Ebbie v3.0.8
Ebbie: automated analysis and storage of small RNA cloning data using a dynamic web
server.
Motivation
Sequencing projects, such as cloning of small RNAs, result in thousands
of sequencing files. Here we describe an automated
analysis pipeline, which excises the cloned RNA; uses BlastN
to search it against various databases and allows standardized
annotation using cgi-scripts. After annotating the new clone,
the sequence and its annotation are stored in a MySQL database as well
as in a flat file for subsequent BlastN searches. This
sequence analysis pipeline eliminates manual mistakes, allows the
discovery of multiply cloned sequences and reliably stores sequences
and their annotations.
Availability
Ebbie's permanent home will be bioinformatics.org/ebbie
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