hi I'm using the seqsplit & blastunsplit ftp://ftp.cgr.ki.se/pub/prog/MSPcrunch+Blixem/seqsplit.tar.Z my work is 1. $seqsplit -c 12000 test.seq 2. $ls test.0 test.1 test.2.test.3 test.4 test.5 3. blastwork $ blastall -a2 -d nr -p blastx -i test.0(1,2,3,4,5) -o test.0(1,2,3,4,5).bl 4. after I run the blastunslplit $ blastunsplit test.seq Reconstructing header ... Chunk 0: 12465 residues [Total 12465] Chunk 1: 12459 residues [Total 24924] Chunk 2: 12456 residues [Total 37380] Chunk 3: 12453 residues [Total 49833] Chunk 4: 12445 residues [Total 62278] Chunk 5: 1759 residues [Total 64037] Total length of query with overlaps (from .bl files): 64037 FATAL ERROR: Wrong chunk and/or overlap sizes specified! They give a total length of 254259, whereas the .bl files give 64037 --- or ----- $blastunsplit -c 12000 test.seq Reconstructing header ... Chunk 0: 12465 residues [Total 12465] Chunk 1: 12459 residues [Total 24924] Chunk 2: 12456 residues [Total 37380] Chunk 3: 12453 residues [Total 49833] Chunk 4: 12445 residues [Total 62278] Chunk 5: 1759 residues [Total 64037] Total length of query with overlaps (from .bl files): 64037 FATAL ERROR: Wrong chunk and/or overlap sizes specified! They give a total length of 64259, whereas the .bl files give 64037 5. how can I do