[BiO BB] Will this implementation of the lac operon work?
kanzure at gmail.com
Thu Feb 28 15:18:54 EST 2008
I am designing a regulatory circuit and have been scratching my head
over how to include enhancers, promoters and TATA boxes, etc. I have
decided to make some progress by randomly guessing and getting feedback
from the community. Here's my work:
Basically I've taken the sequences of BBa_I14032, BBa_R0011, and
BBa_B0034, attached the peptide I want to express, and have at it. It
is my understanding, then, that given a lactose-full environment, my
repressible circuit should express the peptide, and if I wanted to add
a second circuit I could express a repressor that would bond to prevent
RNA polymerase II from manufacturing my peptide as frequently. Correct?
The documentation is rather sparse, so once I get my head around this
I'll be sure to go back and fill in the gaps in the documents.
(I also sent this over to OWW to see what they have to say.)
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