[Biodevelopers] Subcellular localization?

Ethan Strauss ethan.strauss at promega.com
Wed Nov 23 15:21:40 EST 2005


Hi, 
   I have a list of about 2500 accession numbers from Genbank Refseq.
All of them are human coding sequences and I can easily get the complete
sequence and other information from Genbank, but I can't figure out a
way to get subcellular localization information. I have pulled some data
from UniProt and from DBSubLoc
(http://www.bioinfo.tsinghua.edu.cn/dbsubloc.html) and have been able to
match about 10% of my sequences to subcellular localizations from these
databases, but that still leaves about 90% unknown. One problem is that
I can't find a way to match Genbank Accession # with the IDs in
Swiss-Prot and DBSubLoc. I have just gone on sequence identity (So far I
only call it a match when it is 100% identical). 
Do you have any ideas about how I can get subcellular localization info
for the rest of my sequences? 
Thanks for any help or suggestions!
Ethan

Ethan Strauss Ph.D.
Bioinformatics Scientist
Promega Corporation
2800 Woods Hollow Rd.
Madison, WI 53711
608-274-4330
800-356-9526
ethan.strauss at promega.com




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