Installation of server (Windows).

CalPred will be installed in-place but it requires some external software be installed in your system. Those are listed below:

- The redistributable microweb server (link).
- The NCBI BLAST-GP program. (link).
- PEPSTATS program of EMBOSSWIN ( download link ) package.
- And svm_classify program of SVMlight ( link ).

These must be installed prior to installation of CalPred server and the users must conform to licenses of these softwares before using them.

Step 1: Uncompress the downloaded CalPred server by using any of the freely avaliable programs such as Winrar / Winzip into "yourfolder"

Step 2: Download the microweb server that comes under non-redistributable license and unzip it.

Step 3 : Now in the CalPred server folder there will be two sub - folders "cgi-bin" and "htdocs" copy the contents of these folders into the same respective folder in microweb server.

Step 4 : BLAST-GP is enhanced BLAST, which supports gaps, developed by the NCBI software group. There is an executable which is necessary for CalPred i.e. blastgp.exe. Please copy "ncbi.ini" file, "data" folder and "blastgp.exe" file into "yourfolder\microweb-1.31\cgi-bin\". Make sure that "ncbi.ini" points to "data" folder's location i.e. edit second line as Data="data".

Step 5: Download EMBOSSWIN and install it on you computer. The EMBOSSWIN is installed by default, in C:/ drive of your computer. If it does'nt, then change the path of installation to "C:\EMBOSSwin". If due to any reasons you are unable to do so, then change these enviornmental variable in "prog.cgi" (in 'yourfolder\microweb-1.31\cgi-bin\') script:

$ENV{'EMBOSSWIN'}="C:/EMBOSSwin"; to $ENV{'EMBOSSWIN'}="your_path ";
$ENV{'EMBOSS_DATA'}="C:/EMBOSSwin/data"; to $ENV{'EMBOSS_DATA'}="your_path/data";
$ENV{'Path'}="C:/EMBOSSwin"; to $ENV{'Path'}="your_path";

Copy "pepstats.exe" from "your_path " to "yourfolder\microweb-1.31\cgi-bin\".

Step 6 : Download SVMlight from its home page (freely available for non-commercial use) and place "svm_classify.exe" into "yourfolder\microweb-1.31\cgi-bin\" directory. If everything goes well, your server should be ready for use now. Start the server using the microweb icon.

Note:

- At the start of server, there might be a security warning in the iexplorer's (if you using it) information bar just right click that bar and check the "Allow this page to access my computer" option. This will redirect you to server homepage.