:: PredictBias ::

A) Input parameters
Genome	G27.gbk	Threshold dinucleotide bias	2
Threshold codon bias	4	Threshold %GC bias	3
E-value (RPSBlast)	0.05	Genome (non-pathogenic)

B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start end
Select Organism

C) Potential GIs and PAIs in CP001173 (download)

S.No

Start

End

Bias

Virulence

Insertion elements

Prediction

HPG27_43

HPG27_81

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_43	-2	15	-3.299152	hypothetical protein
HPG27_44	-2	11	-2.330826	adenine specific DNA methyltransferase (putative
HPG27_45	0	9	-1.129168	cytosine specific DNA methyltransferase
HPG27_46	0	10	-1.371410	hypothetical protein
HPG27_47	0	11	-1.176106	hypothetical protein
HPG27_48	1	11	-0.965423	hypothetical protein
HPG27_50	1	11	0.333935	sodium/proline symporter
HPG27_51	3	15	-0.371012	proline/delta1-pyrroline-5-carboxylate
HPG27_52	7	22	-2.089432	hypothetical protein
HPG27_53	8	20	-2.695980	hypothetical protein
HPG27_54	5	17	-0.659899	hypothetical protein
HPG27_56	4	16	0.994937	hypothetical protein
HPG27_57	4	16	1.227648	hypothetical protein
HPG27_59	3	13	1.769593	hypothetical protein
HPG27_60	6	21	2.978807	hypothetical protein
HPG27_62	6	23	3.507738	urease accessory protein
HPG27_63	4	23	2.948433	urease accessory protein
HPG27_65	3	17	2.606258	urease accessory protein
HPG27_66	3	19	2.598793	urease accessory protein / pH-dependent
HPG27_67	1	17	2.492862	urease B
HPG27_68	-2	10	1.895725	urease A
HPG27_69	1	14	1.966449	*lipoprotein signal peptidase
HPG27_70	1	14	2.373822	phosphoglucosamine mutase
HPG27_71	2	15	2.310049	ribosomal protein S20
HPG27_72	2	15	2.309333	peptide chain release factor RF-1
HPG27_73	2	16	1.923830	outer membrane protein
HPG27_74	0	15	1.309323	hypothetical protein
HPG27_76	0	14	0.357941	ribosomal protein S9
HPG27_77	0	13	0.533126	ribosomal protein L13
HPG27_78	1	12	0.770094	hypothetical protein
HPG27_79	0	9	-0.285784	malate:quinone oxidoreductase
HPG27_80	0	10	-0.470582	hypothetical protein
HPG27_81	2	12	-0.816957	RNA polymerase sigma-80 factor

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_53	GPOSANCHOR	28	0.034	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 28.1 bits (62), Expect = 0.034 Identities = 20/99 (20%), Positives = 36/99 (36%) Query: 2 KDLQDSKQVLENEKAELSKEKEILTKEKIELTEKNKALTTEKTELNNKIIGLDTEKERLE 61 + + + L EK L + EL + + T + KI L+ EK LE Sbjct: 235 EGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALE 294 Query: 62 RENKNLTTDKENLTTALSTAKSQAEQTSQKLNELERRHA 100 E +L + L + + + + + +LE H Sbjct: 295 AEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQ 333
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_54	GPOSANCHOR	37	4e-05	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 37.4 bits (86), Expect = 4e-05 Identities = 44/233 (18%), Positives = 74/233 (31%), Gaps = 11/233 (4%) Query: 4 LSSTREKLEARIGELENEKAELLREKDNLTKANTELYRERNDLVREKENLNNQLNELQKQ 63 L + + LE + N + L L + DL + E N + Sbjct: 118 LEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAK 177 Query: 64 VKELEQSQQVLKTEKAELLREKDNLTKANTELKTENDKLNHQVIALTKEQDSLKYERVQL 123 +K LE + L+ +AEL + + +T + L + AL + L+ Sbjct: 178 IKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGA 237 Query: 124 QDAHGFLEELCADLEKDNQHLTDKLKKLESAQKSLENSNDQLLQAIEKIAEEKTELEREM 183 + LE + L + +LE A + N + I+ + EK LE E Sbjct: 238 MNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEK 297 Query: 184 AHLKSLEATDKIELDLQNWRFKSAIEDLKRQNRKLEEENIALKERAYGLNEQL 236 A L+ + L+R E L+ L EQ Sbjct: 298 ADLEHQSQVLNANR-----------QSLRRDLDASREAKKQLEAEHQKLEEQN 339 Score = 28.9 bits (64), Expect = 0.018 Identities = 36/160 (22%), Positives = 59/160 (36%) Query: 4 LSSTREKLEARIGELENEKAELLREKDNLTKANTELYRERNDLVREKENLNNQLNELQKQ 63 L + + LEAR ELE + + L E+ L K +L L Sbjct: 181 LEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNF 240 Query: 64 VKELEQSQQVLKTEKAELLREKDNLTKANTELKTENDKLNHQVIALTKEQDSLKYERVQL 123 + L+ EKA L + L KA + + ++ L E+ +L+ E+ L Sbjct: 241 STADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADL 300 Query: 124 QDAHGFLEELCADLEKDNQHLTDKLKKLESAQKSLENSND 163 + L L +D + K+LE+ + LE N Sbjct: 301 EHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNK 340
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_67	UREASE	1044	0.0	Urea amidohydrolase (urease) protein signature.
>UREASE#Urea amidohydrolase (urease) protein signature. Length = 570 Score = 1044 bits (2701), Expect = 0.0 Identities = 353/569 (62%), Positives = 442/569 (77%), Gaps = 4/569 (0%) Query: 3 KISRKEYVSMYGPTTGDKVRLGDTDLIAEVEHDYTIYGEELKFGGGKTLREGMSQSN-NP 61 ++SR Y +M+GPT GDKVRL DT+L EVE D+T +GEE+KFGGGK +R+GM QS Sbjct: 4 RMSRAAYANMFGPTVGDKVRLADTELFIEVEKDFTTHGEEVKFGGGKVIRDGMGQSQVTR 63 Query: 62 SKEELDLIITNALIVDYTGIYKADIGIKDGKIAGIGKGGNKDMQDGVKNNLSVGPATEAL 121 +D +ITNALI+D+ GI KADIG+KDG+IA IGK GN DMQ GV + VGP TE + Sbjct: 64 EGGAVDTVITNALILDHWGIVKADIGLKDGRIAAIGKAGNPDMQPGV--TIIVGPGTEVI 121 Query: 122 AGEGLIVTAGGIDTHIHFISPQQIPTAFASGVTTMIGGGTGPADGTNATTITPGRRNLKW 181 AGEG IVTAGG+D+HIHFI PQQI A SG+T M+GGGTGPA GT ATT TPG ++ Sbjct: 122 AGEGKIVTAGGMDSHIHFICPQQIEEALMSGLTCMLGGGTGPAHGTLATTCTPGPWHIAR 181 Query: 182 MLRAAEEYSMNLGFLAKGNTSNDASLADQIEAGAIGFKIHEDWGTTPSAINHALDVADKY 241 M+ AA+ + MNL F KGN S +L + + GA K+HEDWGTTP+AI+ L VAD+Y Sbjct: 182 MIEAADAFPMNLAFAGKGNASLPGALVEMVLGGATSLKLHEDWGTTPAAIDCCLSVADEY 241 Query: 242 DVQVAIHTDTLNEAGCVEDTMAAIAGRTMHTFHTEGAGGGHAPDIIKVAGEHNILPASTN 301 DVQV IHTDTLNE+G VEDT+AAI GRT+H +HTEGAGGGHAPDII++ G+ N++P+STN Sbjct: 242 DVQVMIHTDTLNESGFVEDTIAAIKGRTIHAYHTEGAGGGHAPDIIRICGQPNVIPSSTN 301 Query: 302 PTIPFTVNTEAEHMDMLMVCHHLDKSIKEDVQFADSRIRPQTIAAEDTLHDMGIFSITSS 361 PT P+TVNT AEH+DMLMVCHHL +I ED+ FA+SRIR +TIAAED LHD+G FSI SS Sbjct: 302 PTRPYTVNTLAEHLDMLMVCHHLSPTIPEDIAFAESRIRKETIAAEDILHDIGAFSIISS 361 Query: 362 DSQAMGRVGEVITRTWQTADKNKKEFGRLKEEKGDNDNFRIKRYLSKYTINPAIAHGISE 421 DSQAMGRVGEV RTWQTADK K++ GRLKEE GDNDNFR+KRY++KYTINPAIAHG+S Sbjct: 362 DSQAMGRVGEVAIRTWQTADKMKRQRGRLKEETGDNDNFRVKRYIAKYTINPAIAHGLSH 421 Query: 422 YVGSVEVGKVADLVLWSPAFFGVKPNMIIKGGFIALSQMGDANASIPTPQPVYYREMFAH 481 +GS+EVGK ADLVLW+PAFFGVKP+M++ GG IA + MGD NASIPTPQPV+YR MF Sbjct: 422 EIGSLEVGKRADLVLWNPAFFGVKPDMVLLGGTIAAAPMGDPNASIPTPQPVHYRPMFGA 481 Query: 482 HGKAKYDANITFVSQAAYDKGIKEELGLERQVLPVKNCR-NITKKDMQFNDTTAHIEVNP 540 +G+++ ++++TFVSQA+ D G+ LG+ ++++ V+N R I K M N T HIEV+P Sbjct: 482 YGRSRTNSSVTFVSQASLDAGLAGRLGVAKELVAVQNTRGGIGKASMIHNSLTPHIEVDP 541 Query: 541 ETYHVFVDGKEVTSKPANKVSLAQLFSIF 569 ETY V DG+ +T +PA + +AQ + +F Sbjct: 542 ETYEVRADGELLTCEPATVLPMAQRYFLF 570
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_81	IGASERPTASE	32	0.007	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 32.3 bits (73), Expect = 0.007 Identities = 21/154 (13%), Positives = 50/154 (32%), Gaps = 9/154 (5%) Query: 7 EEKAPKRAKQEAKTEATQENKAKENNKENKNNKAKESKIKENKTKESKIKEAKAKEPIPV 66 + + A+ ++T+ TQ + KE K KAK E + K + Sbjct: 1079 NTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVE-------TEKTQEVPKVTSQVSP 1131 Query: 67 KKLSFNEELEELFANSLSDCVSYESIIQISAKVPTLAQIKKIKELCQKYQKKLVSSSEYA 126 K+ + A + +I + ++ T A ++ + ++ V+ S Sbjct: 1132 KQEQSETVQPQ--AEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTV 1189 Query: 127 KKLNAIDKIKKTEEKQKVLDEELEDGYDFLKEKD 160 N++ + + + + K + Sbjct: 1190 NTGNSVVENPENTTPATTQPTVNSESSNKPKNRH 1223

HPG27_175

HPG27_186

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_175	-1	11	3.429460	fumarate reductase, iron-sulfur subunit
HPG27_176	-1	11	3.383571	fumarate reductase. flavo protein subunit
HPG27_177	-2	13	2.103349	fumarate reductase, cytochrome b subunit
HPG27_178	-2	15	2.136938	triose phosphate isomerase
HPG27_179	-2	17	3.420370	enoyl-(acyl-carrier-protein) reductase
HPG27_180	-2	15	3.399687	UDP-3-0-(3-hydroxymyristoyl) glucosamine
HPG27_181	-2	17	3.232400	S-adenosyl methionine synthetase
HPG27_182	-2	18	2.272373	nucleoside diphosphate kinase
HPG27_183	0	13	-2.967948	hypothetical protein
HPG27_184	0	11	-1.956319	fatty acid/phospholipid synthesis protein
HPG27_185	0	11	-2.865850	beta-ketoacyl-acylcarrier protein synthase III
HPG27_186	1	11	-3.097137	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_178	TYPE4SSCAGA	30	0.013	Type IV secretion system CagA exotoxin signature.
>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature. Length = 1147 Score = 29.7 bits (66), Expect = 0.013 Identities = 19/48 (39%), Positives = 26/48 (54%), Gaps = 1/48 (2%) Query: 123 GEELTTREKGFRAVKEFLNEQLENIDLNYSNLIVAYEPIWAIGTKKSA 170 E TT K F +K+ LN +L N + N +N + EPI+A KK A Sbjct: 855 QAEATTLSKNFSDIKKELNAKLGNFN-NNNNNGLKNEPIYAKVNKKKA 901
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_179	DHBDHDRGNASE	59	3e-12	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 58.9 bits (142), Expect = 3e-12 Identities = 60/263 (22%), Positives = 109/263 (41%), Gaps = 29/263 (11%) Query: 4 LKGKKGLIVGVANNKSIAYGIAQSCFNQGATL-AFTYLNESLEKRVRPIAQELNSPYVYE 62 ++GK I G A + I +A++ +QGA + A Y E LEK V + E + Sbjct: 6 IEGKIAFITGAA--QGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP 63 Query: 63 LDVSKEEHFKPLYDSVKKDLGSLDFIVHSVAF--------APKEALEGSLLETSKSAFNT 114 DV + +++++G +D +V+ E E + S FN Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNA 123 Query: 115 AMEISVYSLIELTNTLKPLLNNGASVLTLSYLGSTKYMAHYNVMGLAKAALESAVRYLAV 174 + +S Y + + ++ + +N A V S MA Y +KAA + L + Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGVPRTS-------MAAY---ASSKAAAVMFTKCLGL 173 Query: 175 DLGKHHIRVNALSAGPIRT-----LASSGIADFRMILKWNE---INAPLRKNVSLEEVGN 226 +L +++IR N +S G T L + ++I E PL+K ++ + Sbjct: 174 ELAEYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIAD 233 Query: 227 AGMYLLSSLSSGVSGEVHFVDAG 249 A ++L+S + ++ VD G Sbjct: 234 AVLFLVSGQAGHITMHNLCVDGG 256

HPG27_299

HPG27_318

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_299	2	15	-1.374014	hypothetical protein
HPG27_300	1	16	-1.515638	arginyl-tRNA synthetase
HPG27_301	2	14	-1.148687	hypothetical protein
HPG27_302	1	13	-1.044886	5'-guanylate kinase
HPG27_303	1	13	-1.294420	polyE-rich protein
HPG27_304	-1	14	-2.253231	membrane bound endonuclease
HPG27_305	1	13	-2.163788	outer membrane protein
HPG27_306	4	15	-2.425715	flagellar basal-body L-ring protein
HPG27_307	4	13	-2.029047	CMP-N-acetyl neuraminic acid synthetase
HPG27_308	3	12	-1.220703	CMP-N-acetyl neuraminic acid synthetase
HPG27_309	3	12	-0.882256	flagellar biosynthesis protein G
HPG27_310	2	13	0.645763	putative tetraacyl disaccharide-1-P4-kinase
HPG27_311	2	14	1.264146	NH(3)-dependent NAD+ synthetase
HPG27_312	-1	14	0.567613	*ketol-acid reducto isomerase
HPG27_313	-1	15	-0.633917	cell division inhibitor
HPG27_314	0	14	-1.911604	cell division topological specificity factor
HPG27_315	0	12	-1.520878	DNA processing chain A
HPG27_316	0	15	-2.369841	Holliday junction resolvase-like protein
HPG27_317	1	17	-1.928572	cysteine-rich protein C
HPG27_318	2	19	-0.463101	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_302	PF05272	29	0.011	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 29.3 bits (65), Expect = 0.011 Identities = 9/18 (50%), Positives = 11/18 (61%) Query: 8 LILSGPSGAGKSTLTKYL 25 ++L G G GKSTL L Sbjct: 599 VVLEGTGGIGKSTLINTL 616
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_303	IGASERPTASE	67	1e-13	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 66.6 bits (162), Expect = 1e-13 Identities = 55/266 (20%), Positives = 85/266 (31%), Gaps = 18/266 (6%) Query: 140 ELENLGDLEALAKEEPNNEEQLLPTLDAQEEKEEVKETPQEEKEEVKETPQEEKEEVKET 199 E+E N Q +E + TP E E V E Sbjct: 984 EVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAEN 1043 Query: 200 -PQEEKPKDDETQEGDETPKDEEVSKELETQEKLEIPKEETQKEVKEEIKE--ETQEQEP 256 QE K + Q+ ET ++E+ + K + EV + E ETQ E Sbjct: 1044 SKQESKTVEKNEQDATETTAQ---NREVAKEAKSNVKANTQTNEVAQSGSETKETQTTET 1100 Query: 257 IKEETQENKEEKQEETQDSPSTQELEAMQELVKEIQENSNGQENKEKTQESAEALQETQA 316 + T E +E+ + ET E QE+ K + S QE E Q AE +E Sbjct: 1101 KETATVEKEEKAKVET---------EKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDP 1151 Query: 317 HELEKQEIAETPQELEIPQAQEK---ETPQEETQEKETPKDESMQESAQNLQDKETPQEE 373 K+ ++T + Q ++ Q T+ S+ E+ +N T Sbjct: 1152 TVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTV 1211 Query: 374 TQEDHYESIEDIPEPVMAKAMGEELP 399 E + V + E Sbjct: 1212 NSESSNKPKNRHRRSVRSVPHNVEPA 1237 Score = 63.2 bits (153), Expect = 1e-12 Identities = 35/234 (14%), Positives = 83/234 (35%), Gaps = 9/234 (3%) Query: 148 EALAKEEPNNEEQLLPTLDAQEEKEEVKETPQEEKEEVK-----ETPQEEKEEVKETPQE 202 E +A+ + P ++ + + + QE K K + EV + + Sbjct: 1015 EEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKS 1074 Query: 203 EKPKDDETQEGDETPKDEEVSKELETQEKLEIPKEETQKEVKEEIKEETQEQEPIKEETQ 262 + +T E ++ + + ++ ET+E + KEE K E+ +E + + + Q Sbjct: 1075 NVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPK-Q 1133 Query: 263 ENKEEKQEETQDSPSTQELEAMQELVKEIQENSNGQENKEKTQESAEALQETQAHELEKQ 322 E E Q + + + ++E + ++ ++ ++T + E Sbjct: 1134 EQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGN 1193 Query: 323 EIAETPQELEIPQAQEKETPQEETQEKETPKD-ESMQESAQNLQDKETPQEETQ 375 + E P+ Q E+ K + S++ N++ T + Sbjct: 1194 SVVENPENTTPATTQPTVN--SESSNKPKNRHRRSVRSVPHNVEPATTSSNDRS 1245 Score = 49.3 bits (117), Expect = 3e-08 Identities = 36/186 (19%), Positives = 71/186 (38%), Gaps = 25/186 (13%) Query: 142 ENLGDLEALAKEEPNNEEQLLPTLDAQEEKEEVKET-PQEEKE----EVKETPQEEKEEV 196 E +AKE +N + T + + E KET E KE E +E + E E+ Sbjct: 1060 ETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKT 1119 Query: 197 KETPQ---EEKPKDDETQ----------EGDETPKDEEVSKELETQEKLEIPKEETQKEV 243 +E P+ + PK ++++ E D T +E + T E P +ET V Sbjct: 1120 QEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNV 1179 Query: 244 KEEIKEETQ-------EQEPIKEETQENKEEKQEETQDSPSTQELEAMQELVKEIQENSN 296 ++ + E T + P + E+ + P + +++ + ++ + Sbjct: 1180 EQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATT 1239 Query: 297 GQENKE 302 ++ Sbjct: 1240 SSNDRS 1245
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_306	FLGLRINGFLGH	191	3e-63	Flagellar L-ring protein signature.
>FLGLRINGFLGH#Flagellar L-ring protein signature. Length = 232 Score = 191 bits (486), Expect = 3e-63 Identities = 51/172 (29%), Positives = 84/172 (48%), Gaps = 18/172 (10%) Query: 56 GERPLFADRRAMKPNDLITIIVSEKASANYSSS----KDYKSASGGNSTPPRLTYNGLDE 111 G +PLF DRR D +TI++ E SA+ SSS +D K+ G ++ P L GL Sbjct: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYL--QGLFG 118 Query: 112 RKKQEAQYLDDKNNYNFTKSSNNTNFKGGGSQKKSEDLEIVLSARIIKVLENGNYFIYGN 171 + + + S F G G S L+ + +VL NGN + G Sbjct: 119 NARADVEA------------SGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGE 166 Query: 172 KEVLVDGEKQILKVSGVIRPYDIERNNTIQSKFLADAKIEYTNLGHLSDSNK 223 K++ ++ + ++ SGV+ P I +NT+ S +ADA+IEY G+++++ Sbjct: 167 KQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN 218
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_309	SACTRNSFRASE	28	0.018	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 28.0 bits (62), Expect = 0.018 Identities = 14/49 (28%), Positives = 21/49 (42%), Gaps = 3/49 (6%) Query: 102 KGETILKALECIAFE---EFQLHSLHLEVMENNFKAIAFYEKNHYELEG 147 + + + AL A E E L LE + N A FY K+H+ + Sbjct: 102 RKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGA 150

HPG27_431

HPG27_447

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_431	0	12	-3.140811	molybdenum ABC transporter
HPG27_432	0	11	-3.516260	molybdenum ABC transporter
HPG27_433	-1	8	-1.833578	molybdenum ABCtransporter ModD
HPG27_434	-1	9	-2.068837	glutamyl-tRNA synthetase
HPG27_435	-1	11	-2.879574	outer membrane protein
HPG27_436	-1	11	-2.545407	type II adenine specific methyltransferase
HPG27_437	0	14	-1.672110	DD-heptosyl transferase
HPG27_438	0	15	-1.103501	GTP-binding protein
HPG27_439	1	18	-3.146288	type II adenine specific DNA methyltransferase
HPG27_440	4	18	-0.093327	type II restriction endonuclease
HPG27_441	6	17	0.417518	type II DNA modification enzyme
HPG27_442	1	17	0.156916	hypothetical protein
HPG27_443	2	18	-0.011600	hypothetical protein
HPG27_444	2	20	0.203536	catalase-like protein
HPG27_445	2	18	0.394127	outer membrane protein
HPG27_446	3	17	-0.694333	outer membrane protein
HPG27_447	5	19	-1.437930	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_433	PF05272	31	0.007	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 30.8 bits (69), Expect = 0.007 Identities = 11/23 (47%), Positives = 14/23 (60%) Query: 30 VVALLGESGAGKSTILRILAGLE 52 V L G G GKST++ L GL+ Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLD 620
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_438	TCRTETOQM	197	2e-57	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 197 bits (503), Expect = 2e-57 Identities = 112/453 (24%), Positives = 186/453 (41%), Gaps = 66/453 (14%) Query: 3 NIRNIAVIAHVDHGKTTLVDGLLSQSGTFSEREKVDE--RVMDSNDLERERGITILSKNT 60 I NI V+AHVD GKTTL + LL SG +E VD+ D+ LER+RGITI + T Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61 Query: 61 AIYYKDTKINIIDTPGHADFGGEVERVLKMVDGVLLLVDAQEGVMPQTKFVVKKALSFGI 120 + +++TK+NIIDTPGH DF EV R L ++DG +LL+ A++GV QT+ + GI Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121 Query: 121 CPIVVVNKIDKPAAEPDRVVDEVFDLF---------VAMGASDKQLDFPV-----VYAAA 166 I +NKID+ + V ++ + V + + +F Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG 181 Query: 167 RDGYAMKSLDDE----------------------------KKNL--EPLFETILEHVPSP 196 D K + + K N+ + L E I S Sbjct: 182 NDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSS 241 Query: 197 SGSVDEPLQMQIFTLDYDNYVGKIGIARVFNGSVKKNESVLLMKSDGSKENGRITKLIGF 256 + L ++F ++Y ++ R+++G + +SV + KE +IT++ Sbjct: 242 THRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRI----SEKEKIKITEMYTS 297 Query: 257 LGLARTEIENAYAGDIVAIAG--FNAMDV-GDSVVDPTNPMPLDPMHLEEPTMSVYFAVN 313 + +I+ AY+G+IV + V GD+ + P +P P + + Sbjct: 298 INGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENP----LPLLQTTVEPS 353 Query: 314 DSPLAGLEGKHVTANKLKDRLLKEMQTNIAMKCEEMGEGKFKVSGRGELQITILAENLRR 373 + + D LL+ + + +S G++Q+ + L+ Sbjct: 354 KPQQREMLLDALLEISDSDPLLRYYVDSAT--------HEIILSFLGKVQMEVTCALLQE 405 Query: 374 E-GFEFSISRPEVIIKEENGVKCEPFEHLVIDT 405 + E I P VI E K E H+ + Sbjct: 406 KYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPP 438 Score = 40.2 bits (94), Expect = 2e-05 Identities = 20/80 (25%), Positives = 29/80 (36%), Gaps = 1/80 (1%) Query: 396 EPFEHLVIDTPQDSSGAIIERLGKRKAEMKAMNPMSDGYTRLEFEIPARGLIGYRSEFLT 455 EP+ I PQ+ K A + + + L EIPAR + YRS+ Sbjct: 537 EPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQ-LKNNEVILSGEIPARCIQEYRSDLTF 595 Query: 456 DTKGEGVMNHSFLEFRPFSG 475 T G V + +G Sbjct: 596 FTNGRSVCLTELKGYHVTTG 615
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_447	PF07201	32	0.008	Hypersensitivity response secretion protein HrpJ
>PF07201#Hypersensitivity response secretion protein HrpJ Length = 293 Score = 31.7 bits (72), Expect = 0.008 Identities = 17/129 (13%), Positives = 47/129 (36%), Gaps = 13/129 (10%) Query: 5 KALNE---ATAGAALKYHIQRALERSHTISEFSKQLELSAKNSKFSNATMRKIEEITQGV 61 +L++ + + A + ++ + + E ++ +S S SN+ + ++ + Sbjct: 66 LSLDKRKLSDSQARVSDVEEQVNQYLSKVPELEQKQNVSELLSLLSNSPNISLSQLKAYL 125 Query: 62 KSAKENIAKQEKALQDAITPLKQFGKNYPEFALKPNEALEKLLQEKNGQV---------A 112 + E ++Q K L LK + + +AL + +E+ + A Sbjct: 126 EGKSEEPSEQFKMLCGLRDALKGRPEL-AHLSHLVEQALVSMAEEQGETIVLGARITPEA 184 Query: 113 GAAFRDDLG 121 + + Sbjct: 185 YRESQSGVN 193

HPG27_477

HPG27_506

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_477	3	14	-2.387099	conserved hypothetical secreted protein
HPG27_478	6	16	-2.263265	hypothetical protein
HPG27_479	9	17	-2.185129	cag pathogenicity island protein 1
HPG27_480	9	17	-2.538103	hypothetical protein
HPG27_481	10	18	-2.854844	cag pathogenicity island protein 3
HPG27_482	8	21	-3.314270	cag pathogenicity island protein 4
HPG27_483	9	22	-3.512560	cag pathogenicity island protein 5
HPG27_484	9	25	-4.348719	virB11-like cag pathogenicity islandencoded
HPG27_485	9	27	-4.350145	cag pathogenicity island protein Z
HPG27_487	10	27	-4.316961	cag pathogenicity island protein X
HPG27_488	10	30	-4.067789	cag pathogenicity island protein W
HPG27_489	13	29	-4.614261	cag pathogenicity island protein V
HPG27_490	12	29	-4.728489	cag pathogenicity island protein U
HPG27_491	13	24	-4.683773	cag pathogenicity island protein T
HPG27_492	10	24	-5.123185	cag pathogenicity island protein S
HPG27_493	6	20	-3.796607	cag pathogenicity island protein Q
HPG27_494	7	18	-3.022645	hypothetical protein
HPG27_495	6	18	-2.674773	cag pathogenicity island protein M
HPG27_496	5	20	-2.151059	cag pathogenicity island protein N
HPG27_497	5	22	-2.854736	cag pathogenicity island protein L
HPG27_498	5	21	-2.837745	cag pathogenicity island protein I
HPG27_499	7	21	-3.835977	cag pathogenicity island protein H
HPG27_500	7	22	-4.053021	hypothetical protein
HPG27_501	8	22	-4.379754	cag pathogenicity island protein G
HPG27_502	7	22	-3.124512	cag pathogenicity island protein F
HPG27_503	7	23	-2.597252	cag pathogenicity island protein E
HPG27_504	5	22	-1.881618	cag pathogenicity island protein D
HPG27_505	3	17	-0.372828	cag pathogenicity island protein C
HPG27_506	3	17	-0.533694	cag pathogenicity island protein B

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_481	PF07201	30	0.022	Hypersensitivity response secretion protein HrpJ
>PF07201#Hypersensitivity response secretion protein HrpJ Length = 293 Score = 29.8 bits (67), Expect = 0.022 Identities = 14/76 (18%), Positives = 26/76 (34%), Gaps = 15/76 (19%) Query: 277 APENSKEKLIEELIANSQLIANEEEREKKLLAEKEKQ--------EAELAKY--KLKDLE 326 S + EE+ E +E L K E ++ +Y K+ +LE Sbjct: 44 GTLQSIADMAEEVTF-----VFSERKELSLDKRKLSDSQARVSDVEEQVNQYLSKVPELE 98 Query: 327 NQKKLKALEAELKKKN 342 ++ + L + L Sbjct: 99 QKQNVSELLSLLSNSP 114
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_487	TYPE4SSCAGX	867	0.0	Type IV secretion system CagX conjugation protein si...
>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein signature. Length = 522 Score = 867 bits (2241), Expect = 0.0 Identities = 511/522 (97%), Positives = 514/522 (98%), Gaps = 1/522 (0%) Query: 1 MGRALFKKIVGCFCLGYLFLSSVIEAAP-DIKNFNRGRVKVVNKKIAYLGDEKPITIWTS 59 MG+A FKKIVGCFCLGYLFLSS IEA DIKNFNRGRVKVVNKKIAYLGDEKPITIWTS Sbjct: 1 MGQAFFKKIVGCFCLGYLFLSSAIEAVALDIKNFNRGRVKVVNKKIAYLGDEKPITIWTS 60 Query: 60 LDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTR 119 LDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTR Sbjct: 61 LDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTR 120 Query: 120 DYQEFLKTKKLIVDAPDPKELEEQKKALEKEKEAKEQAQKVQKDKREKRKEERAKNRANL 179 DYQEFLKTKKLIVDAPDPKELEEQKKALEKEKEAKEQAQK QKDKREKRKEERAKNRANL Sbjct: 121 DYQEFLKTKKLIVDAPDPKELEEQKKALEKEKEAKEQAQKAQKDKREKRKEERAKNRANL 180 Query: 180 ENLTNAMSNPQNLSNNKNLSELIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQA 239 ENLTNAMSNPQNLSNNKNLSELIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQA Sbjct: 181 ENLTNAMSNPQNLSNNKNLSELIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQA 240 Query: 240 EETIKQRAKDKISIKTDKSQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKD 299 EE ++QRAKDKISIKTDKSQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKD Sbjct: 241 EEAVRQRAKDKISIKTDKSQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKD 300 Query: 300 NFASAYLTVKLEYPQRHEVSSVIEEELKKREEAKRQRELIKQENLNTTAYINRVMMASNE 359 NFASAYLTVKLEYPQRHEVSSVIEEELKKREEAKRQRELIKQENLNTTAYINRVMMASNE Sbjct: 301 NFASAYLTVKLEYPQRHEVSSVIEEELKKREEAKRQRELIKQENLNTTAYINRVMMASNE 360 Query: 360 QIINKEKIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIF 419 QIINKEKIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIF Sbjct: 361 QIINKEKIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIF 420 Query: 420 DDGTFTYFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNLGLRWYRVNEIAEKFKLIK 479 DDGTFTYFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTN GLRWYRVNEIAEKFKLIK Sbjct: 421 DDGTFTYFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIK 480 Query: 480 DKALVTVINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK 521 DKALVTVINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK Sbjct: 481 DKALVTVINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK 522
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_489	PF04335	118	6e-35	VirB8 type IV secretion protein
>PF04335#VirB8 type IV secretion protein Length = 227 Score = 118 bits (298), Expect = 6e-35 Identities = 44/205 (21%), Positives = 74/205 (36%), Gaps = 10/205 (4%) Query: 27 KLNKANRTFKRAFYL---SMALNVAAVTSIVMMMPLKKTDIFVYGIDRYTGEFKIVKRSD 83 KL A R+ K A+ + + AL A V ++ + PLK + +V +DR TGE I + Sbjct: 24 KLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVDRNTGEASIAAKLH 83 Query: 84 A-RQIVNSEAVVDSATSKFVSLLFGYSKNSLRDRKDQLMQYCDVSFQTQAMRMFNENIRQ 142 I EAV + +V G+ + + D +M Q + R + + Q Sbjct: 84 GDATITYDEAVRKYFLATYVRYREGWIAAAREEYFDAVMVMSARPEQDRWSRFYKTDNPQ 143 Query: 143 FVDKVRA-EAIISSNIQREKVKNSPLTRLTFFITIKITPDTMENYEYITKKQVTIYYDFA 201 + A + I + +F +T T TI Y Sbjct: 144 SPQNILANRTDVFVEI-KRVSFLGGNVAQVYFTKESVTGSNS----TKTDAVATIKYKVD 198 Query: 202 RGNSSQENLIINPFGFKVFDIQITD 226 S + + NP G++V + Sbjct: 199 GTPSKEVDRFKNPLGYQVESYRADV 223
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_496	TYPE4SSCAGX	32	0.004	Type IV secretion system CagX conjugation protein si...
>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein signature. Length = 522 Score = 31.7 bits (71), Expect = 0.004 Identities = 30/119 (25%), Positives = 56/119 (47%), Gaps = 16/119 (13%) Query: 24 AINTALLPSEYKELVALGFKKIKTLHQRHDDEEVTEEEKEFATNALREKLRNDRARAEQI 83 A+N AL+ +Y+E + K K + D +E+ E++K EK + + +A++ Sbjct: 112 AVNFALMTRDYQEFL----KTKKLIVDAPDPKELEEQKKAL------EKEKEAKEQAQKA 161 Query: 84 QKNIEAFEKKNNSSIQKKAAKHKGLQELNEINANPLNGNPNSNSSTETKSNKDDNFDEM 142 QK+ K +++A L+ L +NP N + N N S K +++ D+M Sbjct: 162 QKD------KREKRKEERAKNRANLENLTNAMSNPQNLSNNKNLSELIKQQRENELDQM 214
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_503	ACRIFLAVINRP	33	0.008	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 32.9 bits (75), Expect = 0.008 Identities = 20/88 (22%), Positives = 32/88 (36%), Gaps = 18/88 (20%) Query: 19 EVQKRQFQKIEELKADMQKGVNPFFKVLFDGGNRLFGFPETFIYSSI-------FILFVT 71 + K K+ EL+ +G+ +D F+ SI F + Sbjct: 301 DTAKAIKAKLAELQPFFPQGMK--VLYPYD--------TTPFVQLSIHEVVKTLFEAIML 350 Query: 72 IVLSVILF-QAYEPVLIVAIVIVLVALG 98 + L + LF Q LI I + +V LG Sbjct: 351 VFLVMYLFLQNMRATLIPTIAVPVVLLG 378

HPG27_635

HPG27_652

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_635	2	9	0.906197	methylated-DNA--protein-cysteine methyl
HPG27_636	1	9	1.402144	hypothetical protein
HPG27_637	0	9	1.351182	putative lipopolysaccharide biosynthesis
HPG27_638	3	10	1.051201	ribonucleoside-diphosphatereductase 1 alpha
HPG27_639	3	11	0.695797	hypothetical protein
HPG27_640	2	12	0.187464	hypothetical protein
HPG27_641	1	10	0.672512	UDP-N-acetylglucosamine pyrophosphorylase
HPG27_642	1	11	0.725452	flagellar biosynthetic protein
HPG27_643	2	10	1.376449	iron (III) dicitrate transport protein
HPG27_644	-2	10	2.011676	iron (II) transport protein
HPG27_645	1	13	2.411122	hypothetical protein
HPG27_646	3	12	3.509232	acetyl coenzyme A acetyltransferase
HPG27_647	4	13	2.957590	succinyl-CoA-transferase subunit A
HPG27_648	4	13	2.555194	succinyl-CoA-transferase subunit B
HPG27_649	3	13	1.400898	short-chain fatty acids transporter
HPG27_650	3	13	1.210480	putative outer membrane protein
HPG27_652	2	12	1.536985	N-methyl hydantoinase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_640	PF07132	33	9e-05	Harpin protein (HrpN)
>PF07132#Harpin protein (HrpN) Length = 356 Score = 33.1 bits (75), Expect = 9e-05 Identities = 19/45 (42%), Positives = 31/45 (68%) Query: 21 IGGGVGAGMGGAMGGMIGALGGPWGTVFGAGIGGGIGAYSGAEIG 65 +G +G G+GG +GG+ +LGG G + G G+GGG+G+ G+ +G Sbjct: 61 MGSMMGGGLGGGLGGLGSSLGGLGGGLLGGGLGGGLGSSLGSGLG 105 Score = 30.8 bits (69), Expect = 5e-04 Identities = 18/50 (36%), Positives = 28/50 (56%) Query: 17 LGRDIGGGVGAGMGGAMGGMIGALGGPWGTVFGAGIGGGIGAYSGAEIGD 66 +G +GGG+G G+GG + G GG G G G+G +G+ G+ +G Sbjct: 61 MGSMMGGGLGGGLGGLGSSLGGLGGGLLGGGLGGGLGSSLGSGLGSALGG 110
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_642	FLGBIOSNFLIP	279	2e-97	Escherichia coli: Flagellar biosynthetic protein Fl...
>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP signature. Length = 245 Score = 279 bits (716), Expect = 2e-97 Identities = 115/246 (46%), Positives = 164/246 (66%), Gaps = 3/246 (1%) Query: 12 ILRFFIFFILICPLICPLMSADSALPSVNLSLNAPNDPKQLVTTLNVIALLTLLVLAPSL 71 + R ++ LI PL A + LP + S P + + + +T L P++ Sbjct: 1 MRRLLSVAPVLLWLITPL--AFAQLPGIT-SQPLPGGGQSWSLPVQTLVFITSLTFIPAI 57 Query: 72 ILVMTSFTRLIVVFSFLRTALGTQQTPPTQILVSLSLILTFFIMEPSLKKAYDTGIKPYM 131 +L+MTSFTR+I+VF LR ALGT PP Q+L+ L+L LTFFIM P + K Y +P+ Sbjct: 58 LLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFS 117 Query: 132 DKKISYTEAFEKSALPFKEFMLKNTREKDLALFFRIRNLPNPKTPDEVSLSVLIPAFMIS 191 ++KIS EA EK A P +EFML+ TRE DL LF R+ N + P+ V + +L+PA++ S Sbjct: 118 EEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTS 177 Query: 192 ELKTAFQIGFLLYLPFLVIDMVISSILMAMGMMMLPPVMISLPFKILVFILVDGFNLLTE 251 ELKTAFQIGF +++PFL+ID+VI+S+LMA+GMMM+PP I+LPFK+++F+LVDG+ LL Sbjct: 178 ELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVG 237 Query: 252 NLVASF 257 +L SF Sbjct: 238 SLAQSF 243

HPG27_669

HPG27_686

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_669	2	12	-1.310846	hypothetical protein
HPG27_670	3	12	-0.647269	RNA polymerase sigma-54 factor
HPG27_671	0	12	0.695395	ABC transporter. ATP-binding protein
HPG27_672	1	13	1.525869	hypothetical protein
HPG27_674	1	11	2.530620	hypothetical protein
HPG27_675	1	14	2.645587	hypothetical protein
HPG27_676	2	13	2.569350	hypothetical protein
HPG27_677	1	13	2.587614	outer membrane protein
HPG27_678	1	12	2.211850	anaerobic C4-dicarboxylate transport protein
HPG27_679	-1	9	0.609632	L-asparaginaseII
HPG27_680	-1	10	-0.581758	outer membrane protein
HPG27_681	0	14	-2.322215	outer membrane protein
HPG27_682	2	14	-2.830025	transcriptional regulator
HPG27_683	3	17	-3.913518	hypothetical protein
HPG27_684	3	19	-4.345884	hypothetical protein
HPG27_685	2	13	-2.748343	hypothetical protein
HPG27_686	2	13	-2.521921	labile enterotoxin outputA

HPG27_824

HPG27_831

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_824	2	13	2.984996	flagellar hook protein
HPG27_825	1	14	2.335264	CDP-diglyceride hydrolase
HPG27_826	2	14	2.413254	alkylphosphonate uptake protein
HPG27_827	2	14	2.276324	hypothetical protein
HPG27_828	3	14	2.443775	hypothetical protein
HPG27_829	3	15	2.338402	catalase
HPG27_830	1	13	2.120941	iron-regulated outer membrane protein
HPG27_831	2	15	-0.175202	Holliday junction endodeoxyribonuclease

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_824	FLGHOOKAP1	42	7e-06	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 41.9 bits (98), Expect = 7e-06 Identities = 13/49 (26%), Positives = 27/49 (55%) Query: 669 SISGSKLESSNVDLSRSLTNLIVVQRGFQANSKAVTTSDQILNTLLNLK 717 +S + S V+L NL Q+ + AN++ + T++ I + L+N++ Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546 Score = 39.2 bits (91), Expect = 5e-05 Identities = 11/35 (31%), Positives = 20/35 (57%) Query: 4 SLWSGVNGMQAHQIALDIESNNIANVNTTGFKYSR 38 + + ++G+ A Q AL+ SNNI++ N G+ Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQT 37

HPG27_926

HPG27_944

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_926	2	19	1.191584	cell division protein
HPG27_927	0	26	-1.253331	hypothetical protein
HPG27_928	1	21	-1.221701	hypothetical protein
HPG27_929	1	21	-1.430299	hypothetical protein
HPG27_930	1	23	-2.152858	exonuclease VII, large subunit
HPG27_931	3	24	-4.367621	hypothetical protein
HPG27_932	3	21	-4.519675	hypothetical protein
HPG27_933	2	22	-5.213466	hypothetical protein
HPG27_934	3	31	-6.942875	hypothetical protein
HPG27_935	3	29	-6.392727	hypothetical protein
HPG27_936	3	23	-6.241509	hypothetical protein
HPG27_937	5	23	-5.695246	hypothetical protein
HPG27_938	4	23	-5.675879	hypothetical protein
HPG27_939	3	22	-5.463252	hypothetical protein
HPG27_940	2	21	-5.128619	hypothetical protein
HPG27_941	2	22	-5.541355	hypothetical protein
HPG27_942	2	17	-4.231345	protein kinase C-like protein
HPG27_943	0	16	-3.940982	protein phosphatase 2 C-like protein (ptc1)
HPG27_944	-1	13	-3.538961	hypothetical protein

HPG27_955

HPG27_984

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_955	0	10	-3.571769	hypothetical protein
HPG27_956	1	12	-3.646931	methionyl-tRNA synthetase
HPG27_957	3	13	-4.938274	cyclopropane fatty acid synthase
HPG27_958	6	20	-7.141902	hypothetical protein
HPG27_959	8	28	-8.789256	hypothetical protein
HPG27_960	7	24	-7.825898	hypothetical protein
HPG27_961	5	22	-6.551954	hypothetical protein
HPG27_962	6	21	-6.584808	hypothetical protein
HPG27_964	6	21	-6.682225	DNA topoisomerase I
HPG27_965	4	20	-6.291410	competence protein
HPG27_966	4	20	-6.354701	comB9-like competence protein
HPG27_967	3	27	-7.810470	competence protein
HPG27_968	5	29	-9.884437	hypothetical protein
HPG27_969	4	28	-8.507346	hypothetical protein
HPG27_970	3	27	-7.993041	type IV secretion system ATPase (virB11-like)
HPG27_971	4	26	-8.649272	hypothetical protein
HPG27_972	4	21	-7.680299	hypothetical protein
HPG27_973	4	19	-7.246184	hypothetical protein
HPG27_974	4	17	-6.143115	cag island protein,DNA transfer protein
HPG27_975	4	15	-3.879129	hypothetical protein
HPG27_976	4	14	-4.119591	hypothetical protein
HPG27_977	4	16	-4.233606	hypothetical protein
HPG27_978	4	16	-3.801398	hypothetical protein
HPG27_979	4	17	-4.134897	PARA protein
HPG27_980	3	19	-4.682522	adenine specific DNA methyltransferase
HPG27_981	4	27	-7.434198	hypothetical protein
HPG27_982	3	26	-6.951846	hypothetical protein
HPG27_983	2	21	-4.182791	periplasmic competence protein-like protein
HPG27_984	2	21	-5.218648	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_965	PF04335	98	3e-26	VirB8 type IV secretion protein
>PF04335#VirB8 type IV secretion protein Length = 227 Score = 98.0 bits (244), Expect = 3e-26 Identities = 35/202 (17%), Positives = 74/202 (36%), Gaps = 18/202 (8%) Query: 94 AERKIGDWIFSSAVFFFALALIEAIIIICLLPLKEKVPYLVTFSNATQNFAIVQR--ADK 151 +K+ + A ALA + + L PLK PY++T T +I + D Sbjct: 30 RSKKLAWVV---AGVAGALATAGVVAVAALTPLKTVEPYVITVDRNTGEASIAAKLHGDA 86 Query: 152 SIRANQALIRQLVASYVNNRE--NISNIKEQNEIAHETIRLQSAFEVWDFFEKLVSYEH- 208 +I ++A+ + +A+YV RE + +E + + + SA D + + ++ Sbjct: 87 TITYDEAVRKYFLATYVRYREGWIAAAREEY----FDAVMVMSARPEQDRWSRFYKTDNP 142 Query: 209 ----SIYTNINLTRKISIINIALISKTQANIEISAQLFNKEKLESEKRYRIIMTFEFKPI 264 +I N + I ++ + A + + + ++ + ++ Sbjct: 143 QSPQNILAN-RTDVFVEIKRVSFLGGNVAQVYFT-KESVTGSNSTKTDAVATIKYKVDGT 200 Query: 265 EIDTKSVPLNPTGFMVTGYDVT 286 NP G+ V Y Sbjct: 201 PSKEVDRFKNPLGYQVESYRAD 222
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_980	FbpA_PF05833	32	0.046	Fibronectin-binding protein
>FbpA_PF05833#Fibronectin-binding protein Length = 577 Score = 31.8 bits (72), Expect = 0.046 Identities = 47/235 (20%), Positives = 90/235 (38%), Gaps = 27/235 (11%) Query: 1238 EQDYEIIKDFMDKVGENNINLNEQTLNEYFIH-HPENILGRLSLEKTRY-SFETNGEQIY 1295 ++ E+ KD ++ N N T N F+ + N++ + +K +Y S E Y Sbjct: 229 KEIVEVCKDLFKEIQSNKFEFNCYTKNNSFVGFYCLNLMSKEDYKKIQYDSSSKLLENFY 288 Query: 1296 KY--ELQALEDKSLDLSQALNQAIEKLPKDVYQYHKTTLKTDALIIDANNERYQEVQKLI 1353 + L+ KS DL + + I + K + T K + + + ++ +L+ Sbjct: 289 YAKDKSDRLKSKSSDLQKIVMNNINRCTKKDKILNNTLKKCE------DKDIFKLYGELL 342 Query: 1354 K----NLERG-ELVKWDDLYFQLEQNNEMGIFLKPTKINSKVQDSRLKAYFKIKDALNDL 1408 L++G ++ + Y E + + I L K S+ S K Y K+K + Sbjct: 343 TANIYALKKGLSHIELANYY--SENYDTVKITLDENKTPSQNVQSYYKKYNKLKKSEEAA 400 Query: 1409 ------TSAELNPLSS---DLELESKRAKLNLVYDGFVKKFGYLNENKNRKDIKQ 1454 ELN L S ++ ++ + ++ GY+ K K K Sbjct: 401 NEQLLQNEEELNYLYSVLTNINNADNYDEIEEIKKELIET-GYIKFKKIYKSKKS 454
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_982	SECA	31	0.017	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 30.6 bits (69), Expect = 0.017 Identities = 31/169 (18%), Positives = 67/169 (39%), Gaps = 26/169 (15%) Query: 72 ELEELQQTITTDKTQQQLLEQDNIDFELQSALQNDLKDLDHLSDNKDKDDEEQAIQKSFE 131 ++ ++ +TI + ++ + + ID + ++ D+ L + + Sbjct: 668 DVSDVSETI---NSIREDVFKATIDAYIPPQSLEEMWDIPGLQERL----KNDFDLDLPI 720 Query: 132 QDLDDLQNDKLNLEIKEFINKQDDKNYQNKEQLNTETKENIRENSKN-----------SH 180 + D + + ++E I Q + YQ KE++ E +R K H Sbjct: 721 AEWLDKEPELHEETLRERILAQSIEVYQRKEEVVGA--EMMRHFEKGVMLQTLDSLWKEH 778 Query: 181 LIPITNLKNFLHNRRENFKVSQQDLPSEKQKKYSDQLFKKELLEYAKHN 229 L + L+ +H R +Q+D P ++ K+ S +F +LE K+ Sbjct: 779 LAAMDYLRQGIHLR----GYAQKD-PKQEYKRESFSMF-AAMLESLKYE 821
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_983	VACCYTOTOXIN	39	6e-05	Helicobacter pylori vacuolating cytotoxin signature.
>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature. Length = 1291 Score = 39.2 bits (91), Expect = 6e-05 Identities = 55/193 (28%), Positives = 75/193 (38%), Gaps = 29/193 (15%) Query: 139 NTAQTNATNDPMYANTPFSNGSDSSAYDNNPNSPNDNAIN--GKDGANGGNGYGIN-GND 195 N+AQ + PF+ G ++ N N+ D I G + N ++ G Sbjct: 368 NSAQKTEIQPTQVIDGPFAGGKNTVVNINRINTNADGTIRVGGFKASLTTNAAHLHIGKG 427 Query: 196 GINGSNGANGNNRNNSNNNAIGSGIDTDGVLGVDGVNGSNSSSGGSVGGYENNFT----- 250 GIN SN A+G R+ N G I DG L V+ G + +G S NF Sbjct: 428 GINLSNQASG--RSLLVENLTG-NITVDGPLRVNNQVGGYALAGSS-----ANFEFKAGT 479 Query: 251 ---NHGSTNNNTGEYDNFNN-------NSSSGGGLGNGGFFPIPFGNGGTN--NSNNPTN 298 N +T NN F N + G GNGGF + F +G TN N N Sbjct: 480 DTKNGTATFNNDISLGRFVNLKVDAHTANFKGIDTGNGGFNTLDF-SGVTNKVNINKLIT 538 Query: 299 SPTNGSSSNSATN 311 + TN + N N Sbjct: 539 ASTNVAVKNFNIN 551

HPG27_1043

HPG27_1056

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_1043	2	11	0.551371	glucose-6-phosphate1-dehydrogenase
HPG27_1044	3	9	0.890911	glucokinase
HPG27_1045	4	11	-0.108294	mannitol dehydrogenase
HPG27_1046	3	12	-0.143869	putative lipopolysaccharide biosynthesis
HPG27_1047	2	12	1.885565	hypothetical protein
HPG27_1048	3	12	2.699560	hypothetical protein
HPG27_1049	0	14	3.338655	outer membrane protein
HPG27_1050	0	12	2.647984	pyruvate ferredoxin oxidoreductase, gamma
HPG27_1051	-1	10	2.097802	pyruvate ferredoxin oxidoreductase, delta
HPG27_1052	-2	9	1.433192	pyruvate ferredoxin oxidoreductase, alpha
HPG27_1053	-2	12	0.397878	pyruvate ferredoxin oxidoreductase, beta
HPG27_1054	0	13	-0.565102	adenylosuccinate lyase
HPG27_1055	1	16	-1.509155	outer membrane protein
HPG27_1056	3	17	-1.563110	excinuclease ABC subunit B

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1050	YERSSTKINASE	29	0.010	Yersinia serine/threonine protein kinase signature.
>YERSSTKINASE#Yersinia serine/threonine protein kinase signature. Length = 732 Score = 29.3 bits (65), Expect = 0.010 Identities = 18/63 (28%), Positives = 33/63 (52%), Gaps = 9/63 (14%) Query: 50 YNRVDDEPILNHERFMQPDYVLVIDPGLVFIENIFANEKEDTTYIITSYLNKEELFEKKP 109 ++R ++P E F P+ + + N+ A+EK D ++++ L+ E FEK P Sbjct: 293 HSRSGEQPKGFTESFKAPE---------LGVGNLGASEKSDVFLVVSTLLHCIEGFEKNP 343 Query: 110 ELK 112 E+K Sbjct: 344 EIK 346

HPG27_1181

HPG27_1201

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_1181	-2	10	3.090782	carbamoyl-phosphate synthetase
HPG27_1182	-1	11	3.412842	formamidase
HPG27_1183	-1	11	2.476821	hypothetical protein
HPG27_1184	-1	11	2.617394	maf protein
HPG27_1185	0	10	2.765794	alanyl-tRNA synthetase
HPG27_1186	2	17	2.117494	hypothetical protein
HPG27_1187	-1	13	1.317286	outer membrane protein
HPG27_1188	2	13	-1.428396	hypothetical protein
HPG27_1189	1	11	-0.494308	ribosomal protein S18
HPG27_1190	2	10	-0.500050	single-strand DNA-binding protein
HPG27_1191	2	10	-0.414126	ribosomal protein S6
HPG27_1192	3	10	-0.216496	DNA polymerase III holoenzyme delta subunit
HPG27_1193	1	11	-0.178376	3'-5'exoribonuclease R
HPG27_1194	0	11	0.772888	shikimate 5-dehydrogenase
HPG27_1195	0	10	0.517669	hypothetical protein
HPG27_1196	0	11	0.982931	oligopeptide ABC transporter, permease protein
HPG27_1198	1	11	0.634660	tryptophanyl-tRNA synthetase
HPG27_1199	2	13	1.284348	biotin synthesis protein
HPG27_1200	3	16	1.972543	protein translocation protein
HPG27_1201	2	14	2.657136	ribosome releasing factor

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1195	IGASERPTASE	36	8e-05	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 35.8 bits (82), Expect = 8e-05 Identities = 19/88 (21%), Positives = 38/88 (43%), Gaps = 3/88 (3%) Query: 48 AEKTEIERQNSALSPKQEEANTTTTATEESPTKDTAPPLETTAQEKETKQETKQEQEKES 107 + E+ + S +SPKQE++ T E + D ++ + T +T+Q ++ S Sbjct: 1117 EKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETS 1176 Query: 108 EPKQNSVPPVQNNQKAPTISTMGKKPLE 135 N PV + T +++ + P Sbjct: 1177 ---SNVEQPVTESTTVNTGNSVVENPEN 1201 Score = 33.1 bits (75), Expect = 7e-04 Identities = 19/86 (22%), Positives = 37/86 (43%), Gaps = 3/86 (3%) Query: 38 KKDSAPISPNAEKTEIERQNSALSPKQEEANTTTTATEESPTKDTAPPLETTAQEKETKQ 97 + + + N E + + N + + E + + T+E+ T +T ET EKE K Sbjct: 1056 QDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETK---ETATVEKEEKA 1112 Query: 98 ETKQEQEKESEPKQNSVPPVQNNQKA 123 + + E+ +E + V P Q + Sbjct: 1113 KVETEKTQEVPKVTSQVSPKQEQSET 1138 Score = 28.5 bits (63), Expect = 0.023 Identities = 19/94 (20%), Positives = 31/94 (32%), Gaps = 16/94 (17%) Query: 55 RQNSALSPKQEEANTTTTATEESPTKDT-------------APPLETTAQEKETKQETKQ 101 + N E T TT T+E+ T + P + + K+ + ET Q Sbjct: 1081 QTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQ 1140 Query: 102 EQ---EKESEPKQNSVPPVQNNQKAPTISTMGKK 132 Q +E++P N P K+ Sbjct: 1141 PQAEPARENDPTVNIKEPQSQTNTTADTEQPAKE 1174
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1200	SECGEXPORT	49	3e-10	Protein-export SecG membrane protein signature.
>SECGEXPORT#Protein-export SecG membrane protein signature. Length = 110 Score = 49.2 bits (117), Expect = 3e-10 Identities = 25/84 (29%), Positives = 47/84 (55%), Gaps = 3/84 (3%) Query: 1 MTSALLGLQIVLAVLIVVVVLLQ--KSSSIGLGAYSGSNDSLFGAKGPASFMAKLTMFLG 58 M ALL + +++A+ +V +++LQ K + +G +G++ +LFG+ G +FM ++T L Sbjct: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 60 Query: 59 LLFVINTIALGYFYNKEYGKSILD 82 LF I ++ LG N + Sbjct: 61 TLFFIISLVLGNI-NSNKTNKGSE 83

HPG27_1389

HPG27_1400

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_1389	2	10	0.751965	ABC transport system permease
HPG27_1390	2	9	0.173954	outer membrane protein
HPG27_1391	2	10	0.257284	branched-chain-amino-acid aminotransferase
HPG27_1392	1	11	-0.502606	outer membrane protein
HPG27_1393	2	12	-0.755625	DNA polymerase I
HPG27_1394	1	15	-0.291042	type II restriction enzyme
HPG27_1395	3	19	0.198629	restriction enzyme BcgI alpha chain-like
HPG27_1396	3	15	1.006663	hypothetical protein
HPG27_1397	2	14	0.608663	thymidylate kinase
HPG27_1398	1	12	0.869478	lipopolysaccharide core biosynthesis protein
HPG27_1399	1	12	0.905126	aromatic acid decarboxylase
HPG27_1400	2	12	0.478596	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1394	OMPADOMAIN	33	0.002	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 33.0 bits (75), Expect = 0.002 Identities = 19/100 (19%), Positives = 34/100 (34%), Gaps = 9/100 (9%) Query: 212 WQSFKLG-DLFEKVSARFLGKGDKFKATSKSITDTHNIPL-----VYCKKGNNGIMYWGK 265 + F++G D ++ + + +KA +T P+ +Y G M W Sbjct: 69 YVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTAKLGYPITDDLDIY---TRLGGMVWRA 125 Query: 266 KGDFETYNNIISIIYNGVIATGLTYAHRDEVGILAESYFI 305 Y + V A G+ YA E+ E + Sbjct: 126 DTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRLEYQWT 165
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1398	LPSBIOSNTHSS	223	5e-78	Lipopolysaccharide core biosynthesis protein signat...
>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein signature. Length = 166 Score = 223 bits (569), Expect = 5e-78 Identities = 63/147 (42%), Positives = 94/147 (63%) Query: 4 IGIYPGTFDPVTNGHIDIIHRSSELFEKLIVAVAHSSAKNPMFSLKERLKMMQLATKSFK 63 IYPG+FDP+T GH+DII R LF+++ VAV + K PMFS++ERL+ + A Sbjct: 2 NAIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLP 61 Query: 64 NVECVAFEGLLANLAKEYHCKVLVRGLRVVSDFEYELQMGYANKSLNHELETLYFMPTLQ 123 N + +FEGL N A++ ++RGLRV+SDFE ELQM NK+L +LET++ + + Sbjct: 62 NAQVDSFEGLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDLETVFLTTSTE 121 Query: 124 NAFISSSIVRSIIAHKGDASHLVPKEI 150 +F+SSS+V+ + G+ H VP + Sbjct: 122 YSFLSSSLVKEVARFGGNVEHFVPSHV 148

HPG27_1418

HPG27_1451

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_1418	0	11	-4.717391	transaldolase
HPG27_1419	2	12	-3.444708	ribosomal protein L25
HPG27_1420	2	11	-3.624743	peptidyl-tRNA hydrolase
HPG27_1421	2	10	-3.607376	hypothetical protein
HPG27_1422	3	12	-3.311672	type II methylase
HPG27_1423	3	10	-0.700993	type II adenine specific DNA methyltransferase
HPG27_1425	2	11	1.560281	outer membrane protein
HPG27_1426	2	12	1.259043	hypothetical protein
HPG27_1428	1	13	1.471376	hypothetical protein
HPG27_1429	1	13	1.359989	riboflavin biosynthesis protein
HPG27_1430	1	13	1.540153	sodium/glutamate symport carrier
HPG27_1431	3	13	2.715519	hypothetical protein
HPG27_1432	1	12	2.214655	ferrodoxin-like protein
HPG27_1433	-1	10	1.931234	hypothetical protein
HPG27_1434	-1	9	0.709536	dihydroneopterin aldolase
HPG27_1435	-2	9	0.493527	FrpB-like protein
HPG27_1436	-2	9	-2.009969	iron-regulated outer membrane protein
HPG27_1437	0	12	-4.498451	seleno cysteine synthase
HPG27_1438	0	13	-5.325226	transcription termination factor
HPG27_1439	-1	13	-5.437608	hypothetical protein
HPG27_1440	-1	12	-5.119678	hypothetical protein
HPG27_1441	0	11	-4.650681	type II S restriction-modification protein
HPG27_1443	1	12	-3.065603	type III R-M system modification enzyme
HPG27_1444	0	11	-2.584200	type III R-M system modification enzyme
HPG27_1445	1	13	-1.149818	DNA recombinase
HPG27_1446	0	14	-0.902788	hypothetical protein
HPG27_1447	0	13	-0.551352	hypothetical protein
HPG27_1448	1	11	-0.181928	exodeoxyribonuclease
HPG27_1449	2	12	-0.053885	*periplasmic competence protein
HPG27_1450	2	12	-0.918161	chromosomal replication initiator protein
HPG27_1451	2	12	-1.329578	purine nucleoside phosphorylase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1450	HTHFIS	35	4e-04	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 35.2 bits (81), Expect = 4e-04 Identities = 9/51 (17%), Positives = 24/51 (47%), Gaps = 4/51 (7%) Query: 125 TVYEIAKKVAQSDTPPYNPVLFYGGTGLGKTHILNAIGNHALEKHKKVVLV 175 +Y + ++ Q+D ++ G +G GK + A+ ++ ++ V + Sbjct: 148 EIYRVLARLMQTDLT----LMITGESGTGKELVARALHDYGKRRNGPFVAI 194

HPG27_35

HPG27_40

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_35	-3	13	0.213429	competence protein
HPG27_36	-1	13	0.280716	competence protein
HPG27_37	-2	11	1.144389	competence protein
HPG27_38	-2	11	1.065539	mannose-6-phosphate isomerase
HPG27_39	-2	13	1.437229	GDP-D-mannosedehydratase
HPG27_40	-1	15	1.410101	GDP-fucosesynthetase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_35	PF04335	131	5e-40	VirB8 type IV secretion protein
>PF04335#VirB8 type IV secretion protein Length = 227 Score = 131 bits (332), Expect = 5e-40 Identities = 39/203 (19%), Positives = 75/203 (36%), Gaps = 6/203 (2%) Query: 40 QSVFRLERNRLKIAYKLLGLMSFIALILAIVLISVLPLQKTEHHF--VDFLNQDKHYAII 97 + K+A+ + G+ +A + + ++ PL+ E + VD + A Sbjct: 22 RDKLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVDRNTGEASIAAK 81 Query: 98 QRADKSISSNEALARSLIGAYVLNRESINRIDDKSRYELVRLQSSSKVWQRFEDLIKTQN 157 D +I+ +EA+ + + YV RE + ++ V + S+ R+ KT N Sbjct: 82 LHGDATITYDEAVRKYFLATYVRYREGWIAAAREEYFDAVMVMSARPEQDRWSRFYKTDN 141 Query: 158 SIYAQSHLEREVHI-VNIAIYQQDNNPIASVSIAAKLMNENKLVYEKRYKIA-LSYLFDT 215 Q+ L + V I +A V + + + K +A + Y D Sbjct: 142 PQSPQNILANRTDVFVEIKRVSFLGGNVAQVYFTKESVTGSNST--KTDAVATIKYKVDG 199 Query: 216 PDFDYASMPKNPTGFKITRYSIT 238 KNP G+++ Y Sbjct: 200 TPSKEVDRFKNPLGYQVESYRAD 222
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_36	TYPE4SSCAGX	31	0.007	Type IV secretion system CagX conjugation protein si...
>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein signature. Length = 522 Score = 30.9 bits (69), Expect = 0.007 Identities = 23/86 (26%), Positives = 44/86 (51%), Gaps = 13/86 (15%) Query: 181 TNNKPLKEEPLKEEKEETKEKEEETITIGDNTNAMKIVKKDIQKGYRALKSSQRKWYCLG 240 +N + + +E ++EEK++ + + + NA+K + + + Y ++ + Sbjct: 358 SNEQIINKEKIREEKQKIILDQAKALETQYVHNALK--RNPVPRNYNYYQAPE------- 408 Query: 241 ICSKKSKLSLMPKEIFNDKQFTYFKF 266 K+SK +MP EIF+D FTYF F Sbjct: 409 ---KRSK-HIMPSEIFDDGTFTYFGF 430
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_39	NUCEPIMERASE	88	2e-21	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 87.5 bits (217), Expect = 2e-21 Identities = 46/180 (25%), Positives = 72/180 (40%), Gaps = 19/180 (10%) Query: 7 LITGVTGQDGSYLAEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSDHKRRFFLHYGD 66 L+TG G G ++++ LL G++V G+ + + S E L F H D Sbjct: 4 LVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQP---GFQFHKID 60 Query: 67 MTDSSNLIHLIATTKPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILEAMRILGLEN 126 + D + L A+ ++ + V+ S E P A+++ G L ILE R ++ Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ- 119 Query: 127 KTRFYQASTSELYGEVLETPQNENTPF-------NPRSPYAVAKMYAFYITKNYREAYNL 179 AS+S +YG N PF +P S YA K + Y Y L Sbjct: 120 --HLLYASSSSVYGL------NRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGL 171
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_40	NUCEPIMERASE	42	1e-06	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 42.5 bits (100), Expect = 1e-06 Identities = 40/291 (13%), Positives = 92/291 (31%), Gaps = 32/291 (10%) Query: 26 ELYLLDKDNVQAYLKEYKPTGIIHCAGRVGGIVANMNDLSTYMVENLLMGLYLFSSALDL 85 ++ L D++ + + R + ++ + Y NL L + Sbjct: 58 KIDLADREGMTDLFASGHFERVFISPHR-LAVRYSLENPHAYADSNLTGFLNILEGCRHN 116 Query: 86 GVKKAINLASSCAYPKFAPNPLKESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVF 145 ++ + +SS Y P D ++ + YA K + S G+ Sbjct: 117 KIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSL----YAATKKANELMAHTYSHLYGLP 172 Query: 146 YKTLVPCNLYGEFDKFEEKIAHMIPGLIARMHIAKLKNEKNFAMWGDGTARREYLNAKDL 205 L +YG + + P + + K+ ++ G +R++ D+ Sbjct: 173 ATGLRFFTVYGPWGR---------PDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDI 223 Query: 206 ARFIALAYESIAQIPS-----------------VMNVGSGVDYSIEEYYEMVAQVLDYKG 248 A I + I + V N+G+ + +Y + + L + Sbjct: 224 AEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEA 283 Query: 249 VFVKDLSKPVGMQQKLMDISK-QKALKWELEIPLEQGIKEAYEYYLKLLEV 298 +P + + D + + + E ++ G+K +Y +V Sbjct: 284 KKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDFYKV 334

HPG27_223

HPG27_230

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_223	-2	13	1.168561	neutrophil activating protein
HPG27_224	-3	13	1.082274	histidine kinase sensor protein
HPG27_225	-2	12	1.767198	hypothetical protein
HPG27_226	-2	12	2.410721	flagellar basal-body P-ring protein
HPG27_227	-2	10	2.340672	ATP-dependent RNA helicase
HPG27_228	-2	9	2.194471	hypothetical protein
HPG27_229	-2	9	2.160078	hypothetical protein
HPG27_230	-2	9	2.066631	oligopeptide permease ATPase protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_223	HELNAPAPROT	149	3e-49	Helicobacter neutrophil-activating protein A family ...
>HELNAPAPROT#Helicobacter neutrophil-activating protein A family signature. Length = 153 Score = 149 bits (377), Expect = 3e-49 Identities = 39/140 (27%), Positives = 74/140 (52%), Gaps = 1/140 (0%) Query: 5 EILKHLQADAIVLFMKVHNFHWNVKGTDFFNVHKATEEIYEEFADMFDDLAERIAQLGHH 64 L ++ +L+ K+H FHW VKG FF +H+ EE+Y+ A+ D +AER+ +G Sbjct: 15 NSLNTQLSNWFLLYSKLHRFHWYVKGPHFFTLHEKFEELYDHAAETVDTIAERLLAIGGQ 74 Query: 65 PLVTLSEALKLTRVKEETKTSFHSKDIFKEILGDYKHLEKEFKELSNTAEKEGDKVTVTY 124 P+ T+ E + + + + + ++ + ++ DYK + E K + AE+ D T Sbjct: 75 PVATVKEYTEHASITDGGNET-SASEMVQALVNDYKQISSESKFVIGLAEENQDNATADL 133 Query: 125 ADDQLAKLQKSIWMLEAHLA 144 + +++K +WML ++L Sbjct: 134 FVGLIEEVEKQVWMLSSYLG 153
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_224	PF06580	30	0.015	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 29.8 bits (67), Expect = 0.015 Identities = 10/71 (14%), Positives = 25/71 (35%), Gaps = 13/71 (18%) Query: 281 IVLQNFLYNAIDAIEALEESEQ-GQVKIEAFIQNEFIVFTIIDNGKEVENKSALFEPFET 339 +++Q + N I + + Q G++ ++ N + + + G + Sbjct: 258 MLVQTLVENGI--KHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTK------- 308 Query: 340 TKLKGNGLGLA 350 + G GL Sbjct: 309 ---ESTGTGLQ 316
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_226	FLGPRINGFLGI	365	e-128	Flagellar P-ring protein signature.
>FLGPRINGFLGI#Flagellar P-ring protein signature. Length = 373 Score = 365 bits (938), Expect = e-128 Identities = 117/345 (33%), Positives = 190/345 (55%), Gaps = 26/345 (7%) Query: 19 AEKIGDIASVVGVRDNQLIGYGLVIGLNGTGDK-SGSKFTMQSISNMLESVNVKISADDI 77 +I DIAS+ RDNQLIGYGLV+GL GTGD S FT QS+ ML+++ + Sbjct: 28 TSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSMRAMLQNLGITTQGGQS 87 Query: 78 KSKNVAAVMITASLPPFARQGDKIDIHISSIGDAKSIQGGTLVMTPLNAVDGNIYALAQG 137 +KN+AAVM+TA+LPPFA G ++D+ +SS+GDA S++GG L+MT L+ DG IYA+AQG Sbjct: 88 NAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLIMTSLSGADGQIYAVAQG 147 Query: 138 AITSGN-----------SNNLLSANIINGATIEREVSYDLFHKNAMTLSLKNPNFKNAIQ 186 A+ SA + NGA IERE+ + L L+NP+F A++ Sbjct: 148 ALIVNGFSAQGDAATLTQGVTTSARVPNGAIIERELPSKFKDSVNLVLQLRNPDFSTAVR 207 Query: 187 VQNTLNKV----FGNKVAIALDPKTIQITRPERFSMVEFLALVQEIPINYSAKNKIIVDE 242 V + +N +G+ +A D + I + +P + +A ++ + + K++++E Sbjct: 208 VADVVNAFARARYGDPIAEPRDSQEIAVQKPRVADLTRLMAEIENLTVETDTPAKVVINE 267 Query: 243 KSGTIVSGVDIIVHPIVVTSQDITLKITKEP--------LNDSKNTQDLDNNMSLDTAHN 294 ++GTIV G D+ + + V+ +T+++T+ P Q + M++ Sbjct: 268 RTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQPAPFSRGQTAVQPQTDIMAMQEGSK 327 Query: 295 TLSSNGKNITIAGVVKALQKIGVSAKGMVSILQALKKSGAISAEM 339 G ++ +V L IG+ A G+++ILQ +K +GA+ AE+ Sbjct: 328 VAIVEGPDLR--TLVAGLNSIGLKADGIIAILQGIKSAGALQAEL 370
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_227	SECA	30	0.027	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 29.8 bits (67), Expect = 0.027 Identities = 17/63 (26%), Positives = 31/63 (49%), Gaps = 2/63 (3%) Query: 261 IVFTRTKKEADELHQFLASKNYKSTALHGDMDQRDRRASIMAFKKNDADVLVATDVASRG 320 +V T + ++++ + L K L+ + A+I+A A V +AT++A RG Sbjct: 453 LVGTISIEKSELVSNELTKAGIKHNVLNAKFHANE--AAIVAQAGYPAAVTIATNMAGRG 510 Query: 321 LDI 323 DI Sbjct: 511 TDI 513
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_230	HTHFIS	32	0.006	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 31.7 bits (72), Expect = 0.006 Identities = 16/50 (32%), Positives = 21/50 (42%), Gaps = 7/50 (14%) Query: 30 VAIVGESGSGKSSIANLIMRLNPR----FKPHNGEVLFETTNLLKESEEF 75 + I GESG+GK +A + R F N + L ESE F Sbjct: 163 LMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRD---LIESELF 209

HPG27_302

HPG27_309

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_302	1	13	-1.044886	5'-guanylate kinase
HPG27_303	1	13	-1.294420	polyE-rich protein
HPG27_304	-1	14	-2.253231	membrane bound endonuclease
HPG27_305	1	13	-2.163788	outer membrane protein
HPG27_306	4	15	-2.425715	flagellar basal-body L-ring protein
HPG27_307	4	13	-2.029047	CMP-N-acetyl neuraminic acid synthetase
HPG27_308	3	12	-1.220703	CMP-N-acetyl neuraminic acid synthetase
HPG27_309	3	12	-0.882256	flagellar biosynthesis protein G

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_302	PF05272	29	0.011	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 29.3 bits (65), Expect = 0.011 Identities = 9/18 (50%), Positives = 11/18 (61%) Query: 8 LILSGPSGAGKSTLTKYL 25 ++L G G GKSTL L Sbjct: 599 VVLEGTGGIGKSTLINTL 616
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_303	IGASERPTASE	67	1e-13	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 66.6 bits (162), Expect = 1e-13 Identities = 55/266 (20%), Positives = 85/266 (31%), Gaps = 18/266 (6%) Query: 140 ELENLGDLEALAKEEPNNEEQLLPTLDAQEEKEEVKETPQEEKEEVKETPQEEKEEVKET 199 E+E N Q +E + TP E E V E Sbjct: 984 EVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAEN 1043 Query: 200 -PQEEKPKDDETQEGDETPKDEEVSKELETQEKLEIPKEETQKEVKEEIKE--ETQEQEP 256 QE K + Q+ ET ++E+ + K + EV + E ETQ E Sbjct: 1044 SKQESKTVEKNEQDATETTAQ---NREVAKEAKSNVKANTQTNEVAQSGSETKETQTTET 1100 Query: 257 IKEETQENKEEKQEETQDSPSTQELEAMQELVKEIQENSNGQENKEKTQESAEALQETQA 316 + T E +E+ + ET E QE+ K + S QE E Q AE +E Sbjct: 1101 KETATVEKEEKAKVET---------EKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDP 1151 Query: 317 HELEKQEIAETPQELEIPQAQEK---ETPQEETQEKETPKDESMQESAQNLQDKETPQEE 373 K+ ++T + Q ++ Q T+ S+ E+ +N T Sbjct: 1152 TVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTV 1211 Query: 374 TQEDHYESIEDIPEPVMAKAMGEELP 399 E + V + E Sbjct: 1212 NSESSNKPKNRHRRSVRSVPHNVEPA 1237 Score = 63.2 bits (153), Expect = 1e-12 Identities = 35/234 (14%), Positives = 83/234 (35%), Gaps = 9/234 (3%) Query: 148 EALAKEEPNNEEQLLPTLDAQEEKEEVKETPQEEKEEVK-----ETPQEEKEEVKETPQE 202 E +A+ + P ++ + + + QE K K + EV + + Sbjct: 1015 EEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKS 1074 Query: 203 EKPKDDETQEGDETPKDEEVSKELETQEKLEIPKEETQKEVKEEIKEETQEQEPIKEETQ 262 + +T E ++ + + ++ ET+E + KEE K E+ +E + + + Q Sbjct: 1075 NVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPK-Q 1133 Query: 263 ENKEEKQEETQDSPSTQELEAMQELVKEIQENSNGQENKEKTQESAEALQETQAHELEKQ 322 E E Q + + + ++E + ++ ++ ++T + E Sbjct: 1134 EQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGN 1193 Query: 323 EIAETPQELEIPQAQEKETPQEETQEKETPKD-ESMQESAQNLQDKETPQEETQ 375 + E P+ Q E+ K + S++ N++ T + Sbjct: 1194 SVVENPENTTPATTQPTVN--SESSNKPKNRHRRSVRSVPHNVEPATTSSNDRS 1245 Score = 49.3 bits (117), Expect = 3e-08 Identities = 36/186 (19%), Positives = 71/186 (38%), Gaps = 25/186 (13%) Query: 142 ENLGDLEALAKEEPNNEEQLLPTLDAQEEKEEVKET-PQEEKE----EVKETPQEEKEEV 196 E +AKE +N + T + + E KET E KE E +E + E E+ Sbjct: 1060 ETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKT 1119 Query: 197 KETPQ---EEKPKDDETQ----------EGDETPKDEEVSKELETQEKLEIPKEETQKEV 243 +E P+ + PK ++++ E D T +E + T E P +ET V Sbjct: 1120 QEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNV 1179 Query: 244 KEEIKEETQ-------EQEPIKEETQENKEEKQEETQDSPSTQELEAMQELVKEIQENSN 296 ++ + E T + P + E+ + P + +++ + ++ + Sbjct: 1180 EQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATT 1239 Query: 297 GQENKE 302 ++ Sbjct: 1240 SSNDRS 1245
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_306	FLGLRINGFLGH	191	3e-63	Flagellar L-ring protein signature.
>FLGLRINGFLGH#Flagellar L-ring protein signature. Length = 232 Score = 191 bits (486), Expect = 3e-63 Identities = 51/172 (29%), Positives = 84/172 (48%), Gaps = 18/172 (10%) Query: 56 GERPLFADRRAMKPNDLITIIVSEKASANYSSS----KDYKSASGGNSTPPRLTYNGLDE 111 G +PLF DRR D +TI++ E SA+ SSS +D K+ G ++ P L GL Sbjct: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYL--QGLFG 118 Query: 112 RKKQEAQYLDDKNNYNFTKSSNNTNFKGGGSQKKSEDLEIVLSARIIKVLENGNYFIYGN 171 + + + S F G G S L+ + +VL NGN + G Sbjct: 119 NARADVEA------------SGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGE 166 Query: 172 KEVLVDGEKQILKVSGVIRPYDIERNNTIQSKFLADAKIEYTNLGHLSDSNK 223 K++ ++ + ++ SGV+ P I +NT+ S +ADA+IEY G+++++ Sbjct: 167 KQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN 218
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_309	SACTRNSFRASE	28	0.018	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 28.0 bits (62), Expect = 0.018 Identities = 14/49 (28%), Positives = 21/49 (42%), Gaps = 3/49 (6%) Query: 102 KGETILKALECIAFE---EFQLHSLHLEVMENNFKAIAFYEKNHYELEG 147 + + + AL A E E L LE + N A FY K+H+ + Sbjct: 102 RKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGA 150

HPG27_326

HPG27_332

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_326	-2	9	1.124733	CTP synthetase
HPG27_327	-2	9	0.942742	hypothetical protein
HPG27_328	-2	10	0.496607	flagellar basal-body M-ring protein
HPG27_329	-2	10	-0.094640	flagellar motor switch protein
HPG27_330	-2	10	-1.930698	flagellar export protein
HPG27_331	-2	11	-1.929045	1-deoxyxylulose-5-phosphate synthase
HPG27_332	-1	14	-2.784541	GTP-binding membrane protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_326	ACETATEKNASE	29	0.050	Acetate kinase family signature.
>ACETATEKNASE#Acetate kinase family signature. Length = 400 Score = 29.0 bits (65), Expect = 0.050 Identities = 14/38 (36%), Positives = 18/38 (47%), Gaps = 5/38 (13%) Query: 340 LEGVDAILVPGGFGERGIEGKICAIQRARLEKLPFLGI 377 + GVD I+ G GE G I+ L+ L FLG Sbjct: 320 MGGVDVIVFTAGIGENG-----PEIREFILDGLEFLGF 352
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_328	FLGMRINGFLIF	559	0.0	Flagellar M-ring protein signature.
>FLGMRINGFLIF#Flagellar M-ring protein signature. Length = 559 Score = 559 bits (1441), Expect = 0.0 Identities = 177/582 (30%), Positives = 293/582 (50%), Gaps = 66/582 (11%) Query: 11 VDFFIKLNKKQKIALIAAGVLITALLVFLLLYPFKEKDYTQGGYGVLFEGLDPSDNALIL 70 +++ +L +I LI AG A++V ++L+ K DY LF L D I+ Sbjct: 13 LEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWA-KTPDYR-----TLFSNLSDQDGGAIV 66 Query: 71 QHLQQNQIPYKVSRDD-TILIPKDKVYEERITLASQGIPKTSKVGFEIFDTKDFGATDFD 129 L Q IPY+ + I +P DKV+E R+ LA QG+PK VGFE+ D + FG + F Sbjct: 67 AQLTQMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFS 126 Query: 130 QNIKLIRAIEGELSRTIESLNPILKANVHIAIPKDSVFVAKEVPPSASVMLKLKPDMKLS 189 + + RA+EGEL+RTIE+L P+ A VH+A+PK S+FV ++ PSASV + L+P L Sbjct: 127 EQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALD 186 Query: 190 PTQILGIKNLIAAAVPKLTIENVKIVNENGESIGEGDILENSKELALEQLHYKQNFENIL 249 QI + +L+++AV L NV +V+++G + + + + ++L QL + + E+ + Sbjct: 187 EGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNT--SGRDLNDAQLKFANDVESRI 244 Query: 250 ENKIVNILAPIVGGKNKVVARVNAEFDFSQKKSTKETFDPNN-----VVRSEQNLEEKKE 304 + +I IL+PIVG N V A+V A+ DF+ K+ T+E + PN +RS Q ++ Sbjct: 245 QRRIEAILSPIVGNGN-VHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQV 303 Query: 305 GTSKKQVGGVPGVVSN-IGPVQGLKDNKEPEKYEKSQN---------------------- 341 G GGVPG +SN P P + +QN Sbjct: 304 GAGYP--GGVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNE 361 Query: 342 TTNYEVGKTISEIKGEFGTLVRLNAAVVVDGKYKIALKDGANTLEYEPLSDESLQKINAL 401 T+NYEV +TI K G + RL+ AVVV+ K L DG + PL+ + +++I L Sbjct: 362 TSNYEVDRTIRHTKMNVGDIERLSVAVVVNYK---TLADG----KPLPLTADQMKQIEDL 414 Query: 402 VKQAIGYNQNRGDDVAVSNFEFNPMAPMIDNATLSEKIMHKTQKILGSFTPLIKYVLVFI 461 ++A+G++ RGD + V N F+ + T E + Q + +++LV + Sbjct: 415 TREAMGFSDKRGDTLNVVNSPFSAVDN-----TGGELPFWQQQSFIDQLLAAGRWLLVLV 469 Query: 462 VLFIFYKKVIVPFSERMLEVVPDEDKEVKSMFEEMDEEEDELNKLGDLRKKVEDQLGLNA 521 V +I ++K + P R +E ++ + E + E L+K L+++ +Q Sbjct: 470 VAWILWRKAVRPQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQ----- 524 Query: 522 TFSEEEVRYEIILEKIRGTLKERPDEIAMLFKLLIKDEISSD 563 + E++ ++IR E D + L+I+ +S+D Sbjct: 525 -----RLGAEVMSQRIR----EMSDNDPRVVALVIRQWMSND 557
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_329	FLGMOTORFLIG	350	e-122	Flagellar motor switch protein FliG signature.
>FLGMOTORFLIG#Flagellar motor switch protein FliG signature. Length = 344 Score = 350 bits (900), Expect = e-122 Identities = 122/338 (36%), Positives = 209/338 (61%), Gaps = 4/338 (1%) Query: 8 KQKAQLDELSMSEKIAILLIQVGEDTTGEILRHLDIDSITEISKQIVQLNGTDKQIGAAV 67 K+ + L+ +K AILL+ +G + + ++ ++L + I ++ +I +L ++ V Sbjct: 7 KEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKDNV 66 Query: 68 LEEFFAIFQSNQYINTGGLEYARELLTRTLGSEEARKVMDKLTKSLQTQKNFAYLGKIKP 127 L EF + + ++I GG++YARELL ++LG+++A +++ L +LQ+ + F ++ + P Sbjct: 67 LLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQS-RPFEFVRRADP 125 Query: 128 QQLADFIINEHPQTIALILAHMEAPNAAETLSYFPDEMKAEISIRMANLGEISPQVVKRV 187 + +FI EHPQTIALIL++++ A+ LS P E++ ++ R+A + SP+VV+ V Sbjct: 126 ANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEVVREV 185 Query: 188 STVLENKLESLTSYK-IEVGGLRAVAEIFNRLGQKSAKTTLARIESVDNKLAGAIKEMMF 246 VLE KL SL+S GG+ V EI N +K+ K + +E D +LA IK+ MF Sbjct: 186 ERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEIKKKMF 245 Query: 247 TFEDIVKLDNFAIREILKVADKKDLSLALKTSTKDLTDKFLNNMSSRAAEQFVEEMQYLG 306 FEDIV LD+ +I+ +L+ D ++L+ ALK+ + +K NMS RAA E+M++LG Sbjct: 246 VFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKEDMEFLG 305 Query: 307 AVKIKDVDVAQRKIIEIVQSLQEKG--VIQTGEEEDVI 342 + KDV+ +Q+KI+ +++ L+E+G VI G EEDV+ Sbjct: 306 PTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVL 343 Score = 31.3 bits (71), Expect = 0.006 Identities = 20/103 (19%), Positives = 41/103 (39%), Gaps = 3/103 (2%) Query: 4 KLTPKQKAQLDELSMSEKIAILLIQVGEDTTGEILRHLDIDSITEISKQIVQLNGTDKQI 63 + P + + IA++L + IL L + T ++++I ++ T ++ Sbjct: 122 RADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEV 181 Query: 64 GAA---VLEEFFAIFQSNQYINTGGLEYARELLTRTLGSEEAR 103 VLE+ A S Y + GG++ E++ E Sbjct: 182 VREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKF 224
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_330	FLGFLIH	38	2e-05	Flagellar assembly protein FliH signature.
>FLGFLIH#Flagellar assembly protein FliH signature. Length = 228 Score = 37.9 bits (87), Expect = 2e-05 Identities = 47/212 (22%), Positives = 95/212 (44%), Gaps = 17/212 (8%) Query: 37 PNPEEPLEKKAIENDLIDCLLKKTDELSSHLVKLQMQFEKAQEES-KALIENAKNDGYKI 95 P E + E +I+ + L L +LQMQ A E+ +A I + G+K Sbjct: 17 PPQAEFVPIVEPEETIIE---EAEPSLEQQLAQLQMQ---AHEQGYQAGIAEGRQQGHKQ 70 Query: 96 GFKEGEEKMRNELTHSVNEEKNQLLYAITALDEKMKKSQDHLMALE----KELSAIAIDI 151 G++EG + L + E K+Q + + + + Q L AL+ L +A++ Sbjct: 71 GYQEG---LAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIASRLMQMALEA 127 Query: 152 AKEVILKEVEDNSQKVALALAEELLKNVLDATDIHLKVNPLDYPYLNERLQNASKI---K 208 A++VI + ++ + + + L + L + L+V+P D +++ L + + Sbjct: 128 ARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLGATLSLHGWR 187 Query: 209 LESNEAISKGGVMITSSNGSLDGNLMERFKTL 240 L + + GG +++ G LD ++ R++ L Sbjct: 188 LRGDPTLHPGGCKVSADEGDLDASVATRWQEL 219
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_332	TCRTETOQM	116	3e-29	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 116 bits (291), Expect = 3e-29 Identities = 54/162 (33%), Positives = 89/162 (54%), Gaps = 7/162 (4%) Query: 9 NIRNFSIIAHIDHGKSTLADCLISECNAIS---NREMKSQVMDTMDIEKERGITIKAQSV 65 I N ++AH+D GK+TL + L+ AI+ + + + D +E++RGITI+ Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61 Query: 66 RLNYTFKGEDYVLNLIDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTIANVYIAL 125 +F+ E+ +N+IDTPGH+DF EV RSL +GA+L++ A GV+AQT + Sbjct: 62 ----SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALR 117 Query: 126 DNNLEILPVINKIDLPNANVLEVKQDIEDTIGIDCFSANEVS 167 + + INKID ++ V QDI++ + + +V Sbjct: 118 KMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVE 159 Score = 82.2 bits (203), Expect = 2e-18 Identities = 50/215 (23%), Positives = 90/215 (41%), Gaps = 17/215 (7%) Query: 167 SAKAKLGIKDLLEKIITTIPAPSGDFNAPLKALIYDSWFDNYLGALALVRIMDGSINTEQ 226 SAK +GI +L+E I + + + L ++ + LA +R+ G ++ Sbjct: 220 SAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRD 279 Query: 227 EILVMGTGKKHGVLGLYYPNPLKKIPTKSLECGEIGIV---SLGLKSVTDIAVGDTLTDA 283 + + K + +Y + GEI I+ L L SV +GDT Sbjct: 280 SVRISEKEKI-KITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSV----LGDTKLL- 333 Query: 284 KNPTPKPIEGFMPAKPFVFAGLYPIETDRFEDLREALLKLQLNDCALNFEPESSVALGFG 343 P + IE P + + P + + E L +ALL++ +D L + +S+ Sbjct: 334 --PQRERIEN---PLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATH---E 385 Query: 344 FRVGFLGLLHMEVIKERLEREFSLNLIATAPTVVY 378 + FLG + MEV L+ ++ + + PTV+Y Sbjct: 386 IILSFLGKVQMEVTCALLQEKYHVEIEIKEPTVIY 420 Score = 31.0 bits (70), Expect = 0.015 Identities = 15/75 (20%), Positives = 28/75 (37%), Gaps = 2/75 (2%) Query: 405 IKEPFVRATIITPSEFLGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDK 464 + EP++ I P E+L + L + V+L+ +P+ I ++ Sbjct: 535 LLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQ-LKNNEVILSGEIPARCI-QEYRSD 592 Query: 465 LKSCTKGYASFDYEP 479 L T G + E Sbjct: 593 LTFFTNGRSVCLTEL 607

HPG27_562

HPG27_574

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_562	-2	11	0.680268	flagellin A
HPG27_563	-3	11	0.808710	3-methyladenine DNA glycosylase
HPG27_564	-2	11	1.254999	hypothetical protein
HPG27_565	1	9	0.405754	uroporphyrinogen decarboxylase
HPG27_566	1	9	0.032601	outer-membrane protein of the hefABC efflux
HPG27_567	1	9	-0.038856	membrane fusion protein of the hefABC efflux
HPG27_568	2	8	-0.405450	cytoplasmic pump protein of the hefABC efflux
HPG27_569	2	10	-1.259963	hypothetical protein
HPG27_570	1	11	-1.220903	putative vacuolating cytotoxin(VacA)-like
HPG27_571	-1	16	-2.947427	ABC transporter, permease
HPG27_572	-3	12	-1.281288	ABC transporter, permease
HPG27_573	-3	12	-0.943389	ABC transporter. ATP-binding protein
HPG27_574	-2	10	-0.248620	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_562	FLAGELLIN	244	7e-77	Flagellin signature.
>FLAGELLIN#Flagellin signature. Length = 507 Score = 244 bits (624), Expect = 7e-77 Identities = 126/518 (24%), Positives = 209/518 (40%), Gaps = 22/518 (4%) Query: 2 AFQVNTNINAMNAHVQSALTQNALKTSLERLSSGLRINKAADDASGMTVADSLRSQASSL 61 A +NTN ++ +Q++L +++ERLSSGLRIN A DDA+G +A+ S L Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60 Query: 62 GQAIANTNDGMGIIQVADKAMDEQLKILDTVKVKATQAAQDGQTTESRKAIQSDIVRLIQ 121 QA N NDG+ I Q + A++E L V+ + QA + K+IQ +I + ++ Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120 Query: 122 GLDNIGNTTTYNGQALLSGQFTNKEFQVGAYSNQSIKASIGSTTSDKIGQVRI-ATGALI 180 +D + N T +NG +LS + QVGA ++I + +G G Sbjct: 121 EIDRVSNQTQFNGVKVLSQDN-QMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKE 179 Query: 181 TASGDISLTFKQVDGVNDVTLESMKVSSSAGTGIGVLAEVINKNSNRTGVKAYASVITTS 240 GD+ +FK V G + + + K +G V ++ V A +TT Sbjct: 180 ATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTD 239 Query: 241 DVAVQSGSLSNLTLNGIHLGNIADIKKNDSDGRLVAAINAVTSETGVEAYTDQKGRLNLR 300 D N + K A A+ + + + + Sbjct: 240 DAE-----------NNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTID 288 Query: 301 SIDGRGIEIKTDSVSNGPSALTMVNGGQDLTKGSTNYGRLSLTRLDAKSINV------VS 354 + G K + NG V S + +N + Sbjct: 289 TKTGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKT 348 Query: 355 ASDSQHLGFTAIGFGESQVAETTVNLRDVTGNFNANVKSASGANYNAVIASGNQSL---G 411 ++S L ++ TVN + T N + + +G + S Sbjct: 349 KNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINE 408 Query: 412 SGVTTLRGAMVVIDIAESAMKMLDKVRSDLGSVQNQMISTVNNISITQVNVKAAESQIRD 471 + + +SA+ +D VRS LG++QN+ S + N+ T N+ +A S+I D Sbjct: 409 DAAAAKKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIED 468 Query: 472 VDFAEESANFNKNNILAQSGSYAMSQANTVQQNILRLL 509 D+A E +N +K IL Q+G+ ++QAN V QN+L LL Sbjct: 469 ADYATEVSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_563	PF05272	30	0.009	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 30.0 bits (67), Expect = 0.009 Identities = 13/95 (13%), Positives = 26/95 (27%), Gaps = 20/95 (21%) Query: 60 ILENDDEINLKKIAYIEFSKLAECVRPSGFYNQKAKRLIDLSGNILKDFQSFENFKQEVT 119 L + + +A+ E + VR + +KA E+ Sbjct: 458 ALRSAPALA-GCVAFDELREQPVAVRAFPW--RKAPGP-------------LEDADVLRL 501 Query: 120 REWLLDQKGIGKESADAILCYVCAKEVMVVDKYSY 154 +++ G G+ SA + D Sbjct: 502 ADYVETTYGTGEASAQTTEQAINV----AADMNRV 532
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_566	RTXTOXIND	29	0.047	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 29.0 bits (65), Expect = 0.047 Identities = 16/113 (14%), Positives = 41/113 (36%), Gaps = 16/113 (14%) Query: 203 LARMIALQKKLEQIKTDIKRVTKLYDKGLTTIDDL-----QSLKAQGNLSEY--DILDMQ 255 LAR+ + K+ + + L K + + ++A L Y + ++ Sbjct: 220 LARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIE 279 Query: 256 FALEQNRLTLEYLTNLSVKNLKKTTIDAPNLQLRERQD-LVSLREQISALKYQ 307 + + + +T K +D +LR+ D + L +++ + + Sbjct: 280 SEILSAKEEYQLVTQ----LFKNEILD----KLRQTTDNIGLLTLELAKNEER 324
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_567	RTXTOXIND	52	5e-10	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 52.1 bits (125), Expect = 5e-10 Identities = 24/82 (29%), Positives = 37/82 (45%), Gaps = 5/82 (6%) Query: 27 NVKAIQDSKLTLDSTGIVDSIKVTEGSVVKKGDVLLLLYNQDKQAQSDSTEQQLIFAKKQ 86 K I+ IV I V EG V+KGDVLL L +A + T+ L+ A+ + Sbjct: 95 RSKEIKPI-----ENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLE 149 Query: 87 YQRYSKIGGAVDKNTLEGYEFT 108 RY + +++ N L + Sbjct: 150 QTRYQILSRSIELNKLPELKLP 171 Score = 30.6 bits (69), Expect = 0.006 Identities = 23/152 (15%), Positives = 50/152 (32%), Gaps = 25/152 (16%) Query: 70 QAQSDSTEQQLIFAKKQYQR--YSKIGGAVDKNTLEGYEFTYRRLESDYAYSIAVLNKTI 127 +++ S +++ + ++ K+ D L L + A + ++ Sbjct: 279 ESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGL---------LTLELAKNEERQQASV 329 Query: 128 LRAPFDGVIASKNIQVGEGVSANNTVLLRLVSHARKLVIE--FDSKYINAVKVG------ 179 +RAP + + GV L+ +V L + +K I + VG Sbjct: 330 IRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIK 389 Query: 180 -DTYTYSIDGDSNQHEAKITKIYP--TVDENT 208 + + Y+ G K+ I D+ Sbjct: 390 VEAFPYTRYGYL---VGKVKNINLDAIEDQRL 418
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_568	ACRIFLAVINRP	894	0.0	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 894 bits (2311), Expect = 0.0 Identities = 287/1040 (27%), Positives = 517/1040 (49%), Gaps = 42/1040 (4%) Query: 1 MYKTAINRPITTLMFALAIVFFGTMGFKKLSVALFPKIDLPTVVVTTTYPGASAEIIESK 60 M I RPI + A+ ++ G + +L VA +P I P V V+ YPGA A+ ++ Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60 Query: 61 VTDKIEEAVMGIDGIKKVTSTSSKNVSIVV-IEFELEKPNEEALNDVVNKISSVR-FDDS 118 VT IE+ + GID + ++STS S+ + + F+ + A V NK+ Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120 Query: 119 NIKKPSINKFDTDSQAIISLFVSSSSVPAT--TLNDYAKNTIKPMLQKINGVGGVQLNGF 176 +++ I+ + S ++ S + T ++DY + +K L ++NGVG VQL G Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFG- 179 Query: 177 RERQIRIYADPTLMNKYNLTYADLFSTLKAENVEIDGGHIVNS------QRELSILINAN 230 + +RI+ D L+NKY LT D+ + LK +N +I G + + Q SI+ Sbjct: 180 AQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239 Query: 231 SYSVADVEKIQV-----GNHVRLGDIAKIEIGLEEDNTFASFKDKPGVILEIQKIAGANE 285 + + K+ + G+ VRL D+A++E+G E N A KP L I+ GAN Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299 Query: 286 IEIVDRVYEALKHIQAISP-SYEIRPFLDTTSYIRTSIEDVKFDLVLGAILAVLVVFAFL 344 ++ + L +Q P ++ DTT +++ SI +V L +L LV++ FL Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359 Query: 345 RNGTITLVSAISIPISIMGTFALIQWMGFSLNMLTMVALTLAIGIIIDDAIVVIENIHK- 403 +N TL+ I++P+ ++GTFA++ G+S+N LTM + LAIG+++DDAIVV+EN+ + Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419 Query: 404 KLEMGMSKRKASYEGVREIGFALVAISAMLLSVFVPIGNMKGIIGRFFQSFGITVALAIA 463 +E + ++A+ + + +I ALV I+ +L +VF+P+ G G ++ F IT+ A+A Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479 Query: 464 LSYVVVVTIIPMVSSVVVNPRHS-------RFYVWSEPFFKALESRYTKLLQWVLNHKLI 516 LS +V + + P + + ++ P + F+ W F + YT + +L Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539 Query: 517 ISIAVVLVFVGSLFVASKLGMDFMLKEDRGRFLVWLKAKPGVSIDY----MTQKSKIFQK 572 + L+ G + + +L F+ +ED+G FL ++ G + + + Q + + K Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599 Query: 573 AIEKHAEVEFTTLQVGY-GTTQNPFKAKIFVQLKPLKERKKEGELGQFELMSVLRKELKS 631 + + E FT + G QN FV LKP +ER + + + + EL Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNA--GMAFVSLKPWEERNGDENSAEAVIHR-AKMELGK 656 Query: 632 MPEAKGLDTINLSEVALIGGGGDSSPFQTFVFSHSQEAVDKSVENLKKFLLESPELKGKV 691 + + + N+ + G ++ F + + D + + L + + + Sbjct: 657 IRDGFVI-PFNMPAIV---ELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASL 712 Query: 692 ESYHTSTSESQPQLQLKILRQNANKYGVSAQTIGSVVSSAFSGTSQASVFKEDGKEYDMI 751 S + E Q +L++ ++ A GVS I +S+A G + + F + G+ + Sbjct: 713 VSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGG-TYVNDFIDRGRVKKLY 771 Query: 752 IRVPDDKRVSVEDIKRLQVRNKYDKLMFLDALVEITETKSPSSISRYNRQRSVTVLAEPN 811 ++ R+ ED+ +L VR+ +++ A + RYN S+ + E Sbjct: 772 VQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEA- 830 Query: 812 RNAGVSLGEILTQVSKNTKEWLVEGANYRFTGEADNAKESNGEFLVALATAFVLIYMILA 871 G S G+ + + +N L G Y +TG + + S + +A +FV++++ LA Sbjct: 831 -APGTSSGDAMALM-ENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLA 888 Query: 872 ALYESILEPFIIMVTMPLSFSGAFFALGLVHQPLSMFSMIGLILLIGMVGKNATLLIDVA 931 ALYES P +M+ +PL G A L +Q ++ M+GL+ IG+ KNA L+++ A Sbjct: 889 ALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFA 948 Query: 932 NE-ERKKGLNIQEAILFAGKTRLRPILMTTIAMVCGMLPLALASGDGAAMKSPIGIAMSG 990 + K+G + EA L A + RLRPILMT++A + G+LPLA+++G G+ ++ +GI + G Sbjct: 949 KDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMG 1008 Query: 991 GLMISMVLSLLIVPVFYRLL 1010 G++ + +L++ VPVF+ ++ Sbjct: 1009 GMVSATLLAIFFVPVFFVVI 1028
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_570	VACCYTOTOXIN	268	1e-74	Helicobacter pylori vacuolating cytotoxin signature.
>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature. Length = 1291 Score = 268 bits (687), Expect = 1e-74 Identities = 101/394 (25%), Positives = 179/394 (45%), Gaps = 14/394 (3%) Query: 2804 NAVNWLNALFVAKGGNPLFAPYYLQDNPTKHIVTLMKDITSALGMLSKPNLKNNSTDALQ 2863 + L L + + +A + I + T+ L ++ K + L Sbjct: 907 QGRDLLQTLLI-DSHDAGYARTMIDATSANEITKQLNTATTTLNNIASLEHKTSGLQTLS 965 Query: 2864 LNTYTQQMGRLAKLSNFASFDSTDFSERLSSLKNQKFADATPNAMDVILKYSQRDKLKNN 2923 L+ RL LS + F++RL +LK+Q+FA +A +V+ +++ + + N Sbjct: 966 LSNAMILNSRLVNLSRRHTNHIDSFAKRLQALKDQRFAS-LESAAEVLYQFAPKYEKPTN 1024 Query: 2924 LWATGVGGVSFVENGTGTLYGVNVGYDRFIKG---VIVGGYAAYGYSGFYER--ITSSKS 2978 +WA +GG S G +LYG + G D ++ G IVGG+ +YGYS F + +S + Sbjct: 1025 VWANAIGGTSLNSGGNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFSNQANSLNSGA 1084 Query: 2979 DNVDVGLYARAFIKKSELTFSVNETCGANKNQISSADTLLSMINQSYKYSTWTTNAKVNY 3038 +N + G+Y+R F + E F G++++ ++ LL +NQSY Y ++ + +Y Sbjct: 1085 NNTNFGVYSRIFANQHEFDFEAQGALGSDQSSLNFKSALLRDLNQSYNYLAYSAATRASY 1144 Query: 3039 GYDFMFKNKSIILKPQIGLRYYYIGMTGLDGVMHNALYNQFKANADPSKKSVLTIDLALE 3098 GYDF F +++LKP +G+ Y ++G T + S + + +E Sbjct: 1145 GYDFAFFRNALVLKPSVGVSYNHLGSTNFKS----NSNQKVALKNGASSQHLFNASANVE 1200 Query: 3099 NRHYFNTNSYFYAIGGIGRDLLVRSMGDKLVRFIGNNTLSYRKGELYNTFASITAGGEVR 3158 R+Y+ SYFY G+ ++ + V + R NT A + GGE++ Sbjct: 1201 ARYYYGDTSYFYMNAGVLQEFANFGSSNA-VSLNTFKVNATRNP--LNTHARVMMGGELK 1257 Query: 3159 LFKSFYANAGVGARFGLDYKMINITGNIGMRLAF 3192 L K + N G L + + N+GMR +F Sbjct: 1258 LAKEVFLNLGFVYLHNLISNIGHFASNLGMRYSF 1291 Score = 34.2 bits (78), Expect = 0.010 Identities = 15/100 (15%), Positives = 32/100 (32%), Gaps = 5/100 (5%) Query: 699 SYTFDGANNTFNEDKFNGGSFNFNHAEQTDAFNNNSFNGGSFNFNAKQVDFNHNSFNGGV 758 SY+ + E FN + ++A Q +N + G+ + + N + G Sbjct: 272 SYSTINTSKVTGEVNFNHLTVGDHNAAQAGIIASNKTHIGTLDLW-QSAGLNIIAPPEGG 330 Query: 759 FNF---NNTPKVSFTDDTFNVNNQFKING-TQTTFTFNKG 794 + + + + + + N TQ N Sbjct: 331 YKDKPNDKPSNTTQNNAKNDKQESSQNNSNTQVINPPNSA 370 Score = 33.5 bits (76), Expect = 0.021 Identities = 53/325 (16%), Positives = 83/325 (25%), Gaps = 69/325 (21%) Query: 205 NSVNLTNTDFGNQTPNGGFNAMGRKITYNGGIVNGGNFGFDNVDSNGTTTISGVTFNNNG 264 N+ +T N G NA + + G S G I+ G Sbjct: 277 NTSKVTGEVNFNHLTVGDHNA-AQAGIIASNKTHIGTLDLWQ--SAGLNIIA----PPEG 329 Query: 265 ALTYKGGNGIGGSITFTNSNINHYKLNLNANSVTFNNSTLGSMPN------------GNI 312 K + + N N+N+ N G Sbjct: 330 GYKDKPNDKPSNTTQNNAKNDKQESSQNNSNTQVINPPNSAQKTEIQPTQVIDGPFAGGK 389 Query: 313 NTIGNAYILNAN------NITFNNLTFNGGWFVFNRSDAHVNFQGTTTINNPTSPFVNMT 366 NT+ N +N N F + +N + + N+T Sbjct: 390 NTVVNINRINTNADGTIRVGGFKASLTTNAAHLHIGKGG-INLSNQAS--GRSLLVENLT 446 Query: 367 GKVTINPNAIFNIQ--NYTPSIGSAYTLFSM----KNGNITYND---------------- 404 G +T++ N Q Y + SA F KNG T+N+ Sbjct: 447 GNITVDGPLRVNNQVGGYALAGSSANFEFKAGTDTKNGTATFNNDISLGRFVNLKVDAHT 506 Query: 405 --------VNNLWNIIRLKN-----------TQATKDNSKNATSNNTHTYYVTYNLGGTL 445 N +N + T +T KN N ++G Sbjct: 507 ANFKGIDTGNGGFNTLDFSGVTNKVNINKLITASTNVAVKNFNINELVVKTNGVSVGEYT 566 Query: 446 YHFRQIFSPDSIVLQSVYYGANNIY 470 + I S I + G +IY Sbjct: 567 HFSEDIGSQSRINTVRLETGTRSIY 591
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_574	LCRVANTIGEN	31	5e-04	Low calcium response V antigen signature.
>LCRVANTIGEN#Low calcium response V antigen signature. Length = 326 Score = 31.2 bits (70), Expect = 5e-04 Identities = 16/33 (48%), Positives = 20/33 (60%) Query: 16 KRKKLLTELAELEAEIKVSSERKSSFNISLSPS 48 R KL ELAEL AE+K+ S ++ N LS S Sbjct: 149 ARSKLREELAELTAELKIYSVIQAEINKHLSSS 181

HPG27_839

HPG27_846

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_839	1	10	2.664699	cysteinyl-tRNA synthetase
HPG27_840	2	10	3.061409	vacuolating cytotoxin A
HPG27_841	1	16	2.346464	iron (III) dicitrate ABC transporter,
HPG27_842	1	18	2.643638	iron (III) dicitrate ABC transporter, permease
HPG27_843	0	12	-0.512004	short-chain oxidoreductase
HPG27_844	1	14	1.734734	acyl coenzyme A thioesterase
HPG27_845	1	16	0.826646	hypothetical protein
HPG27_846	1	15	0.558264	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_839	TYPE4SSCAGX	30	0.020	Type IV secretion system CagX conjugation protein si...
>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein signature. Length = 522 Score = 30.1 bits (67), Expect = 0.020 Identities = 34/161 (21%), Positives = 73/161 (45%), Gaps = 12/161 (7%) Query: 316 KKRLDKIYRLK----QRVSGTLGGINPNFKKEILECMQDDLNVSKALSVLESMLSSTNEK 371 + LD++ RL+ Q + L I KK+ E ++ ++ +S S + Sbjct: 208 ENELDQMERLEDMQEQAQANALKQIEELNKKQAEEAVRQRAKDKISIKTDKSQKSPEDNS 267 Query: 372 LDQNPKNKALKGEIL--ANLKFIEELLGIGFKD--PSAYFQLGVSESEKQEIENKIEE-- 425 ++ +P + A + ++ N + +L I KD SAY + + ++ E+ + IEE Sbjct: 268 IELSPSDSAWRTNLVVRTNKALYQFILRIAQKDNFASAYLTVKLEYPQRHEVSSVIEEEL 327 Query: 426 --RKRAKEQKDFLKADSIREELLNHKIALMDTPQGTIWEKL 464 R+ AK Q++ +K +++ +++ + Q EK+ Sbjct: 328 KKREEAKRQRELIKQENLNTTAYINRVMMASNEQIINKEKI 368
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_840	VACCYTOTOXIN	2045	0.0	Helicobacter pylori vacuolating cytotoxin signature.
>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature. Length = 1291 Score = 2045 bits (5300), Expect = 0.0 Identities = 1179/1296 (90%), Positives = 1232/1296 (95%), Gaps = 7/1296 (0%) Query: 1 MEIQQTHRKMNRPLVSLVLAGALISAIPQESHAAFFTTVIIPAIVGGIATGTAVGTVSGL 60 MEIQQTHRK+NRPLVSL L GAL+S PQ+SHAAFFTTVIIPAIVGGIATG AVGTVSGL Sbjct: 1 MEIQQTHRKINRPLVSLALVGALVSITPQQSHAAFFTTVIIPAIVGGIATGAAVGTVSGL 60 Query: 61 LSWGLKQAEEANKNPDKPDKVWRIQAGKGFNEFPNKEYDLYKSLLSSKIDGGWDWGNAAR 120 L WGLKQAEEANK PDKPDKVWRIQAGKGFNEFPNKEYDLYKSLLSSKIDGGWDWGNAAR Sbjct: 61 LGWGLKQAEEANKTPDKPDKVWRIQAGKGFNEFPNKEYDLYKSLLSSKIDGGWDWGNAAR 120 Query: 121 HYWVKGGQWNKLEVDMKDAVGTYKLSGLRNFTGGDLDVNMQKATLRLGQFNGNSFTSYKD 180 HYWVK GQWNKLEVDM++AVGTY LSGL NFTGGDLDVNMQKATLRLGQFNGNSFTSYKD Sbjct: 121 HYWVKDGQWNKLEVDMQNAVGTYNLSGLINFTGGDLDVNMQKATLRLGQFNGNSFTSYKD 180 Query: 181 AADRTTRVNFNAKNISIDNFVEINNRVGSGAGRKASSTVLTLQASEGITSDKNAEISLYD 240 +ADRTTRV+FNAKNI IDNF+EINNRVGSGAGRKASSTVLTLQASEGITS +NAEISLYD Sbjct: 181 SADRTTRVDFNAKNILIDNFLEINNRVGSGAGRKASSTVLTLQASEGITSRENAEISLYD 240 Query: 241 GATLNLASSSVKLMGNVWMGRLQYVGAYLAPSYSTINTSKVTGEVNFNHLTVGDKNAAQA 300 GATLNLAS+SVKLMGNVWMGRLQYVGAYLAPSYSTINTSKVTGEVNFNHLTVGD NAAQA Sbjct: 241 GATLNLASNSVKLMGNVWMGRLQYVGAYLAPSYSTINTSKVTGEVNFNHLTVGDHNAAQA 300 Query: 301 GIIASNKTHIGTLDLWQSAGLNIIAPPEGGYKDKPNNTPSQSGTKNDKNESAKNDKQESS 360 GIIASNKTHIGTLDLWQSAGLNIIAPPEGGYKDKPN+ PS + N AKNDKQESS Sbjct: 301 GIIASNKTHIGTLDLWQSAGLNIIAPPEGGYKDKPNDKPSNTTQNN-----AKNDKQESS 355 Query: 361 QNNSNTQVINPPNSTQKTEIQPTQVIDGPFAGGKDTVVNINRINTNADGTIRVGGFKASL 420 QNNSNTQVINPPNS QKTEIQPTQVIDGPFAGGK+TVVNINRINTNADGTIRVGGFKASL Sbjct: 356 QNNSNTQVINPPNSAQKTEIQPTQVIDGPFAGGKNTVVNINRINTNADGTIRVGGFKASL 415 Query: 421 TTNAAHLHIGKGGVNLSNQASGRTLLVENLTGNITVDGPLRVNNQVGGYALAGSSANFEF 480 TTNAAHLHIGKGG+NLSNQASGR+LLVENLTGNITVDGPLRVNNQVGGYALAGSSANFEF Sbjct: 416 TTNAAHLHIGKGGINLSNQASGRSLLVENLTGNITVDGPLRVNNQVGGYALAGSSANFEF 475 Query: 481 KAGVDTKNGTATFNNDISLGRFVNLKVDAHTANFKGIDTGNGGFNTLDFSGVTDKVNINK 540 KAG DTKNGTATFNNDISLGRFVNLKVDAHTANFKGIDTGNGGFNTLDFSGVT+KVNINK Sbjct: 476 KAGTDTKNGTATFNNDISLGRFVNLKVDAHTANFKGIDTGNGGFNTLDFSGVTNKVNINK 535 Query: 541 LITASTNVAVKNFNINELIVKTNGISVGEYTHFSEDIGSQSRINTVRLETGTRSIFSGGV 600 LITASTNVAVKNFNINEL+VKTNG+SVGEYTHFSEDIGSQSRINTVRLETGTRSI+SGGV Sbjct: 536 LITASTNVAVKNFNINELVVKTNGVSVGEYTHFSEDIGSQSRINTVRLETGTRSIYSGGV 595 Query: 601 KFKSGEKLVIDEFYYSPWNYFDARNVKNVEITRKFASSTPENPWGTSKLMFNNLTLGQNA 660 KFK GEKLVI++FYY+PWNYFDARN+KNVEIT K A +PWGT+KLMFNNLTLGQNA Sbjct: 596 KFKGGEKLVINDFYYAPWNYFDARNIKNVEITNKLAFGPQGSPWGTAKLMFNNLTLGQNA 655 Query: 661 VMDYSQFSNLTIQGDFINNQGTINYLVRGGKVATLSVGNAAAMMFNNDIDSATGFYKPLI 720 VMDYSQFSNLTIQGDF+NNQGTINYLVRGG+VATL+VGNAAAM F+N++DSATGFY+PL+ Sbjct: 656 VMDYSQFSNLTIQGDFVNNQGTINYLVRGGQVATLNVGNAAAMFFSNNVDSATGFYQPLM 715 Query: 721 KINSAQDLIKNTEHVLLKAKIIGYGNVSTGTNSISNVNLEEQFKERLALYNNNNRMDTCV 780 KINSAQDLIKN EHVLLKAKIIGYGNVS GT+SI+NVNL EQFKERLALYNNNNRMD CV Sbjct: 716 KINSAQDLIKNKEHVLLKAKIIGYGNVSAGTDSIANVNLIEQFKERLALYNNNNRMDICV 775 Query: 781 VRNTDDIKACGMAIGNQSMVNNPDNYKYLIGKAWKNIGISKTANGSKISVYYLGNSTPTE 840 VRNTDDIKACG AIGNQSMVNNP+NYKYL GKAWKNIGISKTANGSKISV+YLGNSTPTE Sbjct: 776 VRNTDDIKACGTAIGNQSMVNNPENYKYLEGKAWKNIGISKTANGSKISVHYLGNSTPTE 835 Query: 841 NGGNTTNLPTNTTNNARSANYALVKNAPFA-HSATPNLVAINQHDFGTIESVFELANRSK 899 NGGNTTNLPTNTTN R A+YAL+KNAPFA +SATPNLVAINQHDFGTIESVFELANRS Sbjct: 836 NGGNTTNLPTNTTNKVRFASYALIKNAPFARYSATPNLVAINQHDFGTIESVFELANRSN 895 Query: 900 DIDTLYTHSGVQGRDLLQTLLIDSHDAGYARQMIDNTSTGEITKQLNAATDALNNIASLE 959 DIDTLY +SG QGRDLLQTLLIDSHDAGYAR MID TS EITKQLN AT LNNIASLE Sbjct: 896 DIDTLYANSGAQGRDLLQTLLIDSHDAGYARTMIDATSANEITKQLNTATTTLNNIASLE 955 Query: 960 HKTSGLQTLSLSNAMILNSRLVNLSRKHTNHIDSFAQRLQALKGQRFASLESAAEVLYQF 1019 HKTSGLQTLSLSNAMILNSRLVNLSR+HTNHIDSFA+RLQALK QRFASLESAAEVLYQF Sbjct: 956 HKTSGLQTLSLSNAMILNSRLVNLSRRHTNHIDSFAKRLQALKDQRFASLESAAEVLYQF 1015 Query: 1020 APKYEKPTNVWANAIGGASLNNGGNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFSN 1079 APKYEKPTNVWANAIGG SLN+GGNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFSN Sbjct: 1016 APKYEKPTNVWANAIGGTSLNSGGNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFSN 1075 Query: 1080 RANSLNSGANNANFGVYSRIFANQHEFDFEAQGALGSDQSSLNFKSALLQDLNQSYHYLA 1139 +ANSLNSGANN NFGVYSRIFANQHEFDFEAQGALGSDQSSLNFKSALL+DLNQSY+YLA Sbjct: 1076 QANSLNSGANNTNFGVYSRIFANQHEFDFEAQGALGSDQSSLNFKSALLRDLNQSYNYLA 1135 Query: 1140 YSAATRASYGYDFAFFRNALVLKPSVGVSYNHLGSTNFKSSS-NQVALKNGSSSQHLFNA 1198 YSAATRASYGYDFAFFRNALVLKPSVGVSYNHLGSTNFKS+S +VALKNG+SSQHLFNA Sbjct: 1136 YSAATRASYGYDFAFFRNALVLKPSVGVSYNHLGSTNFKSNSNQKVALKNGASSQHLFNA 1195 Query: 1199 NANVEARYYYGDTSYFYMNAGVLQEFARFGSNNAASLNTFKVNTARNPLNTHARVMMGGE 1258 +ANVEARYYYGDTSYFYMNAGVLQEFA FGS+NA SLNTFKVN RNPLNTHARVMMGGE Sbjct: 1196 SANVEARYYYGDTSYFYMNAGVLQEFANFGSSNAVSLNTFKVNATRNPLNTHARVMMGGE 1255 Query: 1259 LQLAKEVFLNLGVVYLHNLISNIGHFASNLGMRYSF 1294 L+LAKEVFLNLG VYLHNLISNIGHFASNLGMRYSF Sbjct: 1256 LKLAKEVFLNLGFVYLHNLISNIGHFASNLGMRYSF 1291
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_843	DHBDHDRGNASE	87	3e-22	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 87.0 bits (215), Expect = 3e-22 Identities = 56/235 (23%), Positives = 106/235 (45%), Gaps = 12/235 (5%) Query: 11 KVAVITGASSGIGLECALMLLDQGYKVYALSRHATLCVALNHALC------ECVDIDVSD 64 K+A ITGA+ GIG A L QG + A+ + + +L E DV D Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 65 SNALKEVFSNISAKEDHCDVLINSAGYGVFGSVEDTPIEEVKKQFGVNFFALCEVVQLCL 124 S A+ E+ + I + D+L+N AG G + EE + F VN + + Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128 Query: 125 PLLKNKPYSKIFNLSSIAGRVSMLFLGHYSASKHALEAYSDALRLELKPFNVQVCLIEPG 184 + ++ I + S V + Y++SK A ++ L LEL +N++ ++ PG Sbjct: 129 KYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPG 188 Query: 185 PVKSNWEKTAFSVENFESEDSLYALEVNAAKSFYSGVYQNALSAKA-VAQKIVFL 238 +++ + + ++ EN + + + ++F +G+ L+ + +A ++FL Sbjct: 189 STETDMQWSLWADENGAEQ-----VIKGSLETFKTGIPLKKLAKPSDIADAVLFL 238
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_846	BINARYTOXINA	26	0.041	Clostridial binary toxin A signature.
>BINARYTOXINA#Clostridial binary toxin A signature. Length = 454 Score = 25.8 bits (56), Expect = 0.041 Identities = 21/71 (29%), Positives = 33/71 (46%), Gaps = 1/71 (1%) Query: 10 YTQYSEKQLFNFLNSIKTKQKRALEKLKEIQAQKQ-RIKKALQFKVLHLIENGYTIEEER 68 Y + EK FN + + + +LEK E++ Q ++ K FK + L E G E+ Sbjct: 134 YFESPEKFAFNKEIRTENQNEISLEKFNELKETIQDKLFKQDGFKDVSLYEPGNGDEKPT 193 Query: 69 EILARAKDTKN 79 +L K KN Sbjct: 194 PLLIHLKLPKN 204

HPG27_980

HPG27_987

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_980	3	19	-4.682522	adenine specific DNA methyltransferase
HPG27_981	4	27	-7.434198	hypothetical protein
HPG27_982	3	26	-6.951846	hypothetical protein
HPG27_983	2	21	-4.182791	periplasmic competence protein-like protein
HPG27_984	2	21	-5.218648	hypothetical protein
HPG27_985	-1	18	-2.365095	hypothetical protein
HPG27_986	-1	16	-1.177857	integrase-recombinase protein
HPG27_987	-2	15	0.751441	****putative neuraminyllactose-binding

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_980	FbpA_PF05833	32	0.046	Fibronectin-binding protein
>FbpA_PF05833#Fibronectin-binding protein Length = 577 Score = 31.8 bits (72), Expect = 0.046 Identities = 47/235 (20%), Positives = 90/235 (38%), Gaps = 27/235 (11%) Query: 1238 EQDYEIIKDFMDKVGENNINLNEQTLNEYFIH-HPENILGRLSLEKTRY-SFETNGEQIY 1295 ++ E+ KD ++ N N T N F+ + N++ + +K +Y S E Y Sbjct: 229 KEIVEVCKDLFKEIQSNKFEFNCYTKNNSFVGFYCLNLMSKEDYKKIQYDSSSKLLENFY 288 Query: 1296 KY--ELQALEDKSLDLSQALNQAIEKLPKDVYQYHKTTLKTDALIIDANNERYQEVQKLI 1353 + L+ KS DL + + I + K + T K + + + ++ +L+ Sbjct: 289 YAKDKSDRLKSKSSDLQKIVMNNINRCTKKDKILNNTLKKCE------DKDIFKLYGELL 342 Query: 1354 K----NLERG-ELVKWDDLYFQLEQNNEMGIFLKPTKINSKVQDSRLKAYFKIKDALNDL 1408 L++G ++ + Y E + + I L K S+ S K Y K+K + Sbjct: 343 TANIYALKKGLSHIELANYY--SENYDTVKITLDENKTPSQNVQSYYKKYNKLKKSEEAA 400 Query: 1409 ------TSAELNPLSS---DLELESKRAKLNLVYDGFVKKFGYLNENKNRKDIKQ 1454 ELN L S ++ ++ + ++ GY+ K K K Sbjct: 401 NEQLLQNEEELNYLYSVLTNINNADNYDEIEEIKKELIET-GYIKFKKIYKSKKS 454
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_982	SECA	31	0.017	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 30.6 bits (69), Expect = 0.017 Identities = 31/169 (18%), Positives = 67/169 (39%), Gaps = 26/169 (15%) Query: 72 ELEELQQTITTDKTQQQLLEQDNIDFELQSALQNDLKDLDHLSDNKDKDDEEQAIQKSFE 131 ++ ++ +TI + ++ + + ID + ++ D+ L + + Sbjct: 668 DVSDVSETI---NSIREDVFKATIDAYIPPQSLEEMWDIPGLQERL----KNDFDLDLPI 720 Query: 132 QDLDDLQNDKLNLEIKEFINKQDDKNYQNKEQLNTETKENIRENSKN-----------SH 180 + D + + ++E I Q + YQ KE++ E +R K H Sbjct: 721 AEWLDKEPELHEETLRERILAQSIEVYQRKEEVVGA--EMMRHFEKGVMLQTLDSLWKEH 778 Query: 181 LIPITNLKNFLHNRRENFKVSQQDLPSEKQKKYSDQLFKKELLEYAKHN 229 L + L+ +H R +Q+D P ++ K+ S +F +LE K+ Sbjct: 779 LAAMDYLRQGIHLR----GYAQKD-PKQEYKRESFSMF-AAMLESLKYE 821
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_983	VACCYTOTOXIN	39	6e-05	Helicobacter pylori vacuolating cytotoxin signature.
>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature. Length = 1291 Score = 39.2 bits (91), Expect = 6e-05 Identities = 55/193 (28%), Positives = 75/193 (38%), Gaps = 29/193 (15%) Query: 139 NTAQTNATNDPMYANTPFSNGSDSSAYDNNPNSPNDNAIN--GKDGANGGNGYGIN-GND 195 N+AQ + PF+ G ++ N N+ D I G + N ++ G Sbjct: 368 NSAQKTEIQPTQVIDGPFAGGKNTVVNINRINTNADGTIRVGGFKASLTTNAAHLHIGKG 427 Query: 196 GINGSNGANGNNRNNSNNNAIGSGIDTDGVLGVDGVNGSNSSSGGSVGGYENNFT----- 250 GIN SN A+G R+ N G I DG L V+ G + +G S NF Sbjct: 428 GINLSNQASG--RSLLVENLTG-NITVDGPLRVNNQVGGYALAGSS-----ANFEFKAGT 479 Query: 251 ---NHGSTNNNTGEYDNFNN-------NSSSGGGLGNGGFFPIPFGNGGTN--NSNNPTN 298 N +T NN F N + G GNGGF + F +G TN N N Sbjct: 480 DTKNGTATFNNDISLGRFVNLKVDAHTANFKGIDTGNGGFNTLDF-SGVTNKVNINKLIT 538 Query: 299 SPTNGSSSNSATN 311 + TN + N N Sbjct: 539 ASTNVAVKNFNIN 551
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_987	PF05211	87	8e-23	Neuraminyllactose-binding hemagglutinin
>PF05211#Neuraminyllactose-binding hemagglutinin Length = 260 Score = 87.4 bits (216), Expect = 8e-23 Identities = 48/220 (21%), Positives = 109/220 (49%), Gaps = 20/220 (9%) Query: 45 HYPIKGKQEPKNGHLVVLIDPKIEANKVIPENYQKEFEKSLFLQLSSFLERKGYSVSQF- 103 ++P K + + ++L+ P + + I + Y+ +F+ L++ L+ +GY V Sbjct: 44 YHPASEKVQALD-EKILLLRPAFQYSDNIAKEYENKFKNQTTLKVEQILQNQGYKVINVD 102 Query: 104 -KDASEIPQDIKEKALLVLRMDGNVAILEDI-----------VEESDALNEEKVIDMSSG 151 D + K++ L + M+G + + D + S L++ + + + +G Sbjct: 103 SSDKDDFSFAQKKEGYLAVAMNGEIVLRPDPKRTIQKKSEPGLLFSTGLDKMEGVLIPAG 162 Query: 152 YLNLNFVEPKSEDIIHSFGIDVSK--IKAVIERVELRRTNSGGFVPKTFVYKIKETDHDQ 209 ++ + +EP S + + SF +D+S+ I+ + ++SGG V K + +D Sbjct: 163 FVKVTILEPMSGESLDSFTMDLSELDIQEKFLKTT-HSSHSGGLVSTMV--KGTDNSND- 218 Query: 210 AIRKIMNQAYHKVMVHITKELSKKHMEHYEKVSSEMKKRK 249 AI+ +N+ + +M I K+L++K++E Y+K + E+K ++ Sbjct: 219 AIKSALNKIFANIMQEIDKKLTQKNLESYQKDAKELKGKR 258

HPG27_1128

HPG27_1133

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_1128	1	27	-2.814640	sugar efflux transporter protein
HPG27_1129	2	28	-3.346827	alpha-carbonic anhydrase
HPG27_1130	2	25	-3.365316	hypothetical protein
HPG27_1131	1	21	-2.526529	hypothetical protein
HPG27_1132	1	18	-1.862742	hypothetical protein
HPG27_1133	-1	15	-0.615081	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1128	TCRTETB	50	8e-09	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 49.9 bits (119), Expect = 8e-09 Identities = 43/193 (22%), Positives = 85/193 (44%), Gaps = 6/193 (3%) Query: 37 LSDIAKSFEMESATVGLMITAYAWVVSLGSLPLMLLSAKIERKRLLLFLFALFIASHILS 96 L DIA F A+ + TA+ S+G+ LS ++ KRLLLF + ++ Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIG 96 Query: 97 ALAWNFWVLLI-SRIGIAFAHSIFWSITASLVIRVAPRNKKQQALGLLALGSSLAMILGL 155 + +F+ LLI +R + F ++ +V R P+ + +A GL+ ++ +G Sbjct: 97 FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGP 156 Query: 156 PLGRIIGQMLDWRSTFGVIGGVATLIALLMWKLLPPLPSRNAGTLASVPILMKRPLLMGI 215 +G +I + W ++ ++ + T+I + L R G I++ + +GI Sbjct: 157 AIGGMIAHYIHW--SYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIIL---MSVGI 211 Query: 216 YLLVIMVISGHFT 228 ++ S + Sbjct: 212 VFFMLFTTSYSIS 224
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1131	IGASERPTASE	36	2e-04	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 36.2 bits (83), Expect = 2e-04 Identities = 27/146 (18%), Positives = 58/146 (39%), Gaps = 7/146 (4%) Query: 98 QSKKEVAETQKEAENARDRANKSGIELEQEQQKTSNIETNNQIKVEQEQQKTEQEKQKTS 157 + + A+ ++ A+ A+ + E Q + ET ++ ++EK K Sbjct: 1057 DATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTET-KETATVEKEEKAKVE 1115 Query: 158 NIETN------NQIKVEQEQQKTEQEKQKTEQEKQKTSNIETNNQIKVEQEKQKTSNIET 211 +T +Q+ +QEQ +T Q + + +E T NI+ + ET Sbjct: 1116 TEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKET 1175 Query: 212 NNQIKVEQEKQKTINTQKDFIKYAEQ 237 ++ ++ + T+NT ++ E Sbjct: 1176 SSNVEQPVTESTTVNTGNSVVENPEN 1201 Score = 35.4 bits (81), Expect = 4e-04 Identities = 33/240 (13%), Positives = 87/240 (36%), Gaps = 14/240 (5%) Query: 102 EVAETQKEAENARDRANKSGIELEQEQQKTSNIETNNQIKVEQEQQKTEQEKQKTSNIET 161 T+ AEN++ + +E+ +Q + N+ ++ + + Q T+ + Sbjct: 1033 PSETTETVAENSKQESK----TVEKNEQDATETTAQNREVAKEAKSNVKANTQ-TNEVAQ 1087 Query: 162 NNQIKVEQEQQKTEQEKQKTEQEKQKTSNIETNNQIKVEQEKQKTSNIETNNQIKVEQEK 221 + E + +T++ ++EK K +T ++ + TS + + + Sbjct: 1088 SGSETKETQTTETKETATVEKEEKAKVETEKT------QEVPKVTSQVSPKQEQSETVQP 1141 Query: 222 QKTINTQKDFIKYAEQNCQENHGQFFIKKGGIKAGIGIEVEAECKTPKPTKTNQTPIQPK 281 Q + D ++ + + ++ + +E T T + Sbjct: 1142 QAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPEN 1201 Query: 282 HLPNSKQPRSQRGSKAQELIAYLQKELESLPYSQKAIAKQVDFYKPSSIAYLELDSRDFN 341 P + QP S + + ++ + S+P++ + + S++A +L S + N Sbjct: 1202 TTPATTQPTVNSESSNKPKNRH-RRSVRSVPHNVEPATTSSN--DRSTVALCDLTSTNTN 1258 Score = 35.4 bits (81), Expect = 4e-04 Identities = 36/208 (17%), Positives = 67/208 (32%) Query: 143 EQEQQKTEQEKQKTSNIETNNQIKVEQEQQKTEQEKQKTEQEKQKTSNIETNNQIKVEQE 202 E + E KQ++ +E N Q E Q E K+ K T E +E Sbjct: 1035 ETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKE 1094 Query: 203 KQKTSNIETNNQIKVEQEKQKTINTQKDFIKYAEQNCQENHGQFFIKKGGIKAGIGIEVE 262 Q T ET K E+ K +T TQ+ ++ + ++ + + V Sbjct: 1095 TQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVN 1154 Query: 263 AECKTPKPTKTNQTPIQPKHLPNSKQPRSQRGSKAQELIAYLQKELESLPYSQKAIAKQV 322 + + T T K ++ + + + ++ + P + + Sbjct: 1155 IKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSE 1214 Query: 323 DFYKPSSIAYLELDSRDFNVTEEWQNEN 350 KP + + S NV + N Sbjct: 1215 SSNKPKNRHRRSVRSVPHNVEPATTSSN 1242
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1132	IGASERPTASE	30	0.012	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.4 bits (68), Expect = 0.012 Identities = 31/209 (14%), Positives = 75/209 (35%), Gaps = 13/209 (6%) Query: 96 DDQSKKEVAETQKEAENARDRANKSGIELGQEKQKTSNIETNNQIKVEQEQQKTEQEKQK 155 D + + +AN E+ Q +T +T + ++ ++EK K Sbjct: 1057 DATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTT---ETKETATVEKEEKAK 1113 Query: 156 TEQEK-----QKTSNIETNNQIKVEQEKQKTINTQKDFIKYAEQNCQEKHNQFFIKKAGI 210 E EK + TS + + + Q + D ++ + + ++ Sbjct: 1114 VETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAK 1173 Query: 211 KGGIGIEVEAECKTHKPAKTNQTPIQPKH-LPNSKQPRSQRGSKAQELIAYLQKELESLP 269 + +E + ++ N P++ P + QP S + + ++ + S+P Sbjct: 1174 ETSSNVE-QPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRH-RRSVRSVP 1231 Query: 270 YSQKAIAKQVDFYKPSSIAYLELDSRDFN 298 ++ + + S++A +L S + N Sbjct: 1232 HNVEPATTSSN--DRSTVALCDLTSTNTN 1258 Score = 29.6 bits (66), Expect = 0.025 Identities = 38/247 (15%), Positives = 75/247 (30%), Gaps = 11/247 (4%) Query: 97 DQSKKEVAETQKEAENARDRANKSGIELGQEKQKTSNIETNNQIKVEQEQQKTEQEKQKT 156 QS E ETQ K + ++ + +Q+ +QEQ +T Q + + Sbjct: 1086 AQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEP 1145 Query: 157 EQEKQKTSNI-----ETNNQIKVEQEKQKTINTQKDFIKYAEQNCQEKHNQFFIKKAGIK 211 +E T NI +TN EQ ++T + + + E N Sbjct: 1146 ARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPV--TESTTVNTGNSVVENPENTT 1203 Query: 212 GGIGIEVEAECKTHKPAKTNQTPIQPK----HLPNSKQPRSQRGSKAQELIAYLQKELES 267 ++KP ++ ++ + + L Sbjct: 1204 PATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTNAVLSD 1263 Query: 268 LPYSQKAIAKQVDFYKPSSIAYLELDSRDFNVTEEWQNENLKIRSKAQAKMLEMRNPQAH 327 + +A V I+ LE+++ K S +Q + ++ Q Sbjct: 1264 ARAKAQFVALNVGKAVSQHISQLEMNNEGQYNVWVSNTSMNKNYSSSQYRRFSSKSTQTQ 1323 Query: 328 LPTSQSL 334 L Q++ Sbjct: 1324 LGWDQTI 1330
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1133	IGASERPTASE	29	0.045	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 28.9 bits (64), Expect = 0.045 Identities = 18/97 (18%), Positives = 39/97 (40%), Gaps = 11/97 (11%) Query: 102 EVAETQKEAENARDRANKSGIELEQEQQKTSNIETNNQIKVEQEKQKT-SNIETNNQIKV 160 T+ AEN++ + +E+ +Q + N+ ++ K +N +TN + Sbjct: 1033 PSETTETVAENSKQESK----TVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQS 1088 Query: 161 EQEKQKTSNIETNNQIKVEQEQQKTEQEKQKTNNTQK 197 E ++T ET ++ ++EK K + Sbjct: 1089 GSETKETQTTET------KETATVEKEEKAKVETEKT 1119

HPG27_1371

HPG27_1379

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
HPG27_1371	0	15	0.598542	60 kDa inner-membrane protein
HPG27_1372	0	11	0.357190	hypothetical protein
HPG27_1373	0	10	0.989189	putative thiophene/furanoxidation protein
HPG27_1374	2	11	1.512913	outer membrane protein
HPG27_1375	1	17	1.023906	hypothetical protein
HPG27_1376	0	17	1.169730	hypothetical protein
HPG27_1377	-2	16	1.122334	hypothetical protein
HPG27_1378	-2	12	2.037911	hypothetical protein
HPG27_1379	-2	10	2.067143	membrane-associated lipoprotein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1371	60KDINNERMP	428	e-147	60kDa inner membrane protein signature.
>60KDINNERMP#60kDa inner membrane protein signature. Length = 548 Score = 428 bits (1102), Expect = e-147 Identities = 168/583 (28%), Positives = 279/583 (47%), Gaps = 85/583 (14%) Query: 10 RLILAIALSFLFIALYSYFFQKPNKT--TTQTTKQETTNNHTATSPNAPNAQNFGTTQTI 67 R +L IAL F+ ++ + Q N QTT+ TT +A P A G ++ Sbjct: 5 RNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAAGSAADQGVP-ASGQGKLISV 63 Query: 68 PQESLLSAISFEHARIEIDSLG-RIKQVYLKDKKYLTPKQKGFLEHVG--HLFSSKEN-- 122 + L + I++ G ++Q L P L L + Sbjct: 64 KTDVL---------DLTINTRGGDVEQALL-------PAYPKELNSTQPFQLLETSPQFI 107 Query: 123 --AQPPL--KELPLLAADKLKPLEVRFLDPTLNNKAFNTPYSASKTTLGPNEQLV--LTQ 176 AQ L ++ P A+ +PL +N A G NE V Sbjct: 108 YQAQSGLTGRDGPDNPANGPRPL-------------YNVEKDAYVLAEGQNELQVPMTYT 154 Query: 177 DLGALIIIKTLTFYDDLHYDLKIAFKSPNN------------------LIPSYVITNGYR 218 D KT Y + + + N L P + Sbjct: 155 DAAGNTFTKTFVLKRG-DYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNF 213 Query: 219 PVADLDSYTFSGVLLENNDKKIEKIE---DKDAKEIKRFSNTLFLSSVDRYFTTLLFTKD 275 + +TF G D+K EK + D + + S +++ + +YF T + Sbjct: 214 AL-----HTFRGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHN 268 Query: 276 PQGFEALIDSEIGTKNPLGFISLKNEA-----------NLHGYIGPKDYRSLKAISPMLT 324 G + +G N + I K++ N ++GP+ + A++P L Sbjct: 269 -DGTNNFYTANLG--NGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLD 325 Query: 325 DVIEYGLITFFAKGVFVLLDYLYQFVGNWGWAIILLTIIVRLILYPLSYKGMVSMQKLKE 384 ++YG + F ++ +F LL +++ FVGNWG++II++T IVR I+YPL+ SM K++ Sbjct: 326 LTVDYGWLWFISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRM 385 Query: 385 LAPKMKELQEKYKGEPQKLQAHMMQLYKKHGANPLGGCLPLILQIPVFFAIYRVLYNAVE 444 L PK++ ++E+ + Q++ MM LYK NPLGGC PL++Q+P+F A+Y +L +VE Sbjct: 386 LQPKIQAMRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVE 445 Query: 445 LKSSEWILWIHDLSIMDPYFILPLLMGASMYWHQSVTPNTMTDPMQAKIFKLLPLLFTIF 504 L+ + + LWIHDLS DPY+ILP+LMG +M++ Q ++P T+TDPMQ KI +P++FT+F Sbjct: 446 LRQAPFALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVF 505 Query: 505 LITFPAGLVLYWTTNNILSVLQQLIINKILENKKRMHAQNKKE 547 + FP+GLVLY+ +N+++++QQ +I + LE K+ +H++ KK+ Sbjct: 506 FLWFPSGLVLYYIVSNLVTIIQQQLIYRGLE-KRGLHSREKKK 547
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1372	IGASERPTASE	29	0.021	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.3 bits (65), Expect = 0.021 Identities = 19/59 (32%), Positives = 26/59 (44%), Gaps = 8/59 (13%) Query: 54 AGVKESVKEVKEESVKETNTKENHQNNIEEKKQKLETETPQEE--IITPKPPKKNPKEE 110 A KE + KET T E +E+K K+ETE QE + + PK+ E Sbjct: 1086 AQSGSETKETQTTETKETATVE------KEEKAKVETEKTQEVPKVTSQVSPKQEQSET 1138
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1373	TCRTETOQM	33	0.003	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 32.5 bits (74), Expect = 0.003 Identities = 32/134 (23%), Positives = 54/134 (40%), Gaps = 25/134 (18%) Query: 170 LSIVGKPNAGKSSLLNAMLLEERA---LVSDIKGTTR-DTIEE-------------VIEL 212 + ++ +AGK++L ++L A L S KGTTR D + Sbjct: 6 IGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW 65 Query: 213 QGHKVRLIDTAGIRESADKIERLGIEKSLKSLENCDIILGVFDLSKPLEKEDFTIIDALN 272 + KV +IDT G + ++ R SL L D + + ++ + + AL Sbjct: 66 ENTKVNIIDTPGHMDFLAEVYR-----SLSVL---DGAILLISAKDGVQAQTRILFHALR 117 Query: 273 RAKKPCIVVLNKND 286 + P I +NK D Sbjct: 118 KMGIPTIFFINKID 131
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1374	TONBPROTEIN	35	7e-04	Gram-negative bacterial tonB protein signature.
>TONBPROTEIN#Gram-negative bacterial tonB protein signature. Length = 239 Score = 35.0 bits (80), Expect = 7e-04 Identities = 13/27 (48%), Positives = 15/27 (55%) Query: 49 EQTIAATQEKPKPKPKPKPKPKPITPQ 75 + EKPKPKPKPKPKP + Sbjct: 82 PKEAPVVIEKPKPKPKPKPKPVKKVQE 108
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1377	BINARYTOXINB	30	0.013	Binary toxin B family signature.
>BINARYTOXINB#Binary toxin B family signature. Length = 764 Score = 30.0 bits (67), Expect = 0.013 Identities = 14/60 (23%), Positives = 21/60 (35%) Query: 155 SKNMGDLLAKAMPIERILKAYSVPVGSLENYEKIYYQNAFKPKVQITFDNNSDAEIKAAL 214 + N D L P + +A + G E + YQ + FD + IK L Sbjct: 536 AVNPSDPLETTKPDMTLKEALKIAFGFNEPNGNLQYQGKDITEFDFNFDQQTSQNIKNQL 595
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
HPG27_1379	LIPOLPP20	293	e-105	LPP20 lipoprotein precursor signature.
>LIPOLPP20#LPP20 lipoprotein precursor signature. Length = 175 Score = 293 bits (752), Expect = e-105 Identities = 174/175 (99%), Positives = 175/175 (100%) Query: 1 MKNQVKKILGMSVIAAMVIVGCSHAPKSGISKSNKAYKEATKGAPDWVVGDLEKVAKYEK 60 MKNQVKKILGMSV+AAMVIVGCSHAPKSGISKSNKAYKEATKGAPDWVVGDLEKVAKYEK Sbjct: 1 MKNQVKKILGMSVVAAMVIVGCSHAPKSGISKSNKAYKEATKGAPDWVVGDLEKVAKYEK 60 Query: 61 YSGVFLGRAEDLITNNDVDYSTNQATAKARANLAANLKSTLQKDLENEKTRTVDASGKRS 120 YSGVFLGRAEDLITNNDVDYSTNQATAKARANLAANLKSTLQKDLENEKTRTVDASGKRS Sbjct: 61 YSGVFLGRAEDLITNNDVDYSTNQATAKARANLAANLKSTLQKDLENEKTRTVDASGKRS 120 Query: 121 ISGTDTEKISQLVDKELIASKMLARYVGKDRVFVLVGLDKQIVDKVREELGMVKK 175 ISGTDTEKISQLVDKELIASKMLARYVGKDRVFVLVGLDKQIVDKVREELGMVKK Sbjct: 121 ISGTDTEKISQLVDKELIASKMLARYVGKDRVFVLVGLDKQIVDKVREELGMVKK 175