Dr. Blake, This question was asked before (in our lab). Could you provide an answer? Thanks. Jeff > I was wondering if the Meltism program can account for melting > differences due to meCpG status. Methylation of C residues greatly > increases the melting temperature of DNAs (Ehrlich,M et al., 1975, > Biochim. biophys. Acta, 395:109-119; Collins,M. & Myers,RM 1987, J. Mol. > Biol. 198:737-44). If Meltism does not permit for this variation can it > be modified? Are there other programs that may be able to perform such > an analyses? Thank you in advance for your assistance on this. -- J.W. Bizzaro jeff at bioinformatics.org Director, Bioinformatics.org: The Open Lab http://bioinformatics.org/~jeff "All those scientists--they're all alike! They say they're working for us, but what they really want is to rule the world!" -- Angry Villager, Young Frankenstein --