<div dir="ltr">Thanks again for the upgrade. I&#39;m already running Trinity for 2 hours, and it is now on Chrysalis phase. So, I&#39;m assuming Inchworm part is now OK. I&#39;ll let you know if it stumbles on another point.<br></div><div class="gmail_extra"><br><div class="gmail_quote">On Thu, Oct 29, 2015 at 7:59 AM, Tim Booth <span dir="ltr">&lt;<a href="mailto:tbooth@ceh.ac.uk" target="_blank">tbooth@ceh.ac.uk</a>&gt;</span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Hi Carlos,<br>
<br>
Thanks for being the first proper tester on the Trinity package.  As<br>
with Jellyfish the package was building correctly (hence passing<br>
internal tests) but Inchworm never got packed into the final DEB file.<br>
I&#39;ve fixed the build rules and the new version is preparing on Launchpad<br>
now.  Should appear as an update in about an hour.<br>
<br>
Cheers,<br>
<br>
TIM<br>
<br>
On Wed, 2015-10-28 at 16:34 -0200, Carlos Morais wrote:<br>
&gt; Thanks a lot, Tim!<br>
&gt;<br>
&gt; This upgrade really fixes the Jellyfish part.<br>
&gt;<br>
&gt; Now it breaks on Inchworm.<br>
&gt;<br>
&gt; Apparently, trinity neither include Inchworm nor depends on another<br>
&gt; package providing it. :-(<br>
&gt;<br>
&gt; bash: /usr/lib/trinityrnaseq/Inchworm/bin//inchworm: No such file or<br>
&gt; directory<br>
&gt;<br>
&gt;<br>
&gt; Cheers,<br>
&gt;<br>
&gt;     Carlos<br>
&gt;<br>
&gt;<br>
&gt; On Tue, Oct 27, 2015 at 3:31 PM, Tim Booth &lt;<a href="mailto:tbooth@ceh.ac.uk">tbooth@ceh.ac.uk</a>&gt; wrote:<br>
&gt;         Hi Carlos,<br>
&gt;<br>
&gt;         This looks to me like someone (maybe me) stuffed up the<br>
&gt;         jellyfish<br>
&gt;         package and installed the wrong file into /usr/bin.  It seems<br>
&gt;         to be a<br>
&gt;         simple typo in the build script.<br>
&gt;<br>
&gt;         I&#39;ve amended the package and uploaded it to Launchpad.  Please<br>
&gt;         look out<br>
&gt;         for the updated package 2.2.3-2biolinux2 and see if that sorts<br>
&gt;         it.<br>
&gt;<br>
&gt;         Cheers,<br>
&gt;<br>
&gt;         TIM<br>
&gt;<br>
&gt;         On Mon, 2015-10-26 at 08:41 -0200, Carlos Morais wrote:<br>
&gt;         &gt; Anybody else experienced this error? Any ideas?<br>
&gt;         &gt; Thanks<br>
&gt;         &gt;<br>
&gt;         &gt; Em 15/10/2015 12:28, &quot;Carlos Morais&quot; &lt;<a href="mailto:morais@iq.usp.br">morais@iq.usp.br</a>&gt;<br>
&gt;         escreveu:<br>
&gt;         &gt;         Thanks a lot for the update.<br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;         &gt;         However, it is trying to compile Jellyfish on the<br>
&gt;         go. I&#39;m<br>
&gt;         &gt;         attaching the output. Relevant part:<br>
&gt;         &gt;<br>
&gt;         &gt;         Trinity version: v2.0.6<br>
&gt;         &gt;         ...<br>
&gt;         &gt;         -------------------------------------------<br>
&gt;         &gt;         ----------- Jellyfish  --------------------<br>
&gt;         &gt;         -- (building a k-mer catalog from reads) --<br>
&gt;         &gt;         -------------------------------------------<br>
&gt;         &gt;<br>
&gt;         &gt;         Wednesday, October 14, 2015: 13:46:18<br>
&gt;         &gt;         CMD: /usr/bin/jellyfish count -t 15 -m 25 -s<br>
&gt;         14446126223<br>
&gt;         &gt;         --canonical  both.fa<br>
&gt;         &gt;         mkdir: cannot create directory ‘/usr/bin/.libs’:<br>
&gt;         Permission<br>
&gt;         &gt;         denied<br>
&gt;         &gt;         /usr/bin/jellyfish: line 202:<br>
&gt;         &gt;         cd: /build/jellyfish-sMXWdl/jellyfish-2.2.3: No such<br>
&gt;         file or<br>
&gt;         &gt;         directory<br>
&gt;         &gt;         g++: error: sub_commands/jellyfish.o: No such file<br>
&gt;         or<br>
&gt;         &gt;         directory<br>
&gt;         &gt;         g++: error: sub_commands/count_main.o: No such file<br>
&gt;         or<br>
&gt;         &gt;         directory<br>
&gt;         &gt;         g++: error: sub_commands/info_main.o: No such file<br>
&gt;         or<br>
&gt;         &gt;         directory<br>
&gt;         &gt;         g++: error: sub_commands/dump_main.o: No such file<br>
&gt;         or<br>
&gt;         &gt;         directory<br>
&gt;         &gt;         g++: error: sub_commands/histo_main.o: No such file<br>
&gt;         or<br>
&gt;         &gt;         directory<br>
&gt;         &gt;         g++: error: sub_commands/stats_main.o: No such file<br>
&gt;         or<br>
&gt;         &gt;         directory<br>
&gt;         &gt;         g++: error: sub_commands/merge_main.o: No such file<br>
&gt;         or<br>
&gt;         &gt;         directory<br>
&gt;         &gt;         g++: error: sub_commands/bc_main.o: No such file or<br>
&gt;         directory<br>
&gt;         &gt;         g++: error: sub_commands/query_main.o: No such file<br>
&gt;         or<br>
&gt;         &gt;         directory<br>
&gt;         &gt;         g++: error: sub_commands/cite_main.o: No such file<br>
&gt;         or<br>
&gt;         &gt;         directory<br>
&gt;         &gt;         g++: error: sub_commands/mem_main.o: No such file or<br>
&gt;         directory<br>
&gt;         &gt;         g++: error: jellyfish/merge_files.o: No such file or<br>
&gt;         directory<br>
&gt;         &gt;         g++: error: ./.libs/<a href="http://libjellyfish-2.0.so" rel="noreferrer" target="_blank">libjellyfish-2.0.so</a>: No such<br>
&gt;         file or<br>
&gt;         &gt;         directorynError, cmd: /usr/bin/jellyfish count -t 15<br>
&gt;         -m 25 -s<br>
&gt;         &gt;         14446126223  --canonical  both.fa died with ret 256<br>
&gt;         &gt;         at /usr/lib/trinityrnaseq/Trinity line 2115.<br>
&gt;         &gt;<br>
&gt;         &gt;         Trinity run failed. Must investigate error above.<br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;         &gt;         On Mon, Sep 14, 2015 at 10:00 AM, Bishwa Kiran<br>
&gt;         &gt;         &lt;<a href="mailto:kirannbishwa01@gmail.com">kirannbishwa01@gmail.com</a>&gt; wrote:<br>
&gt;         &gt;                 Thanks for the update !<br>
&gt;         &gt;<br>
&gt;         &gt;                 On Mon, Sep 14, 2015 at 6:42 AM, Tim Booth<br>
&gt;         &gt;                 &lt;<a href="mailto:tbooth@ceh.ac.uk">tbooth@ceh.ac.uk</a>&gt; wrote:<br>
&gt;         &gt;                         Hi All,<br>
&gt;         &gt;<br>
&gt;         &gt;                         I&#39;ve been doing a round of package<br>
&gt;         updates on<br>
&gt;         &gt;                         Bio-Linux 8 over the last<br>
&gt;         &gt;                         week.  We now have both Mothur and<br>
&gt;         Mira<br>
&gt;         &gt;                         updated to the latest versions:<br>
&gt;         &gt;<br>
&gt;         &gt;                         mira-assembler package is version<br>
&gt;         4.9.5<br>
&gt;         &gt;                         mothur package is version 1.36.1<br>
&gt;         &gt;<br>
&gt;         &gt;                         Both will be updated by the package<br>
&gt;         updater.<br>
&gt;         &gt;                         If you experience any<br>
&gt;         &gt;                         problems or regressions with these<br>
&gt;         new<br>
&gt;         &gt;                         packages please let me know.<br>
&gt;         &gt;<br>
&gt;         &gt;                         I&#39;m also pleased to say that the<br>
&gt;         Trinity<br>
&gt;         &gt;                         RNA-Seq De novo assembler has<br>
&gt;         &gt;                         been added to the package repository<br>
&gt;         &gt;                         (<a href="http://trinityrnaseq.github.io/" rel="noreferrer" target="_blank">http://trinityrnaseq.github.io/</a>).<br>
&gt;         &gt;                         Various modifications have been made<br>
&gt;         to<br>
&gt;         &gt;                         Trinity to make it integrate<br>
&gt;         &gt;                         nicely with the other packages on<br>
&gt;         the system,<br>
&gt;         &gt;                         so if you have problems<br>
&gt;         &gt;                         running it please let me know and<br>
&gt;         I&#39;ll check<br>
&gt;         &gt;                         if it is maybe something<br>
&gt;         &gt;                         that got messed up in the packaging.<br>
&gt;         &gt;<br>
&gt;         &gt;                         trinityrnaseq package is version<br>
&gt;         2.0.6<br>
&gt;         &gt;<br>
&gt;         &gt;                         To get it: sudo apt-get install<br>
&gt;         trinityrnaseq<br>
&gt;         &gt;                         then just type: &quot;Trinity&quot; (with the<br>
&gt;         capital T)<br>
&gt;         &gt;<br>
&gt;         &gt;                         The credit for all this goes mainly<br>
&gt;         to Michael<br>
&gt;         &gt;                         and Andreas from the<br>
&gt;         &gt;                         Debian Med project who maintain<br>
&gt;         these packages<br>
&gt;         &gt;                         in Debian.  I&#39;ve been<br>
&gt;         &gt;                         able to take what they made and<br>
&gt;         backport it to<br>
&gt;         &gt;                         Bio-Linux for you.<br>
&gt;         &gt;<br>
&gt;         &gt;                         Cheers,<br>
&gt;         &gt;<br>
&gt;         &gt;                         TIM<br>
&gt;         &gt;<br>
&gt;         &gt;                         --<br>
&gt;         &gt;                         Tim Booth &lt;<a href="mailto:tbooth@ceh.ac.uk">tbooth@ceh.ac.uk</a>&gt;<br>
&gt;         &gt;<br>
&gt;         &gt;                         Centre for Ecology and Hydrology<br>
&gt;         &gt;                         Maclean Bldg, Benson Lane<br>
&gt;         &gt;                         Crowmarsh Gifford<br>
&gt;         &gt;                         Wallingford, England<br>
&gt;         &gt;                         OX10 8BB<br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;          <a href="http://environmentalomics.org/bio-linux" rel="noreferrer" target="_blank">http://environmentalomics.org/bio-linux</a><br>
&gt;         &gt;                         <a href="tel:%2B44%201491%2069%202297" value="+441491692297">+44 1491 69 2297</a><br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;          _______________________________________________<br>
&gt;         &gt;                         Bio-Linux mailing list<br>
&gt;         &gt;                         <a href="mailto:Bio-Linux@nebclists.nerc.ac.uk">Bio-Linux@nebclists.nerc.ac.uk</a><br>
&gt;         &gt;<br>
&gt;          <a href="http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux" rel="noreferrer" target="_blank">http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux</a><br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;         &gt;                 --<br>
&gt;         &gt;                 - Bish<br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;          _______________________________________________<br>
&gt;         &gt;                 Bio-Linux mailing list<br>
&gt;         &gt;                 <a href="mailto:Bio-Linux@nebclists.nerc.ac.uk">Bio-Linux@nebclists.nerc.ac.uk</a><br>
&gt;         &gt;<br>
&gt;          <a href="http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux" rel="noreferrer" target="_blank">http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux</a><br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;         &gt;<br>
&gt;         &gt; _______________________________________________<br>
&gt;         &gt; Bio-Linux mailing list<br>
&gt;         &gt; <a href="mailto:Bio-Linux@nebclists.nerc.ac.uk">Bio-Linux@nebclists.nerc.ac.uk</a><br>
&gt;         &gt; <a href="http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux" rel="noreferrer" target="_blank">http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux</a><br>
&gt;<br>
&gt;         --<br>
&gt;         Tim Booth &lt;<a href="mailto:tbooth@ceh.ac.uk">tbooth@ceh.ac.uk</a>&gt;<br>
&gt;<br>
&gt;         Centre for Ecology and Hydrology<br>
&gt;         Maclean Bldg, Benson Lane<br>
&gt;         Crowmarsh Gifford<br>
&gt;         Wallingford, England<br>
&gt;         OX10 8BB<br>
&gt;<br>
&gt;         <a href="http://environmentalomics.org/bio-linux" rel="noreferrer" target="_blank">http://environmentalomics.org/bio-linux</a><br>
&gt;         <a href="tel:%2B44%201491%2069%202297" value="+441491692297">+44 1491 69 2297</a><br>
&gt;<br>
&gt;<br>
&gt;         _______________________________________________<br>
&gt;         Bio-Linux mailing list<br>
&gt;         <a href="mailto:Bio-Linux@nebclists.nerc.ac.uk">Bio-Linux@nebclists.nerc.ac.uk</a><br>
&gt;         <a href="http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux" rel="noreferrer" target="_blank">http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux</a><br>
&gt;<br>
&gt;<br>
&gt; _______________________________________________<br>
&gt; Bio-Linux mailing list<br>
&gt; <a href="mailto:Bio-Linux@nebclists.nerc.ac.uk">Bio-Linux@nebclists.nerc.ac.uk</a><br>
&gt; <a href="http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux" rel="noreferrer" target="_blank">http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux</a><br>
<br>
--<br>
Tim Booth &lt;<a href="mailto:tbooth@ceh.ac.uk">tbooth@ceh.ac.uk</a>&gt;<br>
<br>
Centre for Ecology and Hydrology<br>
Maclean Bldg, Benson Lane<br>
Crowmarsh Gifford<br>
Wallingford, England<br>
OX10 8BB<br>
<br>
<a href="http://environmentalomics.org/bio-linux" rel="noreferrer" target="_blank">http://environmentalomics.org/bio-linux</a><br>
<a href="tel:%2B44%201491%2069%202297" value="+441491692297">+44 1491 69 2297</a><br>
<br>
<br>
_______________________________________________<br>
Bio-Linux mailing list<br>
<a href="mailto:Bio-Linux@nebclists.nerc.ac.uk">Bio-Linux@nebclists.nerc.ac.uk</a><br>
<a href="http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux" rel="noreferrer" target="_blank">http://nebclists.nerc.ac.uk/mailman/listinfo/bio-linux</a><br>
</blockquote></div><br></div>