[BiO BB] from kiran kumar
bioinfo_india at yahoo.com
Tue Nov 20 02:58:51 EST 2001
Toshinori very well explained various methods and
ideas to deal with characterization of end exons.
As he said, Exon I can be a a non-coiding exon in
the a given gene. And thus its a part of 5'UTR.
Traditional cDNA libraries yield, many times
incomplete ends of the cDNA. In such cases one can
either perform 5'RACE or 3'RACE to extend 5' or 3'
But in present day setup many labs prefer to do
insilico analysis and confirm the completeness of a
given gene based on ESTs.
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