[meltsim] Re: [Fwd: [meltsim] Information]

J.W. Bizzaro jeff at bioinformatics.org
Thu Dec 13 11:51:18 EST 2001

Carla Clemente wrote:
> I already found the error. I was typing 0.075, but my computer had
> non-English decimal notation. So I changed it and the Melt program ran.

Great.  I was thinking it was something like that.

> But I had another problem, because the DNA buffer doesn´t have salt, so I
> tried to type 0.00 and the error appears.
Which error, "0,075 is not a valid floating point"?

> If I decrease the salt
> concentration (0.075, 0.06, 0.03......0.001),  the Tm decrease from 87.5ºC
> to 65 ºC. Is this normal?

Absolutely.  Tm is directly proportional to salt concentration.


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