Thanks for joining this discussion list. As the introductory message implies, this group is meant to be an open forum for discussing all topics relating to development issues for those engaged in bioinformatics and related efforts. I personally use a very broad definition of bioinformatics, as an all-encompassing study of the information content of living systems, and how the life processes make use of the information. This means in my mind that we are looking at classical genomics (primary structure), computational chemistry (secondary structure, SAR, etc), computational biophysics, and many other topics. Even if this definition of bioinformatics doesnt necessarily match with yours, please feel free to contribute your thoughts and comments. In order to try to jumpstart a discussion, I would like to talk briefly about some of the work I am currently doing. My work as of late has been in designing and building scalable computing systems (software and hardware). I have been using SOAP and XML as well as object oriented Perl. Some of the issues I am running into on the development side involve creating persistent objects to temporarily store complex data structures on my server. Basically the type of thing that I would like to do is to take either a hash or an XML document such as follows: <?xml version="1.0"?> <compute_server> <server> <host>localhost</host> <port>6931</port> <listen>10</listen> <protocol>http</protocol> </server> </compute_server> <database> <host>bio0</host> <name>jobdb</name> <table>rundb</table> </database> </config> and have this persistent across calls. There are several "easy" ways to do this. One method is to "serialize" this data structure, and to store the serialized verision. Many "XML databases" do exactly that. What would be nice is to have a simple set of (Perl or Perl callable) methods to trivially store and retrieve this structure or elements of this structure. I have written a wrapper above the Perl DBI to make it very OO looking/acting, and maybe this is the route I need to take. If anyone has done anything related to this, I would like to hear about it. As indicated, I am using SOAP, and the SOAP::Lite module in Perl. This makes building "web visible" services quite simple. Of course, nothing is without cost. What I have found is that after calling a SOAP new method to instantiate the object, I cannot use the traditional Perl OO methods to decorate the object with attributes, that is mutators and accessors: # # mutator # sub set_thingy { my ($self,$thingy) = @_; return $self->{'thingy'} = $thingy ; } # # accessor # sub get_thingy { my ($self) = @_; if (exists($self->{'thingy'})) { return $self->{'thingy'}; } else { return undef; } } does not work anymore (works great outside of SOAP::Lite). This is a little bothersome, as I use these types of accessors/mutators all the time. Hence my need for a persistent object storage on the remote host. On an unrelated note, I am curious as to what analysis techniques people are using for proteomic data. At this stage, I have seen image processing work, various bits of data mining and regression tests, etc. It of course is highly dependent upon the processes being studied. My interests are specifically in those analyses which are computationally demanding. Thanks, and once again, welcome to the group!