Chunlin, Are you getting the Bailing out with signal 2 [3] MPI Abort by user Aborting program ! p3_30777: p4_error: : 0 or [blastall] ERROR: Threshold for extending hits, default if zero blastp 11, blastn 0, blastx 12, tblastn 13 tblastx 13, megablast 0 [/usr/local/mpiblast.conf] is bad or out of range [? to ?] If the former, could you send the output of mpiblast run with --debug? If the latter, from ncbi/build/blastall --help: -f Threshold for extending hits, default if zero blastp 11, blastn 0, blastx 12, tblastn 13 tblastx 13, megablast 0 [Integer] default = 0 you have to specify mpiblast --config-file=</path/mpiblast.conf> , -f is for passing options to BLAST itself. This is a bit confusing, as mpiformatdb takes -f or --config-file for the config file. -Lucas On Thu, Jul 24, 2003 at 11:40:42AM -0500, Chunlin Wang wrote: > > Jeremy, > > > I run mpiBlast in a Linux cluster and got pretty the same error > message you pasted on 23 May. Would you please send me some helpful > information how to solve that problem? > > > Thanks ahead, > > > Chunlin Wang