Carla Clemente wrote: > > I know that Tm is directly proportional to salt concentration, but which is > the constant of proporcionality? It seems to be too higher. Do you mean Tm is decreasing more than you expect? > And when I tried to typing 0.00 salt concentration appears "floating point > division by zero" Try a very small number, like 1e-100. Jeff -- J.W. Bizzaro jeff at bioinformatics.org Director, Bioinformatics.org http://bioinformatics.org/~jeff "As we enjoy great advantages from the inventions of others, we should be glad of an opportunity to serve others by any invention of ours; and this we should do freely and generously." -- Benjamin Franklin --