<div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">dear sir,</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
i am Dr.VIJAY using BIOLINUX 7 for last 2 months.its really working good for biologist with own PC. now i face problem while working with BLAST run in terminal. when ever i run the BLAST i am getting error message like below for both single sequences and multiple sequences too. please help me( i am biology student, so i am not exposure to linux commend and error problem.THANK U SIR</div>
<div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"><br></div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
<br></div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">vijayakumar@vijayakumar-SVE14112ENB[bioinf_files] blastx -db blastdb/nr.02 -query sra_data contig list - nucleotide_ sequences.fasta -evalue 0.1 > vijay.blastx</div>
<div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">USAGE</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
blastx [-h] [-help] [-import_search_strategy filename]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-export_search_strategy filename] [-db database_name]</div>
<div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-dbsize num_letters] [-gilist filename] [-seqidlist filename]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
[-negative_gilist filename] [-entrez_query entrez_query]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-db_soft_mask filtering_algorithm] [-db_hard_mask filtering_algorithm]</div>
<div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-subject subject_input_file] [-subject_loc range] [-query input_file]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
[-out output_file] [-evalue evalue] [-word_size int_value]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-gapopen open_penalty] [-gapextend extend_penalty]</div>
<div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-xdrop_ungap float_value] [-xdrop_gap float_value]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
[-xdrop_gap_final float_value] [-searchsp int_value]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-max_hsps_per_subject int_value] [-frame_shift_penalty frameshift]</div>
<div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-max_intron_length length] [-seg SEG_options]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
[-soft_masking soft_masking] [-matrix matrix_name]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-threshold float_value] [-culling_limit int_value]</div>
<div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-best_hit_overhang float_value] [-best_hit_score_edge float_value]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
[-window_size int_value] [-ungapped] [-lcase_masking] [-query_loc range]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-strand strand] [-parse_deflines] [-query_gencode int_value]</div>
<div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-outfmt format] [-show_gis] [-num_descriptions int_value]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
[-num_alignments int_value] [-html] [-max_target_seqs num_sequences]</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> [-num_threads int_value] [-remote] [-version]</div>
<div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"><br></div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
DESCRIPTION</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)"> Translated Query-Protein Subject BLAST 2.2.26+</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
<br></div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">Use '-help' to print detailed descriptions of command line arguments</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
========================================================================</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">Error: (CArgException::eSynopsis) Too many positional arguments (1), the offending value: contig</div>
<div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">Error: (CArgException::eSynopsis) Application's initialization failed</div><div style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:13px;background-color:rgb(255,255,255)">
vijayakumar@vijayakumar-SVE14112ENB[bioinf_files]</div>