> Richard Huang wrote: > > Hi, > > I'm trying to run mpiBLAST on the nr peptide database using the first > entry as the query. I've installed the NCBI toolbox and also MPI. I > get the following error when I try to run: > > [ryhuang@frontend-0 mpiBLAST-1.2.0]$ mpirun -np 5 -nolocal > -machinefile machines /home/ryhuang/mpiBLAST-1.2.0/bin/mpiblast > --debug --log-file=/home/ryhuang/mpiBLAST-1.2.0/log3 -p blastp -d > nr_092602.fa -i query2.fas -o result.txt > logging to /home/ryhuang/mpiBLAST-1.2.0/log3.1 > logging to /home/ryhuang/mpiBLAST-1.2.0/log3.2 > logging to logging to /home/ryhuang/mpiBLAST-1.2.0/log3.4 > /home/ryhuang/mpiBLAST-1.2.0/log3.0 > logging to /home/ryhuang/mpiBLAST-1.2.0/log3.3 > [blastall] ERROR: ncbiapi [000.000] ObjMgrChoice: pointer [0] type > [1] not found <snip> This is a bug in mpiBLAST 1.2.0. Until it is fixed, you should format the database with "-o T" (i.e. Parse SeqId and create indexes) as a work around. Regards, Jason