[meltsim] [Fwd: [Fwd: [meltsim] Information]]

Richard.Blake rdblake at attglobal.net
Thu Dec 20 11:24:51 EST 2001


Jeff, you've responded very well (and with extraordinary
patience). Regarding her last question, the dependence
of Tm on ionic strength varies with the (G+C)-content:
a larger dependence for (A+T)-rich DNAs than for
(G+C)-rich DNAs. Rule of thumb is a change of
about 20 degrees per decade change in salt for the former,
and only ~14 degrees for the latter. What sort of change is she
finding, or doesn't she know?
dick.
ps. how is your progress?
 
At 11:44 AM 12/17/01 -0500, you wrote:
>Dr. Blake, any thoughts?  Jeff
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>Date: Mon, 17 Dec 2001 16:42:34 +0000
>From: Carla Clemente <carlac at itqb.unl.pt>
>Subject: Re: [Fwd: [meltsim] Information]
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>
>>Do you mean Tm is decreasing more than you expect?
>>
>>Yes! What do you think about that?
>
>
>
>Carla
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>



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