roi[j].value[i] = float(x[j])
228
229			return ba0
230	clausted	47	"""End of readicmtxt"""
231	clausted	7
232
233	clausted	50	def __icmtime2sec(timetxt):
234	clausted	7	"""
235			Take a time of the form "03/05/2010 15:19:27.312" and return seconds.
236			"""
237			cal, clock = timetxt.split(" ")
238			mm, dd, yy = cal.split("/")
239			hh, min, ss = clock.split(":")
240			stime = (int(yy), int(mm), int(dd), int(hh), int(min), 0, 0, 0, 0)
241			return mktime(stime) + float(ss)
242	clausted	50	"""End of __icmtime2sec"""
243	clausted	7
244
245	clausted	9	def readbiosensor(fname):
246			"""
247			Read a Biacore-style text file into a ba class.
248	clausted	47	Here is a very simple example of the tab-delimited format::
249	clausted	9
250	clausted	47	Ab1 Fc=4- 1_X Ab1 Fc=4 -1_Y Ab2 Fc=4 -1_X Ab2 Fc=4 -1_Y
251			13.1 23.7644 93.1 0.713912
252			13.6 23.4265 93.6 0.0541172
253			14.1 23.1625 94.1 0.332768
254			14.6 23.5752 94.6 0.849459
255	clausted	9	"""
256
257			## Try to open file. Return tiny ba object if it fails.
258			ba0 = ba.BiosensorArray(1,1)
259			try:
260			fp = open(fname, "r")
261			except IOError:
262			print 'Error: Cannot open file %s for reading' % fname
263			return ba0
264
265			## Read header line. Check number of pairs of _X and _Y labels.
266			txthdr = fp.readline()
267			cols = txthdr.count("Fc=")
268			xys = txthdr.count("_X\t") + txthdr.count("_Y\t")+1
269			if ((cols != xys) or ((cols%2) != 0)):
270			print "Error: This is not a valid Biosensor file."
271			return ba0
272
273			## Put data into one big text string and close.
274			txtfile = fp.readlines()
275			fp.close()
276			dpoints = len(txtfile)
277			rois = int(cols/2)
278
279			## Create and size ba object.
280			print "This Biosensor file has %i datapoints for %i ROIs." % (dpoints, rois)
281			ba0 = ba.BiosensorArray(rois, dpoints)
282
283			## Get names of ROIs from header like "Ab1 Fc=4 - 1_Y"
284			txthdr = txthdr.replace(" -","-").replace("- ","-") ## Remove unwanted spaces.
285			names = txthdr.split("\t") ## Use tab delimiter.
286			for j in range(rois):
287			name = names[j*2+1].replace("_Y","").strip(" -")
288			name = name.partition("Fc=") ## Now a 3-tuple.
289			ba0.roi[j].name = name[0] ## Text left of Fc.
290			ba0.roi[j].desc = ("Fc=" + name[2]) ## Text right of Fc.
291
292			## Move the data from txtfile to ba0.
293			for i, txtline in enumerate(txtfile):
294			x = txtline.split("\t")
295			for j in range(rois):
296			ba0.roi[j].time[i] = float(x[j*2+0])
297			ba0.roi[j].value[i] = float(x[j*2+1])
298
299			return ba0
300	clausted	47	"""End of readbiosensor()"""
301	clausted	9
302
303			def writebiosensor(ba0, fname):
304	clausted	47	"""
305			Write a ba class to a Biosensor text file.
306			"""
307	clausted	9
308			## This is like a simplified Clamp file.
309			fp=file(fname,'w')
310			## Write sensorgram data header line.
311			for i,roi in enumerate(ba0.roi):
312			if (i>0): fp.write("\t")
313			name = roi.name.strip() + " " + roi.desc.strip()
314			fp.write("%s_X\t%s_Y" % (name, name))
315			## Write sensorgram data lines. Three decimal places.
316			for dpoint in range(len(ba0.roi[0].time)):
317			fp.write("\r\n")
318			for i,roi in enumerate(ba0.roi):
319			if (i>0): fp.write("\t")
320			fp.write("%.3f\t%.3f" % (roi.time[dpoint], roi.value[dpoint]))
321
322			## Close file handle and print message.
323			fp.close
324			print "File %s saved in %s." % (fname, getcwd())
325	clausted	47	return
326			"""End of writebiosensor()"""
327	clausted	9
328
329	clausted	49	def readcsv(fname):
330			"""
331			Read a comma-separated value text file into a ba class.
332			The first column contains time data while the others contain response data.
333	clausted	51	Here is a very simple example of the format::
334	clausted	49
335			1.0001, 23.7644, 0.7139
336			2.0001, 23.4265, 0.0541
337			3.0001, 23.1625, 0.3327
338			4.0001, 23.5752, 0.8494
339			"""
340
341			## Try to open file. Return tiny ba object if it fails.
342			ba0 = ba.BiosensorArray(1,1)
343			try:
344			fp = open(fname, "r")
345			except IOError:
346			print 'Error: Cannot open file %s for reading' % fname
347			return ba0
348
349			## Put data into one big text string, determine array size, and close.
350			txtfile = fp.readlines() ## One big text string.
351			fp.close()
352			dpoints = len(txtfile) ## Rows in the csv file.
353			rois = len(txtfile[0].split(',')) - 1 ## Columns are comma-delimited.
354
355			## Move the data from txtfile to ba0.
356			ba0 = ba.BiosensorArray(rois, dpoints)
357			for i, txtline in enumerate(txtfile):
358			x = txtline.split(',')
359			for j in range(rois):
360			ba0.roi[j].time[i] = float(x[0])
361			ba0.roi[j].value[i] = float(x[j+1])
362
363			print "The file %s has %i ROIs and %i datapoints." % (fname, rois, dpoints)
364			return ba0
365			"""End of readcsv()"""
366
367
368	clausted	7	def applygal(ba0, fname):
369	clausted	47	"""
370			Read a Gal file and apply its microarray information.
371
372			(This function is under construction)
373			"""
374	clausted	7	print "This feature is under construction."
375	clausted	47	return
376			"""End of applygal()"""
377	clausted	7
378
379			def applykey(ba0, fname):
380			"""
381			Read a Key file and apply its microarray information.
382			Multiple background ROIs are not yet supported.
383	clausted	47	Here is a very simple example::
384	clausted	7
385	clausted	47	No. Description1 Description2 Background ROI Col Row
386			1 Rat TNF Antibody01 2 1 1
387			2 ratIgG Antibody02 4 2 1
388			3 Hum TNF Antibody03 4 3 1
389			4 humIgG Antibody04 2 4 1
390	clausted	7	"""
391
392			## Try to open file. Return unchanged ba object if it fails.
393			try:
394			fp = open(fname, "r")
395			except IOError:
396			print 'Cannot open file %s for reading' % fname
397			return ba0
398			## Check header lines for signature text.
399			txt = fp.readline()
400			if ("No." not in txt):
401			print "Error: The header line is unfamiliar."
402			fp.close()
403			return ba0
404
405			## Read all text, convert to 2d list, then six lists (one for each column).
406			## There's probably an easier way to do this. Maybe in CSV module.
407			txtfile = fp.readlines()
408			fp.close()
409			## Change text into array
410			keytable = txtfile[:] ## Dimension rows in new 2d list.
411			for i in range(len(txtfile)):
412			txt = str(txtfile[i].strip())
413			keytable[i] = txt.split('\t')
414			## Use list comprehensions to get one list for each column.
415	clausted	14	id = [int(x[0]) for x in keytable] ## First column is integer.
416			desc1 = [x[1] for x in keytable] ## Second column is text.
417			desc2 = [x[2] for x in keytable] ## Third column is text.
418			bg = [int(x[3]) for x in keytable] ## Fourth column is integer. Change base1 to base0?
419	clausted	7	col = [int(x[4]) for x in keytable] ## Fifth column is integer.
420			row = [int(x[5]) for x in keytable] ## Sixth column is integer.
421
422			## Check if key file looks valid. These tests are not very thorough!
423			keys = 1 + max(id) - min(id)
424			if (ba0.rois != keys):
425			print "Error: We have %i ROIs but %i keyfile entries." % (ba0.rois, keys)
426			return ba0
427			if ((min(bg) < min(id)) or (max(bg) > max (id))):
428	clausted	14	print "Error: at least one specified background ROI does not exist"
429			print "Identifiers (id) range from %i to %i." % (min(id), max(id))
430			print "Background references range from %i to %i." % (min(bg), max(bg))
431	clausted	7	return ba0
432
433			## Create new object and put data in it.
434			ba1 = deepcopy(ba0)
435			for i in range(len(id)):
436			ba1.roi[id[i]-1].name = desc1[i]
437			ba1.roi[id[i]-1].desc = desc2[i]
438	clausted	14	ba1.roi[id[i]-1].bgroi = [bg[i]] ## ToDo: Decide if bg will be id or index. Base1 now.
439	clausted	7	ba1.roi[id[i]-1].spotx = col[i]
440			ba1.roi[id[i]-1].spoty = row[i]
441
442			print "Successfully loaded information for %i ROIs." % keys
443			return ba1
444	clausted	47	"""End of applykey()"""
445	clausted	7
446
447			def applymethod(ba0, fname):
448	clausted	47	"""
449			Read a ICM Analyte/Method xls file and apply its information.
450
451			(This feature is under construction.)
452			"""
453	clausted	7	print "This feature is under construction."
454	clausted	47	return
455			"""End of applymethod()"""
456	clausted	14
457
458	clausted	7
459	clausted	14	def outputbindinglevels(ba0, fname, interval, *baselines):
460			"""
461			Measure the binding level changes at multiple intervals along a sensorgram.
462			Write the data to a file. If interval is 500 and baselines are [100, 1100] then
463			binding levels are changes between 100-600s and between 1100-01600s.
464			Average 30s of data.
465			"""
466			fp=file(fname,'w')
467
468			## Measurements
469			for iroi in ba0.roi:
470			fp.write("%i\t%s" % (iroi.index, iroi.name))
471			for j in baselines:
472			t1 = j
473			t2 = j + interval
474			y1 = np.average(iroi.time2val(t1-15, t1+15))
475			y2 = np.average(iroi.time2val(t2-15, t2+15))
476			fp.write("\t%.3f" % (y2-y1))
477			fp.write("\n")
478			fp.close
479			print "File %s saved in %s." % (fname, getcwd())
480	clausted	47	return
481			"""End of outputbindinglevels()"""
482	clausted	7
483	clausted	14
484	clausted	7	## Here are a few lines to test this module.
485			if __name__ == '__main__':
486			print "This module isn't a stand-alone app."
487
488	clausted	47	################################# End of module #################################