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    Opportunity: Research Scientist I (#909) @ Broad Institute -- Cambridge, MA (US)
    Submitted by Laura Bonfitto; posted on Sunday, November 11, 2012


    This is an exciting opportunity working under the direction of Broad Institute Fellow Mitch Guttman whose lab will be transitioning to Caltech in the summer of 2013. Candidates must be willing to relocate to California at that time

    Our team recently described a new class of mammalian non-coding genes (lincRNAs) with important roles in human biology and disease. These lincRNAs exert their regulatory influence on the genome in uncharacterized ways. We are looking for a highly motivated individual to join our integrative team to help characterize the function and mechanism of this new class of non-coding gene. This project will include systematic pull-downs of RNA and their associated protein complexes to define lincRNA complexes. In addition, it will include perturbations of lincRNAs and their associated protein complexes to determine their functional roles in embryonic stem cells. This project will make use of various biochemical, molecular biology, and cell biology techniques including (but not limited to) RNA complex purification, in vivo crosslinking methods, RNA-protein immunoprecipitations, RNA-Sequencing, RNA-DNA interaction mapping and embryonic stem cell culture and functional manipulations. Training in established techniques will be provided as needed.


    We are seeing a dependable and creative individual with scientific knowledge, technical expertise, and outstanding practical skills in biochemistry, molecular biology, and cell biology. The candidate will design and perform bench level experiments and small projects that support the overall research objective of an integrative computational and experimental lab in a fast paced and dynamic environment. The successful candidate will possess a broad knowledge of cell biology, molecular biology and/or RNA and protein biochemistry techniques. Techniques will include RNA-Protein crosslinking methods, RNA pull-downs, protein-RNA immunoprecipitations, RNA-protein purification, RNA-DNA interaction mapping, RNA perturbations, and protein detection by quantitative mass-spectrometry. Some techniques will be adaptations of previously established protocols but all will require significant protocol development requiring systematic optimization and troubleshooting. The candidate will adapt easily to changing needs and priorities of a fast-paced research environment, facilitate project completion, address technical problems, determine the methods to be used in research, and summarize and present results.

    Characteristic Duties:
    • Develops new experimental protocols that are essential to the overall project success.
    • Designs and carries out independent projects as well as basic bench level experiments and techniques.
    • Makes detailed observations, analyzes experimental data, and interprets results. Documents and compiles data.
    • Summarizes key data verbally and in writing.
    • Maintains a detailed plan to ensure successful and timely completion of required tasks.
    • Analyzes experimental data and identifies methodological problems. Adjusts experimental protocols to ensure project completion in a timely fashion.
    • Works with team leader to refine experimental protocols and thereby ensure project completion in a timely fashion.
    • Writes and revises molecular biology/biochemistry protocols. Interacts directly with various scientists and other labs.
    • May supervise technical staff and directs experimental plans to ensure overall project success.
    • May be responsible for training of technical staff, graduate students, and post-doctoral fellows as needed.
    • Attends team meetings to share results, plan projects and experiments, provide advice, and to ensure that projects support current team goals in a dynamic environment.
    • Consults with the scientific literature as needed.
    • Other tasks as required.


    • PhD in biology or related field required with up to 3 years postdoctoral experience, or equivalent combination of education and job experience.
    • At least 2 years of supervisory and/or lab management experience strongly preferred.
    • Excellent technical knowledge and hands-on experience with molecular biology methods and RNA and/or protein biochemistry techniques required.
    • Excellent knowledge regarding the operation of standard lab equipment required.
    • Excellent communication skills and the ability to interact professionally with all levels of staff, students, and fellows and with external contacts required.
    • Excellent organization, time management, and project management skills.
    • Ability to handle a variety of tasks simultaneously required.
    • Must be able to use sound judgment to handle a variety of tasks and to shift priorities simultaneously, as well as to effectively solve problems.
    • Ability to solve complex problems.
    • Must be able to work independently as well as part of a team in a fast-paced environment
    • Excellent communication skills and the ability to interact professionally with all levels of staff required.
    • Strong organizational, time management, and project management skills required
    • Creativity, curiosity, and the desire, persistence and ability to create technological advances required
    • Must possess a high level of initiative and the ability to work with little supervision.
    • Experience with RNA or protein biochemistry techniques a plus.
    • Experience with mouse embryonic stem cell culture techniques a plus


    Location : Broad Institute
    Country : United States
    State / Province : Massachusetts
    City : Cambridge


    Disclaimer: immediately upon transition of the Guttman Lab to Caltech, this position will formally terminate at the Broad Institute.


    To apply, please go to:[...]enter

    Req #909

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