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<font face="Arial, Helvetica" size=4><b>Gene Quantification Newsletter -
10/2002<br>
October 2002 <br>
<br>
</font><font face="Arial, Helvetica"><u>Introduction:<br>
</u></b>Main focus of the GENE QUANTIFICATION web page
</font><a href="http://www.wzw.tum.de/gene-quantification/" eudora="autourl"><font face="Arial, Helvetica" color="#0000FF"><u>http://www.wzw.tum.de/gene-quantification/</a></u></font><font face="Arial, Helvetica">
is, to describe and summarize all technical aspects involved in
quantitative gene expression analysis using real-time (kinetic) PCR
& RT-PCR: e.g. detection chemistry, assay specificity,
sensitivity, reproducibility, robustness, intra- & inter-assay
variations, kinetic PCR efficiency calculation, quantification
strategies, optimisation strategy, data calculation and statistics,
etc. etc.<br>
<br>
The GENE QUANTIFICATION web page illustrates the usefulness of reliable
quantification strategies, e.g. absolute-, relative-quantification
assays, and the difference between them in kinetic PCR & kinetic
RT-PCR.<br>
<br>
Main focus of the PHYSIOLOGY web page is, to describe all kind of kinetic
RT-PCR applications in molecular Physiology, molecular Endocrinology, and
molecular Immunology (mainly mammary gland immunology) are summarized on
my new homepage
</font><a href="http://www.wzw.tum.de/gene-quantification/physiology.shtml" eudora="autourl"><font face="Arial, Helvetica" color="#0000FF"><u>http://www.wzw.tum.de/gene-quantification/physiology.</a><a href="http://www.wzw.tum.de/gene-quantification/physiology.shtml" eudora="autourl">shtml<br>
<br>
</a></u></font><font face="Arial, Helvetica">#############################################<br>
<br>
</font><font face="Arial, Helvetica" size=4><b>NEW features:<br>
<br>
</font><font face="Arial, Helvetica">Determination of real-time PCR
efficiency</b> - NEW EQUATIONS<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/efficiency.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/efficiency.</a><a href="http://www.wzw.tum.de/gene-quantification/efficiency.html" eudora="autourl">html<br>
<br>
<br>
</a></u></font><font face="Arial, Helvetica"><b>Endocrinology &
Mammary Gland Immunology</b> - NEW PUBLICATIONS<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/physiology.shtml" eudora="autourl">http://www.wzw.tum.de/gene-quantification/physiology.</a><a href="http://www.wzw.tum.de/gene-quantification/physiology.shtml" eudora="autourl">shtml<br>
</a></u></font><font face="Arial, Helvetica">Steroid
receptors: AR, ERa, ERb, PR <br>
Adrenergic receptors: alpha-1-subtypes &
alpha-2-subtypes & beta-subtypes
(publications in press) <br>
Somatotropic axis: GHR, IGF-1, IGF2, both IGF receptors
& IGF-BP1 to BP3 <br>
Serotonin receptors (coming soon)<br>
<br>
<b>NEW MOVIES<br>
</b></font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/movie.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/movie.</a><a href="http://www.wzw.tum.de/gene-quantification/movie.html" eudora="autourl">html<br>
</a></u></font><font face="Arial, Helvetica">e.g. PCR, RPA,
Electrophoresis, Northern- & Southern-Blot<br>
<br>
<br>
<b>NEW PUBLICATIONS about kinetic PCR<br>
</b></font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/real-time.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/real-time.</a><a href="http://www.wzw.tum.de/gene-quantification/real-time.html" eudora="autourl">html<br>
</a></u></font><font face="Arial, Helvetica">and<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/chemistry.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/chemistry.</a><a href="http://www.wzw.tum.de/gene-quantification/chemistry.html" eudora="autourl">html<br>
</a></u></font><font face="Arial, Helvetica">and<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/hkg.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/hkg.</a><a href="http://www.wzw.tum.de/gene-quantification/hkg.html" eudora="autourl">html<br>
</a></u></font><font face="Arial, Helvetica">and<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/literature.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/literature.</a><a href="http://www.wzw.tum.de/gene-quantification/literature.html" eudora="autourl">html<br>
</a></u></font><font face="Arial, Helvetica">sections:<br>
RT-PCR and real-time RT-PCR (in general) <br>
LightCycler specific technical notes <br>
absolute quantification using kinetic PCR <br>
relative quantification using kinetic PCR <br>
classical block RT-PCR
& competitive RT-PCR <br>
normalisation & housekeeping
gene problems in real-time PCR <br>
verification of DNA array results <br>
quantification software: REST,
REST-XL, Q-Gene, geNorm <br>
kinetic RT-PCR poster board<br>
<br>
<br>
<b>Poster boards (a little bit slow !!!):<br>
</b></font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/poster-a.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/poster-a.</a><a href="http://www.wzw.tum.de/gene-quantification/poster-a.html" eudora="autourl">html<br>
</a></u></font><font face="Arial, Helvetica">methodological poster
board A: Array verification <br>
physiological poster board B: IGFs - Early
Response Genes <br>
physiological poster board C: Steroid
receptors <br>
physiological poster board D: Zinc
metabolism <br>
physiological poster board E: Immunological
relevant factors<br>
<br>
<b>New links & databases:<br>
</b></font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/link.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/link.</a><a href="http://www.wzw.tum.de/gene-quantification/link.html" eudora="autourl">html<br>
</a></u></font><font face="Arial, Helvetica">e.g.<br>
- Dictionary of Nucleic Acid Structures (DNAS)<br>
- Glossary iSenseIT<br>
- Molecular Biology Glossary <br>
- A Hypermedia Glossary of Genetic Terms pick from an alphabetical
list of Terms with 656 entries<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.realtimeprimerdatabase.ht.st/" eudora="autourl">http://www.realtimeprimerdatabase.ht.</a><a href="http://www.realtimeprimerdatabase.ht.st/" eudora="autourl">st<br>
</a><a href="http://www.realtimeprimers.org/" eudora="autourl">http://www.realtimeprimers.org/<br>
</a><a href="http://www.pcrlinks.com/" eudora="autourl">http://www.pcrlinks.</a><a href="http://www.pcrlinks.com/" eudora="autourl">com<br>
</a><a href="http://www.cyclertest.com/" eudora="autourl">http://www.cyclertest.com/<br>
</a></u></font><font face="Arial, Helvetica">The COMPLETE Alkami Quick
GuideTM for PCR is a (158 pages)<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf" eudora="autourl">http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.</a><a href="http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf" eudora="autourl">pdf<br>
</a></u></font><font face="Arial, Helvetica">LightCycler University
(LCU)<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/real-time.html#lcu" eudora="autourl">http://www.wzw.tum.de/gene-quantification/real-time.html#</a><a href="http://www.wzw.tum.de/gene-quantification/real-time.html#lcu" eudora="autourl">lcu<br>
<br>
<br>
</a></u></font><font face="Arial, Helvetica"><b>Please send the
NEWSLETTER and the home page links to further scientists and friends who
are interested in real-time PCR, new quantification strategies, relative
or absolute gene quantification using kinetic PCR.<br>
<br>
</b>best regards<br>
<br>
Michael Pfaffl<br>
<br>
#############################################<br>
<br>
</font><font face="Arial, Helvetica" size=1>For further QUESTIONS
concerning real-time PCR and if you want to SUBSCRIBE to our monthly
appearing GENE QUANTIFICATION NEWSLETTER please contact
</font><font face="Arial, Helvetica" size=1 color="#0000FF"><u>gene.quantification@wzw.tum.de</u></font><font face="Arial, Helvetica" size=1>
You will receive all NEW FEATURES and future REST & REST-XL
updates.<br>
<br>
GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are
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********************************************************************<br>
<font size=5><b>Gene Quantification @ wzw.tum.de<br>
<br>
</b></font><font color="#0000FF">e-mail:<x-tab> </x-tab>gene.quantification@wzw.tum.de
<br>
homepage:<x-tab> </x-tab><a href="http://www.wzw.tum.de/gene-quantification/index.shtml" eudora="autourl">http://www.wzw.tum.de/gene-quantification/index.shtml</a><br>
homepage:<x-tab> </x-tab><a href="http://www.wzw.tum.de/gene-quantification/physiology.shtml" eudora="autourl">http://www.wzw.tum.de/gene-quantification/physiology.shtml</a><br>
<br>
</font><u>responsible Senior Scientist:<br>
</u>Dr. Michael W. Pfaffl<br>
Institute of Physiology<br>
Life Science Center Weihenstephan,<br>
Technical University of Munich<br>
Weihenstephaner Berg 3<br>
D-85350 Freising-Weihenstephan<br>
GERMANY<br>
<br>
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