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<DIV>Dear Experts,</DIV>
<DIV>I installed last version of Bio (on February) as a dual setup on a computer.</DIV>
<DIV>I need to work with data of RNA-Seq. Experiments were done on HiSeq2500 unit. I have all data, they are all right. I downloaded Bio-Linux fron DVD<BR>disk, installed on a computer, with 8G RAM, 4 core processor and 128 G disk space. I did update as recommended, all went fine.</DIV>
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<DIV>1. I am using NGS programs, but problems start from very beginning, I can not upload files, (they are big~3GB), when I press "execute" panel with<BR>arrow blinks, (on right pane) but nothing is happening and it may continue for 48 h! If I use (usegalaxy.org with the same file all is all right.) I can<BR>upload small files ~.5G but,</DIV>
<DIV>2. If file is small I can not run it because when I need to chose reference geneome, in option "GENOME", it doesn't work-nothing is in the frame, (when I upload file option GENOME works fine). Also in some NGS programs option to choose file doesn't work. It doesn't see file at all. </DIV>
<DIV>As far as I can see (I am a molecular biologist, not a IT tech) this is the problem of installation. I tried to read manuals, but it sad open file XYZ.loc (or ABC.ini) ore something like this, which I can not fined at all, nothing like this.</DIV>
<DIV>Any suggestions?</DIV>
<DIV>Thanks</DIV>
<DIV>Andrew</DIV>
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<DIV>Can you explain what's wrong?</DIV>
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<DIV>-----------------------------<BR>Andrew Zlobin, Ph.D<BR>Cancer Center, Loyola University Medical Center<BR>2160 S. First Ave, Bldg 112,</DIV>
<DIV>Rm 323,<BR>Maywood, IL 60153<BR>(708) 327-3122 </DIV>
<DIV>(708) 327-3342 fax<BR>mailto:azlobin@lumc.edu <BR></DIV></BODY></HTML>