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<div class="moz-cite-prefix">Dear All, <br>
<br>
Just for your information, their is an alternative way to create
Bio-Linux live USB drives. Universal usb installer allows you to
create live usb for any Linux os in minutes. <br>
<br>
For Bio-Linux just select the option for Ubuntu 64 bit. I have
several bio-Linux live USB made with tool and never had any
issues with it. <br>
<br>
You can access the tool for free here: <br>
<a class="moz-txt-link-freetext" href="http://www.pendrivelinux.com/universal-usb-installer-easy-as-1-2-3/">http://www.pendrivelinux.com/universal-usb-installer-easy-as-1-2-3/</a><br>
<br>
Regards, <br>
James<br>
<br>
On 6/12/2014 4:12 AM, Cox, Michael J wrote:<br>
</div>
<blockquote
cite="mid:A071F354-4A38-4C04-A8DA-65C6CB2DB173@imperial.ac.uk"
type="cite">
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Dear All,
<div><br>
</div>
<div>I think I fixed this, when I checked /boot/grub/grub.cfg
there was no persistent flag. Adding this to the first menu
entry line:</div>
<div><br>
</div>
<div>linux /casper/vmlinuz.efi file=/cdrom/preseed/ubuntu.seed
boot=casper <b>persistent</b> quite splash —</div>
<div><br>
</div>
<div>now renders the sticks persistent. In addition I can now see
the data and tutorials that Tim loaded for me in /home. </div>
<div><br>
</div>
<div>It does take a little longer to load and is slow initially,
so I have some concerns that I have done something less than
ideal. Comments appreciated.</div>
<div><br>
</div>
<div>Thanks</div>
<div><br>
</div>
<div>Mike</div>
<div>
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<div>On 12 Jun 2014, at 12:00, <<a moz-do-not-send="true"
href="mailto:bio-linux-request@nebclists.nerc.ac.uk">bio-linux-request@nebclists.nerc.ac.uk</a>>
<<a moz-do-not-send="true"
href="mailto:bio-linux-request@nebclists.nerc.ac.uk">bio-linux-request@nebclists.nerc.ac.uk</a>>
wrote:</div>
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Today's Topics:<br>
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1. Live Bio-Linux not persistent (Cox, Michael J)<br>
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<br>
----------------------------------------------------------------------<br>
<br>
Message: 1<br>
Date: Thu, 12 Jun 2014 09:13:37 +0000<br>
From: "Cox, Michael J" <a class="moz-txt-link-rfc2396E" href="mailto:michael.cox1@imperial.ac.uk"><michael.cox1@imperial.ac.uk></a><br>
To: <a class="moz-txt-link-rfc2396E" href="mailto:bio-linux@nebclists.nerc.ac.uk">"bio-linux@nebclists.nerc.ac.uk"</a>
<a class="moz-txt-link-rfc2396E" href="mailto:bio-linux@nebclists.nerc.ac.uk"><bio-linux@nebclists.nerc.ac.uk></a><br>
Subject: [Bio-Linux] Live Bio-Linux not persistent<br>
Message-ID:
<a class="moz-txt-link-rfc2396E" href="mailto:B6B32EFF-5AD6-436B-B760-975F4CCA4864@imperial.ac.uk"><B6B32EFF-5AD6-436B-B760-975F4CCA4864@imperial.ac.uk></a><br>
Content-Type: text/plain; charset="windows-1252"<br>
<br>
Hi All,<br>
<br>
we?re using live USBs for a course next week that Tim kindly
cloned after installing a couple of R packages and a test
dataset. He?s now on his hols, so I thought I?d check to
see whether the rest of the list could help me with an issue
that I?m having.<br>
<br>
On boot, I couldn?t find the data that Tim added. I?ve
checked by creating a file and the sticks aren?t persistent
and files are removed when the USB stick is. I did find a
reference that suggested creating a new user and that user
account would be persistent, but no joy, new user and file
removed on reboot.<br>
<br>
The additional R packages that Tim added to the distribution
(dev version of PhyloSeq) are present and correct, so that
at least has stayed.<br>
<br>
I have checked in:<br>
<br>
/syslinux/txt.cfg<br>
<br>
and this seems correct - the persistent flag is set on the
Try Bio-Linux 7 option:<br>
<br>
default live<br>
label live<br>
menu label ^Try Bio-Linux 7 without installing<br>
kernel /casper/vmlinuz.efi<br>
append noprompt cdrom-detect/try-usb=true persistent
file=/cdrom/preseed/ubuntu.seed boot=casper
initrd=/casper/initrd.lz quiet splash locale=en_GB.UTF-8 --<br>
label live-install<br>
menu label ^Install Bio-Linux 7<br>
kernel /casper/vmlinuz.efi<br>
append noprompt cdrom-detect/try-usb=true persistent
file=/cdrom/preseed/ubuntu.seed boot=casper only-ubiquity
initrd=/casper/initrd.lz quiet splash --<br>
label check<br>
menu label ^Check stick for defects<br>
kernel /casper/vmlinuz.efi<br>
append boot=casper noprompt integrity-check
initrd=/casper/initrd.lz quiet splash --<br>
label memtest<br>
menu label Test ^memory<br>
kernel /install/mt86plus<br>
label hd<br>
menu label ^Boot from first hard disk<br>
localboot 0x80<br>
<br>
This post
<a class="moz-txt-link-freetext" href="http://ubuntuforums.org/showthread.php?t=2124124&p=12551088#post12551088">http://ubuntuforums.org/showthread.php?t=2124124&p=12551088#post12551088</a>
suggests editing syslinux.cfg (rather than txt.cfg) to make
a Ubuntu stick persistent, which I?ve tried, but it booted
up exactly as before and again was stubbornly resistant to
retaining files.<br>
<br>
Can anyone suggest ways to get this working? Given the fact
the R packages have remained, some aspect of this seems to
be functioning? I could have the attendees download the
data and course material, but would much rather they left
with a copy of Bio-Linux and all their work and notes in one
elegant package.<br>
<br>
Thanks very much for your help<br>
<br>
Mike<br>
<br>
<br>
Mike Cox<br>
<br>
Research Associate<br>
Molecular Genetics & Genomics<br>
<br>
Genomic Medicine<br>
National Heart and Lung Institute<br>
Guy Scadding Building<br>
Royal Brompton Campus<br>
London SW3 6LY<br>
<br>
0207 594 7974<br>
<br>
<a class="moz-txt-link-freetext" href="http://www1.imperial.ac.uk/medicine/people/michael.cox1/">http://www1.imperial.ac.uk/medicine/people/michael.cox1/</a><br>
<br>
* New NHLI short course "Principles of Genomic Medicine" *<br>
<a class="moz-txt-link-freetext" href="http://www1.imperial.ac.uk/nhli/PGM">http://www1.imperial.ac.uk/nhli/PGM</a><br>
<br>
<br>
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