> We downloaded a standalone blast web server from NCBI website last year. > It has been working fine until very recently. A few new datasets with > long names > were added into the directory where we have all the blastable datasets. > After that, blastn > works for some datasets and all other types of blast search don't work. > If I remove these long-name > datasets, everything would work. There are no strange characters in > these names except > a few have '+' sign in the name. The problem doesn't go away if I only > remove the ones with '+' sign. > These long-name datasets themselves work ok for blastn search. The > problem is that their existence interfere > with normal function of other datasets. > If you know the cause of this problem. Please help, many thanks. > Yu-yu If your using the preformatted datasets, then you'll need to update to the 2.2.5 of the BLAST binaries since they are introducing a slightly new format to the databases. I think this is what your experiencing. -- Jeremy Mann jeremy@biochem.uthscsa.edu University of Texas Health Science Center Bioinformatics Core Facility http://www.bioinformatics.uthscsa.edu Phone: (210) 567-2672