This message is in MIME format. Since your mail reader does not understand this format, some or all of this message may not be legible. ------_=_NextPart_001_01C31A1B.BC5DAA00 Content-Type: text/plain; charset="big5" > i set "mpiblast.conf" as follow >/home/ct/toolkit/mpiBLAST-1.0.1 >/home/ct/toolkit/mpiBLAST-1.0.1 >/home/ct/toolkit/ncbi/bin [Osborne, John] This isn't correct, you need to set up a shared storage location (on an NFS mount or whatever) and a local storage location. You don't want to have your ncbi directory in there at all. Mine looks something like this: /scratch/storage/shared /scratch/storage/mpiblast_local According to the readme, this file should contain 3 lines. I think this is either a typo or the last line is a blank line (I put a blank line in mine). Your .ncbirc file should have the location of your blast data directory. Hope this helps. I am going to devote the rest of my morning to getting mpiblast to run properly, so feel free to email this list. -John ------_=_NextPart_001_01C31A1B.BC5DAA00 Content-Type: text/html; charset="big5" <!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN"> <HTML><HEAD> <META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=big5"> <META content="MSHTML 6.00.2800.1141" name=GENERATOR> <STYLE></STYLE> </HEAD> <BODY bgColor=#ffffff> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2>> </FONT></SPAN>i set "mpiblast.conf" as follow<BR>>/home/ct/toolkit/mpiBLAST-1.0.1<BR>>/home/ct/toolkit/mpiBLAST-1.0.1<BR>>/home/ct/toolkit/ncbi/bin<BR><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2>[Osborne, John] </FONT></SPAN></DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2>This isn't correct, you need to set up a shared storage location (on an NFS mount or </FONT></SPAN><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2>whatever) and a local</FONT></SPAN></DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2> storage location. You don't want to have your ncbi directory in there at all. Mine looks </FONT></SPAN><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2>something like this:</FONT></SPAN></DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2>/scratch/storage/shared</FONT></SPAN></DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2>/scratch/storage/mpiblast_local</FONT></SPAN></DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2></FONT></SPAN> </DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2>According to the readme, this file should contain 3 lines. I think this is either a typo or the last line</FONT></SPAN></DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2>is a blank line (I put a blank line in mine).</FONT></SPAN></DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2></FONT></SPAN> </DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2>Your .ncbirc file should have the location of your blast data directory.</FONT></SPAN></DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2></FONT></SPAN> </DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2>Hope this helps. I am going to devote the rest of my morning to getting mpiblast to run properly, so feel free</FONT></SPAN></DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2>to email this list.</FONT></SPAN></DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2></FONT></SPAN> </DIV> <DIV><SPAN class=542491413-14052003> <FONT face=Arial color=#0000ff size=2> -John</FONT></SPAN></DIV> <DIV><SPAN class=542491413-14052003><FONT face=Arial color=#0000ff size=2></FONT></SPAN> </DIV></BODY></HTML> ------_=_NextPart_001_01C31A1B.BC5DAA00--