If your intention is to do this using hmmalign (as the subject of your message implies), take your query sequence and a member of the seed alignment used to make the HMM and put them into fasta_file. Then do: hmmalign -o output_file HMM fasta_file Of course, you can take as many sequences from the seed alignment as you want. This doesn't mean that the alignment will be optimal - it will only be optimal according to the HMM used. Hope this helps, Mensur At 02:21 PM 5/19/2005, you wrote: >Dear All, > >Is there a way I can get individual alignments for a query sequence with the >members of the HMM profile? > >Thanks in advance. > >Best > >Rajesh ========================================================================== | Mensur Dlakic, PhD | Tel: (406) 994-6576 | | Department of Microbiology | Fax: (406) 994-4926 | | Montana State University | | | 109 Lewis Hall, P.O. Box 173520 | http://myprofile.cos.com/mensur | | Bozeman, MT 59717-3520 | E-mail: mdlakic at montana.edu | ==========================================================================