You might look into SeqHound which is built on top of the NCBI toolbok. http://seqhound.mshri.on.ca/apihelp.html and may be easier than trying to talk to NCBI directly and provides what may be a more intuitive API. Alternatively if you are willing to download the data several toolkits {BioPerl,BioJava,BioPython, several C libraries} exist for building local indexes to sequence data. It depends on what type of information you are trying to extract. -jason On Wed, 17 Sep 2003, Singhal, Mudita wrote: > Hello Everyone, > > Does anyone know of any programs/api using which we can query the NCBI > database directly from our application instead of going through each > individual query from the web interface?To explain more, why we need > such a program is that when we have a lot of repeated hits from one port > number to the NCBI web interface it blocks that port number. > > Thanks > Mudita > _______________________________________________ > Biodevelopers mailing list > Biodevelopers at bioinformatics.org > https://bioinformatics.org/mailman/listinfo/biodevelopers > -- Jason Stajich Duke University jason at cgt.mc.duke.edu