Hmm. 0.075 (or 0,075) appears to be the salt concentration. Are you typing 0.075, 0,075 or 0? Jeff Carla Clemente wrote: > > I also tried to create a text file filled with your DNA sequence (.seq) and > open with Melt. But when I press begin, occurs the same error and I´m using > English decimal notation. I tried to download the program again but didn´t > work out. > > Another question is, the DNA buffer doesn´t have salt, so I have to write 0 > in salt concentration box. Is there any problem?