Hi, Glen I did as u said but I still face the same error . Can u see and advise me on what might have gone wrong? Rgs,Sharon root at frontend-0 share]# dbreport machines compute-0-0 [root at frontend-0 share]# /usr/local/mpich-vmi2/bin/mpirun -np 25 /etc/machin/opt/BioBrew/NCBI/6.1.0/bin/mpiblast --config-file=/opt/BioBrew/NCBI/6.1.0/etc/mpiblast.conf -p blastn -d /usr/local/share/db/ecoli.nt -i /root/test.txt -o ./blast_results.txt Warning: Command line arguments for program should be given after the program name. Assuming that --config-file=/opt/BioBrew/NCBI/6.1.0/etc/mpiblast.conf is a command line argument for the program. Warning: Command line arguments for program should be given after the program name. Assuming that -p is a command line argument for the program. Warning: Command line arguments for program should be given after the program name. Assuming that blastn is a command line argument for the program. Warning: Command line arguments for program should be given after the program name. Assuming that -d is a command line argument for the program. Warning: Command line arguments for program should be given after the program name. Assuming that /usr/local/share/db/ecoli.nt is a command line argument for the program. Warning: Command line arguments for program should be given after the program name. Assuming that -i is a command line argument for the program. Unrecognized argument /etc/machin/opt/BioBrew/NCBI/6.1.0/bin/mpiblast ignored. Error: No machinefile specified. You must specify a valid machinefile or populate the default machinefile: /usr/local/mpich-vmi2/bin/../share/machines.list >From: Glen Otero <gotero at linuxprophet.com> >Reply-To: BioBrew-Users at bioinformatics.org >To: BioBrew-Users at bioinformatics.org >Subject: [BioBrew Users] Fwd: Do anyone know how to resolve this error? >Date: Tue, 9 Mar 2004 00:08:19 -0800 > > > >Begin forwarded message: > >>From: Glen Otero <gotero at linuxprophet.com> >>Date: March 9, 2004 12:03:32 AM PST >>To: "shalini sharon" <proclaim_jesus at hotmail.com> >>Subject: Re: Do anyone know how to resolve this error? >> >>You need to populate the default machine file (machines.list) with the >>list of hostnames of the machines that you would like to run mpi jobs on. >>For example: >> >># Default machine list for mpirun.ch_vmi. Newline delimited. >>compute-0-0 >>compute-0-1 >>... >>compute-0-(N-1) >> >>Running this command: >> >>dbreport machines > /usr/local/mpich-vmi2/share/machines.list >> >>will populate the machines.list with all of your compute nodes. >> >>Glen >> >>On Mar 8, 2004, at 11:53 PM, shalini sharon wrote: >> >>> >>>Hi, Sharon here.. >>> >>> This is the ERROR I faced. >>> >>> Error: No machilefile specified >>> You must specify a valid machinefile or populate the defauly >>>machinefile >>> /usr/local/mpich-vmi2/bin/../share/machines.list >>> >>>_________________________________________________________________ >>>Find gifts, buy online with MSN Shopping. http://shopping.msn.com.sg/ >>> >>> >>> >>Glen Otero, Ph.D. >>Linux Prophet >>619.917.1772 >> >> >Glen Otero, Ph.D. >Linux Prophet >619.917.1772 > _________________________________________________________________ Get 10mb of inbox space with MSN Hotmail Extra Storage http://join.msn.com/?pgmarket=en-sg