Hi all! Iam running mpiblast with the following command. mpirun -np 4 ~/bin/mpiblast -p blastp -d pir.fasta -i /home/kalyani/hmmer-2.3.2/src/seq1 -o mpiblastoutput.txt Iam getting the following error. Can any one of you help me in finding out the error. Iam attaching the .ncbirc file Thanks in Advance Kalyani [blastall] ERROR: seq31: BlastKarlinBlkGappedCalc: PAM30 is a supported matrix [blastall] ERROR: seq31: BlastKarlinBlkGappedCalc: PAM70 is a supported matrix [blastall] ERROR: seq32: BlastKarlinBlkGappedCalc: èÑ is not a supported matrix [blastall] ERROR: seq32: BlastKarlinBlkGappedCalc: BLOSUM80 is a supported matrix [blastall] ERROR: seq32: BlastKarlinBlkGappedCalc: BLOSUM62 is a supported matrix [blastall] ERROR: seq32: BlastKarlinBlkGappedCalc: BLOSUM50 is a supported matrix [blastall] ERROR: seq32: BlastKarlinBlkGappedCalc: BLOSUM45 is a supported matrix [blastall] ERROR: seq32: BlastKarlinBlkGappedCalc: PAM250 is a supported matrix [blastall] ERROR: seq32: BlastKarlinBlkGappedCalc: BLOSUM62_20 is a supported matrix [blastall] ERROR: seq32: BlastKarlinBlkGappedCalc: BLOSUM90 is a supported matrix [blastall] ERROR: seq32: BlastKarlinBlkGappedCalc: PAM30 is a supported matrix [blastall] ERROR: seq32: BlastKarlinBlkGappedCalc: PAM70 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: â is not a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: BLOSUM80 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: BLOSUM62 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: BLOSUM50 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: BLOSUM45 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: PAM250 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: BLOSUM62_20 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: BLOSUM90 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: PAM30 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: PAM70 is a supported matrix 2 0.0277332 Bailing out with signal 11 MPI: Program /home/kalyani/bin/mpiblast, Rank 2, Process 9189 called MPI_Abort(<communicator>, 0) MPI: --------stack traceback------- [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: â is not a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: BLOSUM80 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: BLOSUM62 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: BLOSUM50 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: BLOSUM45 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: PAM250 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: BLOSUM62_20 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: BLOSUM90 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: PAM30 is a supported matrix [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: PAM70 is a supported matrix 3 0.0337366 Bailing out with signal 11 ########### .nbirc file contains [NCBI] Data=/home/kalyani/toolkit/ncbi/data [BLAST] BLASTDB=/home/kalyani/toolkit/ncbi BLASTMAT=/home/kalyani/toolkit/ncbi/data [mpiBLAST] Shared=/home/kalyani/toolkit/ncbi Local=/home/kalyani/toolkit/ncbi