[BiO BB] Primer BLAST

Corné HW Klaassen c.klaassen at cwz.nl
Tue Nov 26 03:26:18 EST 2002


Hi Bill,

It sounds to me you want to run a virtual PCR. Try this link:
http://grup.cribi.unipd.it/cgi-bin/mateo/vpcr2.cgi

Hope this helps,

Corné :-)




----- Original Message -----
From: <Bill_A_Nussbaumer at bd.com>
To: <bio_bulletin_board at bioinformatics.org>
Sent: Thursday, November 21, 2002 8:08 PM
Subject: [BiO BB] Primer BLAST


> Hi all,
>
> I'm hoping a method exists that would allow me to align PCR primers
against
> the human genome to determine all products that could be amplified if my
> sample contained extracted human DNA.  Theoretically I could BLAST each
> primer with stringent parameters and record the coordinates on the genome
> looking for matches with a Kb or two of one another.  To do that manually
> would be quite tedious, especially when you consider that I'm only really
> interested in matches at the 3' end.
>
> Anyone know of a way to do this?
>
> - Bill
>
>
>
> _______________________________________________
> BiO_Bulletin_Board maillist  -  BiO_Bulletin_Board at bioinformatics.org
> https://bioinformatics.org/mailman/listinfo/bio_bulletin_board




More information about the BBB mailing list