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zEST: Gene discovery by EST clusters - Message forums

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LArge clusters Problem
Submitted by Nobody; posted on Monday, May 02, 2005
Hi all, I am trying to cluster 300 K EST's that i got from the NCBI using stackPack. The clutsering give me results totally different from what is present in uniGene. I happen to get 2 large clusters (64k and 34k sequences). If i try to break them by increasing the identity or length of region compared, i get and huge amount of singletons. I was wondering if anyone dealt with such a hassle before ??? Any suggestions will be greately appreciated. Thank you bingo11{AT}hotmail.com



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LArge clusters Problem
Submitted by Nobody; posted on Monday, May 02, 2005
Hi all, I am trying to cluster 300 K EST's that i got from the NCBI using stackPack. The clutsering give me results totally different from what is present in uniGene. I happen to get 2 large clusters (64k and 34k sequences). If i try to break them by increasing the identity or length of region compared, i get and huge amount of singletons. I was wondering if anyone dealt with such a hassle before ??? Any suggestions will be greately appreciated. Thank you bingo11{AT}hotmail.com

 

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