[BiO BB] Re: PSI-BLAST query filter
kevin_berney at yahoo.com
Thu Mar 10 12:05:27 EST 2005
a clarification perhaps, but doesn't psi-blast give a
list of pairwise alignments to a -- "consensus"
sequence. or maybe a weight matrix?
whereas blast would just give you a list of pairwise
alignments to the original query sequence.
these ought to produce very different results. though
neither is a true multiple alignment.
OK, I still do not understand why blastpgp insists on
sequence... which version are you using?
1) Using blastpgp You won't get a multiple alignment.
You will get a
multiple pairwise alignment to the query sequence.
Using ClustalW you will
get a true multiple alignment.
2) There is still an inherent problem when aligning
3) If those two factors do not matter, then why not
use blastall to build
your alignment? Why do oyu need the iterations
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
More information about the BBB