PrimerPy is a GUI tool for optimal SG QPCR primer design. This branch, Huckleberry, uses "Primer3" as backend engine. The design's been validated by laboratory experiences; also applicable to conventional PCR.
PrimerPy is open source and free under Python license. We welcome user input and code contributions.
PrimerPy 0.93a (tortilla) is released. Please visit
for the newest and downloads.