BACKGROUND

RESPONSIBILITIES

• Receive, catalog, and prepare samples from collaborators and users for structural characterization.
• Assist in the data entry on UNIX, OSX and Windows based computers.
• Assist in the day-to-day preparation of buffered solutions and media for beamline personnel.
• Perform assigned general laboratory duties as requested by the scientists and PI.
• Place purchase orders with vendors.
• Responsible for daily maintenance of work area.
• Assist with maintaining equipment including Mill-Q purification system, PCR, AKTA Explorer and Ettan, BioRad HPLC and refrigerators and freezers.
• Maintain laboratory compliance with safety regulations.
• Maintain inventories of essential laboratory consumables.

• Assist in performing expression of proteins in bacteria, including maintenance, growth and harvest of bacterial cultures for special projects.
• Practical experience in optimizing cell-free protein synthesis.
• Assist in performing separation and preliminary analysis of proteins using chromatographic techniques.
• Use standard molecular biological techniques to subclone genes for recombinant expression.
• Assist with duties using biochemical laboratory equipment, including BioRad HPLC system, BioRad C1000 quantitative PCR cycler, centrifuges, UV-VIS spectrophotometers, pH meters, balances, etc.
• Maintain an accurate and detailed laboratory notebook of all work performed. Ensure that others can duplicate results.

REQUIREMENTS

• Experience and course work within the last 4 years in cell and molecular biology.
• Demonstrated experience with common biochemical laboratory equipment, including BioRad HPLC system, prep- and ultra-centrifuges, UV-VIS spectrophotometers, pH meters, etc.
• Demonstrated experience in liquid chromatographic techniques.
• Knowledge of basic molecular biology techniques.
• Demonstrated ability to organize, analyze and document research activities and maintain laboratory experimental records.
• Familiarity with basic computer spreadsheet software to perform data analysis.
• Experience using personal computers using programs such as MS Word, Excel, PowerPoint, etc.
• Experience with various cell free protein synthesis systems.
• Experience with long-transcript RNA transcription and purification.
• Good interpersonal, communication, and organizational skills.
• Ability to work as a team member to accomplish research goals.
• Willingness to work flexible hours.
• Ability to multi-task efficiently.
• Ability to perform routine responsibilities independently, work dependably and flexibly.

PREFERENCES

• Bachelor's degree in biochemistry, molecular biology, chemistry, or related field.

TERMS

LOCALE

HOW TO APPLY

POLICY