[meltsim] [Fwd: [Fwd: [meltsim] Information]]

[Richard.Blake]

Jeff, you've responded very well (and with extraordinary
patience). Regarding her last question, the dependence
of Tm on ionic strength varies with the (G+C)-content:
a larger dependence for (A+T)-rich DNAs than for
(G+C)-rich DNAs. Rule of thumb is a change of
about 20 degrees per decade change in salt for the former,
and only ~14 degrees for the latter. What sort of change is she
finding, or doesn't she know?
dick.
ps. how is your progress?
 
At 11:44 AM 12/17/01 -0500, you wrote:
>Dr. Blake, any thoughts?  Jeff
>Return-path: <carlac at itqb.unl.pt>
>Received: from gatekeeper.itqb.unl.pt
> (qmailr at gatekeeper.itqb.unl.pt [193.136.177.1])	by www.bioinformatics.org
> (8.9.3/8.8.7) with SMTP id LAA09748	for <jeff at bioinformatics.org>; Mon,
> 17 Dec 2001 11:40:05 -0500
>Received: (qmail 11521 invoked from network); 17 Dec 2001 16:40:38 -0000
>Received: from mistral.itqb.unl.pt (root at 193.136.176.14)
>  by gatekeeper.itqb.unl.pt with SMTP; 17 Dec 2001 16:40:38 -0000
>Received: from orfeu1.itqb.unl.pt (orfeu1.itqb.unl.pt [193.136.181.128])
>	by mistral.itqb.unl.pt (8.9.3/8.9.3) with ESMTP id QAA29481	for
> <jeff at bioinformatics.org>; Mon, 17 Dec 2001 16:40:37 GMT
>Date: Mon, 17 Dec 2001 16:42:34 +0000
>From: Carla Clemente <carlac at itqb.unl.pt>
>Subject: Re: [Fwd: [meltsim] Information]
>In-reply-to: <3C1DFF51.86759994 at bioinformatics.org>
>X-Sender: carlac at mailer.itqb.unl.pt
>To: "J.W. Bizzaro" <jeff at bioinformatics.org>
>Message-id: <5.1.0.14.0.20011217164111.009ed8b0 at mailer.itqb.unl.pt>
>MIME-version: 1.0
>X-Mailer: QUALCOMM Windows Eudora Version 5.1
>Content-type: text/plain; format=flowed; charset=us-ascii
>References: <5.1.0.14.0.20011205093552.009efe80 at mailer.itqb.unl.pt>
> <5.1.0.14.0.20011205162255.009f24b0 at mailer.itqb.unl.pt>
> <5.1.0.14.0.20011207200819.009f0d20 at mailer.itqb.unl.pt>
> <5.1.0.14.0.20011212181802.009faea0 at mailer.itqb.unl.pt>
> <5.1.0.14.0.20011217110419.009f0d30 at mailer.itqb.unl.pt>
>
>
>>Do you mean Tm is decreasing more than you expect?
>>
>>Yes! What do you think about that?
>
>
>
>Carla
>
>
>