:: PredictBias ::

A) Input parameters
Genome	CA382.gbff	Threshold dinucleotide bias	2
Threshold codon bias	4	Threshold %GC bias	3
E-value (RPSBlast)	0.05	Genome (non-pathogenic)

B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start end
Select Organism

C) Potential GIs and PAIs in NC_022048 (download)

S.No

Start

End

Bias

Virulence

Insertion elements

Prediction

L144_RS00335

L144_RS00385

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
L144_RS00335	2	35	-2.255740	Cof-type HAD-IIB family hydrolase
L144_RS00340	3	33	-1.500508	aminopeptidase
L144_RS00345	2	33	-2.715377	divergent PAP2 family protein
L144_RS00350	3	33	-3.278916	hypothetical protein
L144_RS00355	4	33	-3.299458	membrane protein
L144_RS00360	4	27	-3.662518	hypothetical protein
L144_RS00365	3	27	-3.237347	peptide chain release factor 2
L144_RS00370	2	29	-5.153466	hypothetical protein
L144_RS00375	0	20	-4.457408	signal recognition particle-docking protein
L144_RS00380	0	17	-4.217081	hypothetical protein
L144_RS00385	-1	15	-3.478572	ABC transporter ATP-binding protein

L144_RS00860

L144_RS00915

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
L144_RS00860	2	9	2.670566	MoxR family ATPase
L144_RS00865	2	9	2.261221	16S rRNA (guanine(527)-N(7))-methyltransferase
L144_RS00870	2	12	2.682508	tRNA uridine-5-carboxymethylaminomethyl(34)
L144_RS00875	4	19	1.429676	tRNA uridine-5-carboxymethylaminomethyl(34)
L144_RS00880	3	26	1.112964	FlbF protein
L144_RS00885	2	21	1.406133	flagellar hook-associated protein FlgK
L144_RS00890	0	14	0.356771	flagellar hook-associated protein 3
L144_RS00895	0	10	-1.340268	flagellar assembly protein FliW
L144_RS00900	2	14	-1.537618	carbon storage regulator CsrA
L144_RS00905	1	17	0.019703	tRNA
L144_RS00910	2	11	-1.087308	tRNA
L144_RS00915	2	15	-2.958830	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS00860	HTHFIS	41	4e-06	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 41.0 bits (96), Expect = 4e-06 Identities = 36/143 (25%), Positives = 55/143 (38%), Gaps = 15/143 (10%) Query: 33 KEMIDAILMGLLTDGHVLLEGVPGLAKTL---AIQTVSDVLDLEFKRIQ---FTPDLLPS 86 +E+ + + TD +++ G G K L A+ + F I DL+ S Sbjct: 147 QEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIES 206 Query: 87 DLTGNMVYKSA-TGTFKVRKGPVFS----NVILADEINRAPAKVQSALLEAMGERQVT-L 140 +L G K A TG G F + DEI P Q+ LL + + + T + Sbjct: 207 ELFG--HEKGAFTGAQTRSTG-RFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTV 263 Query: 141 GDETHRLPDPFFVLATQNPIEQE 163 G T D V AT ++Q Sbjct: 264 GGRTPIRSDVRIVAATNKDLKQS 286
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS00875	TCRTETOQM	34	0.002	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 33.7 bits (77), Expect = 0.002 Identities = 34/112 (30%), Positives = 48/112 (42%), Gaps = 14/112 (12%) Query: 229 GSVNAGKSSLFNLFLKKDRSIVSSYPGTTRDYIEASFELDGILFNLFDTAGLRDADNFVE 288 GSV+ G + N L++ R I T + I SF+ + N+ DT G D V Sbjct: 34 GSVDKGTTRTDNTLLERQRGI------TIQTGI-TSFQWENTKVNIIDTPGHMDFLAEVY 86 Query: 289 RLGIEKSNSLIKEASLVIYVIDVSSNLTKDDFLFIDSNKSNSKILFVLNKID 340 R S S++ A L+I D T+ LF K +F +NKID Sbjct: 87 R-----SLSVLDGAILLISAKDGVQAQTR--ILFHALRKMGIPTIFFINKID 131
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS00885	FLGHOOKAP1	521	0.0	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 521 bits (1344), Expect = 0.0 Identities = 138/633 (21%), Positives = 260/633 (41%), Gaps = 105/633 (16%) Query: 6 SGIEIGKRSLFAHKDAMNTVGHNLSNATKPGYSRQRVTMKTEIPLYAPQLNRAKKQGQLG 65 S I L A + A+NT +N+S+ GY+RQ M A + G +G Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIM-------AQANSTLGAGGWVG 54 Query: 66 QGIVVQSIDRVKDELLNTRIIEESHRLGYWTSQDKFISILEDVYNEPEDQSIRKRLNDFW 125 G+ V + R D + ++ + T++ + +S ++++ + S+ ++ DF+ Sbjct: 55 NGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLST-STSSLATQMQDFF 113 Query: 126 ESWHDLANQPQGLAERKIILERGKSFCEGIRNRFHSLERIYIMANDEIKI----TTDEAN 181 S L + + A R+ ++ + EG+ N+F + ++ + ++ I + D+ N Sbjct: 114 TSLQTLVSNAEDPAARQALIGKS----EGLVNQFKTTDQYLRDQDKQVNIAIGASVDQIN 169 Query: 182 NYIRNIANLNKQISKSQAMK--DNPNDLMDARDLMVEKLGNIISVSIENKQDPNEFLIHA 239 NY + IA+LN QIS+ + +PN+L+D RD +V +L I+ V + + + A Sbjct: 170 NYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMA 229 Query: 240 EGRHLVQGSIANEF-KLEATNGPTRTRWNIL--WANN---DKAYLKTGKLGSLLNIRDEE 293 G LVQGS A + + ++ P+RT + A N + L TG LG +L R ++ Sbjct: 230 NGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLNTGSLGGILTFRSQD 289 Query: 294 IKNEINELNNIAANIIEIVNEIHEAGRGMDKKNGRSFFSQELKLTDDRGRYDTNGNGQFD 353 + N L +A E N H+AG + G FF+ + N + D Sbjct: 290 LDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIG------KPAVLQNTKNKGD 343 Query: 354 -SVHIFKINSTNEIFPEEKLGFYGTLKFEATNSNEIVEIPYNAPDTVQDVINRINNSNAQ 412 ++ +++ + + K+ F +++ T A +T V N A Sbjct: 344 VAIGATVTDASAVLATDYKISFDNN-QWQVTRL---------ASNTTFTVTPDANGKVAF 393 Query: 413 VTARINSEGKLEIKAVKEQEDENITFKIKHIEDSGSFLTKYTGILNASGPEGAYDYKNID 472 + G AV + +F +K + D I+N ++ Sbjct: 394 DGLELTFTGTP---AVND------SFTLKPVSD---------AIVNM----------DVL 425 Query: 473 TTD--KLAPKSTYSISPLKNPAAWIKVADIIDSDPSKIASGIKNPTNEISIGDNQAALRI 530 TD K+A S + A DSD + + +N ++G ++ Sbjct: 426 ITDEAKIAMAS-------EEDAG--------DSDNRNGQALLDLQSNSKTVGGAKSF--N 468 Query: 531 SSFGNSQIMIGKNLTLNDYFANTASNIAIKGQISEITKESQSQILKDLTDLRMSISGVNK 590 ++ + IG + T N+ +++++ + Q SISGVN Sbjct: 469 DAYASLVSDIGNKTATLKTSSATQGNV-----VTQLSNQQQ------------SISGVNL 511 Query: 591 DEELANMIEFQQAFIAASKFITVSVELIDTVIN 623 DEE N+ FQQ ++A ++ + + + D +IN Sbjct: 512 DEEYGNLQRFQQYYLANAQVLQTANAIFDALIN 544
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS00890	FLAGELLIN	58	4e-11	Flagellin signature.
>FLAGELLIN#Flagellin signature. Length = 507 Score = 57.7 bits (139), Expect = 4e-11 Identities = 37/133 (27%), Positives = 59/133 (44%), Gaps = 5/133 (3%) Query: 10 TYENFKTSSAEQESKITKLLENLYKGGKRIVKLRNDPTGVTHAIRLDNDIFKLNVYIKNI 69 T N S + S I +L G RI ++D G A R ++I L +N Sbjct: 13 TQNNLNKSQSSLSSAIERL-----SSGLRINSAKDDAAGQAIANRFTSNIKGLTQASRNA 67 Query: 70 DTSKSNLRYTEGYLQSLTNILTRAKEIAIQGASGTYESDDKKMISKEVNALLEDVVAIAN 129 + S + TEG L + N L R +E+++Q +GT D K I E+ LE++ ++N Sbjct: 68 NDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDRVSN 127 Query: 130 AKGPDGYSIFSGT 142 +G + S Sbjct: 128 QTQFNGVKVLSQD 140 Score = 31.2 bits (70), Expect = 0.008 Identities = 33/358 (9%), Positives = 87/358 (24%), Gaps = 12/358 (3%) Query: 61 KLNVYIKNIDTSKSNLRYTEGYLQSLTNILTRAKEIAIQGASGTYESDDKKMISKEVNAL 120 + + ++ ID L + TY K + Sbjct: 154 TITIDLQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGA 213 Query: 121 LEDVVAIANAKGPDGYSIFSGTKIDSEAFKVTRENKISKTSKDGAGPQIIKVEYNGNQAE 180 + + +G +A T + T + + + Sbjct: 214 VVTDTTAPTVPDKVYVNAANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGK 273 Query: 181 KKTEVYNDIHMSNNYPGNVIFFLQNQNIISSINTNGFAVKENTKIYIDNIEIGLTAGDTA 240 + + + +S+ I + ++ Sbjct: 274 EGDTFDYK---GVTFTIDTKTGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSS 330 Query: 241 LDIVAKINESSAPVEASIDPVLNSLSIKTTTPHQIWITEEKESNVLQTLGILTKNNDTKL 300 ++ + + LS + + + K+ Sbjct: 331 KNVYTSVVNGQFTFDDKTKNESAKLSDLEANN----AVKGESKITVNGAEYTANAAGDKV 386 Query: 301 PPYNLSSSTEVRSRSIFDALIELRDTLYNNKEELVGSRSLAEIDESLKRLLISVADLGAK 360 + + + + + E + + S ID +L ++ + LGA Sbjct: 387 TLAGKTMFIDKTASGVSTLINEDAAAAKKSTANPLAS-----IDSALSKVDAVRSSLGAI 441 Query: 361 ENRLDRSYERISKEAADMKEDMIQYTDLDVTKAITNLNMASLAYQVSIGISAKIMQTT 418 +NR D + + ++ + D D ++N++ A + Q + A+ Q Sbjct: 442 QNRFDSAITNLGNTVTNLNSARSRIEDADYATEVSNMSKAQILQQAGTSVLAQANQVP 499

L144_RS01380

L144_RS01575

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
L144_RS01380	1	17	3.157351	flagella biosynthesis regulatory protein FliZ
L144_RS01385	1	18	4.113312	flagellar motor switch protein FliN
L144_RS01390	1	16	4.870427	flagellar motor switch protein FliM
L144_RS01395	0	19	4.464363	flagellar basal body-associated protein FliL
L144_RS01400	-2	19	2.527963	flagellar motor protein MotB
L144_RS01405	-2	19	1.911203	motility protein A
L144_RS01410	-1	15	0.372353	flagellar protein FlbD
L144_RS01415	-2	15	1.119653	flagellar hook protein FlgE
L144_RS01420	1	16	-0.322670	flagellar hook assembly protein FlgD
L144_RS01425	5	17	0.685982	flagellar hook-length control protein FliK
L144_RS01430	6	17	2.962283	flagellar protein
L144_RS01435	5	18	3.234829	flagellar protein FlbA
L144_RS01440	5	19	4.228737	flagellar protein export ATPase FliI
L144_RS01445	6	19	3.954428	flagellar assembly protein FliH
L144_RS01450	4	19	3.543925	flagellar motor switch protein FliG
L144_RS01455	2	19	3.427574	flagellar basal body M-ring protein FliF
L144_RS01460	2	19	2.137844	flagellar hook-basal body complex protein FliE
L144_RS01465	1	18	1.512151	flagellar basal body rod protein FlgC
L144_RS01470	1	19	2.796799	flagellar basal body rod protein FlgB
L144_RS01475	0	19	3.869604	HslU--HslV peptidase ATPase subunit
L144_RS01480	-1	19	3.316218	ATP-dependent protease subunit HslV
L144_RS01485	-2	17	2.495167	DNA-protecting protein DprA
L144_RS01490	-1	16	2.598200	hypothetical protein
L144_RS01495	-1	15	2.513109	cell division protein FtsZ
L144_RS01500	0	14	0.773577	cell division protein FtsA
L144_RS01505	1	13	-0.600032	cell division protein FtsQ/DivIB
L144_RS01510	3	15	-0.748834	putative lipid II flippase FtsW
L144_RS01515	1	15	-2.282650	phospho-N-acetylmuramoyl-pentapeptide-
L144_RS01520	-1	16	-3.086143	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
L144_RS01525	0	18	-3.428453	hypothetical protein
L144_RS01530	0	16	-2.846612	16S rRNA (cytosine(1402)-N(4))-methyltransferase
L144_RS01535	2	17	-3.233511	hypothetical protein
L144_RS01540	-1	13	-3.130300	hypothetical protein
L144_RS01545	-1	13	-2.380622	hypothetical protein
L144_RS01550	-1	13	-2.752786	NAD(+)/NADH kinase
L144_RS01555	-1	13	-3.304311	chemotaxis protein CheW
L144_RS01560	0	10	-3.295684	RlmE family RNA methyltransferase
L144_RS01565	0	10	-3.147009	polyprenyl synthetase family protein
L144_RS01570	0	11	-3.176554	hypothetical protein
L144_RS01575	1	15	-3.239931	ABC transporter permease

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01385	FLGMOTORFLIN	103	7e-32	Flagellar motor switch protein FliN signature.
>FLGMOTORFLIN#Flagellar motor switch protein FliN signature. Length = 137 Score = 103 bits (258), Expect = 7e-32 Identities = 40/77 (51%), Positives = 62/77 (80%) Query: 35 NFGLLMDVSMQLTVELGRTERKIKDILGMSEGTIITLDKLAGEPVDILVNGKIVAKGEVV 94 + L+MD+ ++LTVELGRT IK++L +++G+++ LD LAGEP+DIL+NG ++A+GEVV Sbjct: 53 DIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVV 112 Query: 95 VIDENFGVRITEIIKTK 111 V+ + +GVRIT+II Sbjct: 113 VVADKYGVRITDIITPS 129
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01390	FLGMOTORFLIM	458	e-165	Flagellar motor switch protein FliM signature.
>FLGMOTORFLIM#Flagellar motor switch protein FliM signature. Length = 344 Score = 458 bits (1181), Expect = e-165 Identities = 195/345 (56%), Positives = 259/345 (75%), Gaps = 9/345 (2%) Query: 8 LSQDDIDSLLESINSSESLSLDESLSNVISSPTGKKQKVKVYDFKRPDKFSKEQVRTVSS 67 LSQD+ID LL +I+S ++ D + P +K+ +YDF+RPDKFSKEQ+RT+S Sbjct: 5 LSQDEIDQLLTAISSGDASIED-------ARPISDTRKITLYDFRRPDKFSKEQMRTLSL 57 Query: 68 FHEAFARYTTTSLSALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDPLKGSAIF 127 HE FAR TTTSLSA LR MVHVHVASVDQLTYEEFIRSIP P+TLA+I MDPLKG+A+ Sbjct: 58 MHETFARLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVL 117 Query: 128 EVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIEQSVMESVIIRILANMREAWSQVVDLRP 187 EVDP+I F+I+DRLFGG G K + RDLT+IE SVME VI+RILAN+RE+W+QV+DLRP Sbjct: 118 EVDPSITFSIIDRLFGGTGQAAKVQ-RDLTDIENSVMEGVIVRILANVRESWTQVIDLRP 176 Query: 188 RFGHIEVNPQFAQIVPPTEMIILVTLEVKIGKVEGLMNFCLPYITIEPIVSKLSTRYWHS 247 R G IE NPQFAQIVPP+EM++LVTLE K+G+ EG+MNFC+PYITIEPI+SKLS+++W S Sbjct: 177 RLGQIETNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFS 236 Query: 248 LIGVGTTSENLDALREKLENTAMPLVAEIGEVKLKVREILSLDKGDVLNLESSLINKDLT 307 + +T++ + LR+KL M +VAE+G ++L VR+IL L GD++ L + + Sbjct: 237 SVRRSSTTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFV 296 Query: 308 LKVGTKEKFKCRMGLMGNKVSVQITEKIGDIKGFDLLKELTEEVE 352 L +G ++KF C+ G++G K++ QI E+I D +EL+ + E Sbjct: 297 LSIGNRKKFLCQPGVVGKKIAAQILERIESTSQED-FEELSADEE 340
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01400	OMPADOMAIN	55	8e-11	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 54.6 bits (131), Expect = 8e-11 Identities = 28/132 (21%), Positives = 57/132 (43%), Gaps = 21/132 (15%) Query: 124 ISLAADAFFDSASADVKLEENRDSIQKIASFIGFLSPRGYNFKIEGHTDNIDTDVNGPWK 183 +L +D F+ A +K E + ++ ++ S + L P+ + + G+TD I +D Sbjct: 215 FTLKSDVLFNFNKATLK-PEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSD-----A 268 Query: 184 SNWELSAARSVNMLEHILNYLDQSDVKRIENNFEVSGFGGSRPIATDDT---------PE 234 N LS R+ + +++YL + + G G S P+ + + Sbjct: 269 YNQGLSERRA----QSVVDYLISKGIPA--DKISARGMGESNPVTGNTCDNVKQRAALID 322 Query: 235 GRAYNRRIDILI 246 A +RR++I + Sbjct: 323 CLAPDRRVEIEV 334
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01415	FLGHOOKAP1	50	1e-08	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 50.3 bits (120), Expect = 1e-08 Identities = 18/89 (20%), Positives = 33/89 (37%), Gaps = 13/89 (14%) Query: 4 SLYSGVSGLQNHQTRMDVVGNNIANVNTIGFKKGRINFQDMISQSISGASRPTDARGGTN 63 + + +SGL Q ++ NNI++ N G+ + I + T GG Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTT---------IMAQANSTLGAGGW- 52 Query: 64 PKQVGLGMNVASIDTIHTQGAFQSTQKAS 92 VG G+ V+ + + + A Sbjct: 53 ---VGNGVYVSGVQREYDAFITNQLRAAQ 78 Score = 40.7 bits (95), Expect = 1e-05 Identities = 9/48 (18%), Positives = 28/48 (58%) Query: 394 IRSGVLEMANVDLAEQFTDMIVTQRGFQANAKTITTSDQLLQELVRLK 441 + + ++ V+L E++ ++ Q+ + ANA+ + T++ + L+ ++ Sbjct: 499 LSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01425	FLGHOOKFLIK	40	1e-05	Flagellar hook-length control protein signature.
>FLGHOOKFLIK#Flagellar hook-length control protein signature. Length = 375 Score = 39.8 bits (92), Expect = 1e-05 Identities = 24/82 (29%), Positives = 45/82 (54%), Gaps = 5/82 (6%) Query: 281 KLVLKPKELGSIRINLNLDSNNNLLGKIVVDNQNVKMLFDQNMHSLNKMLGESGFNASLN 340 +L L P++LG ++I+L +D N + ++V +Q+V+ + + L L ESG + Sbjct: 260 ELRLHPQDLGEVQISLKVDDNQAQI-QMVSPHQHVRAALEAALPVLRTQLAESGIQLGQS 318 Query: 341 LFLAGENLNSFTGNFKDDSKDQ 362 ++GE SF+G + S+ Q Sbjct: 319 -NISGE---SFSGQQQAASQQQ 336
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01430	TYPE4SSCAGX	29	0.016	Type IV secretion system CagX conjugation protein si...
>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein signature. Length = 522 Score = 29.0 bits (64), Expect = 0.016 Identities = 34/143 (23%), Positives = 66/143 (46%), Gaps = 20/143 (13%) Query: 39 RYFPEFVRTKLLGETSLVFDHNSNIILDEAR--LVKEREAIDIKNQQIEKLKEDLKLKED 96 R + EF++TK L+ D L+E + L KE+EA + + Q+ +K K + + +E Sbjct: 120 RDYQEFLKTK-----KLIVDAPDPKELEEQKKALEKEKEAKE-QAQKAQKDKREKRKEER 173 Query: 97 SLNKLEFELKQKQKDLDLKQKIIDDIINKYNDEEANILQTAVYLMNMPPEDAVKRLEDLN 156 + N+ E + + + N N L + D ++RLED+ Sbjct: 174 AKNRANLE------------NLTNAMSNPQNLSNNKNLSELIKQQRENELDQMERLEDMQ 221 Query: 157 PELAISYMRKIEELSKKEGRLSI 179 + + +++IEEL+KK+ ++ Sbjct: 222 EQAQANALKQIEELNKKQAEEAV 244
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01445	FLGFLIH	47	3e-08	Flagellar assembly protein FliH signature.
>FLGFLIH#Flagellar assembly protein FliH signature. Length = 228 Score = 46.7 bits (110), Expect = 3e-08 Identities = 43/197 (21%), Positives = 96/197 (48%), Gaps = 22/197 (11%) Query: 115 KESIETESNAEIER-LAREYEEKLKTDLDIAIAKGREEGYSKGY--------ESGFEDFD 165 +E+I E+ +E+ LA+ + + IA+GR++G+ +GY E G + Sbjct: 29 EETIIEEAEPSLEQQLAQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAK 88 Query: 166 KVMRKLHAIIASLIAERKGILESSSGQIVSLVMQIAIKVIKRITDSQKDI----VLENVN 221 +HA + L++E + L++ I S +MQ+A++ +++ + +++ + Sbjct: 89 SQQAPIHARMQQLVSEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQ 148 Query: 222 EVLKR---VKDKTQITIRVNLDDLDIVRHKKSDFISRFDIIENLEIIEDPNIGKGGCIIE 278 ++L++ K Q +RV+ DDL V +S + + DP + GGC + Sbjct: 149 QLLQQEPLFSGKPQ--LRVHPDDLQRVDDMLGATLS----LHGWRLRGDPTLHPGGCKVS 202 Query: 279 TNFGEIDARISSQLDKI 295 + G++DA ++++ ++ Sbjct: 203 ADEGDLDASVATRWQEL 219
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01450	FLGMOTORFLIG	427	e-153	Flagellar motor switch protein FliG signature.
>FLGMOTORFLIG#Flagellar motor switch protein FliG signature. Length = 344 Score = 427 bits (1100), Expect = e-153 Identities = 344/344 (100%), Positives = 344/344 (100%) Query: 1 MEEKKEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITS 60 MEEKKEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITS Sbjct: 1 MEEKKEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITS 60 Query: 61 ELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFV 120 ELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFV Sbjct: 61 ELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFV 120 Query: 121 RRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPE 180 RRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPE Sbjct: 121 RRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPE 180 Query: 181 VVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEI 240 VVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEI Sbjct: 181 VVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEI 240 Query: 241 KKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKED 300 KKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKED Sbjct: 241 KKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKED 300 Query: 301 MEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVLV 344 MEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVLV Sbjct: 301 MEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVLV 344
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01455	FLGMRINGFLIF	162	1e-45	Flagellar M-ring protein signature.
>FLGMRINGFLIF#Flagellar M-ring protein signature. Length = 559 Score = 162 bits (412), Expect = 1e-45 Identities = 112/565 (19%), Positives = 222/565 (39%), Gaps = 55/565 (9%) Query: 23 QKIALGLIIFFVILALVFLIGFSTKSQSIALF-GVEIKDQYLLDRISQRLDRENVKYFLS 81 +I L + + +V ++ ++ LF + +D I +L + N+ Y + Sbjct: 23 PRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQD---GGAIVAQLTQMNIPYRFA 79 Query: 82 SDGRIYLDDEKLAKKMRAILVREELVPVHMDPWALFDIDRWTITDFERSINLRRSITRAV 141 + ++R L ++ L + L D +++ I+ F +N +R++ + Sbjct: 80 NGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGEL 139 Query: 142 EQHIVALDDVDAVSVNLVMPEKALFKESQEPVKASVRITPRPGSDIITNRKKVEGLVKLI 201 + I L V + V+L MP+ +LF Q+ ASV +T PG + + ++ +V L+ Sbjct: 140 ARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRAL--DEGQISAVVHLV 197 Query: 202 QYAIEGLESDNIAIVDNSGTILNDFSNLDGIDRIDLAEKERKLKLKYEAMLRGEIDSALS 261 A+ GL N+ +VD SG +L SN G DL + + K E+ ++ I++ LS Sbjct: 198 SSAVAGLPPGNVTLVDQSGHLL-TQSNTSG---RDLNDAQLKFANDVESRIQRRIEAILS 253 Query: 262 KVLSVDRFMIARVNVKLDTSKETTESKEYAPIELQSQDPKASYNTRKVSDSTIISSQTQK 321 ++ A+V +LD + + + Y+P KA+ +R+++ S + + Sbjct: 254 PIVGNGNV-HAQVTAQLDFANKEQTEEHYSP---NGDASKATLRSRQLNISEQVGAGYPG 309 Query: 322 KEYQGQGYSPWGPPGQEGNTPPEYQDLSD-------------ITGKYNESQEIKNVALNE 368 P P TPP Q + + + E N ++ Sbjct: 310 GVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDR 369 Query: 369 KKSTSEKEPARIVGVSLGIFVDGIWNFVYDEKGDFVIENGMRKREYKPMALEEIKNIEDV 428 ++ I +S+ + V+ + + P+ +++K IED+ Sbjct: 370 TIRHTKMNVGDIERLSVAVVVNY---------------KTLADGKPLPLTADQMKQIEDL 414 Query: 429 LQSSFEYKPERGDSITVRNISFDRMNEFREIDENYFASER--FKYFLFIASIVFSLLILV 486 + + + +RGD++ V N F ++ E F ++ L + L++ Sbjct: 415 TREAMGFSDKRGDTLNVVNSPFSAVDN--TGGELPFWQQQSFIDQLLAAGRWLLVLVVAW 472 Query: 487 FTIFFAISRELERRRRLREEELAKQAHLRRQQALMDG-----GDDIGVDDVVGGIREGDE 541 A+ +L RR EE A Q + +Q + D + R G E Sbjct: 473 ILWRKAVRPQLTRR---VEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAE 529 Query: 542 LQSNA-ELLAREKPEDVAKLIRTWL 565 + S ++ P VA +IR W+ Sbjct: 530 VMSQRIREMSDNDPRVVALVIRQWM 554
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01460	FLGHOOKFLIE	86	2e-25	Flagellar hook-basal body complex protein FliE signa...
>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE signature. Length = 103 Score = 85.9 bits (212), Expect = 2e-25 Identities = 15/71 (21%), Positives = 38/71 (53%) Query: 40 TFKDVLINSITDVNKSQLNVSKVTEQAILKPSSIDVHDVVIAMSKANMNLSILKAVVERG 99 +F L ++ ++ +Q E+ L + ++DV+ M KA++++ + V + Sbjct: 32 SFAGQLHAALDRISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKL 91 Query: 100 VKAYQDIINIR 110 V AYQ++++++ Sbjct: 92 VAAYQEVMSMQ 102
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01465	FLGHOOKAP1	48	3e-09	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 47.6 bits (113), Expect = 3e-09 Identities = 21/80 (26%), Positives = 35/80 (43%), Gaps = 15/80 (18%) Query: 5 SSINVASTGLTAQRLRIDVISNNIANVSTSRTPDGGPYRRQRIIFAPRVNNPYWKGPFIP 64 S IN A +GL A + ++ SNNI++ + + Y RQ I A N Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVA------GYTRQTTIMAQ--ANSTLGA---- 49 Query: 65 DYLDNGIGQGVRVASIEKDK 84 +G GV V+ ++++ Sbjct: 50 ---GGWVGNGVYVSGVQREY 66 Score = 44.2 bits (104), Expect = 5e-08 Identities = 11/46 (23%), Positives = 23/46 (50%) Query: 104 KKGYVELPNVNLVEEMVDMISASRAYEANSTVINSSKSMFRSALAI 149 + VNL EE ++ + Y AN+ V+ ++ ++F + + I Sbjct: 500 SNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01475	HTHFIS	31	0.011	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 31.0 bits (70), Expect = 0.011 Identities = 13/70 (18%), Positives = 30/70 (42%), Gaps = 15/70 (21%) Query: 20 KYIIGQDEAKKLVSIALVNRYIRSRLPKEIKDEVMPKNIIMIGSTGIGKTEIAR---RLS 76 ++G+ A + + ++ R +++ L +++ G +G GK +AR Sbjct: 137 MPLVGRSAAMQEI-YRVLARLMQTDLT-----------LMITGESGTGKELVARALHDYG 184 Query: 77 KLIKAPFIKV 86 K PF+ + Sbjct: 185 KRRNGPFVAI 194
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01490	SYCDCHAPRONE	29	0.012	Gram-negative bacterial type III secretion SycD cha...
>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD chaperone signature. Length = 168 Score = 28.7 bits (64), Expect = 0.012 Identities = 16/91 (17%), Positives = 34/91 (37%), Gaps = 2/91 (2%) Query: 69 ARFFNLIGLEFFKLGQYGPAIEYFAKNLEINPNNYLSHFYIGVASYNLAKNLRVKDEVEK 128 +RFF +G +GQY AI ++ ++ F+ + + + Sbjct: 70 SRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFL 129 Query: 129 YI-ILAENSFLKSLSIR-DDFKDSLFAISNM 157 ++A+ + K LS R +++ M Sbjct: 130 AQELIADKTEFKELSTRVSSMLEAIKLKKEM 160
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01500	SHAPEPROTEIN	56	8e-11	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 55.9 bits (135), Expect = 8e-11 Identities = 50/226 (22%), Positives = 87/226 (38%), Gaps = 24/226 (10%) Query: 160 TGSSSSSQNLVR-CVNRAGFAVDEVVLGSLASSYATLSKEEREMGVLFIDMGKGTTDIIL 218 G++ + +R AG ++ +A++ G + +D+G GTT++ + Sbjct: 116 VGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAV 175 Query: 219 YIDGSPYYTGVIPIGVNRVTLDIAQVWK------VPEDVAENIKITAGIAHPSILESQME 272 Y+ + IG +R I + + E AE IK G A+P ++E Sbjct: 176 ISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIE 235 Query: 273 TVIIPNLGTRPPQ--EKSRKELSVIINSRLREIFEMMKAEI------LKRGLYNKINGGI 324 V NL P+ + E+ + L I + + L + + G+ Sbjct: 236 -VRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISER---GM 291 Query: 325 VLTGGGALFPGISNLIEEVFNYPARIGL-PMSINGIGE----EHID 365 VLTGGGAL + L+ E P + P++ G E ID Sbjct: 292 VLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMID 337

L144_RS01730

L144_RS01755

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
L144_RS01730	-1	10	-3.017724	pyruvate kinase
L144_RS01735	0	10	-5.154275	AmmeMemoRadiSam system protein B
L144_RS01740	0	13	-4.644554	50S ribosomal protein L28
L144_RS01745	0	13	-4.984776	hypothetical protein
L144_RS01750	0	13	-5.160375	hypothetical protein
L144_RS01755	0	17	-3.519261	hypothetical protein

L144_RS02000

L144_RS04320

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
L144_RS02000	2	11	-0.201571	dicarboxylate/amino acid:cation symporter
L144_RS02005	0	11	0.900572	proline--tRNA ligase
L144_RS02010	3	12	2.005677	DUF2259 domain-containing protein
L144_RS02015	4	11	2.438633	hypothetical protein
L144_RS02020	3	11	2.411959	DUF3996 domain-containing protein
L144_RS02025	4	10	0.996038	DUF3996 domain-containing protein
L144_RS02030	3	9	0.510417	mannose-6-phosphate isomerase, class I
L144_RS04320	3	12	-0.204841	PTS transporter subunit EIIA

L144_RS03195

L144_RS03265

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
L144_RS03195	2	24	-2.918977	PTS transporter subunit EIIA
L144_RS03200	2	24	-3.713696	1-phosphofructokinase
L144_RS03205	2	21	-3.814880	hypothetical protein
L144_RS03215	2	18	-3.405510	*exodeoxyribonuclease V subunit alpha
L144_RS03220	2	12	-2.605401	exodeoxyribonuclease V subunit beta
L144_RS03225	1	10	-1.562680	nicotinate phosphoribosyltransferase
L144_RS03230	1	9	0.223154	glucose-6-phosphate dehydrogenase
L144_RS03235	2	8	0.158790	Na+/H+ antiporter NhaC family protein
L144_RS03240	3	13	0.719277	Na+/H+ antiporter NhaC family protein
L144_RS03245	3	18	-0.024437	ABC transporter substrate-binding protein
L144_RS03250	4	22	-0.256756	ABC transporter permease
L144_RS03255	1	15	1.728440	ABC transporter permease
L144_RS03260	2	13	2.156090	ABC transporter ATP-binding protein
L144_RS03265	2	14	2.433103	ribosome biogenesis GTPase YlqF

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS03215	MYCMG045	30	0.020	Hypothetical mycoplasma lipoprotein (MG045) signature.
>MYCMG045#Hypothetical mycoplasma lipoprotein (MG045) signature. Length = 483 Score = 30.4 bits (68), Expect = 0.020 Identities = 30/113 (26%), Positives = 58/113 (51%), Gaps = 12/113 (10%) Query: 76 LLAKDIQNTIIFTKDNLEKTNKSYNKLIKILKGLETFGNLETIKNIVLLLK--KNNILME 133 L+ +D+ + I +++ NL+K++ S +K+ + F +++IK I K KNN L+ Sbjct: 84 LIERDLLSPIDWSQFNLKKSSSSSDKVNNASDAKDLF--IDSIKEISQQTKDSKNNELLH 141 Query: 134 FNKLKITTPLILENNIYIYTQKNYREEEE---LIKQIIKRLENHKSELNDNKI 183 + P L+N +++Y + E E+ +IK + HK NDN++ Sbjct: 142 W-----AVPYFLQNLVFVYRGEKISELEQENVSWTDVIKAIVKHKDRFNDNRL 189
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS03250	MYCMG045	35	5e-04	Hypothetical mycoplasma lipoprotein (MG045) signature.
>MYCMG045#Hypothetical mycoplasma lipoprotein (MG045) signature. Length = 483 Score = 34.7 bits (79), Expect = 5e-04 Identities = 25/120 (20%), Positives = 59/120 (49%), Gaps = 4/120 (3%) Query: 1 MKKIFILIAILTTFACTNKDTITLNVFNWAEYIDETLLDQFEKENNIKINYEIFHNNEEM 60 +K F + + + ++ + T + N+ YI LL++ ++++ + + + +NE++ Sbjct: 5 LKYCFFSLFVSLSSILSSCGSTTFVLANFESYISPLLLERVQEKH--PLTFLTYPSNEKL 62 Query: 61 MAKFNNTKNYYDIIVPSEYLIQELIDEGKIEKLDYSKLPNVTKNITQNLTNLEHDPGNLY 120 + F N N Y + V S Y + ELI+ + +D+S+ + + + N D +L+ Sbjct: 63 INGFAN--NTYSVAVASTYAVSELIERDLLSPIDWSQFNLKKSSSSSDKVNNASDAKDLF 120
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS03265	PF05272	31	0.008	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 30.8 bits (69), Expect = 0.008 Identities = 10/20 (50%), Positives = 12/20 (60%) Query: 36 ITLLGPSGCGKTTLIKILGG 55 + L G G GK+TLI L G Sbjct: 599 VVLEGTGGIGKSTLINTLVG 618

L144_RS03525

L144_RS03615

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
L144_RS03525	3	20	2.886183	signal recognition particle protein
L144_RS03530	1	19	1.454997	30S ribosomal protein S16
L144_RS03535	1	20	0.688189	KH domain-containing protein
L144_RS03540	1	20	0.170403	16S rRNA processing protein RimM
L144_RS03545	2	22	0.518622	tRNA (guanosine(37)-N1)-methyltransferase TrmD
L144_RS03550	1	25	0.312323	50S ribosomal protein L19
L144_RS03555	-2	15	-2.422889	hypothetical protein
L144_RS03560	-1	10	-2.418160	pantetheine-phosphate adenylyltransferase
L144_RS03565	0	10	-2.943384	50S ribosomal protein L32
L144_RS03570	-1	9	-2.642078	acyl carrier protein
L144_RS03575	-2	10	-2.496671	ribonuclease III
L144_RS03580	0	9	-1.614831	CCA tRNA nucleotidyltransferase
L144_RS03585	2	15	-0.582854	hypothetical protein
L144_RS03590	3	14	-0.416839	hypothetical protein
L144_RS03605	2	14	0.980444	**endolytic transglycosylase MltG
L144_RS03610	3	14	1.014337	RNA polymerase sigma factor RpoD
L144_RS03615	3	14	0.923863	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS03560	LPSBIOSNTHSS	198	4e-68	Lipopolysaccharide core biosynthesis protein signat...
>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein signature. Length = 166 Score = 198 bits (505), Expect = 4e-68 Identities = 57/157 (36%), Positives = 91/157 (57%), Gaps = 3/157 (1%) Query: 4 AVFPGSFDPITWGHIDLIKRSLAIFDKVIVLVAKNKSKKYFLSDIERFSLTKDVISSLNF 63 A++PGSFDPIT+GH+D+I+R +FD+V V V +N +K+ S ER I+ L Sbjct: 3 AIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHL-- 60 Query: 64 SNVLVDRYSGFIVDYALINSIKFIVRGIRAFNDFDIEFERYLVNNKLNFEIDTIFLPSSA 123 N VD + G V+YA I+RG+R +DF++E + N L +++T+FL +S Sbjct: 61 PNAQVDSFEGLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDLETVFLTTST 120 Query: 124 EHLYVRSDFVKELMLKKDVDLSNFVPELVFNRLKSKF 160 E+ ++ S VKE+ + ++ +FVP V L +F Sbjct: 121 EYSFLSSSLVKEVA-RFGGNVEHFVPSHVAAALYDQF 156

L144_RS04065

L144_RS04160

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
L144_RS04065	2	17	4.075631	transcription termination/antitermination
L144_RS04070	0	15	2.864291	translation initiation factor IF-2
L144_RS04075	-1	15	0.169193	30S ribosome-binding factor RbfA
L144_RS04080	-1	15	0.008010	tRNA pseudouridine(55) synthase TruB
L144_RS04085	0	15	0.565598	30S ribosomal protein S15
L144_RS04090	-1	14	0.176916	polyribonucleotide nucleotidyltransferase
L144_RS04095	1	14	-1.545518	hypothetical protein
L144_RS04100	2	11	-1.163427	YjgP/YjgQ family permease
L144_RS04105	2	11	-0.513569	YjgP/YjgQ family permease
L144_RS04110	4	12	-0.944115	tRNA guanosine(34) transglycosylase Tgt
L144_RS04115	3	12	-1.707995	murein biosynthesis integral membrane protein
L144_RS04120	2	10	-1.501095	HEAT repeat domain-containing protein
L144_RS04125	4	12	-2.084023	bifunctional phosphopantothenoylcysteine
L144_RS04355	2	15	-1.963804	DUF997 family protein
L144_RS04130	0	15	-1.641961	sodium/pantothenate symporter
L144_RS04135	0	14	-1.101863	hypothetical protein
L144_RS04140	0	13	-1.097502	UDP-N-acetylmuramate--L-alanine ligase
L144_RS04145	0	13	-1.287175	YicC family protein
L144_RS04150	0	14	-0.554731	AAA family ATPase
L144_RS04155	-1	12	-0.175543	DNA-directed RNA polymerase subunit omega
L144_RS04160	2	14	-2.100052	tRNA (adenosine(37)-N6)-dimethylallyltransferase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS04070	TCRTETOQM	76	2e-16	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 76.4 bits (188), Expect = 2e-16 Identities = 41/144 (28%), Positives = 60/144 (41%), Gaps = 22/144 (15%) Query: 385 ITIMGHVDHGKTKLLSVL------------------QNIDINQTESGGITQHIGAYTIVY 426 I ++ HVD GKT L L + + GIT G + + Sbjct: 6 IGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW 65 Query: 427 NDREITFLDTPGHEAFTMMRSRGAQVTDIVVLVVSAIDGVMPQTIEAINHAKEANVPIIV 486 + ++ +DTPGH F R V D +L++SA DGV QT + ++ +P I Sbjct: 66 ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIF 125 Query: 487 AINKIDLPDSNPDK----IKHQLS 506 INKID + IK +LS Sbjct: 126 FINKIDQNGIDLSTVYQDIKEKLS 149

L144_RS00860

L144_RS00890

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
L144_RS00860	2	9	2.670566	MoxR family ATPase
L144_RS00865	2	9	2.261221	16S rRNA (guanine(527)-N(7))-methyltransferase
L144_RS00870	2	12	2.682508	tRNA uridine-5-carboxymethylaminomethyl(34)
L144_RS00875	4	19	1.429676	tRNA uridine-5-carboxymethylaminomethyl(34)
L144_RS00880	3	26	1.112964	FlbF protein
L144_RS00885	2	21	1.406133	flagellar hook-associated protein FlgK
L144_RS00890	0	14	0.356771	flagellar hook-associated protein 3

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS00860	HTHFIS	41	4e-06	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 41.0 bits (96), Expect = 4e-06 Identities = 36/143 (25%), Positives = 55/143 (38%), Gaps = 15/143 (10%) Query: 33 KEMIDAILMGLLTDGHVLLEGVPGLAKTL---AIQTVSDVLDLEFKRIQ---FTPDLLPS 86 +E+ + + TD +++ G G K L A+ + F I DL+ S Sbjct: 147 QEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIES 206 Query: 87 DLTGNMVYKSA-TGTFKVRKGPVFS----NVILADEINRAPAKVQSALLEAMGERQVT-L 140 +L G K A TG G F + DEI P Q+ LL + + + T + Sbjct: 207 ELFG--HEKGAFTGAQTRSTG-RFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTV 263 Query: 141 GDETHRLPDPFFVLATQNPIEQE 163 G T D V AT ++Q Sbjct: 264 GGRTPIRSDVRIVAATNKDLKQS 286
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS00875	TCRTETOQM	34	0.002	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 33.7 bits (77), Expect = 0.002 Identities = 34/112 (30%), Positives = 48/112 (42%), Gaps = 14/112 (12%) Query: 229 GSVNAGKSSLFNLFLKKDRSIVSSYPGTTRDYIEASFELDGILFNLFDTAGLRDADNFVE 288 GSV+ G + N L++ R I T + I SF+ + N+ DT G D V Sbjct: 34 GSVDKGTTRTDNTLLERQRGI------TIQTGI-TSFQWENTKVNIIDTPGHMDFLAEVY 86 Query: 289 RLGIEKSNSLIKEASLVIYVIDVSSNLTKDDFLFIDSNKSNSKILFVLNKID 340 R S S++ A L+I D T+ LF K +F +NKID Sbjct: 87 R-----SLSVLDGAILLISAKDGVQAQTR--ILFHALRKMGIPTIFFINKID 131
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS00885	FLGHOOKAP1	521	0.0	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 521 bits (1344), Expect = 0.0 Identities = 138/633 (21%), Positives = 260/633 (41%), Gaps = 105/633 (16%) Query: 6 SGIEIGKRSLFAHKDAMNTVGHNLSNATKPGYSRQRVTMKTEIPLYAPQLNRAKKQGQLG 65 S I L A + A+NT +N+S+ GY+RQ M A + G +G Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIM-------AQANSTLGAGGWVG 54 Query: 66 QGIVVQSIDRVKDELLNTRIIEESHRLGYWTSQDKFISILEDVYNEPEDQSIRKRLNDFW 125 G+ V + R D + ++ + T++ + +S ++++ + S+ ++ DF+ Sbjct: 55 NGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLST-STSSLATQMQDFF 113 Query: 126 ESWHDLANQPQGLAERKIILERGKSFCEGIRNRFHSLERIYIMANDEIKI----TTDEAN 181 S L + + A R+ ++ + EG+ N+F + ++ + ++ I + D+ N Sbjct: 114 TSLQTLVSNAEDPAARQALIGKS----EGLVNQFKTTDQYLRDQDKQVNIAIGASVDQIN 169 Query: 182 NYIRNIANLNKQISKSQAMK--DNPNDLMDARDLMVEKLGNIISVSIENKQDPNEFLIHA 239 NY + IA+LN QIS+ + +PN+L+D RD +V +L I+ V + + + A Sbjct: 170 NYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMA 229 Query: 240 EGRHLVQGSIANEF-KLEATNGPTRTRWNIL--WANN---DKAYLKTGKLGSLLNIRDEE 293 G LVQGS A + + ++ P+RT + A N + L TG LG +L R ++ Sbjct: 230 NGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLNTGSLGGILTFRSQD 289 Query: 294 IKNEINELNNIAANIIEIVNEIHEAGRGMDKKNGRSFFSQELKLTDDRGRYDTNGNGQFD 353 + N L +A E N H+AG + G FF+ + N + D Sbjct: 290 LDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIG------KPAVLQNTKNKGD 343 Query: 354 -SVHIFKINSTNEIFPEEKLGFYGTLKFEATNSNEIVEIPYNAPDTVQDVINRINNSNAQ 412 ++ +++ + + K+ F +++ T A +T V N A Sbjct: 344 VAIGATVTDASAVLATDYKISFDNN-QWQVTRL---------ASNTTFTVTPDANGKVAF 393 Query: 413 VTARINSEGKLEIKAVKEQEDENITFKIKHIEDSGSFLTKYTGILNASGPEGAYDYKNID 472 + G AV + +F +K + D I+N ++ Sbjct: 394 DGLELTFTGTP---AVND------SFTLKPVSD---------AIVNM----------DVL 425 Query: 473 TTD--KLAPKSTYSISPLKNPAAWIKVADIIDSDPSKIASGIKNPTNEISIGDNQAALRI 530 TD K+A S + A DSD + + +N ++G ++ Sbjct: 426 ITDEAKIAMAS-------EEDAG--------DSDNRNGQALLDLQSNSKTVGGAKSF--N 468 Query: 531 SSFGNSQIMIGKNLTLNDYFANTASNIAIKGQISEITKESQSQILKDLTDLRMSISGVNK 590 ++ + IG + T N+ +++++ + Q SISGVN Sbjct: 469 DAYASLVSDIGNKTATLKTSSATQGNV-----VTQLSNQQQ------------SISGVNL 511 Query: 591 DEELANMIEFQQAFIAASKFITVSVELIDTVIN 623 DEE N+ FQQ ++A ++ + + + D +IN Sbjct: 512 DEEYGNLQRFQQYYLANAQVLQTANAIFDALIN 544
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS00890	FLAGELLIN	58	4e-11	Flagellin signature.
>FLAGELLIN#Flagellin signature. Length = 507 Score = 57.7 bits (139), Expect = 4e-11 Identities = 37/133 (27%), Positives = 59/133 (44%), Gaps = 5/133 (3%) Query: 10 TYENFKTSSAEQESKITKLLENLYKGGKRIVKLRNDPTGVTHAIRLDNDIFKLNVYIKNI 69 T N S + S I +L G RI ++D G A R ++I L +N Sbjct: 13 TQNNLNKSQSSLSSAIERL-----SSGLRINSAKDDAAGQAIANRFTSNIKGLTQASRNA 67 Query: 70 DTSKSNLRYTEGYLQSLTNILTRAKEIAIQGASGTYESDDKKMISKEVNALLEDVVAIAN 129 + S + TEG L + N L R +E+++Q +GT D K I E+ LE++ ++N Sbjct: 68 NDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDRVSN 127 Query: 130 AKGPDGYSIFSGT 142 +G + S Sbjct: 128 QTQFNGVKVLSQD 140 Score = 31.2 bits (70), Expect = 0.008 Identities = 33/358 (9%), Positives = 87/358 (24%), Gaps = 12/358 (3%) Query: 61 KLNVYIKNIDTSKSNLRYTEGYLQSLTNILTRAKEIAIQGASGTYESDDKKMISKEVNAL 120 + + ++ ID L + TY K + Sbjct: 154 TITIDLQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGA 213 Query: 121 LEDVVAIANAKGPDGYSIFSGTKIDSEAFKVTRENKISKTSKDGAGPQIIKVEYNGNQAE 180 + + +G +A T + T + + + Sbjct: 214 VVTDTTAPTVPDKVYVNAANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGK 273 Query: 181 KKTEVYNDIHMSNNYPGNVIFFLQNQNIISSINTNGFAVKENTKIYIDNIEIGLTAGDTA 240 + + + +S+ I + ++ Sbjct: 274 EGDTFDYK---GVTFTIDTKTGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSS 330 Query: 241 LDIVAKINESSAPVEASIDPVLNSLSIKTTTPHQIWITEEKESNVLQTLGILTKNNDTKL 300 ++ + + LS + + + K+ Sbjct: 331 KNVYTSVVNGQFTFDDKTKNESAKLSDLEANN----AVKGESKITVNGAEYTANAAGDKV 386 Query: 301 PPYNLSSSTEVRSRSIFDALIELRDTLYNNKEELVGSRSLAEIDESLKRLLISVADLGAK 360 + + + + + E + + S ID +L ++ + LGA Sbjct: 387 TLAGKTMFIDKTASGVSTLINEDAAAAKKSTANPLAS-----IDSALSKVDAVRSSLGAI 441 Query: 361 ENRLDRSYERISKEAADMKEDMIQYTDLDVTKAITNLNMASLAYQVSIGISAKIMQTT 418 +NR D + + ++ + D D ++N++ A + Q + A+ Q Sbjct: 442 QNRFDSAITNLGNTVTNLNSARSRIEDADYATEVSNMSKAQILQQAGTSVLAQANQVP 499

L144_RS01350

L144_RS01500

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
L144_RS01350	-1	18	1.260337	flagellar biosynthesis protein FlhF
L144_RS01355	-1	17	0.952054	flagellar biosynthesis protein FlhA
L144_RS01360	0	18	-0.145149	flagellar biosynthesis protein FlhB
L144_RS01365	-1	17	0.588591	flagellar biosynthetic protein FliR
L144_RS01370	0	17	1.734156	flagellar biosynthesis protein FliQ
L144_RS01375	0	16	2.300564	flagellar type III secretion system pore protein
L144_RS01380	1	17	3.157351	flagella biosynthesis regulatory protein FliZ
L144_RS01385	1	18	4.113312	flagellar motor switch protein FliN
L144_RS01390	1	16	4.870427	flagellar motor switch protein FliM
L144_RS01395	0	19	4.464363	flagellar basal body-associated protein FliL
L144_RS01400	-2	19	2.527963	flagellar motor protein MotB
L144_RS01405	-2	19	1.911203	motility protein A
L144_RS01410	-1	15	0.372353	flagellar protein FlbD
L144_RS01415	-2	15	1.119653	flagellar hook protein FlgE
L144_RS01420	1	16	-0.322670	flagellar hook assembly protein FlgD
L144_RS01425	5	17	0.685982	flagellar hook-length control protein FliK
L144_RS01430	6	17	2.962283	flagellar protein
L144_RS01435	5	18	3.234829	flagellar protein FlbA
L144_RS01440	5	19	4.228737	flagellar protein export ATPase FliI
L144_RS01445	6	19	3.954428	flagellar assembly protein FliH
L144_RS01450	4	19	3.543925	flagellar motor switch protein FliG
L144_RS01455	2	19	3.427574	flagellar basal body M-ring protein FliF
L144_RS01460	2	19	2.137844	flagellar hook-basal body complex protein FliE
L144_RS01465	1	18	1.512151	flagellar basal body rod protein FlgC
L144_RS01470	1	19	2.796799	flagellar basal body rod protein FlgB
L144_RS01475	0	19	3.869604	HslU--HslV peptidase ATPase subunit
L144_RS01480	-1	19	3.316218	ATP-dependent protease subunit HslV
L144_RS01485	-2	17	2.495167	DNA-protecting protein DprA
L144_RS01490	-1	16	2.598200	hypothetical protein
L144_RS01495	-1	15	2.513109	cell division protein FtsZ
L144_RS01500	0	14	0.773577	cell division protein FtsA

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01350	PF05272	31	0.011	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 30.8 bits (69), Expect = 0.011 Identities = 8/23 (34%), Positives = 12/23 (52%) Query: 176 VFILVGPTGVGKTTTIAKLAAIY 198 +L G G+GK+T I L + Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLD 620
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01360	TYPE3IMSPROT	339	e-117	Type III secretion system inner membrane S protein ...
>TYPE3IMSPROT#Type III secretion system inner membrane S protein family signature. Length = 354 Score = 339 bits (871), Expect = e-117 Identities = 101/345 (29%), Positives = 182/345 (52%), Gaps = 9/345 (2%) Query: 25 RTELPTDQKKQKAREEGRVLKSTEINTAVSLLLLFALFFFMLSYFA---LDLIAVFKEQA 81 +TE PT +K + AR++G+V KS E+ + ++ L A+ + Y+ L+ + EQ+ Sbjct: 5 KTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLMLIPAEQS 64 Query: 82 IKLPEVMRMSVYTMGFAYIRSIMGYVVLFFFASLAVNFFVNIIQVGFFITFKSLEPRWDK 141 LP +S + + +L A +A+ +++Q GF I+ ++++P K Sbjct: 65 Y-LPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIA--SHVVQYGFLISGEAIKPDIKK 121 Query: 142 ISFNFSRWAKNSFFSAGAFFNLFKSLLKVVIICLIYYFIIENNIGKISKLSEYTLQSGIS 201 I N AK FS + KS+LKVV++ ++ + II+ N+ + +L ++ Sbjct: 122 I--NPIEGAKR-IFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITP 178 Query: 202 IVLVIAYKICFFSVMFLAIVGVFDYLFQRSQYIESLKMTKEEVKQERKEMEGDPLLRSRI 261 ++ I ++ + ++ + DY F+ QYI+ LKM+K+E+K+E KEMEG P ++S+ Sbjct: 179 LLGQILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKR 238 Query: 262 KERMRVILSTNLRVAIPQADVVITNPEHFAVAIKWDSETMLAPKVLAKGQDEIALTIKKI 321 ++ + I S N+R + ++ VV+ NP H A+ I + P V K D T++KI Sbjct: 239 RQFHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKI 298 Query: 322 ARENNVPLMENKLLARALYANVKVNEEIPREYWEIVSKILVRVYS 366 A E VP+++ LARALY + V+ IP E E +++L + Sbjct: 299 AEEEGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLER 343
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01365	TYPE3IMRPROT	113	2e-32	Type III secretion system inner membrane R protein ...
>TYPE3IMRPROT#Type III secretion system inner membrane R protein family signature. Length = 261 Score = 113 bits (285), Expect = 2e-32 Identities = 46/242 (19%), Positives = 107/242 (44%), Gaps = 4/242 (1%) Query: 16 VLVRIFMFLKFSPFFSTIKI-GYFNFFFSLILSVIVVEKIKIIYPLDNMLSFALILLGEA 74 L+R+ + +P S + +++++ + + + + + Sbjct: 19 PLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPANDVPVFSFFALWLAVQQI 78 Query: 75 ILGLIQAFFVNIIFNVFHLVGFFFSNQIGLAYANIFDVFSEEDSMIISQIFAYLFLLLFL 134 ++G+ F + F G Q+GL++A D S + ++++I L LLLFL Sbjct: 79 LIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFL 138 Query: 135 SSDFLLRFFVIGIHDSVLNIRVEHLVNMRNSGFVKLLLMSFGFLFEKALLISFPILSLLL 194 + + L + + + D+ + + NS L + +F L+++ P+++LLL Sbjct: 139 TFNGHL-WLISLLVDTFHTLPI--GGEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLL 195 Query: 195 LFYLVLGILSKSSPQINLLIISFSTSLFLGLLILYIGFPSLAISSKRVIELSLDSLASFL 254 L LG+L++ +PQ+++ +I F +L +G+ ++ P +A + + + LA + Sbjct: 196 TLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADII 255 Query: 255 KL 256 Sbjct: 256 SE 257
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01370	TYPE3IMQPROT	61	2e-16	Type III secretion system inner membrane Q protein ...
>TYPE3IMQPROT#Type III secretion system inner membrane Q protein family signature. Length = 86 Score = 61.3 bits (149), Expect = 2e-16 Identities = 21/76 (27%), Positives = 43/76 (56%) Query: 6 ILYLIRISIENIIILSAPMLIIALIVGLLISIFQAITSIQDQTLSFIPKIIVILLVIVIF 65 +++ ++ ++ILS I+A I+GLL+ +FQ +T +Q+QTL F K++ + L + + Sbjct: 4 LVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLFLL 63 Query: 66 GPWILNKLMQFTYMIF 81 W L+ + + Sbjct: 64 SGWYGEVLLSYGRQVI 79
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01375	FLGBIOSNFLIP	260	3e-90	Escherichia coli: Flagellar biosynthetic protein Fl...
>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP signature. Length = 245 Score = 260 bits (667), Expect = 3e-90 Identities = 97/213 (45%), Positives = 138/213 (64%), Gaps = 3/213 (1%) Query: 41 GGSEIAFSLQLLILLTIITLSPAFLVLMTSFLRISIVLDFIRRALSLQQSPPTQIVMGLA 100 GG + +Q L+ +T +T PA L++MTSF RI IV +R AL +PP Q+++GLA Sbjct: 34 GGQSWSLPVQTLVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLA 93 Query: 101 LFLTIFTMWPTFNSIYEQAYLPLKESKINFNEFYNKGIAPLRIFMYKQMSDGRHEEIRLF 160 LFLT F M P + IY AY P E KI+ E KG PLR FM +Q R ++ LF Sbjct: 94 LFLTFFIMSPVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQT---READLGLF 150 Query: 161 MSMSNYDRPKNFSEVPTHVLIAAFILHELKVAFKMGILIFLPFIVLDIIVASVLMAMGMI 220 ++N + VP +L+ A++ ELK AF++G IF+PF+++D+++ASVLMA+GM+ Sbjct: 151 ARLANTGPLQGPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMM 210 Query: 221 MLPPVMISLPFKLILFVMVDGWTLITSGLIKSF 253 M+PP I+LPFKL+LFV+VDGW L+ L +SF Sbjct: 211 MVPPATIALPFKLMLFVLVDGWQLLVGSLAQSF 243
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01385	FLGMOTORFLIN	103	7e-32	Flagellar motor switch protein FliN signature.
>FLGMOTORFLIN#Flagellar motor switch protein FliN signature. Length = 137 Score = 103 bits (258), Expect = 7e-32 Identities = 40/77 (51%), Positives = 62/77 (80%) Query: 35 NFGLLMDVSMQLTVELGRTERKIKDILGMSEGTIITLDKLAGEPVDILVNGKIVAKGEVV 94 + L+MD+ ++LTVELGRT IK++L +++G+++ LD LAGEP+DIL+NG ++A+GEVV Sbjct: 53 DIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVV 112 Query: 95 VIDENFGVRITEIIKTK 111 V+ + +GVRIT+II Sbjct: 113 VVADKYGVRITDIITPS 129
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01390	FLGMOTORFLIM	458	e-165	Flagellar motor switch protein FliM signature.
>FLGMOTORFLIM#Flagellar motor switch protein FliM signature. Length = 344 Score = 458 bits (1181), Expect = e-165 Identities = 195/345 (56%), Positives = 259/345 (75%), Gaps = 9/345 (2%) Query: 8 LSQDDIDSLLESINSSESLSLDESLSNVISSPTGKKQKVKVYDFKRPDKFSKEQVRTVSS 67 LSQD+ID LL +I+S ++ D + P +K+ +YDF+RPDKFSKEQ+RT+S Sbjct: 5 LSQDEIDQLLTAISSGDASIED-------ARPISDTRKITLYDFRRPDKFSKEQMRTLSL 57 Query: 68 FHEAFARYTTTSLSALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDPLKGSAIF 127 HE FAR TTTSLSA LR MVHVHVASVDQLTYEEFIRSIP P+TLA+I MDPLKG+A+ Sbjct: 58 MHETFARLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVL 117 Query: 128 EVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIEQSVMESVIIRILANMREAWSQVVDLRP 187 EVDP+I F+I+DRLFGG G K + RDLT+IE SVME VI+RILAN+RE+W+QV+DLRP Sbjct: 118 EVDPSITFSIIDRLFGGTGQAAKVQ-RDLTDIENSVMEGVIVRILANVRESWTQVIDLRP 176 Query: 188 RFGHIEVNPQFAQIVPPTEMIILVTLEVKIGKVEGLMNFCLPYITIEPIVSKLSTRYWHS 247 R G IE NPQFAQIVPP+EM++LVTLE K+G+ EG+MNFC+PYITIEPI+SKLS+++W S Sbjct: 177 RLGQIETNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFS 236 Query: 248 LIGVGTTSENLDALREKLENTAMPLVAEIGEVKLKVREILSLDKGDVLNLESSLINKDLT 307 + +T++ + LR+KL M +VAE+G ++L VR+IL L GD++ L + + Sbjct: 237 SVRRSSTTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFV 296 Query: 308 LKVGTKEKFKCRMGLMGNKVSVQITEKIGDIKGFDLLKELTEEVE 352 L +G ++KF C+ G++G K++ QI E+I D +EL+ + E Sbjct: 297 LSIGNRKKFLCQPGVVGKKIAAQILERIESTSQED-FEELSADEE 340
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01400	OMPADOMAIN	55	8e-11	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 54.6 bits (131), Expect = 8e-11 Identities = 28/132 (21%), Positives = 57/132 (43%), Gaps = 21/132 (15%) Query: 124 ISLAADAFFDSASADVKLEENRDSIQKIASFIGFLSPRGYNFKIEGHTDNIDTDVNGPWK 183 +L +D F+ A +K E + ++ ++ S + L P+ + + G+TD I +D Sbjct: 215 FTLKSDVLFNFNKATLK-PEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSD-----A 268 Query: 184 SNWELSAARSVNMLEHILNYLDQSDVKRIENNFEVSGFGGSRPIATDDT---------PE 234 N LS R+ + +++YL + + G G S P+ + + Sbjct: 269 YNQGLSERRA----QSVVDYLISKGIPA--DKISARGMGESNPVTGNTCDNVKQRAALID 322 Query: 235 GRAYNRRIDILI 246 A +RR++I + Sbjct: 323 CLAPDRRVEIEV 334
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01415	FLGHOOKAP1	50	1e-08	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 50.3 bits (120), Expect = 1e-08 Identities = 18/89 (20%), Positives = 33/89 (37%), Gaps = 13/89 (14%) Query: 4 SLYSGVSGLQNHQTRMDVVGNNIANVNTIGFKKGRINFQDMISQSISGASRPTDARGGTN 63 + + +SGL Q ++ NNI++ N G+ + I + T GG Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTT---------IMAQANSTLGAGGW- 52 Query: 64 PKQVGLGMNVASIDTIHTQGAFQSTQKAS 92 VG G+ V+ + + + A Sbjct: 53 ---VGNGVYVSGVQREYDAFITNQLRAAQ 78 Score = 40.7 bits (95), Expect = 1e-05 Identities = 9/48 (18%), Positives = 28/48 (58%) Query: 394 IRSGVLEMANVDLAEQFTDMIVTQRGFQANAKTITTSDQLLQELVRLK 441 + + ++ V+L E++ ++ Q+ + ANA+ + T++ + L+ ++ Sbjct: 499 LSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01425	FLGHOOKFLIK	40	1e-05	Flagellar hook-length control protein signature.
>FLGHOOKFLIK#Flagellar hook-length control protein signature. Length = 375 Score = 39.8 bits (92), Expect = 1e-05 Identities = 24/82 (29%), Positives = 45/82 (54%), Gaps = 5/82 (6%) Query: 281 KLVLKPKELGSIRINLNLDSNNNLLGKIVVDNQNVKMLFDQNMHSLNKMLGESGFNASLN 340 +L L P++LG ++I+L +D N + ++V +Q+V+ + + L L ESG + Sbjct: 260 ELRLHPQDLGEVQISLKVDDNQAQI-QMVSPHQHVRAALEAALPVLRTQLAESGIQLGQS 318 Query: 341 LFLAGENLNSFTGNFKDDSKDQ 362 ++GE SF+G + S+ Q Sbjct: 319 -NISGE---SFSGQQQAASQQQ 336
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01430	TYPE4SSCAGX	29	0.016	Type IV secretion system CagX conjugation protein si...
>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein signature. Length = 522 Score = 29.0 bits (64), Expect = 0.016 Identities = 34/143 (23%), Positives = 66/143 (46%), Gaps = 20/143 (13%) Query: 39 RYFPEFVRTKLLGETSLVFDHNSNIILDEAR--LVKEREAIDIKNQQIEKLKEDLKLKED 96 R + EF++TK L+ D L+E + L KE+EA + + Q+ +K K + + +E Sbjct: 120 RDYQEFLKTK-----KLIVDAPDPKELEEQKKALEKEKEAKE-QAQKAQKDKREKRKEER 173 Query: 97 SLNKLEFELKQKQKDLDLKQKIIDDIINKYNDEEANILQTAVYLMNMPPEDAVKRLEDLN 156 + N+ E + + + N N L + D ++RLED+ Sbjct: 174 AKNRANLE------------NLTNAMSNPQNLSNNKNLSELIKQQRENELDQMERLEDMQ 221 Query: 157 PELAISYMRKIEELSKKEGRLSI 179 + + +++IEEL+KK+ ++ Sbjct: 222 EQAQANALKQIEELNKKQAEEAV 244
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01445	FLGFLIH	47	3e-08	Flagellar assembly protein FliH signature.
>FLGFLIH#Flagellar assembly protein FliH signature. Length = 228 Score = 46.7 bits (110), Expect = 3e-08 Identities = 43/197 (21%), Positives = 96/197 (48%), Gaps = 22/197 (11%) Query: 115 KESIETESNAEIER-LAREYEEKLKTDLDIAIAKGREEGYSKGY--------ESGFEDFD 165 +E+I E+ +E+ LA+ + + IA+GR++G+ +GY E G + Sbjct: 29 EETIIEEAEPSLEQQLAQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAK 88 Query: 166 KVMRKLHAIIASLIAERKGILESSSGQIVSLVMQIAIKVIKRITDSQKDI----VLENVN 221 +HA + L++E + L++ I S +MQ+A++ +++ + +++ + Sbjct: 89 SQQAPIHARMQQLVSEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQ 148 Query: 222 EVLKR---VKDKTQITIRVNLDDLDIVRHKKSDFISRFDIIENLEIIEDPNIGKGGCIIE 278 ++L++ K Q +RV+ DDL V +S + + DP + GGC + Sbjct: 149 QLLQQEPLFSGKPQ--LRVHPDDLQRVDDMLGATLS----LHGWRLRGDPTLHPGGCKVS 202 Query: 279 TNFGEIDARISSQLDKI 295 + G++DA ++++ ++ Sbjct: 203 ADEGDLDASVATRWQEL 219
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01450	FLGMOTORFLIG	427	e-153	Flagellar motor switch protein FliG signature.
>FLGMOTORFLIG#Flagellar motor switch protein FliG signature. Length = 344 Score = 427 bits (1100), Expect = e-153 Identities = 344/344 (100%), Positives = 344/344 (100%) Query: 1 MEEKKEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITS 60 MEEKKEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITS Sbjct: 1 MEEKKEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITS 60 Query: 61 ELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFV 120 ELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFV Sbjct: 61 ELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFV 120 Query: 121 RRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPE 180 RRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPE Sbjct: 121 RRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPE 180 Query: 181 VVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEI 240 VVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEI Sbjct: 181 VVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEI 240 Query: 241 KKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKED 300 KKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKED Sbjct: 241 KKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKED 300 Query: 301 MEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVLV 344 MEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVLV Sbjct: 301 MEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVLV 344
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01455	FLGMRINGFLIF	162	1e-45	Flagellar M-ring protein signature.
>FLGMRINGFLIF#Flagellar M-ring protein signature. Length = 559 Score = 162 bits (412), Expect = 1e-45 Identities = 112/565 (19%), Positives = 222/565 (39%), Gaps = 55/565 (9%) Query: 23 QKIALGLIIFFVILALVFLIGFSTKSQSIALF-GVEIKDQYLLDRISQRLDRENVKYFLS 81 +I L + + +V ++ ++ LF + +D I +L + N+ Y + Sbjct: 23 PRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQD---GGAIVAQLTQMNIPYRFA 79 Query: 82 SDGRIYLDDEKLAKKMRAILVREELVPVHMDPWALFDIDRWTITDFERSINLRRSITRAV 141 + ++R L ++ L + L D +++ I+ F +N +R++ + Sbjct: 80 NGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGEL 139 Query: 142 EQHIVALDDVDAVSVNLVMPEKALFKESQEPVKASVRITPRPGSDIITNRKKVEGLVKLI 201 + I L V + V+L MP+ +LF Q+ ASV +T PG + + ++ +V L+ Sbjct: 140 ARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRAL--DEGQISAVVHLV 197 Query: 202 QYAIEGLESDNIAIVDNSGTILNDFSNLDGIDRIDLAEKERKLKLKYEAMLRGEIDSALS 261 A+ GL N+ +VD SG +L SN G DL + + K E+ ++ I++ LS Sbjct: 198 SSAVAGLPPGNVTLVDQSGHLL-TQSNTSG---RDLNDAQLKFANDVESRIQRRIEAILS 253 Query: 262 KVLSVDRFMIARVNVKLDTSKETTESKEYAPIELQSQDPKASYNTRKVSDSTIISSQTQK 321 ++ A+V +LD + + + Y+P KA+ +R+++ S + + Sbjct: 254 PIVGNGNV-HAQVTAQLDFANKEQTEEHYSP---NGDASKATLRSRQLNISEQVGAGYPG 309 Query: 322 KEYQGQGYSPWGPPGQEGNTPPEYQDLSD-------------ITGKYNESQEIKNVALNE 368 P P TPP Q + + + E N ++ Sbjct: 310 GVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDR 369 Query: 369 KKSTSEKEPARIVGVSLGIFVDGIWNFVYDEKGDFVIENGMRKREYKPMALEEIKNIEDV 428 ++ I +S+ + V+ + + P+ +++K IED+ Sbjct: 370 TIRHTKMNVGDIERLSVAVVVNY---------------KTLADGKPLPLTADQMKQIEDL 414 Query: 429 LQSSFEYKPERGDSITVRNISFDRMNEFREIDENYFASER--FKYFLFIASIVFSLLILV 486 + + + +RGD++ V N F ++ E F ++ L + L++ Sbjct: 415 TREAMGFSDKRGDTLNVVNSPFSAVDN--TGGELPFWQQQSFIDQLLAAGRWLLVLVVAW 472 Query: 487 FTIFFAISRELERRRRLREEELAKQAHLRRQQALMDG-----GDDIGVDDVVGGIREGDE 541 A+ +L RR EE A Q + +Q + D + R G E Sbjct: 473 ILWRKAVRPQLTRR---VEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAE 529 Query: 542 LQSNA-ELLAREKPEDVAKLIRTWL 565 + S ++ P VA +IR W+ Sbjct: 530 VMSQRIREMSDNDPRVVALVIRQWM 554
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01460	FLGHOOKFLIE	86	2e-25	Flagellar hook-basal body complex protein FliE signa...
>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE signature. Length = 103 Score = 85.9 bits (212), Expect = 2e-25 Identities = 15/71 (21%), Positives = 38/71 (53%) Query: 40 TFKDVLINSITDVNKSQLNVSKVTEQAILKPSSIDVHDVVIAMSKANMNLSILKAVVERG 99 +F L ++ ++ +Q E+ L + ++DV+ M KA++++ + V + Sbjct: 32 SFAGQLHAALDRISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKL 91 Query: 100 VKAYQDIINIR 110 V AYQ++++++ Sbjct: 92 VAAYQEVMSMQ 102
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01465	FLGHOOKAP1	48	3e-09	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 47.6 bits (113), Expect = 3e-09 Identities = 21/80 (26%), Positives = 35/80 (43%), Gaps = 15/80 (18%) Query: 5 SSINVASTGLTAQRLRIDVISNNIANVSTSRTPDGGPYRRQRIIFAPRVNNPYWKGPFIP 64 S IN A +GL A + ++ SNNI++ + + Y RQ I A N Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVA------GYTRQTTIMAQ--ANSTLGA---- 49 Query: 65 DYLDNGIGQGVRVASIEKDK 84 +G GV V+ ++++ Sbjct: 50 ---GGWVGNGVYVSGVQREY 66 Score = 44.2 bits (104), Expect = 5e-08 Identities = 11/46 (23%), Positives = 23/46 (50%) Query: 104 KKGYVELPNVNLVEEMVDMISASRAYEANSTVINSSKSMFRSALAI 149 + VNL EE ++ + Y AN+ V+ ++ ++F + + I Sbjct: 500 SNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01475	HTHFIS	31	0.011	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 31.0 bits (70), Expect = 0.011 Identities = 13/70 (18%), Positives = 30/70 (42%), Gaps = 15/70 (21%) Query: 20 KYIIGQDEAKKLVSIALVNRYIRSRLPKEIKDEVMPKNIIMIGSTGIGKTEIAR---RLS 76 ++G+ A + + ++ R +++ L +++ G +G GK +AR Sbjct: 137 MPLVGRSAAMQEI-YRVLARLMQTDLT-----------LMITGESGTGKELVARALHDYG 184 Query: 77 KLIKAPFIKV 86 K PF+ + Sbjct: 185 KRRNGPFVAI 194
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01490	SYCDCHAPRONE	29	0.012	Gram-negative bacterial type III secretion SycD cha...
>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD chaperone signature. Length = 168 Score = 28.7 bits (64), Expect = 0.012 Identities = 16/91 (17%), Positives = 34/91 (37%), Gaps = 2/91 (2%) Query: 69 ARFFNLIGLEFFKLGQYGPAIEYFAKNLEINPNNYLSHFYIGVASYNLAKNLRVKDEVEK 128 +RFF +G +GQY AI ++ ++ F+ + + + Sbjct: 70 SRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFL 129 Query: 129 YI-ILAENSFLKSLSIR-DDFKDSLFAISNM 157 ++A+ + K LS R +++ M Sbjct: 130 AQELIADKTEFKELSTRVSSMLEAIKLKKEM 160
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01500	SHAPEPROTEIN	56	8e-11	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 55.9 bits (135), Expect = 8e-11 Identities = 50/226 (22%), Positives = 87/226 (38%), Gaps = 24/226 (10%) Query: 160 TGSSSSSQNLVR-CVNRAGFAVDEVVLGSLASSYATLSKEEREMGVLFIDMGKGTTDIIL 218 G++ + +R AG ++ +A++ G + +D+G GTT++ + Sbjct: 116 VGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAV 175 Query: 219 YIDGSPYYTGVIPIGVNRVTLDIAQVWK------VPEDVAENIKITAGIAHPSILESQME 272 Y+ + IG +R I + + E AE IK G A+P ++E Sbjct: 176 ISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIE 235 Query: 273 TVIIPNLGTRPPQ--EKSRKELSVIINSRLREIFEMMKAEI------LKRGLYNKINGGI 324 V NL P+ + E+ + L I + + L + + G+ Sbjct: 236 -VRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISER---GM 291 Query: 325 VLTGGGALFPGISNLIEEVFNYPARIGL-PMSINGIGE----EHID 365 VLTGGGAL + L+ E P + P++ G E ID Sbjct: 292 VLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMID 337

L144_RS01870

L144_RS01925

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
L144_RS01870	-1	11	0.803655	S-ribosylhomocysteine lyase
L144_RS01875	-1	15	1.312535	hypothetical protein
L144_RS01880	0	18	1.687228	HIT family protein
L144_RS01885	0	17	2.163502	magnesium transporter
L144_RS01890	0	17	2.367083	hypothetical protein
L144_RS01895	-1	18	3.645882	BMP family protein
L144_RS01900	0	20	4.142684	BMP family protein
L144_RS01905	1	19	4.498236	BMP family protein
L144_RS01910	1	19	5.057160	BMP family protein
L144_RS01915	2	19	4.982574	30S ribosomal protein S7
L144_RS01920	1	19	4.793305	30S ribosomal protein S12
L144_RS01925	0	19	4.770614	DNA-directed RNA polymerase subunit beta'

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01870	LUXSPROTEIN	188	3e-64	Bacterial autoinducer-2 (AI-2) production protein Lu...
>LUXSPROTEIN#Bacterial autoinducer-2 (AI-2) production protein LuxS signature. Length = 171 Score = 188 bits (479), Expect = 3e-64 Identities = 52/162 (32%), Positives = 86/162 (53%), Gaps = 11/162 (6%) Query: 4 ITSFTIDHTKLN-PGIYVSR-KDTFENVIFTTIDIRIKAPNIEPIIENAAIHTIEHIGAT 61 + SFT+DHT++N P + V++ T + T D+R APN + I+ IHT+EH+ A Sbjct: 3 LDSFTVDHTRMNAPAVRVAKTMQTPKGDTITVFDLRFTAPN-KDILSEKGIHTLEHLYAG 61 Query: 62 LLRNN-EVWTEKIVYFGPMGCRTGFYLIIFGDYESKDLVDLVSWLFSE----IVNFSEPI 116 +RN+ + +I+ PMGCRTGFY+ + G + + D +W+ + V I Sbjct: 62 FMRNHLNGDSVEIIDISPMGCRTGFYMSLIGTPSEQQVAD--AWIAAMEDVLKVENQNKI 119 Query: 117 PGASDKECGNYKEHNLDMAKYESSKYLQI-LNNIKEENLKYP 157 P ++ +CG H+LD AK + L++ + K + L P Sbjct: 120 PELNEYQCGTAAMHSLDEAKQIAKNILEVGVAVNKNDELALP 161
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01895	LIPPROTEIN48	74	8e-17	Mycoplasma P48 major surface lipoprotein signature.
>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature. Length = 428 Score = 74.3 bits (182), Expect = 8e-17 Identities = 83/340 (24%), Positives = 127/340 (37%), Gaps = 34/340 (10%) Query: 7 IFGILLTSCFSRNGIESSS-KKIKISMLVD-GVLDDKSFNSSANEALLRLKKDFPENIEE 64 I T+ + ++++ K+K ++ D G +DDKSFN SA EAL + K IE Sbjct: 40 ISKYTTTNANGKQVVKNAELLKLKPVLITDEGKIDDKSFNQSAFEALKAINKQ--TGIEI 97 Query: 65 VFSCAISGVYSSYVSDLDNLKRNGSDLIWLVGYMLTDASL--LVSSENPKISYGIIDPIY 122 S S+Y S L + IW++ S+ + + ++ I I Sbjct: 98 NNVEPSSNFESAYNSALSAGHK-----IWVLNGFKHQQSIKQYIDAHREELERNQIK-II 151 Query: 123 GDDVQIPEN---LIAVVFRVEQGAFLAGYIAAKKSFSGK------IGFIGGMKGNIVDAF 173 G D I ++ F +++ AF GY A S + + GG V F Sbjct: 152 GIDFDIETEYKWFYSLQFNIKESAFTTGY-AIASWLSEQDESKRVVASFGGGAFPGVTTF 210 Query: 174 RYGYESGAKYANKDIEIISEYSNSFSDVDIGRT-----------IASKMYSKGIDVIHFA 222 G+ G Y N+ + Y S +D G T + S + H Sbjct: 211 NEGFAKGILYYNQKHKSSKIYHTSPVKLDSGFTAGEKMNTVINNVLSSTPADVKYNPHVI 270 Query: 223 AGLAGIGVIETAKNLGDGYYVIGADQDQSY-LAPKNFITSVIKNIGDALYLITGEYIKNN 281 +AG ET + G YVIG D DQ +TSV+K+I A+Y + I Sbjct: 271 LSVAGPATFETVRLANKGQYVIGVDSDQGMIQDKDRILTSVLKHIKQAVYETLLDLILEK 330 Query: 282 NVWEGGKVVQMGLRDGVIGLPNANEFEYIKVLERKIINKE 321 VV+ D + ++I V E N E Sbjct: 331 EEGYKPYVVKDKKADKKWSHFGTQKEKWIGVAENHFSNTE 370
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01900	LIPPROTEIN48	70	2e-15	Mycoplasma P48 major surface lipoprotein signature.
>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature. Length = 428 Score = 69.7 bits (170), Expect = 2e-15 Identities = 66/261 (25%), Positives = 99/261 (37%), Gaps = 29/261 (11%) Query: 30 KVSLIID-GTFDDKSFNESALNGVKKVKEEFKIELVLKESSSNSYLSDLEGLKDAGSDLI 88 K LI D G DDKSFN+SA +K + ++ IE+ E SSN + S AG + Sbjct: 63 KPVLITDEGKIDDKSFNQSAFEALKAINKQTGIEINNVEPSSN-FESAYNSALSAGHKIW 121 Query: 89 WLIGYRFS------DVAKVAALQNPDMKYAIID-PIYSNDPIPANLVGMTFRAQEGAFLT 141 L G++ A L+ +K ID I + + F +E AF T Sbjct: 122 VLNGFKHQQSIKQYIDAHREELERNQIKIIGIDFDIETEYKW---FYSLQFNIKESAFTT 178 Query: 142 GYIAAKLSKTGK-----IGFLGGIEGEIVDAFRYGYEAGAKYANKD-----------IKI 185 GY A + GG V F G+ G Y N+ +K+ Sbjct: 179 GYAIASWLSEQDESKRVVASFGGGAFPGVTTFNEGFAKGILYYNQKHKSSKIYHTSPVKL 238 Query: 186 STQYIGSFADLEAGRSVATRMYSDEIDIIHHAAGLGGIGAIEVAKELGSGHYIIGVDEDQ 245 + + +V + +D H + G E + G Y+IGVD DQ Sbjct: 239 DSGFTAGEKMNTVINNVLSSTPADVKYNPHVILSVAGPATFETVRLANKGQYVIGVDSDQ 298 Query: 246 AY-LAPDNVITSTTKDVGRAL 265 D ++TS K + +A+ Sbjct: 299 GMIQDKDRILTSVLKHIKQAV 319
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01905	LIPPROTEIN48	49	2e-08	Mycoplasma P48 major surface lipoprotein signature.
>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature. Length = 428 Score = 48.8 bits (116), Expect = 2e-08 Identities = 58/296 (19%), Positives = 103/296 (34%), Gaps = 50/296 (16%) Query: 18 FKSNKKSIKSDKV----VVGVLAHGSFYDKGYNQSVHDGVVKLRDNFGIKLITKSLRPYP 73 + K+ +K+ ++ V + G DK +NQS + + + GI++ Sbjct: 47 NANGKQVVKNAELLKLKPVLITDEGKIDDKSFNQSAFEALKAINKQTGIEINNVE----- 101 Query: 74 IEGKRLLTVDEAMTEDAYEVQKNPLNLFW-LIGYRFSDLSVKL------SYERPDIYYGI 126 + E AY + + W L G++ + ER I Sbjct: 102 ---------PSSNFESAYNSALSAGHKIWVLNGFKHQQSIKQYIDAHREELERNQI---K 149 Query: 127 IDAFDYGDIQVPKNSLAIKFRNEEAAFLAGYIAA-----KMSRKEKIGFLTGPMSEHLKD 181 I D+ K +++F +E+AF GY A + K + G + Sbjct: 150 IIGIDFDIETEYKWFYSLQFNIKESAFTTGYAIASWLSEQDESKRVVASFGGGAFPGVTT 209 Query: 182 FKFGFKAGIFYAN---PKLRLVSK---KAPSLFDKEKGKAMAL-------FMYKEDKVGV 228 F GF GI Y N ++ K S F + + + V Sbjct: 210 FNEGFAKGILYYNQKHKSSKIYHTSPVKLDSGFTAGEKMNTVINNVLSSTPADVKYNPHV 269 Query: 229 IFPIAGITGLGVYDAAKELGPKYYVIGLNQDQSYI-APQNVITSIIKDIGKVIYSI 283 I +AG ++ + YVIG++ DQ I ++TS++K I + +Y Sbjct: 270 ILSVAGPA---TFETVRLANKGQYVIGVDSDQGMIQDKDRILTSVLKHIKQAVYET 322
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01910	LIPPROTEIN48	60	3e-12	Mycoplasma P48 major surface lipoprotein signature.
>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature. Length = 428 Score = 60.0 bits (145), Expect = 3e-12 Identities = 55/272 (20%), Positives = 97/272 (35%), Gaps = 31/272 (11%) Query: 28 KTVSLIVDGAFDDKGFNESSSKAIRKLKADLNINIIEKASTGNSYLGDIANLEDGNSNLI 87 K V + +G DDK FN+S+ +A++ + I I +++ + + Sbjct: 63 KPVLITDEGKIDDKSFNQSAFEALKAINKQTGIE-INNVEPSSNFESAYNSALSAGHKIW 121 Query: 88 WGIGFRLSDILFQ---RASENVSVNYAIIEGV-YDEIQIPKNLLNISFRSEEVAFLAGY- 142 GF+ + Q E + N I G+ +D K ++ F +E AF GY Sbjct: 122 VLNGFKHQQSIKQYIDAHREELERNQIKIIGIDFDIETEYKWFYSLQFNIKESAFTTGYA 181 Query: 143 ----FASKASKTGKIGFVGGVRGKVLESFMYGYEAGAKYANSNIKVVSQYVGTFGDFGLG 198 + + + GG + +F G+ G Y N K Y + G Sbjct: 182 IASWLSEQDESKRVVASFGGGAFPGVTTFNEGFAKGILYYNQKHKSSKIYHTSPVKLDSG 241 Query: 199 ---------------RSTASNMYRDGVDIIFAAAGLSGIGVIEAAKELGPDHYIIGVDQD 243 ST +++ + I+ A + E + Y+IGVD D Sbjct: 242 FTAGEKMNTVINNVLSSTPADVKYNPHVILSVAGPATF----ETVRLANKGQYVIGVDSD 297 Query: 244 QSY-LAPNNVIVSAVKKVDSLMYS-LTKKYLE 273 Q + ++ S +K + +Y L LE Sbjct: 298 QGMIQDKDRILTSVLKHIKQAVYETLLDLILE 329
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS01925	RTXTOXIND	31	0.037	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 31.0 bits (70), Expect = 0.037 Identities = 7/17 (41%), Positives = 14/17 (82%) Query: 1192 KHLLVRDGDVVKAGDML 1208 K ++V++G+ V+ GD+L Sbjct: 108 KEIIVKEGESVRKGDVL 124

L144_RS03915

L144_RS03935

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
L144_RS03915	1	22	0.737489	muramidase
L144_RS03920	1	17	1.356275	flagellar P-ring protein
L144_RS03925	0	12	2.595460	hypothetical protein
L144_RS03930	0	11	3.450788	flagellar basal-body rod protein FlgG
L144_RS03935	3	16	2.786620	flagellar basal-body rod protein FlgF

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS03915	FLGFLGJ	48	7e-10	Flagellar protein FlgJ signature.
>FLGFLGJ#Flagellar protein FlgJ signature. Length = 313 Score = 47.8 bits (113), Expect = 7e-10 Identities = 21/69 (30%), Positives = 39/69 (56%), Gaps = 2/69 (2%) Query: 34 DLRKASLEFEAMFIKQMLESMKKTLNKDQNLLNGGQVEEIFEDMLCEQRAKQMAQAQSFG 93 ++R + + E MF++ ML+SM+ L KD L + ++ M +Q A+QM + G Sbjct: 32 NIRPVARQVEGMFVQMMLKSMRDALPKDG--LFSSEHTRLYTSMYDQQIAQQMTAGKGLG 89 Query: 94 LADLIYNQL 102 LA+++ Q+ Sbjct: 90 LAEMMVKQM 98
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS03920	FLGPRINGFLGI	257	9e-86	Flagellar P-ring protein signature.
>FLGPRINGFLGI#Flagellar P-ring protein signature. Length = 373 Score = 257 bits (658), Expect = 9e-86 Identities = 83/352 (23%), Positives = 153/352 (43%), Gaps = 60/352 (17%) Query: 35 SLSESVKLKEIADIYPTNTNFLTGIGIVAGLAGKGDSIKQKDL----IIKILEENNIINE 90 + +++ ++K+IA + N L G G+V GL G GDS++ + +L+ I + Sbjct: 24 AQADTSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSMRAMLQNLGITTQ 83 Query: 91 IGSNNIESKNIALVNVSLQVKGNTIKGSKHKACVASILDSKDLTNGILLKTNLKNKEGEI 150 G +N +KNIA V V+ + GS+ V+S+ D+ L G L+ T+L +G+I Sbjct: 84 GGQSN--AKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLIMTSLSGADGQI 141 Query: 151 IAIASGITQPNN-KLKGSGYTI-----------DSVIINEN--QNINHSYNIILKKGN-- 194 A+A G N +G T+ + II S N++L+ N Sbjct: 142 YAVAQGALIVNGFSAQGDAATLTQGVTTSARVPNGAIIERELPSKFKDSVNLVLQLRNPD 201 Query: 195 YTLINRIHKILTS---KKINNKI---KSDSTIEIEAKNIS----LLEEIENIKIETN--P 242 ++ R+ ++ + + + I + I ++ ++ L+ EIEN+ +ET+ Sbjct: 202 FSTAVRVADVVNAFARARYGDPIAEPRDSQEIAVQKPRVADLTRLMAEIENLTVETDTPA 261 Query: 243 KILIDKKNGIILASENAKI-------GTFTFSIEKDNQNI----FLSKNNKTTIQVNSMK 291 K++I+++ G I+ + +I GT T + + Q I F Q + M Sbjct: 262 KVVINERTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQPAPFSRGQTAVQPQTDIMA 321 Query: 292 LNE----FILK-----------NSNNLSNKELIQIIQAAQKINKLNGELILE 328 + E I++ NS L +I I+Q + L EL+L+ Sbjct: 322 MQEGSKVAIVEGPDLRTLVAGLNSIGLKADGIIAILQGIKSAGALQAELVLQ 373
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS03930	FLGHOOKAP1	45	2e-07	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 44.9 bits (106), Expect = 2e-07 Identities = 10/44 (22%), Positives = 23/44 (52%) Query: 220 ILEMSNVSIAEEMVTMIVAQRAYEINSKAIQTSDNMLGIANNLK 263 +S V++ EE + Q+ Y N++ +QT++ + N++ Sbjct: 503 QQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546 Score = 39.6 bits (92), Expect = 1e-05 Identities = 19/79 (24%), Positives = 34/79 (43%), Gaps = 14/79 (17%) Query: 5 LWTAASGMTAQQYNVDTIANNLSNVNTTGFKKIRAEFEDLIYQTHNRAGTPATENTLRPL 64 + A SG+ A Q ++T +NN+S+ N G+ + A N+ Sbjct: 4 INNAMSGLNAAQAALNTASNNISSYNVAGYTRQTT--------------IMAQANSTLGA 49 Query: 65 GNQVGHGTKIAATQRIFEQ 83 G VG+G ++ QR ++ Sbjct: 50 GGWVGNGVYVSGVQREYDA 68
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
L144_RS03935	FLGHOOKAP1	43	8e-07	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 43.0 bits (101), Expect = 8e-07 Identities = 14/64 (21%), Positives = 28/64 (43%) Query: 214 DTKTSGKAQEIDISLRPKIETETLEASNVNAVKEMVLMIEINRAYEANQKTIQTEDSLLG 273 T T + ++ ++ + S VN +E + + Y AN + +QT +++ Sbjct: 481 KTATLKTSSATQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFD 540 Query: 274 KLIN 277 LIN Sbjct: 541 ALIN 544 Score = 38.4 bits (89), Expect = 2e-05 Identities = 13/39 (33%), Positives = 23/39 (58%) Query: 4 GIYTAASGMMAERRKLDTVSNNLANIDLIGYKKDLSIQK 42 I A SG+ A + L+T SNN+++ ++ GY + +I Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMA 41