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<div>Hi All,</div>
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<div>we’re using live USBs for a course next week that Tim kindly cloned after installing a couple of R packages and a test dataset. He’s now on his hols, so I thought I’d check to see whether the rest of the list could help me with an issue that I’m having.</div>
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<div>On boot, I couldn’t find the data that Tim added. I’ve checked by creating a file and the sticks aren’t persistent and files are removed when the USB stick is. I did find a reference that suggested creating a new user and that user account would be persistent,
but no joy, new user and file removed on reboot.</div>
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<div>The additional R packages that Tim added to the distribution (dev version of PhyloSeq) are present and correct, so that at least has stayed. </div>
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<div>I have checked in:</div>
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<div>/syslinux/txt.cfg </div>
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<div>and this seems correct - the persistent flag is set on the Try Bio-Linux 7 option:</div>
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<div>default live<br>
label live<br>
menu label ^Try Bio-Linux 7 without installing<br>
kernel /casper/vmlinuz.efi<br>
append noprompt cdrom-detect/try-usb=true persistent file=/cdrom/preseed/ubuntu.seed boot=casper initrd=/casper/initrd.lz quiet splash locale=en_GB.UTF-8 --<br>
label live-install<br>
menu label ^Install Bio-Linux 7<br>
kernel /casper/vmlinuz.efi<br>
append noprompt cdrom-detect/try-usb=true persistent file=/cdrom/preseed/ubuntu.seed boot=casper only-ubiquity initrd=/casper/initrd.lz quiet splash --<br>
label check<br>
menu label ^Check stick for defects<br>
kernel /casper/vmlinuz.efi<br>
append boot=casper noprompt integrity-check initrd=/casper/initrd.lz quiet splash --<br>
label memtest<br>
menu label Test ^memory<br>
kernel /install/mt86plus<br>
label hd<br>
menu label ^Boot from first hard disk<br>
localboot 0x80</div>
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<div>This post <a href="http://ubuntuforums.org/showthread.php?t=2124124&p=12551088#post12551088">http://ubuntuforums.org/showthread.php?t=2124124&p=12551088#post12551088</a> suggests editing syslinux.cfg (rather than txt.cfg) to make a Ubuntu stick persistent,
which I’ve tried, but it booted up exactly as before and again was stubbornly resistant to retaining files.</div>
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<div>Can anyone suggest ways to get this working? Given the fact the R packages have remained, some aspect of this seems to be functioning? I could have the attendees download the data and course material, but would much rather they left with a copy of Bio-Linux
and all their work and notes in one elegant package. </div>
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<div>Thanks very much for your help</div>
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<div>Mike</div>
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Mike Cox<br>
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Research Associate<br>
Molecular Genetics & Genomics</div>
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<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">
Genomic Medicine<br>
National Heart and Lung Institute<br>
Guy Scadding Building<br>
Royal Brompton Campus<br>
London SW3 6LY<br>
<br>
0207 594 7974</div>
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<br>
<a href="http://www1.imperial.ac.uk/medicine/people/michael.cox1/">http://www1.imperial.ac.uk/medicine/people/michael.cox1/</a></div>
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* New NHLI short course "Principles of Genomic Medicine" *<br>
<a href="http://www1.imperial.ac.uk/nhli/PGM">http://www1.imperial.ac.uk/nhli/PGM</a><br>
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