Carla Clemente wrote: > > If I open the test sequence file using the Melt program and press begin in > melt menu, appears an application error "......'0,075' is not a valid > floating point value" even if I try to change this value. Are you trying to open the file that I sent with Melt? As I mentioned, the file I sent is annotated. It needs to be Compiled first into raw DNA sequences. As another test, you can just create a text file filled with "ACGTAGCAGCACGTATTAGACGAT", and open with Melt. Hmmmm, but seeing that '0,075' value, I wonder if you're using non-English decimal notation. You should try entering 0.075. Cheers. Jeff -- J.W. Bizzaro jeff at bioinformatics.org Director, Bioinformatics.Org http://bioinformatics.org/~jeff "As we enjoy great advantages from the inventions of others, we should be glad of an opportunity to serve others by any invention of ours; and this we should do freely and generously." -- Benjamin Franklin --