hi, has anyone calculated divergence time for a pair of sequence. its like say there are 10 sequences and after doing multiple alignment and phylogenetic tree construction(say NJ), if i have to find out the divergence time for a pair of sequence(xyz million years), how to go about it.. i tried some software like mega, JaDis but i was not able to interpret the results properly (there was some confusion in mega and the help was rather insufficient), if anyone has any idea on this, kindly share your views of doing this. thanks for ur response on phylogenetic sw, infact i was using mega but i will defnitely give a try for the one yadhu suggested. thanks, balaji Enjoy the little things in life,for one day you may look back and realize they were the big things. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Knowledge is Power, and Power is Respect, With the Knowledge we learn TODAY may we Earn the Respect (and Money) for TOMORROW!!! Peace & Love be with you...