PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
Genomesacchari.gbThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in NC_021219 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1L336_RS05400L336_RS00195Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS05400-1163.386436prepilin-type N-terminal cleavage/methylation
L336_RS001300163.369795histidine--tRNA ligase
L336_RS00135-1173.777439TrmH family RNA methyltransferase
L336_RS054050163.760204hypothetical protein
L336_RS001500143.726673*hypothetical protein
L336_RS054100194.999391prepilin-type N-terminal cleavage/methylation
L336_RS001601257.084730hypothetical protein
L336_RS001652358.569657FAD-binding oxidoreductase
L336_RS0017034110.225074hypothetical protein
L336_RS001804409.517128*hypothetical protein
L336_RS058101337.621639hypothetical protein
L336_RS001900316.907501Mov34/MPN/PAD-1 family protein
L336_RS00195-1194.095936ThiF family adenylyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS05400BCTERIALGSPG461e-08 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

		          signature.

		          Length = 145

		

		 Score = 45.6 bits (108), Expect = 1e-08

		 Identities = 24/78 (30%), Positives = 43/78 (55%), Gaps = 3/78 (3%)

		

		Query: 1  MRLRHVRSGFTIVELLVVIVVIAILATLLTVIYMDSQLQARDTQVRNGAHKVAEALGVWA 60

		          MR    + GFT++E++VVIV+I +LA+L+    M ++ +A   +  +    +  AL ++ 

		Sbjct: 1  MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYK 60

		

		Query: 61 ANNGGRFPAG--GLSSTV 76

		           +N   +P    GL S V

		Sbjct: 61 LDN-HHYPTTNQGLESLV 77
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS05410BCTERIALGSPG444e-08 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

		          signature.

		          Length = 145

		

		 Score = 43.7 bits (103), Expect = 4e-08

		 Identities = 19/60 (31%), Positives = 37/60 (61%)

		

		Query: 6  KGFTILELIVVIVVIGTLTTITVVAFNGIQDRAYMSQIYANVSSTAKLMNSYHIFNRTYP 65

		          +GFT+LE++VVIV+IG L ++ V    G +++A   +  +++ +    ++ Y + N  YP

		Sbjct: 8  RGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLDNHHYP 67
2L336_RS00260L336_RS00390Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS002602223.958027transcription termination factor NusA
L336_RS002652296.010548hypothetical protein
L336_RS058503336.397915hypothetical protein
L336_RS058553315.207254hypothetical protein
L336_RS002801274.806815tRNA preQ1(34) S-adenosylmethionine
L336_RS002852253.755850hypothetical protein
L336_RS002901223.752967hypothetical protein
L336_RS002950193.227984tRNA guanosine(34) transglycosylase Tgt
L336_RS003001193.553584queuosine precursor transporter
L336_RS003050173.313056HNH endonuclease family protein
L336_RS003100172.716945M1 family metallopeptidase
L336_RS054200193.540427ribonuclease HII
L336_RS003200162.52850050S ribosomal protein L19
L336_RS003253172.633116hypothetical protein
L336_RS003302171.545222hypothetical protein
L336_RS003351131.037868hypothetical protein
L336_RS003401121.116077NADP-dependent malic enzyme
L336_RS003450110.440899FHA domain-containing protein
L336_RS003501110.020461GspE/PulE family protein
L336_RS00355110-1.168055hypothetical protein
L336_RS00360111-0.692790DNA polymerase III subunit alpha
L336_RS00365316-0.830776DUF5665 domain-containing protein
L336_RS054251140.703067YtxH domain-containing protein
L336_RS003751141.242030hypothetical protein
L336_RS003800172.591190NTP transferase domain-containing protein
L336_RS003850173.034516Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
L336_RS003900183.334136hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS00390SECYTRNLCASE290.007 Preprotein translocase SecY subunit signature.
		>SECYTRNLCASE#Preprotein translocase SecY subunit signature.

		          Length = 437

		

		 Score = 29.0 bits (65), Expect = 0.007

		 Identities = 7/33 (21%), Positives = 19/33 (57%)

		

		Query: 1   MTQNPASFFLLVSVLIVAIILVVYLTMSRRGVP 33

		           +      F  +++V ++ + LVV++  ++R +P

		Sbjct: 215 LAGGWIEFGTVIAVGLIMVALVVFVEQAQRRIP 247
3L336_RS00565L336_RS00655Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS005652150.037652alpha/beta hydrolase
L336_RS00570215-0.873871cytidine deaminase
L336_RS00575114-0.733539alpha/beta fold hydrolase
L336_RS00580114-1.332434phenylalanine--tRNA ligase subunit alpha
L336_RS00585116-0.740344hypothetical protein
L336_RS00590-113-0.445358aquaporin
L336_RS00595-215-0.998689DNA recombination protein RmuC
L336_RS00600-217-2.731494hypothetical protein
L336_RS00605-117-3.184493phosphatase PAP2 family protein
L336_RS00610-118-3.713255hypothetical protein
L336_RS00615120-6.073447M20/M25/M40 family metallo-hydrolase
L336_RS05445324-7.033569helix-turn-helix domain-containing protein
L336_RS05450326-7.402967*recombinase family protein
L336_RS05865225-7.227995helix-turn-helix domain-containing protein
L336_RS00635224-6.773450hypothetical protein
L336_RS00640223-6.875549site-specific DNA-methyltransferase
L336_RS00645-118-3.994215DEAD/DEAH box helicase family protein
L336_RS00650221-1.322825hypothetical protein
L336_RS00655220-0.762934sulfite exporter TauE/SafE family protein
4L336_RS05815L336_RS00880Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS058152274.729211hypothetical protein
L336_RS007101172.113259ribonuclease III
L336_RS007151151.416980NUDIX domain-containing protein
L336_RS007200141.519374transcription antitermination factor NusB
L336_RS05870-1131.253654hypothetical protein
L336_RS00725-1131.008781hypothetical protein
L336_RS05470-29-0.270164leucine--tRNA ligase
L336_RS00740011-1.410741hypothetical protein
L336_RS00745316-2.168477hypothetical protein
L336_RS05475823-4.050284*hypothetical protein
L336_RS00760727-4.095234hypothetical protein
L336_RS00765729-4.041335septation protein SpoVG family protein
L336_RS00770630-3.705976hypothetical protein
L336_RS00775628-3.664304hypothetical protein
L336_RS00780629-3.492037helix-turn-helix domain-containing protein
L336_RS00785528-2.890792cation diffusion facilitator family transporter
L336_RS00790426-3.379975cation diffusion facilitator family transporter
L336_RS00795527-3.906404hypothetical protein
L336_RS00800629-5.363007hypothetical protein
L336_RS00805630-5.661868metal-sensitive transcriptional regulator
L336_RS00810731-5.850597hypothetical protein
L336_RS00815529-7.083837DUF305 domain-containing protein
L336_RS00820630-7.489154heavy metal translocating P-type ATPase
L336_RS00825630-8.734708recombinase family protein
L336_RS05875325-7.540010hypothetical protein
L336_RS00830325-7.442013hypothetical protein
L336_RS00835224-7.427433hypothetical protein
L336_RS00840122-5.698931hypothetical protein
L336_RS00845220-5.313844membrane protein of unknown function
L336_RS00855219-3.840147right-handed parallel beta-helix
L336_RS00860320-5.840390DMT family transporter
L336_RS05880318-5.026884hypothetical protein
L336_RS00870217-4.169751reverse transcriptase family protein
L336_RS00875218-4.530787hypothetical protein
L336_RS00880118-3.884067hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS00825CHANNELTSX300.015 Nucleoside-specific channel-forming protein Tsx signa...
		>CHANNELTSX#Nucleoside-specific channel-forming protein Tsx

		           signature.

		          Length = 294

		

		 Score = 30.4 bits (68), Expect = 0.015

		 Identities = 24/94 (25%), Positives = 36/94 (38%), Gaps = 12/94 (12%)

		

		Query: 43  WWS------GSVSSKRGSNVQRKDLLEIYDFAKKNKRVKYLIVDEPDRFMRSIDESGFWE 96

		           WW       GS  ++ G  ++    LE   FAKK+    Y  +D P  F  +    G W 

		Sbjct: 34  WWHQSVNVVGSYHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYIDAPVFFGGNSTAKGIW- 92

		

		Query: 97  VKLFYETGTRVWYASNPELNKDDLPSKLLKFTKF 130

		                  G+ ++    P  + D L +  L F  F

		Sbjct: 93  -----NKGSPLFMEIEPRFSIDKLTNTDLSFGPF 121
5L336_RS01160L336_RS01245Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS011602170.902624hypothetical protein
L336_RS011651181.716842magnesium transporter CorA family protein
L336_RS011702201.146522DUF2892 domain-containing protein
L336_RS011752180.657049DNA alkylation repair protein
L336_RS011802190.792331DUF1697 domain-containing protein
L336_RS055003170.707395CPBP family intramembrane metalloprotease
L336_RS011903180.343515hypothetical protein
L336_RS01195317-0.141122DUF3267 domain-containing protein
L336_RS055053190.248547DUF5668 domain-containing protein
L336_RS01205220-0.719397peptide-methionine (S)-S-oxide reductase MsrA
L336_RS01210320-1.005871peptide-methionine (R)-S-oxide reductase MsrB
L336_RS01215220-1.337571zinc ribbon domain-containing protein
L336_RS012203150.383321MFS transporter
L336_RS012252200.022215PadR family transcriptional regulator
L336_RS01230218-0.221378hypothetical protein
L336_RS012352180.490262DUF1801 domain-containing protein
L336_RS012402160.631897DUF1801 domain-containing protein
L336_RS012452150.105466hypothetical protein
6L336_RS01425L336_RS01540Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS01425-3183.068504triose-phosphate isomerase
L336_RS01430-2286.858564phosphoglycerate kinase
L336_RS01435-2265.548935pyruvate kinase
L336_RS01440-2225.008517cellulase family glycosylhydrolase
L336_RS01445-2235.333850VIT family protein
L336_RS01455-2235.311023*hypothetical protein
L336_RS01460-1184.011106hypothetical protein
L336_RS01465-1120.284726DUF11 domain-containing protein
L336_RS014700130.160348HAMP domain-containing histidine kinase
L336_RS01475112-1.178328hypothetical protein
L336_RS01480113-1.924984response regulator
L336_RS01485014-3.571927bifunctional 5,10-methylenetetrahydrofolate
L336_RS01490116-4.444210hypothetical protein
L336_RS01500017-4.735418*hypothetical protein
L336_RS01505017-4.947576hypothetical protein
L336_RS01510016-3.822844hypothetical protein
L336_RS01515016-3.763299hypothetical protein
L336_RS05530-117-1.712028*UDP-N-acetylmuramate dehydrogenase
L336_RS01525018-1.497382type II secretion system GspH family protein
L336_RS01535420-2.915119hypothetical protein
L336_RS01540217-2.230085type II secretion system GspH family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS01445RTXTOXIND290.022 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

		           signature.

		          Length = 478

		

		 Score = 28.6 bits (64), Expect = 0.022

		 Identities = 16/97 (16%), Positives = 36/97 (37%), Gaps = 5/97 (5%)

		

		Query: 74  EYVSVSSQSDAEKAYIELEKADLKDNPEDELDELAREYQKLGLSKQTSHRVAAELTEKNA 133

		           +YV   ++    K+ +E  ++++  + ++E   + + ++   L K         L     

		Sbjct: 260 KYVEAVNELRVYKSQLEQIESEI-LSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLEL 318

		

		Query: 134 LKAHLHVHFNLDPEDINSPMHAAIASLLAFTAGGLVP 170

		            K             I +P+   +  L   T GG+V 

		Sbjct: 319 AKNE----ERQQASVIRAPVSVKVQQLKVHTEGGVVT 351
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS01460PF03544361e-04 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

		          Length = 243

		

		 Score = 36.5 bits (84), Expect = 1e-04

		 Identities = 17/47 (36%), Positives = 19/47 (40%), Gaps = 2/47 (4%)

		

		Query: 404 ANPGTDESVAPTDAGQPPAVYTPPAVPAPPVVDTPPAPTPEQQAPKP 450

		           A P +   VAP D   P AV  PP     P  +  P P PE     P

		Sbjct: 47  AQPISVTMVAPADLEPPQAVQPPPEPVVEP--EPEPEPIPEPPKEAP 91
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS01470PF06580310.018 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

		          Length = 349

		

		 Score = 31.0 bits (70), Expect = 0.018

		 Identities = 17/103 (16%), Positives = 36/103 (34%), Gaps = 25/103 (24%)

		

		Query: 585 VIMNFIDNAIYY----SPEGSTIAIRLKVEAGAAVLTVKDSGMGVPKSEQKHLFTKFFRA 640

		           ++   ++N I +     P+G  I ++   + G   L V+++G    K+            

		Sbjct: 259 LVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNT----------- 307

		

		Query: 641 ENARKQRPDGTGIGLFLAKKVVDAHGG---ALVFESLPGKGST 680

		                   + TG GL   ++ +    G    +      GK + 

		Sbjct: 308 -------KESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNA 343
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS01480HTHFIS741e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

		          Length = 484

		

		 Score = 73.7 bits (181), Expect = 1e-18

		 Identities = 21/103 (20%), Positives = 40/103 (38%), Gaps = 2/103 (1%)

		

		Query: 2   TKIAIIEDDQVINQMYRMKFEAAGFEVSTAGDGETGVALVKKVTPDIILLDLQMPHMNGA 61

		             I + +DD  I  +       AG++V    +  T    +     D+++ D+ MP  N  

		Sbjct: 4   ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

		

		Query: 62  EALTAIRGNAASSKTPVIILTNLGEEEAPKNLRSLGIHSYIVK 104

		           + L  I+   A    PV++++              G + Y+ K

		Sbjct: 64  DLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPK 104
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS01530BCTERIALGSPG466e-09 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

		          signature.

		          Length = 145

		

		 Score = 46.0 bits (109), Expect = 6e-09

		 Identities = 16/72 (22%), Positives = 35/72 (48%), Gaps = 8/72 (11%)

		

		Query: 1  MAERRGFTIVELVIVMVIMAILLTLTISGVTSGQVSARDSERKADAENIARGLERYYNEV 60

		            ++RGFT++E+++V+VI+ +L +L +  +   +  A   +  +D   +   L+ Y  + 

		Sbjct: 4  TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLD- 62

		

		Query: 61 AKPSVGRTGRYP 72

		                    YP

		Sbjct: 63 -------NHHYP 67
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS01540BCTERIALGSPG334e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

		          signature.

		          Length = 145

		

		 Score = 32.9 bits (75), Expect = 4e-04

		 Identities = 22/88 (25%), Positives = 37/88 (42%), Gaps = 12/88 (13%)

		

		Query: 5  KTASQPGFTLVEMLVVAPIVILVVGGIVALLIALVGDVLIARERNSMAYNTQDALNLIEQ 64

		           T  Q GFTL+E++VV  IVI+       +L +LV   L+  +  +        +  +E 

		Sbjct: 3  ATDKQRGFTLLEIMVV--IVII------GVLASLVVPNLMGNKEKADKQKAVSDIVALEN 54

		

		Query: 65 DVRLSSNIQATTGTLPSP-QGSDSNVSG 91

		           +      +      P+  QG +S V  

		Sbjct: 55 AL---DMYKLDNHHYPTTNQGLESLVEA 79
7L336_RS02170L336_RS02285Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS021704192.709461F0F1 ATP synthase subunit beta
L336_RS021755192.029189ATP synthase epsilon chain
L336_RS021805191.651412hypothetical protein
L336_RS021857222.208511hypothetical protein
L336_RS021907223.651057hypothetical protein
L336_RS021957213.220093hypothetical protein
L336_RS022006213.381770hypothetical protein
L336_RS022056203.685511PKD domain-containing protein
L336_RS022106193.682880exported protein of unknown function
L336_RS059054132.875656hypothetical protein
L336_RS02220-115-0.860378hypothetical protein
L336_RS02225016-0.393951hypothetical protein
L336_RS02230114-0.529966hypothetical protein
L336_RS02235213-0.434789trypsin-like peptidase domain-containing
L336_RS02240313-0.617235DUF5305 family protein
L336_RS05600414-0.116395signal peptidase I
L336_RS02250416-1.071086hypothetical protein
L336_RS02255317-2.170706hypothetical protein
L336_RS02260119-3.333162hypothetical protein
L336_RS02265119-3.856155tryptophan-rich sensory protein
L336_RS02270119-4.625857glutathione peroxidase
L336_RS05605219-4.383376LemA family protein
L336_RS02280119-3.620849hypothetical protein
L336_RS02285214-2.013818hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS02205MICOLLPTASE310.008 Microbial collagenase metalloprotease (M9) signature.
		>MICOLLPTASE#Microbial collagenase metalloprotease (M9) signature.

		          Length = 1104

		

		 Score = 30.8 bits (69), Expect = 0.008

		 Identities = 17/63 (26%), Positives = 25/63 (39%), Gaps = 9/63 (14%)

		

		Query: 206 VNIQW--GDGTNKVVSRTDNQTFRVGHVYEKAGTYQLSLQATDADGRVAFLTVASIVNGQ 263

		              +W  GDG         N+  +  H Y K G Y++ L  TD +G +   +    V   

		Sbjct: 806 KAYEWDFGDGE------KSNEA-KATHKYNKTGEYEVKLTVTDNNGGINTESKKIKVVED 858

		

		Query: 264 PPV 266

		            PV

		Sbjct: 859 KPV 861
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS05905cloacin507e-08 Cloacin signature.
		>cloacin#Cloacin signature.

		          Length = 551

		

		 Score = 50.1 bits (119), Expect = 7e-08

		 Identities = 36/104 (34%), Positives = 45/104 (43%), Gaps = 5/104 (4%)

		

		Query: 530 AGGAGGTGGTNAGSAGSSLTGGLGGDGRTSGTTDGSGASGGLASGGNGGGVIATSRAGGG 589

		           +GG G    T A S   ++ GG  G G   G +DGSG S      G G G   +    GG

		Sbjct: 2   SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSG---SGIHWGG 58

		

		Query: 590 GGGAGYFGGGGGSSSSSGSGAGGGGSGSSFVSG--SLTTTAGGG 631

		           G G G  GG G S   SG+G       +    G  +L+T   GG

		Sbjct: 59  GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102

		

		

		

		 Score = 47.4 bits (112), Expect = 5e-07

		 Identities = 43/137 (31%), Positives = 52/137 (37%), Gaps = 28/137 (20%)

		

		Query: 502 AGGAGGGTNGIAGTTSGAGGGGGGGTQSAGGAGGTGGTNAGSAGSSLTGGLGGDGRTSGT 561

		           +GG G G N  A +TSG   GG        G G  GG + GS  SS     GG       

		Sbjct: 2   SGGDGRGHNTGAHSTSGNINGG------PTGLGVGGGASDGSGWSSENNPWGG------- 48

		

		Query: 562 TDGSGASGGLASGGNGGGVIATSRAGGGGGGAGYFGGGGGSSSSSG--------SGAGGG 613

		             GSG   G  SG   GG       G G  G G   GG  S+ ++         S  G G

		Sbjct: 49  GSGSGIHWGGGSGHGNGG-------GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAG 101

		

		Query: 614 GSGSSFVSGSLTTTAGG 630

		           G   S  +G+L+     

		Sbjct: 102 GLAVSISAGALSAAIAD 118

		

		

		

		 Score = 45.9 bits (108), Expect = 1e-06

		 Identities = 36/120 (30%), Positives = 45/120 (37%), Gaps = 11/120 (9%)

		

		Query: 489 GGGGRNSAGNTGGAGGAGGGTNGIAGTTSGAGGGGGGGTQSAGGAGGTGGTNAGSAGSSL 548

		           GG GR   G+  GA    G  NG        G G GGG     G         G +GS +

		Sbjct: 3   GGDGR---GHNTGAHSTSGNINGGPT-----GLGVGGGASDGSGWSSENNPWGGGSGSGI 54

		

		Query: 549 TGGLGGDGRTSGTTDGSGASGGLASGGNGGGVIATSRAGGGGGGAGYFGGGGGSSSSSGS 608

		             G G      G   G+G SGG +  G     +A   A G    +    GG   S S+G+

		Sbjct: 55  HWGGGSGHGNGG---GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111

		

		

		

		 Score = 45.1 bits (106), Expect = 3e-06

		 Identities = 33/119 (27%), Positives = 46/119 (38%), Gaps = 4/119 (3%)

		

		Query: 464 GGGGGGYSSIYRGATPLAIAAGGGGGGGGRNSAGNTGGAGGAGGGTNGIAGTTSGAGGGG 523

		           GG G G+++   GA   +    GG  G G     + G    +     G  G+ SG   GG

		Sbjct: 3   GGDGRGHNT---GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGIHWGG 58

		

		Query: 524 GGGTQSAGGAGGTGGTNAGSAGSSLTGGLGGDGRTSGTTDGSGASGGLASGGNGGGVIA 582

		           G G  + GG G +GG +      S        G  + +T G+G      S G     IA

		Sbjct: 59  GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIA 117

		

		

		

		 Score = 44.3 bits (104), Expect = 5e-06

		 Identities = 30/95 (31%), Positives = 40/95 (42%), Gaps = 15/95 (15%)

		

		Query: 450 GGGGATGTRNTAGGGGGGGGYSSIYRGATPLAIAAGGGGGGGGRNSAGNTGGAGGAGGGT 509

		           G G  TG  +T+G   GG                 G G GGG  + +G +      GGG+

		Sbjct: 6   GRGHNTGAHSTSGNINGGPT---------------GLGVGGGASDGSGWSSENNPWGGGS 50

		

		Query: 510 NGIAGTTSGAGGGGGGGTQSAGGAGGTGGTNAGSA 544

		                   G+G G GGG  ++GG  GTGG  +  A

		Sbjct: 51  GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVA 85

		

		

		

		 Score = 41.2 bits (96), Expect = 4e-05

		 Identities = 29/86 (33%), Positives = 40/86 (46%), Gaps = 12/86 (13%)

		

		Query: 553 GGDGRTSGTTDGSGASGGLASGGNGGGVIATSRAGGGGGGAGY------FGGGGGSSSSS 606

		           GGDGR  G   G+ ++ G  +GG  G  +     GG   G+G+      +GGG GS    

		Sbjct: 3   GGDGR--GHNTGAHSTSGNINGGPTGLGVG----GGASDGSGWSSENNPWGGGSGSGIHW 56

		

		Query: 607 GSGAGGGGSGSSFVSGSLTTTAGGGQ 632

		           G G+G G  G +  SG  + T G   

		Sbjct: 57  GGGSGHGNGGGNGNSGGGSGTGGNLS 82

		

		

		

		 Score = 40.9 bits (95), Expect = 5e-05

		 Identities = 37/108 (34%), Positives = 43/108 (39%), Gaps = 22/108 (20%)

		

		Query: 568 SGGLASGGNGGGVIATSRAGGGGGGAGYFGGGGGSSSSSGSGA----GGGGSGSSFVSGS 623

		           SGG   G N G   A S +G   GG    G GGG+S  SG  +     GGGSGS    G 

		Sbjct: 2   SGGDGRGHNTG---AHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWG- 57

		

		Query: 624 LTTTAGGGQNPGNNTDTDRSGAGQGGNAGATGGTGTGGSNGIIIINYG 671

		                G G   G          G  GN+G   GTG   S     + +G

		Sbjct: 58  ----GGSGHGNG----------GGNGNSGGGSGTGGNLSAVAAPVAFG 91

		

		

		

		 Score = 40.1 bits (93), Expect = 9e-05

		 Identities = 33/115 (28%), Positives = 41/115 (35%), Gaps = 18/115 (15%)

		

		Query: 410 GGGGGGGAGGSAAAGGT---GGGGGYVSGSVAVTPGETLTVYVGGGGATGTRNTAGGGGG 466

		           GG G G   G+ +  G    G  G  V G  +   G +      GGG+    +  GG G 

		Sbjct: 3   GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62

		

		Query: 467 GGGYSSIYRGATPLAIAAGGGGGGGGRNSAGNTGGAGGAGGGTNGIAGTTSGAGG 521

		           G G               GG G  GG +  G    A  A       A +T GAGG

		Sbjct: 63  GNG---------------GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102

		

		

		

		 Score = 39.7 bits (92), Expect = 1e-04

		 Identities = 29/103 (28%), Positives = 44/103 (42%), Gaps = 3/103 (2%)

		

		Query: 485 GGGGGGGGRNSAGNTGGA---GGAGGGTNGIAGTTSGAGGGGGGGTQSAGGAGGTGGTNA 541

		           G G   G  +++GN  G     G GGG +  +G +S     GGG        GG+G  N 

		Sbjct: 6   GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65

		

		Query: 542 GSAGSSLTGGLGGDGRTSGTTDGSGASGGLASGGNGGGVIATS 584

		           G  G+S  G   G   ++     +     L++ G GG  ++ S

		Sbjct: 66  GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSIS 108

		

		

		

		 Score = 35.8 bits (82), Expect = 0.002

		 Identities = 26/79 (32%), Positives = 33/79 (41%)

		

		Query: 360 QGNWAGGTAVAGGYLYSVGGLSLNGQIYTAQGASTFTVPAGITSLSVKVWGGGGGGGAGG 419

		           +G+  G  + +G       GL + G      G S+   P G  S S   WGGG G G GG

		Sbjct: 7   RGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG 66

		

		Query: 420 SAAAGGTGGGGGYVSGSVA 438

		                G G G G    +VA

		Sbjct: 67  GNGNSGGGSGTGGNLSAVA 85
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS02235V8PROTEASE725e-16 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

		          Length = 336

		

		 Score = 72.0 bits (176), Expect = 5e-16

		 Identities = 40/186 (21%), Positives = 70/186 (37%), Gaps = 38/186 (20%)

		

		Query: 112 SQQSAGTGIIVSDNGYVVTNRHVVDADAT-KVSITLSDGSVLDDVTVVGR------TGSD 164

		           +     +G++V  +  ++TN+HVVDA      ++     ++  D    G       T   

		Sbjct: 99  TGTFIASGVVVGKD-TLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYS 157

		

		Query: 165 DSLDIAILKI-----NNTKGKSLHPATLGSSSSLQVGDKVVAIGNALG-----QFQNTVT 214

		              D+AI+K      N   G+ + PAT+ +++  QV   +   G          +++   

		Sbjct: 158 GEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGK 217

		

		Query: 215 AGILSGYGRSIEAGNEGGSQSELLQNLLQTDAAINSGNSGGPLVNMNSEVIGINTAVASG 274

		              L G                     +Q D +   GNSG P+ N  +EVIGI+      

		Sbjct: 218 ITYLKGEA-------------------MQYDLSTTGGNSGSPVFNEKNEVIGIHWG-GVP 257

		

		Query: 275 SAENIG 280

		           +  N  

		Sbjct: 258 NEFNGA 263
8L336_RS02350L336_RS02475Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS02350221-0.992315hypothetical protein
L336_RS02355222-1.491219RNA-binding protein
L336_RS02360121-2.577146heliorhodopsin HeR
L336_RS02365020-2.800083AAA family ATPase
L336_RS02370119-2.535995HAD hydrolase-like protein
L336_RS02375121-2.492117adenine phosphoribosyltransferase
L336_RS02380221-2.484712cyclase family protein
L336_RS02385221-2.320391hypothetical protein
L336_RS05610119-2.950574phosphoribosylanthranilate isomerase
L336_RS02395120-4.393367hypothetical protein
L336_RS02400123-5.564376NUDIX hydrolase
L336_RS02405122-6.056584AAA family ATPase
L336_RS02410426-6.336558hypothetical protein
L336_RS02415430-6.348205endonuclease/exonuclease/phosphatase family
L336_RS02420532-6.102429DUF4238 domain-containing protein
L336_RS05820632-5.739430hypothetical protein
L336_RS02425531-5.866505aldo/keto reductase
L336_RS02430633-6.122021DEAD/DEAH box helicase family protein
L336_RS02435634-6.146696site-specific DNA-methyltransferase
L336_RS02440737-5.732592helix-turn-helix domain-containing protein
L336_RS02445637-5.489634helix-turn-helix domain-containing protein
L336_RS02450328-3.573387hypothetical protein
L336_RS02455226-3.133231hypothetical protein
L336_RS05615323-2.992869septation protein SpoVG family protein
L336_RS02460221-2.907579hypothetical protein
L336_RS02465316-1.910540recombinase family protein
L336_RS05620514-0.750288Ig-like domain=containing protein
L336_RS02475418-1.369432hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS05620SURFACELAYER434e-06 Lactobacillus surface layer protein signature.
		>SURFACELAYER#Lactobacillus surface layer protein signature.

		          Length = 439

		

		 Score = 43.1 bits (101), Expect = 4e-06

		 Identities = 47/221 (21%), Positives = 80/221 (36%), Gaps = 35/221 (15%)

		

		Query: 490 PDASPALTVTASQTDAAGNTGNAGPQTALKDTVAPTASITPLLSNSGSPALSGAVSDPSA 549

		           P A+ A+ V A+ T  A +  NA   T  K  V  T SI+ + + + S  +       + 

		Sbjct: 20  PIAATAMPVNAATTINADSAINA--NTNAKYDVDVTPSISAIAAVAKSDTMPAIPGSLTG 77

		

		Query: 550 TVTVTVNGTTYTAT---NNGDGTWSLPAGT---IAPALADGPYDIVITATDGVG-NTGTD 602

		           +++ + NG +YTA    ++G+ T +         A   AD  Y + +     V  N G++

		Sbjct: 78  SISASYNGKSYTANLPKDSGNATITDSNNNTVKPAELEADKAYTVTVP---DVSFNFGSE 134

		

		Query: 603 STADELTI-----DKTAPTGSLAPVAPGITNSPALSGTVSDPSAIVTVTVDGTTYTATNN 657

		           +   E+TI     + T    +    A  +  +    G     S  +    +      T N

		Sbjct: 135 NAGKEITIGSANPNVTFTEKTGDQPASTVKVTLDQDGVAKLSSVQIK---NVYAIDTTYN 191

		

		Query: 658 GDGTWSLPAGTIAPALNPGTYDVVVSFTDTAGNTSTDPTTN 698

		            +  +               YDV    T T G  S D    

		Sbjct: 192 SNVNF---------------YDVTTGATVTTGAVSIDADNQ 217
9L336_RS05825L336_RS02675Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS05825216-3.626861hypothetical protein
L336_RS02565219-4.403669hypothetical protein
L336_RS05635220-5.149128FG-GAP repeat protein
L336_RS02575322-6.566977UDP-N-acetylglucosamine 2-epimerase
L336_RS02580222-6.582204methyltransferase domain-containing protein
L336_RS02585222-6.530686hypothetical protein
L336_RS02590220-5.368812glycosyltransferase
L336_RS05640115-4.375571ABC transporter ATP-binding protein
L336_RS02600-115-3.872171ABC transporter permease
L336_RS02605-115-2.675658glycosyltransferase family 4 protein
L336_RS02610-113-2.578219succinyl-CoA synthetase (beta subunit)
L336_RS02615014-2.635821succinate--CoA ligase subunit alpha
L336_RS02620013-3.176618glycosyltransferase family 2 protein
L336_RS02625015-4.125195GtrA family protein
L336_RS02630015-4.033509adenylyltransferase/cytidyltransferase family
L336_RS02635116-3.810635UDP-N-acetylglucosamine 2-epimerase
L336_RS02640016-4.692068glycosyltransferase family 4 protein
L336_RS02645015-4.409318KH domain-containing protein
L336_RS02650018-5.083868membrane protein insertase YidC
L336_RS02655021-4.912130ribonuclease P protein component
L336_RS02660020-5.36406650S ribosomal protein L34
L336_RS02665019-4.795369chromosomal replication initiator protein DnaA
L336_RS02670221-4.425377DNA polymerase III subunit beta
L336_RS02675118-3.685663DNA replication and repair protein RecF
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS02580PF01540300.024 Adhesin lipoprotein
		>PF01540#Adhesin lipoprotein

		          Length = 475

		

		 Score = 30.5 bits (68), Expect = 0.024

		 Identities = 23/87 (26%), Positives = 45/87 (51%), Gaps = 5/87 (5%)

		

		Query: 220 EEIPSAEQAVQGVASYSMNY---LHELQGAIESVITKKIAQDGEIERLQRENSTQKEQLD 276

		           +EI  A ++ +   SY  +Y   + +L  A+E+  +++   D   +++  EN   KE   

		Sbjct: 70  KEIAEATKSFKEAGSYG-DYPAIISKLSAAVENAKSEQQKVDQANKKIADENLKIKEGAK 128

		

		Query: 277 ELWRITNSKRQRVANKLANTVGKVMPK 303

		           EL +++  K Q  A+ +A T+ K+  K

		Sbjct: 129 ELLKLSE-KIQSFADTIALTITKLEGK 154
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS02620TYPE3IMPPROT280.049 Type III secretion system inner membrane P protein ...
		>TYPE3IMPPROT#Type III secretion system inner membrane P protein

		           family signature.

		          Length = 224

		

		 Score = 27.8 bits (62), Expect = 0.049

		 Identities = 15/54 (27%), Positives = 24/54 (44%), Gaps = 5/54 (9%)

		

		Query: 221 ITSLTTAPLRISTYVGAFTSFVAFVYIVYLLVRPLFGVPTVPGYASTLAVILFL 274

		           +   T  P      + + T FV F  IV+++VR   G+  +P   +   V L L

		Sbjct: 11  LAFSTLLPF----IIASGTCFVKFS-IVFVMVRNALGLQQIPSNMTLNGVALLL 59
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS02630LPSBIOSNTHSS404e-07 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

		          signature.

		          Length = 166

		

		 Score = 40.2 bits (94), Expect = 4e-07

		 Identities = 16/57 (28%), Positives = 26/57 (45%), Gaps = 5/57 (8%)

		

		Query: 4  VIISGYFSPLHGGHLDLIEGAKALGDRLIVIVNNDKQQLIKKGKIVLNENERYRIMK 60

		           I  G F P+  GHLD+IE    L D++ V V  +  +     + + +  ER   + 

		Sbjct: 3  AIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNK-----QPMFSVQERLEQIA 54
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS0265060KDINNERMP1222e-33 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

		          Length = 548

		

		 Score =  122 bits (307), Expect = 2e-33

		 Identities = 64/258 (24%), Positives = 105/258 (40%), Gaps = 60/258 (23%)

		

		Query: 8   IVKPILNALVLLYSIVPGGDFGVAIILFTILIRTLMYPLVRSQLHQSRTMHKLQPELAKI 67

		           I +P+   L  ++S V  G++G +II+ T ++R +MYPL ++Q      M  LQP++  +

		Sbjct: 336 ISQPLFKLLKWIHSFV--GNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQAM 393

		

		Query: 68  KARAAGDKQQEASQMMDLYKRYGISPFRSILILIIQLPIFIGLYQVIQVFTLHINQLAHY 127

		           + R   DKQ+ + +MM LYK   ++P      L+IQ+PIF+ LY ++      +  +   

		Sbjct: 394 RERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYML------MGSVELR 447

		

		Query: 128 TYPFVQQIPSVKAIIDTPASFNNTMLGVIDLTKTTFGNHGVDYILLLLAFISAATQYILT 187

		             PF   I  + A                             Y   +L  +   T + + 

		Sbjct: 448 QAPFALWIHDLSA--------------------------QDPY--YILPILMGVTMFFIQ 479

		

		Query: 188 KQTMPQSTVKKKFRDIVAEAAEGKSTNQTDMNAAMMSGMAKFMPAMMFLIMISLPGALAL 247

		           K +                      T  TD        +  FMP +  +  +  P  L L

		Sbjct: 480 KMS---------------------PTTVTDPMQQK---IMTFMPVIFTVFFLWFPSGLVL 515

		

		Query: 248 YYTVSNLVAVAQQHYLLS 265

		           YY VSNLV + QQ  +  

		Sbjct: 516 YYIVSNLVTIIQQQLIYR 533
10L336_RS02850L336_RS05655Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS02850-1133.205994tRNA
L336_RS02855-1112.913543UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
L336_RS02860-2143.094859hypothetical protein
L336_RS02865-2132.681282peptidoglycan bridge formation glycyltransferase
L336_RS02870-2174.169337hypothetical protein
L336_RS05650-2245.604709hypothetical protein
L336_RS02885-1254.118234glucose-6-phosphate isomerase
L336_RS028900324.240074ABC transporter permease
L336_RS028950252.730257ABC transporter ATP-binding protein
L336_RS056550273.073754*L,D-transpeptidase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS02855NUCEPIMERASE290.032 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

		          Length = 334

		

		 Score = 29.0 bits (65), Expect = 0.032

		 Identities = 18/65 (27%), Positives = 23/65 (35%), Gaps = 9/65 (13%)

		

		Query: 375 TEIDDRREAISKALGVATKGDTILITGMG--------HEVYRIIGGKRVPWND-AAVVRE 425

		           T IDD  EAI +   V    DT      G        + VY I     V   D    + +

		Sbjct: 218 TYIDDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALED 277

		

		Query: 426 LLGKK 430

		            LG +

		Sbjct: 278 ALGIE 282
11L336_RS03065L336_RS03140Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS030652121.369834hypothetical protein
L336_RS030703140.681984hypothetical protein
L336_RS056702160.747609HAD family phosphatase
L336_RS030804151.371313hypothetical protein
L336_RS030854152.209055hypothetical protein
L336_RS030903151.830572hypothetical protein
L336_RS030954111.927523LamG domain-containing protein
L336_RS031005111.170710hypothetical protein
L336_RS031055121.611910hypothetical protein
L336_RS031104121.420304hypothetical protein
L336_RS031153110.655699hypothetical protein
L336_RS056752110.814842hypothetical protein
L336_RS03125-1120.836960hypothetical protein
L336_RS031300112.119892hypothetical protein
L336_RS031351121.604019bifunctional DNA-formamidopyrimidine
L336_RS031402111.522442hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03070BCTERIALGSPG270.028 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

		          signature.

		          Length = 145

		

		 Score = 27.2 bits (60), Expect = 0.028

		 Identities = 17/86 (19%), Positives = 36/86 (41%), Gaps = 10/86 (11%)

		

		Query: 1  MKRMYRGSGPKIFPIIVVVIVIALLIAALVTVGRMLFAGNS-----TTQVPQVTSSIRNE 55

		          M+   +  G  +  I+VV+++I +L A+LV    M     +      + +  + +++   

		Sbjct: 1  MRATDKQRGFTLLEIMVVIVIIGVL-ASLVVPNLMGNKEKADKQKAVSDIVALENALDMY 59

		

		Query: 56 VLDTG----TTRGVRYTVRGPIVADE 77

		           LD      T +G+   V  P +   

		Sbjct: 60 KLDNHHYPTTNQGLESLVEAPTLPPL 85
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03125VACJLIPOPROT290.040 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

		          Length = 251

		

		 Score = 29.5 bits (66), Expect = 0.040

		 Identities = 16/83 (19%), Positives = 30/83 (36%), Gaps = 10/83 (12%)

		

		Query: 650 TLYVDNMQIVVYYTVPDIIKFYDNVTPTNAAS-----ISSIGSDPT---NGVRGTIYQSY 701

		           T+Y  N  ++  Y V  +   + +  P  A +       ++          ++G  YQ  

		Sbjct: 38  TMYNFNFNVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGM 97

		

		Query: 702 QEVNLFSASTVVAAGSDGLWDFA 724

		                F  +T++  G  G  D A

		Sbjct: 98  VHFTRFFLNTILGMG--GFIDVA 118
12L336_RS03245L336_RS03285Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS03245214-1.163936glycoside hydrolase
L336_RS03250013-1.004540ACT domain-containing protein
L336_RS05930117-1.44805830S ribosomal protein S6
L336_RS03260018-0.806985single-stranded DNA-binding protein
L336_RS032654275.53788930S ribosomal protein S18
L336_RS059353306.524006hypothetical protein
L336_RS032702255.247800diaminopimelate decarboxylase
L336_RS032751306.057771elongation factor P
L336_RS032802285.927480TlyA family RNA methyltransferase
L336_RS032852194.677544hypothetical protein
13L336_RS04180L336_RS04260Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS041801273.611657type IV secretion system DNA-binding
L336_RS041851274.555083hypothetical protein
L336_RS041900223.593923hypothetical protein
L336_RS04195-1213.715664hypothetical protein
L336_RS042000182.911008type I DNA topoisomerase
L336_RS042050182.797858hypothetical protein
L336_RS042100161.618537DNA-processing protein DprA
L336_RS042150150.124314ZIP family metal transporter
L336_RS04220-1200.509586aminoacyl-tRNA hydrolase
L336_RS04225-121-0.186574hypothetical protein
L336_RS04230-1220.658705hypothetical protein
L336_RS042351294.156356sortase
L336_RS042401253.488120reverse transcriptase/maturase family protein
L336_RS042453273.542774hypothetical protein
L336_RS042504273.776232hypothetical protein
L336_RS042553263.908815four helix bundle protein
L336_RS042601223.636955hypothetical protein
14L336_RS04405L336_RS04595Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS04405-1183.182694hypothetical protein
L336_RS04410-2162.849577PEGA domain-containing protein
L336_RS04415-1183.363093sortase
L336_RS04425-2194.071907*hypothetical protein
L336_RS04430-1173.070413hypothetical protein
L336_RS04435-2172.418650HAD family phosphatase
L336_RS04440-3161.4052818-oxo-dGTP diphosphatase
L336_RS04445-3182.271407hypothetical protein
L336_RS04450-3203.534844CapA family protein
L336_RS04455-2376.444166M15 family metallopeptidase
L336_RS059550376.983624hypothetical protein
L336_RS05725-1315.765749DUF2730 family protein
L336_RS044650315.957515exodeoxyribonuclease III
L336_RS044701326.220116hypothetical protein
L336_RS044751275.060879SPFH/Band 7/PHB domain protein
L336_RS044801234.296569UMP kinase
L336_RS044852366.965253glycosyltransferase
L336_RS044902427.678725glycosyltransferase family 2 protein
L336_RS0596025410.567605hypothetical protein
L336_RS045003439.261521SsrA-binding protein SmpB
L336_RS045053336.509018hypothetical protein
L336_RS045103294.745261hypothetical protein
L336_RS05730-1191.510390hypothetical protein
L336_RS04520-1171.817460hypothetical protein
L336_RS04525-1151.570579hypothetical protein
L336_RS04530-1170.938202ATP-binding protein
L336_RS045352211.176848NrdH-redoxin
L336_RS045400200.670239hypothetical protein
L336_RS045451201.722140hypothetical protein
L336_RS045502172.948672AtpZ/AtpI family protein
L336_RS045551152.560094F0F1 ATP synthase subunit A
L336_RS045602172.732919ATP synthase F0 subunit C
L336_RS045652182.794049F0F1 ATP synthase subunit B
L336_RS057351214.015601F0F1 ATP synthase subunit delta
L336_RS045751214.751985F0F1 ATP synthase subunit alpha
L336_RS058400214.838162hypothetical protein
L336_RS045850214.816252ATP synthase F1 subunit gamma
L336_RS04590-1174.656057hypothetical protein
L336_RS04595-2153.282439F0F1 ATP synthase subunit beta
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS04405ACRIFLAVINRP310.006 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

		          Length = 1034

		

		 Score = 31.3 bits (71), Expect = 0.006

		 Identities = 12/49 (24%), Positives = 22/49 (44%), Gaps = 2/49 (4%)

		

		Query: 157 RRVVSVLSGSLALLLLGAYVTYLNMP-SLSTRVAAAQAGINATYPGYQP 204

		           RR +     ++ L++ GA    L +P +    +A     ++A YPG   

		Sbjct: 7   RRPIFAWVLAIILMMAGAL-AILQLPVAQYPTIAPPAVSVSANYPGADA 54
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS05725OMADHESIN280.017 Yersinia outer membrane adhesin signature.
		>OMADHESIN#Yersinia outer membrane adhesin signature.

		          Length = 455

		

		 Score = 27.6 bits (60), Expect = 0.017

		 Identities = 9/20 (45%), Positives = 14/20 (70%)

		

		Query: 44  DKRFEHIDSRFEKIDKRFDK 63

		           D +F  +D+R +K+D R DK

		Sbjct: 365 DHKFRQLDNRLDKLDTRVDK 384
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS04480CARBMTKINASE300.006 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

		          Length = 314

		

		 Score = 30.2 bits (68), Expect = 0.006

		 Identities = 15/59 (25%), Positives = 25/59 (42%), Gaps = 13/59 (22%)

		

		Query: 119 LDKHRVVIVAGGTGRPFLTT-------------DTAAVNLALELQCDVVVKTTKVDGVY 164

		           +++  +VI +GG G P +               D A   LA E+  D+ +  T V+G  

		Sbjct: 183 VERGVIVIASGGGGVPVILEDGEIKGVEAVIDKDLAGEKLAEEVNADIFMILTDVNGAA 241
15L336_RS04695L336_RS04805Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS04695018-3.297132rRNA adenine N(6)-methyltransferase family
L336_RS04700120-3.631564glycosyltransferase family 4 protein
L336_RS04705225-5.430924hypothetical protein
L336_RS04710327-5.213858CPBP family intramembrane metalloprotease
L336_RS04715219-0.828793CPBP family intramembrane metalloprotease
L336_RS047200160.783302hypothetical protein
L336_RS057500141.684457*hypothetical protein
L336_RS058450132.033210hypothetical protein
L336_RS047300131.604319SulP family inorganic anion transporter
L336_RS04735-1121.905772hypothetical protein
L336_RS04745-2111.181314hypothetical protein
L336_RS04755-1152.942677AI-2E family transporter
L336_RS04760-2173.413322MFS transporter
L336_RS047650202.769824cysteine--tRNA ligase
L336_RS047701233.150642DHH family phosphoesterase
L336_RS047751222.833869hypothetical protein
L336_RS047801212.199878recombination mediator RecR
L336_RS047851202.141822YbaB/EbfC family nucleoid-associated protein
L336_RS047901202.390620glycosyltransferase family 39 protein
L336_RS047951203.226431replicative DNA helicase
L336_RS057551203.444227DNA polymerase III subunit gamma/tau
L336_RS048052233.688056thymidine kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS04705BCTERIALGSPH290.008 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

		          signature.

		          Length = 170

		

		 Score = 29.1 bits (65), Expect = 0.008

		 Identities = 9/44 (20%), Positives = 25/44 (56%)

		

		Query: 6  KGFGVVEIVLVVVVVGLIGVLGWMFFNKQKETKTSDNTASNSAQ 49

		          +GF ++E++L+++++G+   +  + F   ++   +   A   AQ

		Sbjct: 4  RGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQ 47
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS04760TCRTETA461e-07 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

		          Length = 399

		

		 Score = 46.0 bits (109), Expect = 1e-07

		 Identities = 55/279 (19%), Positives = 100/279 (35%), Gaps = 29/279 (10%)

		

		Query: 60  GIIADRIGRKRALITASLFQLLSLCILAISPSLLVYGIGAAFYALYTAFQNGAFQAFLYD 119

		           G ++DR GR+  L+ +     +   I+A +P L V  IG     +  A    A  A++ D

		Sbjct: 64  GALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVA-GAYIAD 122

		

		Query: 120 HLLSEGRERRYARYMGQASALFLLGAGVANALSGIIAEYSNLRVPYLLSILPALVALATI 179

		               + R    AR+ G  SA F  G      L G++  +S     +  + L  L  L   

		Sbjct: 123 ITDGDER----ARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGC 178

		

		Query: 180 MTLREPSRHKESELSGWRAHAKDIITVIASRPIIWVLGLIFFAANAFWLTVGEFGQVYIL 239

		             L E     + E    R  A + +        + V+  +        L       ++++

		Sbjct: 179 FLLPES---HKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVI 235

		

		Query: 240 ----SFGVSAAVLGLLWALTALISGLAQ------FTSHWVKNRIAHISFVF---IIFLVA 286

		                F   A  +G+  A   ++  LAQ        +   + R   +  +       L+A

		Sbjct: 236 FGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLA 295

		

		Query: 287 FALIQSWVGIGLFIVIY-------AITGLLETIADAAIQ 318

		           FA  + W+   + +++        A+  +L    D   Q

		Sbjct: 296 FA-TRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQ 333
16L336_RS04845L336_RS04940Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS048451173.403991hypothetical protein
L336_RS048501213.102310hypothetical protein
L336_RS048552203.378449hypothetical protein
L336_RS048600204.108979dihydrofolate reductase
L336_RS04865-2203.846619thymidylate synthase
L336_RS048701246.292749hypothetical protein
L336_RS048750245.844974HIT domain-containing protein
L336_RS048800256.628616SDR family NAD(P)-dependent oxidoreductase
L336_RS048851246.31308050S ribosomal protein L1
L336_RS048902287.444725hypothetical protein
L336_RS0489534010.194700hypothetical protein
L336_RS049003369.001086hypothetical protein
L336_RS049053368.872190hypothetical protein
L336_RS057602368.476986M15 family metallopeptidase
L336_RS049152358.793960hypothetical protein
L336_RS049201266.832680hypothetical protein
L336_RS057652215.516004*phosphatidate cytidylyltransferase
L336_RS049352183.905652hypothetical protein
L336_RS049402172.79163450S ribosomal protein L11
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS04880DHBDHDRGNASE661e-14 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

		           signature.

		          Length = 261

		

		 Score = 66.2 bits (161), Expect = 1e-14

		 Identities = 52/239 (21%), Positives = 95/239 (39%), Gaps = 20/239 (8%)

		

		Query: 19  VVISGARRGIGHAVADRFLQSYNTAVIGVDNHPD---IVEQFPERQGRNFWPVQLDICDT 75

		             I+GA +GIG AVA          +  VD +P+    V    + + R+      D+ D+

		Sbjct: 11  AFITGAAQGIGEAVARTLASQ-GAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDS 69

		

		Query: 76  DGVKELFGEVIAAPQPLDAVVHAAGHIVAGTDYHRHVRDYPTETARQQVKRLREVNGQAA 135

		             + E+   +     P+D +V+ AG +  G  +           + ++ +    VN    

		Sbjct: 70  AAIDEITARIEREMGPIDILVNVAGVLRPGLIH---------SLSDEEWEATFSVNSTGV 120

		

		Query: 136 IHFVSQAVGALSAQENGGCVIGISSSKADFPDPYRKEYMLAKARF-SEAMAIQRTKLPPS 194

		            +        +  + +G  V  + S+ A  P      Y  +KA        +       +

		Sbjct: 121 FNASRSVSKYMMDRRSGSIVT-VGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYN 179

		

		Query: 195 IRLIDVQPGNTQTDIDGGEWIDGSDPDTARAVQAVNEWWRTHFGTPVKTIAEAIYDIAQ 253

		           IR   V PG+T+TD+    W D  +    + ++   E ++T  G P+K +A+   DIA 

		Sbjct: 180 IRCNIVSPGSTETDMQWSLWAD--ENGAEQVIKGSLETFKT--GIPLKKLAKP-SDIAD 233
17L336_RS01445L336_RS01480N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS01445-2235.333850VIT family protein
L336_RS01455-2235.311023*hypothetical protein
L336_RS01460-1184.011106hypothetical protein
L336_RS01465-1120.284726DUF11 domain-containing protein
L336_RS014700130.160348HAMP domain-containing histidine kinase
L336_RS01475112-1.178328hypothetical protein
L336_RS01480113-1.924984response regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS01445RTXTOXIND290.022 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

		           signature.

		          Length = 478

		

		 Score = 28.6 bits (64), Expect = 0.022

		 Identities = 16/97 (16%), Positives = 36/97 (37%), Gaps = 5/97 (5%)

		

		Query: 74  EYVSVSSQSDAEKAYIELEKADLKDNPEDELDELAREYQKLGLSKQTSHRVAAELTEKNA 133

		           +YV   ++    K+ +E  ++++  + ++E   + + ++   L K         L     

		Sbjct: 260 KYVEAVNELRVYKSQLEQIESEI-LSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLEL 318

		

		Query: 134 LKAHLHVHFNLDPEDINSPMHAAIASLLAFTAGGLVP 170

		            K             I +P+   +  L   T GG+V 

		Sbjct: 319 AKNE----ERQQASVIRAPVSVKVQQLKVHTEGGVVT 351
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS01460PF03544361e-04 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

		          Length = 243

		

		 Score = 36.5 bits (84), Expect = 1e-04

		 Identities = 17/47 (36%), Positives = 19/47 (40%), Gaps = 2/47 (4%)

		

		Query: 404 ANPGTDESVAPTDAGQPPAVYTPPAVPAPPVVDTPPAPTPEQQAPKP 450

		           A P +   VAP D   P AV  PP     P  +  P P PE     P

		Sbjct: 47  AQPISVTMVAPADLEPPQAVQPPPEPVVEP--EPEPEPIPEPPKEAP 91
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS01470PF06580310.018 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

		          Length = 349

		

		 Score = 31.0 bits (70), Expect = 0.018

		 Identities = 17/103 (16%), Positives = 36/103 (34%), Gaps = 25/103 (24%)

		

		Query: 585 VIMNFIDNAIYY----SPEGSTIAIRLKVEAGAAVLTVKDSGMGVPKSEQKHLFTKFFRA 640

		           ++   ++N I +     P+G  I ++   + G   L V+++G    K+            

		Sbjct: 259 LVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNT----------- 307

		

		Query: 641 ENARKQRPDGTGIGLFLAKKVVDAHGG---ALVFESLPGKGST 680

		                   + TG GL   ++ +    G    +      GK + 

		Sbjct: 308 -------KESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNA 343
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS01480HTHFIS741e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

		          Length = 484

		

		 Score = 73.7 bits (181), Expect = 1e-18

		 Identities = 21/103 (20%), Positives = 40/103 (38%), Gaps = 2/103 (1%)

		

		Query: 2   TKIAIIEDDQVINQMYRMKFEAAGFEVSTAGDGETGVALVKKVTPDIILLDLQMPHMNGA 61

		             I + +DD  I  +       AG++V    +  T    +     D+++ D+ MP  N  

		Sbjct: 4   ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

		

		Query: 62  EALTAIRGNAASSKTPVIILTNLGEEEAPKNLRSLGIHSYIVK 104

		           + L  I+   A    PV++++              G + Y+ K

		Sbjct: 64  DLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPK 104
18L336_RS01525L336_RS05540N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS01525018-1.497382type II secretion system GspH family protein
L336_RS01535420-2.915119hypothetical protein
L336_RS01540217-2.230085type II secretion system GspH family protein
L336_RS05535115-2.103128prepilin-type N-terminal cleavage/methylation
L336_RS05540114-2.063761prepilin-type N-terminal cleavage/methylation
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS01530BCTERIALGSPG466e-09 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

		          signature.

		          Length = 145

		

		 Score = 46.0 bits (109), Expect = 6e-09

		 Identities = 16/72 (22%), Positives = 35/72 (48%), Gaps = 8/72 (11%)

		

		Query: 1  MAERRGFTIVELVIVMVIMAILLTLTISGVTSGQVSARDSERKADAENIARGLERYYNEV 60

		            ++RGFT++E+++V+VI+ +L +L +  +   +  A   +  +D   +   L+ Y  + 

		Sbjct: 4  TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLD- 62

		

		Query: 61 AKPSVGRTGRYP 72

		                    YP

		Sbjct: 63 -------NHHYP 67
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS01540BCTERIALGSPG334e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

		          signature.

		          Length = 145

		

		 Score = 32.9 bits (75), Expect = 4e-04

		 Identities = 22/88 (25%), Positives = 37/88 (42%), Gaps = 12/88 (13%)

		

		Query: 5  KTASQPGFTLVEMLVVAPIVILVVGGIVALLIALVGDVLIARERNSMAYNTQDALNLIEQ 64

		           T  Q GFTL+E++VV  IVI+       +L +LV   L+  +  +        +  +E 

		Sbjct: 3  ATDKQRGFTLLEIMVV--IVII------GVLASLVVPNLMGNKEKADKQKAVSDIVALEN 54

		

		Query: 65 DVRLSSNIQATTGTLPSP-QGSDSNVSG 91

		           +      +      P+  QG +S V  

		Sbjct: 55 AL---DMYKLDNHHYPTTNQGLESLVEA 79
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS05535BCTERIALGSPG462e-09 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

		          signature.

		          Length = 145

		

		 Score = 46.4 bits (110), Expect = 2e-09

		 Identities = 18/70 (25%), Positives = 35/70 (50%)

		

		Query: 4  KNRGFTIVELLIVIVIIAILAAITIVAYNGIQTRAKASAVQATVNTVIKKAESANAVANS 63

		          K RGFT++E+++VIVII +LA++ +    G + +A      + +  +    +      + 

		Sbjct: 6  KQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLDNHH 65

		

		Query: 64 YPQNAAGFTA 73

		          YP    G  +

		Sbjct: 66 YPTTNQGLES 75
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS05540BCTERIALGSPG514e-11 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

		          signature.

		          Length = 145

		

		 Score = 51.4 bits (123), Expect = 4e-11

		 Identities = 18/65 (27%), Positives = 35/65 (53%)

		

		Query: 1  MKKSSGFTIVELLIVIVVIAILATITIVVYGSVQNRARSSAGQSQVNHLAKKVESFNTVN 60

		            K  GFT++E+++VIV+I +LA++ +      + +A      S +  L   ++ +   N

		Sbjct: 4  TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLDN 63

		

		Query: 61 NTYPT 65

		          + YPT

		Sbjct: 64 HHYPT 68
19L336_RS03345L336_RS03370N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS03345-117-1.006824type II secretion system F family protein
L336_RS03350016-0.037605type II secretion system GspH family protein
L336_RS03355115-0.711266A24 family peptidase
L336_RS03360115-1.461099hypothetical protein
L336_RS03365115-2.204693exported protein of unknown function
L336_RS03370017-3.110834prepilin-type N-terminal cleavage/methylation
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03345BCTERIALGSPF302e-102 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

		           signature.

		          Length = 408

		

		 Score =  302 bits (774), Expect = e-102

		 Identities = 128/405 (31%), Positives = 213/405 (52%), Gaps = 7/405 (1%)

		

		Query: 1   MKKFNYEARDKNSDKIIKSIVQADSETSAAKALVEQGYTPLTIKEVDE------NGGLIG 54

		           M +++Y+A D    K  +   +ADS   A + L E+G  PL++ E         + GL  

		Sbjct: 1   MAQYHYQALDAQGKKC-RGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSL 59

		

		Query: 55  RLTGRIKTKDKIVFTRQLATLIGAGLPLAQSIRTVLDQTQNKKLQGVIQEVIADVEGGKQ 114

		           R   R+ T D  + TRQLATL+ A +PL +++  V  Q++   L  ++  V + V  G  

		Sbjct: 60  RRKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHS 119

		

		Query: 115 LSEAFGKHPEVFDKIFLALVAAGEVSGTLDEALKRVAAQQEKDAAMMSKIKGAMTYPIIV 174

		           L++A    P  F++++ A+VAAGE SG LD  L R+A   E+   M S+I+ AM YP ++

		Sbjct: 120 LADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVL 179

		

		Query: 175 MVVILAVMLFMLFTVVPQVEKLYHDMKKSLPMLTEIMINSANFMIHFWWLVLVVMFIGIY 234

		            VV +AV+  +L  VVP+V + +  MK++LP+ T +++  ++ +  F   +L+ +  G  

		Sbjct: 180 TVVAIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFM 239

		

		Query: 235 FLRQYMHTENGIRTFDTLKLNAPLFKGMFRKLYMARFTRTGQTLLSTGVAMLDMLRISSE 294

		             R  +  E    +F    L+ PL   + R L  AR+ RT   L ++ V +L  +RIS +

		Sbjct: 240 AFRVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGD 299

		

		Query: 295 SVNNVVVSKSIDRAAEKVKGGKALSAAIQSEEYILPMVPQMIKIGEQSGKIDEMMGKTAQ 354

		            ++N      +  A + V+ G +L  A++      PM+  MI  GE+SG++D M+ + A 

		Sbjct: 300 VMSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAAD 359

		

		Query: 355 VFEDELDEEIKAISTAIEPILMVVLAIVAGGMVGAILFPIYSLVN 399

		             + E   ++       EP+L+V +A V   +V AIL PI  L  

		Sbjct: 360 NQDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNT 404
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03350BCTERIALGSPH387e-06 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

		          signature.

		          Length = 170

		

		 Score = 37.6 bits (87), Expect = 7e-06

		 Identities = 13/30 (43%), Positives = 22/30 (73%)

		

		Query: 6  KQRGFTIIEVVLVLAIAALIFLMVFIALPA 35

		          +QRGFT++E++L+L +  +   MV +A PA

		Sbjct: 2  RQRGFTLLEMMLILLLMGVSAGMVLLAFPA 31
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03355PREPILNPTASE1354e-40 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

		           signature.

		          Length = 290

		

		 Score =  135 bits (341), Expect = 4e-40

		 Identities = 83/283 (29%), Positives = 132/283 (46%), Gaps = 16/283 (5%)

		

		Query: 8   LIVSIGGLMLGSFAGAQVWRLRARQLDEDRQAGETIDKQELSRLKPLISVSLTSDRSVCL 67

		            +V +  LM+GSF    + RL      E +    +    +   +      +L   RS C 

		Sbjct: 17  SLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFNPDDEGV-DEPPYNLMVPRSCCP 75

		

		Query: 68  YCHHQLRWYDMIPLLSWAGTGGRCRYCRQSIGSLEPLIELGLSAAFVISYLYWPTPLTSG 127

		           +C+H +   + IPLLSW    GRCR C+  I +  PL+EL  +   V   +         

		Sbjct: 76  HCNHPITALENIPLLSWLWLRGRCRGCQAPISARYPLVELLTALLSVAVAMTL------A 129

		

		Query: 128 VAWGAFVVWLLSLTSLAILVTYDIRWQLLPDIVSYCYMLLGIIFIVLGFHGGWGSIVE-V 186

		             WG     LL+   L  L   D+   LLPD ++   +  G++F      GG+ S+ + V

		Sbjct: 130 PGWGTLAALLLTWV-LVALTFIDLDKMLLPDQLTLPLLWGGLLF---NLLGGFVSLGDAV 185

		

		Query: 187 VGAVA---VLGGLYGILYMLSGGRWIGFGDVKLGVGLGLFLSNWRQALLALFLANLIGCI 243

		           +GA+A   VL  LY    +L+G   +G+GD KL   LG +L  W+   + L L++L+G  

		Sbjct: 186 IGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLG-WQALPIVLLLSSLVGAF 244

		

		Query: 244 VVIPGLISRKLTSTSHIPFGPFLVAGMMLAFFWGEWLISRLLS 286

		           + I  ++ R    +  IPFGP+L     +A  WG+ +    L+

		Sbjct: 245 MGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWYLT 287
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03370BCTERIALGSPG280.021 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

		          signature.

		          Length = 145

		

		 Score = 27.9 bits (62), Expect = 0.021

		 Identities = 11/45 (24%), Positives = 24/45 (53%), Gaps = 3/45 (6%)

		

		Query: 9  KGFTLIELMISMTFLSILLIAITVATLQIMHQYSKGMTVKSINQI 53

		          +GFTL+E+M+ +  + +L    ++    +M    K    K+++ I

		Sbjct: 8  RGFTLLEIMVVIVIIGVLA---SLVVPNLMGNKEKADKQKAVSDI 49
20L336_RS03595L336_RS03640N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS03595-214-0.723742hypothetical protein
L336_RS03600-216-0.652223ROK family protein
L336_RS03605017-0.740643LPXTG cell wall anchor domain-containing
L336_RS03610-216-0.144543S1 RNA-binding domain-containing protein
L336_RS03615-2160.088005translation initiation factor IF-2
L336_RS03620-1170.254566hypothetical protein
L336_RS03625-1150.279401hypothetical protein
L336_RS03635014-0.002391hypothetical protein
L336_RS03640-213-0.307765hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03595PREPILNPTASE290.007 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

		           signature.

		          Length = 290

		

		 Score = 29.0 bits (65), Expect = 0.007

		 Identities = 17/75 (22%), Positives = 26/75 (34%), Gaps = 18/75 (24%)

		

		Query: 70  LEMERAAWDKARELADMYQIIIDDSDIEESLDTYRDWLHSRSLCPHCNSTGLQAASN--- 126

		           + +ER    + R   +     +D+           + +  RS CPHCN   + A  N   

		Sbjct: 39  IMLEREWQAEYRSYFNPDDEGVDEPPY--------NLMVPRSCCPHCNHP-ITALENIPL 89

		

		Query: 127 ------HYRCAACQH 135

		                   RC  CQ 

		Sbjct: 90  LSWLWLRGRCRGCQA 104
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03600PF03309346e-04 Bvg accessory factor
		>PF03309#Bvg accessory factor

		          Length = 271

		

		 Score = 33.6 bits (77), Expect = 6e-04

		 Identities = 28/165 (16%), Positives = 55/165 (33%), Gaps = 22/165 (13%)

		

		Query: 1   MLITIDTGGTKTLVASFGSD---GKMGESIKYPT--PADPKEYATTLKNVVREQYGQKKV 55

		           ML+ ID   T T+V          K+ +  +  T       E A T+  ++ +    +++

		Sbjct: 1   MLLAIDVRNTHTVVGLISGSGDHAKVVQQWRIRTEPEVTADELALTIDGLIGDDA--ERL 58

		

		Query: 56  DAIILALPGVVKDGVAVWCPNIGSGWIDVPIAKMLHDILPDVPLLIENDA-----KLAGL 110

		                     V   +      +   W +VP   +   +   +PLL++N       ++   

		Sbjct: 59  TGASGL--STVPSVLHEVRVMLEQYWPNVPHVLIEPGVRTGIPLLVDNPKEVGADRIVNC 116

		

		Query: 111 AETRSLHPIPTCSLYVTISTGI------GTGVITNGRIDPGLRNS 149

		                 +      + V   + I        G    G I PG++ S

		Sbjct: 117 LAAYHKYGTAA--IVVDFGSSICVDVVSAKGEFLGGAIAPGVQVS 159
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03605BINARYTOXINB320.001 Binary toxin B family signature.
		>BINARYTOXINB#Binary toxin B family signature.

		          Length = 764

		

		 Score = 31.6 bits (71), Expect = 0.001

		 Identities = 14/78 (17%), Positives = 34/78 (43%), Gaps = 5/78 (6%)

		

		Query: 43  NTNQTASS----DQSTSTGQASTNSDNQTNQTTGNTQKREEQKAQDQNAAAAAENKT-NS 97

		           + N+  S+     Q+ +  + ++ S   T++  GN +          + +A   N   ++

		Sbjct: 301 SKNEDQSTQNTDSQTRTISKNTSTSRTHTSEVHGNAEVHASFFDIGGSVSAGFSNSNSST 360

		

		Query: 98  PATDSSQTTSGTSSYSST 115

		            A D S + +G  +++ T

		Sbjct: 361 VAIDHSLSLAGERTWAET 378
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03615TCRTETOQM693e-14 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

		           signature.

		          Length = 639

		

		 Score = 68.7 bits (168), Expect = 3e-14

		 Identities = 36/135 (26%), Positives = 57/135 (42%), Gaps = 18/135 (13%)

		

		Query: 93  VAVMGHVDHGKTSLLDAILSTKTVAGEAG------------------GITQHISAYQTVR 134

		           + V+ HVD GKT+L +++L       E G                  GIT          

		Sbjct: 6   IGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW 65

		

		Query: 135 NNRPITLLDTPGHEAFAALRQHGATLTDVVVIVVAADDGVKPQTIEAIRFARTANAKIVV 194

		            N  + ++DTPGH  F A      ++ D  +++++A DGV+ QT       R      + 

		Sbjct: 66  ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIF 125

		

		Query: 195 AINKIDKEAANVDMV 209

		            INKID+   ++  V

		Sbjct: 126 FINKIDQNGIDLSTV 140
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03640ANTHRAXTOXNA320.023 Anthrax toxin LF subunit signature.
		>ANTHRAXTOXNA#Anthrax toxin LF subunit signature.

		          Length = 800

		

		 Score = 32.0 bits (72), Expect = 0.023

		 Identities = 22/96 (22%), Positives = 39/96 (40%), Gaps = 11/96 (11%)

		

		Query: 447 ENKRVKEERTQHTREVTQGRDFVNGKRRAEMQETMYETVSAQQTTDELNRLMEGGLDTPE 506

		             +  K E+ +  +E  + +D +N   + E   T       QQT D L ++       P+

		Sbjct: 43  IKRNHKTEKNKTEKE--KFKDSINNLVKTEF--TNETLDKIQQTQDLLKKI-------PK 91

		

		Query: 507 KVRAMYEALAGVQARVDISDAKGSELFSYSQGQRSV 542

		            V  +Y  L G     DI   +  EL   S+ +++ 

		Sbjct: 92  DVLEIYSELGGEIYFTDIDLVEHKELQDLSEEEKNS 127
21L336_RS03955L336_RS03975N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS03955-1131.154554CHAP domain-containing protein
L336_RS03960-1131.279051S41 family peptidase
L336_RS03965-1131.060063response regulator
L336_RS039700130.795106hypothetical protein
L336_RS03975-1140.51281850S ribosomal protein L27
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03955GPOSANCHOR432e-06 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

		          Length = 539

		

		 Score = 42.7 bits (100), Expect = 2e-06

		 Identities = 29/178 (16%), Positives = 65/178 (36%), Gaps = 10/178 (5%)

		

		Query: 51  QMQAINNQIRQYQSRADELGKQAQTLQVELDRLTNEKNAIQAQINLSQAQYDKLQRQITD 110

		           +++ +  +    ++R  EL K  +         + +   ++A+     A+   L++ +  

		Sbjct: 177 KIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEG 236

		

		Query: 111 TEKKIADNKDALGETIANMY-VDNSITPLEMLASSKSIGDYVDQQEYRASIRDTLNTTIE 169

		                  +   +    A    ++     LE     K++   ++     ++   TL     

		Sbjct: 237 AMNFSTADSAKIKTLEAEKAALEARQAELE-----KALEGAMNFSTADSAKIKTLEAEKA 291

		

		Query: 170 EIKALKIKLEKDKADVQVVLDRQKAQ-QASLAAK---EAQQAQLVQQTRNDEAAFQGL 223

		            ++A K  LE     +       +    AS  AK   EA+  +L +Q +  EA+ Q L

		Sbjct: 292 ALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSL 349
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03960HTHFIS290.031 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

		          Length = 484

		

		 Score = 29.4 bits (66), Expect = 0.031

		 Identities = 13/83 (15%), Positives = 31/83 (37%), Gaps = 7/83 (8%)

		

		Query: 207 ITRAKV----SDPSVRTSVKDGVGIMKISRFDDQTGTLASEAARSFKDQNVKAIILDLRD 262

		           +T A +     D ++RT +      +  + +D +  + A+   R     +   ++ D+  

		Sbjct: 1   MTGATILVADDDAAIRTVLNQ---ALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVM 57

		

		Query: 263 DGGGYLDAARQVASLWLDKKVIV 285

		                 D   ++     D  V+V

		Sbjct: 58  PDENAFDLLPRIKKARPDLPVLV 80
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03965HTHFIS755e-19 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

		          Length = 484

		

		 Score = 75.3 bits (185), Expect = 5e-19

		 Identities = 26/127 (20%), Positives = 55/127 (43%), Gaps = 3/127 (2%)

		

		Query: 7   KILLVEDDTALAAVYRSRLELEGFEINEVNNGEDALSAAMSYKPDLILLDAMMPKISGFD 66

		            IL+ +DD A+  V    L   G+++   +N         +   DL++ D +MP  + FD

		Sbjct: 5   TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

		

		Query: 67  VLDILRNTPETTNIRVIMLTALSQPKDKERAEQLGVDDYLVKSQVVIGDVVARVKHHLGM 126

		           +L  ++      ++ V++++A +      +A + G  DYL K    + +++  +   L  

		Sbjct: 65  LLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKP-FDLTELIGIIGRALAE 121

		

		Query: 127 DPGPQQA 133

		                  

		Sbjct: 122 PKRRPSK 128
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS03975RTXTOXIND270.007 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

		           signature.

		          Length = 478

		

		 Score = 27.5 bits (61), Expect = 0.007

		 Identities = 10/39 (25%), Positives = 17/39 (43%), Gaps = 5/39 (12%)

		

		Query: 4   VKAGGSSKNVHNNAGARLG---VKRFGGQVVTAGQVLVR 39

		           +   G SK +     + +    VK   G+ V  G VL++

		Sbjct: 90  LTHSGRSKEIKPIENSIVKEIIVKE--GESVRKGDVLLK 126
22L336_RS04960L336_RS04990N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
L336_RS04960-2160.010317transcription termination/antitermination
L336_RS05780-2140.232965preprotein translocase subunit SecE
L336_RS04970-1120.477603hypothetical protein
L336_RS04975-111-0.294315PAS domain-containing protein
L336_RS05785010-0.233157M48 family metallopeptidase
L336_RS04985112-0.187064AAA family ATPase
L336_RS04990012-0.196038DUF11 domain-containing protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS04960FLGMRINGFLIF280.021 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

		          Length = 559

		

		 Score = 28.0 bits (62), Expect = 0.021

		 Identities = 11/57 (19%), Positives = 25/57 (43%), Gaps = 11/57 (19%)

		

		Query: 100 GTEPTPVSDAEISKI----KKRMGVEDPKHQIDFQPGEVVNITDGPFKGFDGAVSEI 152

		             +P P++  ++ +I    ++ MG          + G+ +N+ + PF   D    E+

		Sbjct: 397 DGKPLPLTADQMKQIEDLTREAMG-------FSDKRGDTLNVVNSPFSAVDNTGGEL 446
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS05780TYPE3IMSPROT290.003 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

		           family signature.

		          Length = 354

		

		 Score = 29.0 bits (65), Expect = 0.003

		 Identities = 15/61 (24%), Positives = 23/61 (37%), Gaps = 4/61 (6%)

		

		Query: 53  FFAIWRYLKGSWYELRQVRWPDRRTTWAMTGALL----LFTAFFVVVILLLDAGFQYLFN 108

		              IW  +KG+   L Q+          + G +L    +      VVI + D  F+Y   

		Sbjct: 151 SILIWIIIKGNLVTLLQLPTCGIECITPLLGQILRQLMVICTVGFVVISIADYAFEYYQY 210

		

		Query: 109 I 109

		           I

		Sbjct: 211 I 211
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS04975PF06580416e-06 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

		          Length = 349

		

		 Score = 41.4 bits (97), Expect = 6e-06

		 Identities = 33/154 (21%), Positives = 66/154 (42%), Gaps = 29/154 (18%)

		

		Query: 333 TLSNVQLMMTREDIPKATLATNVTIAHDQVLFLAKMVNDLSTLSRAERGVADAPESINVS 392

		            L+N++  +  ED  KA                 +M+  LS L R     ++A + ++++

		Sbjct: 178 ALNNIR-ALILEDPTKA----------------REMLTSLSELMRYSLRYSNARQ-VSLA 219

		

		Query: 393 DLIH--DLYNEYAPQAETKGLKFNLDLDPGLGEVVASRLYLKELLQNFVTNAIKY----- 445

		           D +   D Y + A       L+F   ++P + +V    +    L+Q  V N IK+     

		Sbjct: 220 DELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQVPPM----LVQTLVENGIKHGIAQL 275

		

		Query: 446 TKEGSVLVSVKAADTTLKFSVQDTGIGMSKSDQK 479

		            + G +L+     + T+   V++TG    K+ ++

		Sbjct: 276 PQGGKILLKGTKDNGTVTLEVENTGSLALKNTKE 309
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
L336_RS04990CHLAMIDIAOM6502e-08 Chlamydia cysteine-rich outer membrane protein 6 si...
		>CHLAMIDIAOM6#Chlamydia cysteine-rich outer membrane protein 6

		           signature.

		          Length = 547

		

		 Score = 49.7 bits (118), Expect = 2e-08

		 Identities = 31/97 (31%), Positives = 48/97 (49%), Gaps = 7/97 (7%)

		

		Query: 291 CVEDKPGVSITKMVNNAEHQTVLVGSIY-------EYEISVKNTGNVPLKDVVVTDTAPA 343

		           C   K   S+T ++N    Q  + G+ +       EY ISV N G++ L+DVVV DT   

		Sbjct: 299 CGGHKNTASVTTVINEPCVQVSIAGADWSYVCKPVEYVISVSNPGDLVLRDVVVEDTLSP 358

		

		Query: 344 GVAFISASSGEITGGTWRNTLPTLAVGESLSFTIAAK 380

		           GV  + A+  +I+      T+  L  GESL + +  +

		Sbjct: 359 GVTVLEAAGAQISCNKVVWTVKELNPGESLQYKVLVR 395

		

		

		

		 Score = 29.3 bits (65), Expect = 0.040

		 Identities = 34/111 (30%), Positives = 47/111 (42%), Gaps = 8/111 (7%)

		

		Query: 297 GVSITKMVNNAEHQTVLVGSIYEYEISVKNTG-----NVPLKDVVVTDTAPAGVAFISAS 351

		           GV+ T M        V VG    Y I V N G     NV L      +  P  V+F   +

		Sbjct: 429 GVAATHMCVVDTCDPVCVGENTVYRICVTNRGSAEDTNVSLMLKFSKELQP--VSFSGPT 486

		

		Query: 352 SGEITGGTWR-NTLPTLAVGESLSFTIAAKIAAYKAGVIKNIACVETPTVP 401

		            G ITG T   ++LP L   E++ F++  K  +      + I   +T TVP

		Sbjct: 487 KGTITGNTVVFDSLPRLGSKETVEFSVTLKAVSAGDARGEAILSSDTLTVP 537

 
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