Carla Clemente wrote: > > I already found the error. I was typing 0.075, but my computer had > non-English decimal notation. So I changed it and the Melt program ran. Great. I was thinking it was something like that. > But I had another problem, because the DNA buffer doesn´t have salt, so I > tried to type 0.00 and the error appears. ^^^ Which error, "0,075 is not a valid floating point"? > If I decrease the salt > concentration (0.075, 0.06, 0.03......0.001), the Tm decrease from 87.5ºC > to 65 ºC. Is this normal? Absolutely. Tm is directly proportional to salt concentration. Jeff