Hi, i'm wondering if it's possible to get all sequences of a large database ranked by E-value or score from PSI-BLAST with a query. Normally PSI-BLAST stops outputting after a couple of sequences even if one sets the output parameters -b or -E to very high values. Is it possible in general or are there computational limits (time etc.) in figuring out the right scores or E-values when it comes to the many sequences with very low identity? Thanks for any advice. Martin -- + Neural Processing Group Technical University Berlin + http://ni.cs.tu-berlin.de + Institute of Bioinformatics Johannes Kepler University Linz + http://www.bioinf.jku.at/ + In the beginning was the WORD, and the WORD was UNSIGNED, + and the main(){} was without form and void