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    Opportunity: Research Assistant (#76668) @ Lawrence Berkeley National Laboratory -- Berkeley, CA (US)
    Submitted by Jeff Todd; posted on Saturday, December 07, 2013



    Under the general direction and supervision of the principal investigator (PI) and the direct oversight beamline scientists, the Research Assistant will assist with the SIBYLS and HHMI wet-laboratory space that includes ensuring all equipment is operational and well maintained, provide user support for mail-in services and aid beamline scientists in their respective research.


    Laboratory and General Assistance:
    • Receive, catalog, and prepare samples from collaborators and users for structural characterization.
    • Assist in the data entry on UNIX, OSX and Windows based computers.
    • Assist in the day-to-day preparation of buffered solutions and media for beamline personnel.
    • Perform assigned general laboratory duties as requested by the scientists and PI.
    • Place purchase orders with vendors.
    • Responsible for daily maintenance of work area.
    • Assist with maintaining equipment including Mill-Q purification system, PCR, AKTA Explorer and Ettan, BioRad HPLC and refrigerators and freezers.
    • Maintain laboratory compliance with safety regulations.
    • Maintain inventories of essential laboratory consumables.
    Research Support:
    • Assist in performing expression of proteins in bacteria, including maintenance, growth and harvest of bacterial cultures for special projects.
    • Practical experience in optimizing cell-free protein synthesis.
    • Assist in performing separation and preliminary analysis of proteins using chromatographic techniques.
    • Use standard molecular biological techniques to subclone genes for recombinant expression.
    • Assist with duties using biochemical laboratory equipment, including BioRad HPLC system, BioRad C1000 quantitative PCR cycler, centrifuges, UV-VIS spectrophotometers, pH meters, balances, etc.
    • Maintain an accurate and detailed laboratory notebook of all work performed. Ensure that others can duplicate results.


    • Experience and course work within the last 4 years in cell and molecular biology.
    • Demonstrated experience with common biochemical laboratory equipment, including BioRad HPLC system, prep- and ultra-centrifuges, UV-VIS spectrophotometers, pH meters, etc.
    • Demonstrated experience in liquid chromatographic techniques.
    • Knowledge of basic molecular biology techniques.
    • Demonstrated ability to organize, analyze and document research activities and maintain laboratory experimental records.
    • Familiarity with basic computer spreadsheet software to perform data analysis.
    • Experience using personal computers using programs such as MS Word, Excel, PowerPoint, etc.
    • Experience with various cell free protein synthesis systems.
    • Experience with long-transcript RNA transcription and purification.
    • Good interpersonal, communication, and organizational skills.
    • Ability to work as a team member to accomplish research goals.
    • Willingness to work flexible hours.
    • Ability to multi-task efficiently.
    • Ability to perform routine responsibilities independently, work dependably and flexibly.


    • Bachelor's degree in biochemistry, molecular biology, chemistry, or related field.


    This is an indefinite (career) appointment.


    Berkeley, CA


    For full consideration, follow the link to the job posting and apply directly to Berkeley Lab. As part of the online application process, please complete a profile, including uploading attachments relevant to the opportunity. Be sure to reference where you found the position (Bioinformatics.Org).[...]76668


    Berkeley Lab is an affirmative action/equal opportunity employer committed to the development of a diverse workforce.

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