From farhat.habib at gmail.com Wed Mar 9 09:24:31 2011 From: farhat.habib at gmail.com (Farhat Habib) Date: Wed, 9 Mar 2011 19:54:31 +0530 Subject: [Bio-Linux] Error while updating Message-ID: Dear All, On trying to update using the Package manager I am running into this error. W: GPG error: http://nebc.nerc.ac.uk unstable Release: The following signatures were invalid: BADSIG B1A598E8128B92BD NEBC Team (Bio-Linux 6 package signing key) W: A error occurred during the signature verification. The repository is not updated and the previous index files will be used.GPG error: http://www.stats.bris.ac.uk lucid/ Release: The following signatures were invalid: BADSIG 51716619E084DAB9 Michael Rutter What should I do? Thanks, -Farhat -- Farhat Habib, PhD Scientist-C, Center of Excellence in Epigenetics Indian Institute of Science Education & Research, Sai Trinity Building, Garware Circle, Sutarwadi, Pashan, Pune-411 021. http://www.iiserpune.ac.in/~farhat Twitter: @far_hat Office: +91 20 2590 8080 -------------- next part -------------- An HTML attachment was scrubbed... URL: From makson96 at gmail.com Wed Mar 9 09:53:51 2011 From: makson96 at gmail.com (Tomasz Makarewicz) Date: Wed, 9 Mar 2011 15:53:51 +0100 Subject: [Bio-Linux] Error while updating In-Reply-To: References: Message-ID: http://lmgtfy.com/?q=ubuntu+gpg+error 2011/3/9 Farhat Habib > Dear All, > > On trying to update using the Package manager I am running into this > error. > > > W: GPG error: http://nebc.nerc.ac.uk unstable Release: The following > signatures were invalid: BADSIG B1A598E8128B92BD NEBC Team (Bio-Linux 6 > package signing key) > W: A error occurred during the signature verification. The repository is > not updated and the previous index files will be used.GPG error: > http://www.stats.bris.ac.uk lucid/ Release: The following signatures were > invalid: BADSIG 51716619E084DAB9 Michael Rutter > > What should I do? > > Thanks, > -Farhat > > -- > Farhat Habib, PhD > Scientist-C, Center of Excellence in Epigenetics > Indian Institute of Science Education & Research, > Sai Trinity Building, Garware Circle, > Sutarwadi, Pashan, Pune-411 021. > http://www.iiserpune.ac.in/~farhat > Twitter: @far_hat > Office: +91 20 2590 8080 > > > _______________________________________________ > Bio-Linux mailing list > Bio-Linux at nebclists.nerc.ac.uk > http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux > > -- http://ubuntu.pl -------------- next part -------------- An HTML attachment was scrubbed... URL: From mashanovvlad at googlemail.com Wed Mar 9 09:59:09 2011 From: mashanovvlad at googlemail.com (vladimir mashanov) Date: Wed, 9 Mar 2011 10:59:09 -0400 Subject: [Bio-Linux] Error while updating In-Reply-To: References: Message-ID: Dear Farhat, I had the same problem too. Below is the reply from Tim Booth that helped in my case: The README should answer your question: http://cran.r-project.org/bin/linux/ubuntu/README Best, Vladimir. On 9 March 2011 10:24, Farhat Habib wrote: > Dear All, > On trying to update using the Package manager I am running into this error. > > W: GPG error: http://nebc.nerc.ac.uk unstable Release: The following > signatures were invalid: BADSIG B1A598E8128B92BD NEBC Team (Bio-Linux 6 > package signing key) > W: A error occurred during the signature verification. The repository is not > updated and the previous index files will be used.GPG error: > http://www.stats.bris.ac.uk lucid/ Release: The following signatures were > invalid: BADSIG 51716619E084DAB9 Michael Rutter > What should I do? > Thanks, > -Farhat > -- > Farhat Habib, PhD > Scientist-C, Center of Excellence in Epigenetics > Indian Institute of Science Education & Research, > Sai Trinity Building, Garware Circle, > Sutarwadi, Pashan, Pune-411 021. > http://www.iiserpune.ac.in/~farhat > Twitter: @far_hat > Office: +91 20 2590 8080 > > _______________________________________________ > Bio-Linux mailing list > Bio-Linux at nebclists.nerc.ac.uk > http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux > > -- Vladimir Mashanov, PhD Universidad de Puerto Rico Faculdad de Ciencias Naturales Departamento de Biologia JGD Building, #220 PO Box 70377, UPR Station Rio Piedras, PR 00936-8377 email: mashanovvlad at googlemail.com; vladimir.mashanov at uprrp.edu From farhat.habib at gmail.com Wed Mar 9 10:08:02 2011 From: farhat.habib at gmail.com (Farhat Habib) Date: Wed, 9 Mar 2011 20:38:02 +0530 Subject: [Bio-Linux] Error while updating In-Reply-To: References: Message-ID: Many thanks! -Farhat -- Farhat Habib, PhD Scientist-C, Center of Excellence in Epigenetics Indian Institute of Science Education & Research, Sai Trinity Building, Garware Circle, Sutarwadi, Pashan, Pune-411 021. http://www.iiserpune.ac.in/~farhat Twitter: @far_hat Office: +91 20 2590 8080 On Wed, Mar 9, 2011 at 8:23 PM, Tomasz Makarewicz wrote: > http://lmgtfy.com/?q=ubuntu+gpg+error > > 2011/3/9 Farhat Habib > >> Dear All, >> >> On trying to update using the Package manager I am running into this >> error. >> >> >> W: GPG error: http://nebc.nerc.ac.uk unstable Release: The following >> signatures were invalid: BADSIG B1A598E8128B92BD NEBC Team (Bio-Linux 6 >> package signing key) >> W: A error occurred during the signature verification. The repository is >> not updated and the previous index files will be used.GPG error: >> http://www.stats.bris.ac.uk lucid/ Release: The following signatures were >> invalid: BADSIG 51716619E084DAB9 Michael Rutter >> >> What should I do? >> >> Thanks, >> -Farhat >> >> -- >> Farhat Habib, PhD >> Scientist-C, Center of Excellence in Epigenetics >> Indian Institute of Science Education & Research, >> Sai Trinity Building, Garware Circle, >> Sutarwadi, Pashan, Pune-411 021. >> http://www.iiserpune.ac.in/~farhat >> Twitter: @far_hat >> Office: +91 20 2590 8080 >> >> >> _______________________________________________ >> Bio-Linux mailing list >> Bio-Linux at nebclists.nerc.ac.uk >> http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux >> >> > > > -- > http://ubuntu.pl > > _______________________________________________ > Bio-Linux mailing list > Bio-Linux at nebclists.nerc.ac.uk > http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux > > -------------- next part -------------- An HTML attachment was scrubbed... URL: From tbooth at ceh.ac.uk Wed Mar 30 12:55:28 2011 From: tbooth at ceh.ac.uk (Tim Booth) Date: Wed, 30 Mar 2011 17:55:28 +0100 Subject: [Bio-Linux] FastQC tool for checking high throughput sequence data Message-ID: <1301504128.1947.3814.camel@barsukas> Hi All, Today we've been playing with the FastQC tool from the Babraham institute. It takes the .fastq file you get from an Illumina run and makes some nice reports and graphs to let you visualise various metrics for your sequences and assess the quality. Downloading and running the tool is fairly easy but I've made a Bio-Linux package that comes with the added convenience of an icon appearing in the Bioinformatics menu, so you may want to use that instead: apt-get install bio-linux-fastqc We had some 454 data to analyse and it was saved as separate .fasta and .qual files which needed to be converted to a single .fastq file for FastQC. If you need to do the same, there are some handy conversion scripts now on the website: http://nebc.nerc.ac.uk/tools/code-corner/scripts/sequence-formatting-and-other-text-manipulation Cheers, TIM -- Tim Booth NERC Environmental Bioinformatics Centre Centre for Ecology and Hydrology Maclean Bldg, Benson Lane Crowmarsh Gifford Wallingford, England OX10 8BB http://nebc.nerc.ac.uk +44 1491 69 2705 -- This message (and any attachments) is for the recipient only. NERC is subject to the Freedom of Information Act 2000 and the contents of this email and any reply you make may be disclosed by NERC unless it is exempt from release under the Act. Any material supplied to NERC may be stored in an electronic records management system.