This is a multi-part message in MIME format. ------=_NextPart_000_0008_01C31A38.CD81EA40 Content-Type: text/plain; charset="big5" Content-Transfer-Encoding: quoted-printable thx~John~ i mpiformat nr again and the message as follow: >Breaking up /home/ct/toolkit/mpiBLAST-1.0.1/nr (458.894995 MB) into 8 = >fragments of 57.361874375 MB >/home/ct/toolkit/ncbi/bin/formatdb -i nr -v 57.361874375 -p T -o T: = exit status: 0 >Moving fragments to /home/ct/toolkit/mpiBLAST-1.0.1 >ls: /home/ct/toolkit/mpiBLAST-1.0.1/nr.*.nsq: No such file or directory >You must create at least two fragments to use mpiblast. so i guess that there is some problem in the file "mpiblast.conf" i set "mpiblast.conf" as follow >/home/ct/toolkit/mpiBLAST-1.0.1 >/home/ct/toolkit/mpiBLAST-1.0.1 >/home/ct/toolkit/ncbi/bin in fact=20 i believe i didn't installed mpiBLAST correctly because when i use "mpiblast blastn" to search "yeast.nt" every time there are some error message like this >cp: `/home/ct/toolkit/mpiBLAST-1.0.1/src/yeast.nt.00.nhr' and = `/home/ct/toolkit/mpiBLAST-1.0.1/src/yeast.nt.00.nhr' are the same file >cp: `/home/ct/toolkit/mpiBLAST-1.0.1/src/yeast.nt.00.nin' and = `/home/ct/toolkit/mpiBLAST-1.0.1/src/yeast.nt.00.nin' are the same file >.. >.. >cp: `/home/ct/toolkit/mpiBLAST-1.0.1/src/yeast.nt.07.nsi' and = `/home/ct/toolkit/mpiBLAST-1.0.1/src/yeast.nt.07.nsi' are the same file >[blastall] WARNING: test2: Could not find taxdb.bti but i can get the search result anybody could solve these problems? thanks a lot~: ) -Lee ------=_NextPart_000_0008_01C31A38.CD81EA40 Content-Type: text/html; charset="big5" Content-Transfer-Encoding: quoted-printable <!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN"> <HTML><HEAD> <META http-equiv=3DContent-Type content=3D"text/html; charset=3Dbig5"> <META content=3D"MSHTML 6.00.2600.0" name=3DGENERATOR> <STYLE></STYLE> </HEAD> <BODY bgColor=3D#ffffff> <DIV>thx~John~</DIV> <DIV>i mpiformat nr again</DIV> <DIV>and the message as follow:</DIV> <DIV>>Breaking up /home/ct/toolkit/mpiBLAST-1.0.1/nr (458.894995 MB) = into 8=20 >fragments of 57.361874375 = MB<BR>>/home/ct/toolkit/ncbi/bin/formatdb =20 -i nr -v 57.361874375 -p T -o T: exit status: 0<BR>>Moving fragments = to=20 /home/ct/toolkit/mpiBLAST-1.0.1<BR>>ls:=20 /home/ct/toolkit/mpiBLAST-1.0.1/nr.*.nsq: No such file or = directory<BR>>You=20 must create at least two fragments to use mpiblast.<BR>so i guess that = there is=20 some problem in the file "mpiblast.conf"</DIV> <DIV>i set "mpiblast.conf" as follow</DIV> <DIV>>/home/ct/toolkit/mpiBLAST-1.0.1<BR>>/home/ct/toolkit/mpiBLAST= -1.0.1<BR>>/home/ct/toolkit/ncbi/bin<BR>in=20 fact </DIV> <DIV>i believe i didn't installed mpiBLAST correctly</DIV> <DIV>because when i use "mpiblast blastn" to search "yeast.nt" every = time</DIV> <DIV>there are some error message like this</DIV> <DIV>>cp: `/home/ct/toolkit/mpiBLAST-1.0.1/src/yeast.nt.00.nhr' and=20 `/home/ct/toolkit/mpiBLAST-1.0.1/src/yeast.nt.00.nhr' are the same=20 file<BR>>cp: `/home/ct/toolkit/mpiBLAST-1.0.1/src/yeast.nt.00.nin' = and=20 `/home/ct/toolkit/mpiBLAST-1.0.1/src/yeast.nt.00.nin' are the same = file</DIV> <DIV>>..</DIV> <DIV>>..</DIV> <DIV>>cp: `/home/ct/toolkit/mpiBLAST-1.0.1/src/yeast.nt.07.nsi' and=20 `/home/ct/toolkit/mpiBLAST-1.0.1/src/yeast.nt.07.nsi' are the same=20 file<BR>>[blastall] WARNING: test2: Could not find taxdb.bti</DIV> <DIV>but i can get the search result</DIV> <DIV><SPAN class=3D758550920-13052003>anybody could solve these=20 problems?</SPAN></DIV> <DIV><FONT size=3D2>thanks a lot~: )</FONT></DIV> <DIV> </DIV> <DIV><FONT size=3D2>-Lee</FONT></DIV> <DIV> </DIV> <DIV> </DIV> <DIV> </DIV> <DIV> </DIV></BODY></HTML> ------=_NextPart_000_0008_01C31A38.CD81EA40--