mpiBLAST is ready to run in BioBrew. 1)I had set up .ncbirc [NCBI] Data=/opt/BioBrew/NCBI/6.1.0/data [BLAST] BLASTDB=/tmp/blast 2) and mpiblast.conf /mnt/pvfs /tmp/blast /opt/BioBrew/NCBI/6.1.0/bin 3) edit my /etc/profile export BLASTPATH=/opt/BioBrew/NCBI/6.1.0/bin export MPIBLASTCONFIG=/home/bigcpuguest/mpiblast.conf export MPIRUN=/usr/bin/mpirun 4) my $HOME/machines contains comp-pvfs-0-0 Can anyone tell me what is the correct command if I want to run mpiblast against ecoli.nt? _________________________________________________________________ Get 10mb of inbox space with MSN Hotmail Extra Storage http://join.msn.com/?pgmarket=en-sg