[BiO BB] Newbie question.
drjohn08318 at yahoo.com
drjohn08318 at yahoo.com
Sun Jan 6 19:23:47 EST 2008
Use the upstream seq. You've isolated along with the rec. protein with EMSA.
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-----Original Message-----
From: drjohn08318 at yahoo.com
Date: Mon, 7 Jan 2008 00:20:30
To:"General Forum at Bioinformatics.Org" <bbb at bioinformatics.org>
Subject: Re: [BiO BB] Newbie question.
Hi:
Why not express the protein in E. coli, then carry out EMSA usong the recombinant protein to locate the binding sites on the DNA.
John
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-----Original Message-----
From: "Donnie Darko" <rabidstrikes at googlemail.com>
Date: Sun, 6 Jan 2008 14:57:00
To:bbb at bioinformatics.org
Subject: [BiO BB] Newbie question.
Hi folks,
Suppose I have a novel gene whose product is a DNA binding protein, how
would I go about locating potential binding sites where this protein could
bind? I'm only interested in finding sites for this specific protein and not
a general scan of tfbs.
I've extracted upstream sequences of genes that I'm interested in but I
can't think of a way forward.
Cheers and belated happy new year :-P
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