Ebbie v3.0.8

Ebbie: automated analysis and storage of small RNA cloning data using a dynamic web server.

Motivation

Sequencing projects, such as cloning of small RNAs, result in thousands of sequencing files. Here we describe an automated analysis pipeline, which excises the cloned RNA; uses BlastN to search it against various databases and allows standardized annotation using cgi-scripts. After annotating the new clone, the sequence and its annotation are stored in a MySQL database as well as in a flat file for subsequent BlastN searches. This sequence analysis pipeline eliminates manual mistakes, allows the discovery of multiply cloned sequences and reliably stores sequences and their annotations.

Availability

Ebbie's permanent home will be bioinformatics.org/ebbie
Please select a database here. Once selected, Ebbie will work with the same database unless the database selection is changed here.