Joseph Landman wrote > Hmm. Does it impact the output data you need (actual alignment > information), or are you after parameters and computational "side > effects" (bit scores, E-values,...) Careful of your assumptions here! For many of us the E-values and IDs arethe only useful data out of BLAST, with the alignments being so poor as to be discarded. This is a common enough usage of blast that there is even an output format for it (-m 9 is the format I use). ------------------------------------------------------------ Kevin Karplus karplus at soe.ucsc.edu http://www.soe.ucsc.edu/~karplus Senior member, IEEE Board of Directors, ISCB (starting Jan 2005) Professor of Biomolecular Engineering, University of California, Santa Cruz Undergraduate and Graduate Director, Bioinformatics Affiliations for identification only.