Figures

1 tctagaattttcaaatagcagtttatcattttccgtaaatatgaa 46 aaacattcttaaattgtttttttaatagttacttttccaaaaaat 91 acaaataaaactgttactgaaactttcaaaaataccaaatttgga 136 ATGTATTCTCGAGTGCAG 3' primer1 1 M Y S R V Q T A E E I P 136 ttccctcatATGTATTCTCGAGTGCAGACAGCAGAAGAGATTCCC 13 H E G N P A I K D Q D Y V K A 181 CACGAGGGAAATCCTGCTATCAAGGACCAGGACTATGTGAAAGCC 28 D D L 226 GATGACTTgtacgtttttaacattttaaatttctttatttctcta 31 Y T E A L Q L T T D E D 271 aattttcagGTACACAGAAGCTCTTCAGTTGACCACCGATGAGGA 316 CGTTCTCTACCGAAATC 3' primer2 43 K S L R P V L Y R N R A L A R 316 TAAATCACTGCGGCCCGTTCTCTACCGAAATCGTGCATTGGCACG 58 L R R D D F E P A Q S D C N K 361 ACTGAGAAGAGATGATTTCGAACCAGCACAATCCGATTGTAACAA 73 A L E F D P A D V K A L F R R 406 AGCGTTAGAATTCGATCCTGCCGACGTTAAGGCTCTTTTCCGACG 88 A L A R E Q L G N V G P A F N 451 AGCTCTGGCTCGTGAACAGCTTGGTAATGTGGGTCCCGCGTTCAA 103 D A K E A L R L S P Q D K 496 TGATGCGAAAGAGGCATTGAGACTGTCTCCGCAAGACAAgtgagt 116 G I 541 gttcaaaaaatggaaagttattgtaaaaaggttttcagAGGCATC 118 V E T L Q P L V K A N N D K I 586 GTGGAAACCCTTCAACCCCTTGTAAAAGCTAACAATGACAAGATT 133 K Q T T S L A N K V T D M E K 631 AAGCAGACAACATCGTTGGCAAATAAGGTCACAGATATGGAGAAA 148 L A F R G E A K D V A Q K Q T 676 TTGGCATTCCGTGGAGAAGCAAAAGACGTAGCTCAGAAGCAGACG 163 A L N N L L V L C R E S E S G 721 GCATTGAACAACTTACTGGTGCTTTGCAGGGAGTCGGAGTCCGGA 178 A T S V W N Q 766 GCTACTTCAGTTTGGAATCAGgtaatcgtgaatcagaattgaaaa 185 G A I V P F I L N 811 tattattatcagttgacagGGTGCCATCGTTCCATTCATCTTGAA 194 L I N D K A E N E E L I V T G 856 CCTGATAAATGACAAGGCTGAGAACGAGGAACTCATTGTTACTGG 209 I R I L D E T L K N N A R A M 901 CATCCGAATCCTTGACGAGACCCTTAAGAACAATGCTCGTGCTAT 224 K F L A M H D S E G G K S A R 946 GAAATTCCTCGCGATGCACGATTCGGAAGGCGGGAAGTCTGCACG 239 F V C R L M C K R N N K E Y V 991 ATTTGTGTGCCGTCTGATGTGCAAAAGAAACAACAAGGAATACGT 254 D S A G T P V Q R V F N A M A 1036 TGATTCTGCTGGAACCCCGGTGCAGAGAGTATTTAATGCAATGGC 269 K M D R Q K E M K P D P E V A 1081 TAAAATGGACAGACAAAAAGAAATGAAACCGGATCCTGAAGTAGC 1126 CTACGGTTATTCGATA 5' primer7 284 D A N K L W I I R V L L E L Q 1126 AGATGCCAATAAGCTATGGATTATTCGTGTTCTTCTAGAACTTCA 299 E M L Q D P K V G V F Q R E T 1171 AGAGATGCTTCAAGACCCGAAAGTTGGAGTTTTTCAAAGAGAGAC 314 V I D L F L K N L M H M D G G 1216 GGTTATCGATCTTTTTCTCAAAAACCTGATGCATATGGATGGTGG 329 I P R G W S W K F V D E R G L 1261 AATTCCACGTGGATGGTCTTGGAAGTTTGTGGACGAGCGCGGATT 344 L V L L D V A S Q I P E Q C D 1306 GTTGGTTTTGTTAGACGTGGCGAGTCAGATTCCTGAACAGTGTGA 359 Y P V S A E T R Q H V A I C L 1351 TTATCCGGTGTCAGCTGAAACTAGACAGCACGTGGCGATTTGTCT 374 Q R L E E D M V F D T K R L I 1396 GCAAAGATTGGAAGAGGATATGGTTTTCGATACGAAGAGACTCAT 1441 AAGTTTCTCTCTCAGCTAAA 5' primer6 389 F K E R V D F F F N 1441 TTTCAAAGAGAGAGTCGATTTCTTTTTCAAgtaagttggcttagc 399 A 1486 tgttgtgaggtgtcagattttgaacaaacaatcattttcagTGCT 1531 ATCTCTCGCTGTACCGATGA 3' primer3 400 L I S R C T D D E E G H K Y R 1531 CTGATCTCTCGCTGTACCGATGATGAAGAAGGTCACAAGTACCGA 415 I K L S C F L I T M L Q G P V 1576 ATCAAGCTGTCCTGCTTCCTGATTACCATGCTTCAAGGCCCTGTC 430 D I G I N L I I N D Q L T P I 1621 GACATTGGAATCAATTTAATTATTAACGATCAATTGACTCCTATC 445 M L Q M A A S N N H L M Q G I 1666 ATGCTCCAAATGGCCGCTTCCAATAACCATTTGATGCAAGGAATT 460 A A E L I V G T I A K H E R A 1711 GCTGCTGAGTTGATTGTGGGCACCATAGCAAAGCACGAAAGAGCG 475 I N M L K V R I P V L R A L Y 1756 ATCAACATGCTGAAAGTACGAATTCCCGTTCTCCGTGCTCTTTAC 490 D S G D P T V K V R A L V G L 1801 GATTCAGGAGACCCGACAGTGAAAGTGAGAGCGTTAGTGGGTCTC 505 C K I G A A G G D D I S K A T 1846 TGTAAAATTGGTGCCGCTGGAGGAGATGATATTTCGAAGGCCACA 520 M K E E A V I S L A K T C K K 1891 ATGAAAGAAGAAGCTGTGATTAGTTTGGCAAAGACTTGTAAAAAG 535 F L L E T E K Y S V D I R R Y 1936 TTCCTTCTGGAAACAGAGAAATATTCCGTTGATATCAGAAGATAT 550 A C E G L S Y L S L D A D V K 1981 GCCTGTGAAGGACTCTCCTATCTGTCACTGGATGCAGATGTTAAA 565 E W I V D D S L L L R A L V I 2026 GAATGGATTGTAGATGATTCGTTGTTGCTCAGAGCTCTTGTGATT 580 L A K S A G P L C V Y T L A T 2071 CTCGCGAAAAGTGCTGGACCACTTTGTGTTTATACATTAGCCACA 595 I Y A N L S N A F E K P K V D 2116 ATCTATGCTAATCTTTCAAACGCATTTGAGAAACCAAAAGTCGAT 610 E E M V K L A Q F A K H H V P 2161 GAAGAAATGGTCAAACTTGCTCAATTCGCCAAGCATCATGTTCCA 625 E T H P K D T E D F V E K R V 2206 GAAACTCATCCCAAGGATACAGAAGATTTTGTGGAAAAACGAGTG 640 R A L V E E G A V P A C V A V 2251 CGGGCTCTGGTCGAAGAAGGGGCAGTACCAGCGTGTGTAGCTGTG 655 S K T E S K N A L E L I A R T 2296 AGCAAGACAGAGAGTAAAAATGCTCTTGAATTGATTGCCCGAACA 670 L L A F S E Y E D L R G R I I 2341 CTGCTGGCATTCTCCGAATACGAGGACCTACGTGGAAGAATCATT 2386 AAGAGGCT 685 A E G G T V L C L R L T K E A 2386 GCAGAAGGAGGAACTGTTCTTTGTTTAAGGCTGACCAAAGAGGCT 2431 TCTGGAGA 3' primer4 700 S G E G K I K A A H A I A K L 2431 TCTGGAGAAGGAAAAATCAAAGCAGCTCATGCGATTGCCAAGTTG 715 G S K A D P T I S F P G Q R A 2476 GGATCCAAAGCAGATCCGACTATTTCATTCCCAGGACAGCGAGCT 730 Y E V V K P L C D L L H P E V 2521 TATGAAGTTGTGAAGCCACTATGTGACCTTCTTCATCCTGAAGTT 745 E G K A N Y D A L L T L T N L 2566 GAAGGAAAAGCGAACTATGATGCTCTTCTGACACTGACCAATCTG 760 A S V S D S I R G R I L K E K 2611 GCAAGTGTTAGTGATTCGATTCGTGGTCGGATTCTGAAGGAGAAG 775 A I P K I E E F W F M T D H E 2656 GCGATTCCAAAGATTGAGGAGTTTTGGTTCATGACCGATCATGAG 790 H L R A A A S E L L L N L L F 2701 CATTTGAGAGCAGCAGCTTCAGAGCTTCTGTTGAATTTATTGTTC 805 F D K F Y D E T V A 2746 TTCGATAAATTCTATGATGAGACTGTAGCGgtgagtatacttcga 815 P G T D 2791 taaattctcaaactattataacatttctttcagCCTGGAACGGAT 819 R L K L W V L Y S A E V E D E 2836 CGTCTGAAGTTGTGGGTACTCTATTCTGCTGAAGTTGAAGACGAG 834 R L S R T S A A G F A I L T E 2881 CGTCTTTCTAGAACTTCCGCTGCTGGATTCGCTATCTTAACAGAA 849 A E N L C S R I L E E I Q S W 2926 GCCGAAAATCTCTGTAGCAGAATTCTTGAGGAAATTCAATCATGG 864 P E V F K D I A M H E D A E T 2971 CCAGAAGTTTTCAAGGATATCGCGATGCACGAAGACGCTGAAACA 879 Q R R G L M G I A N I M A S S 3016 CAACGTCGCGGACTGATGGGCATTGCAAATATTATGGCATCAAGT 3061 CAC 894 E K A C S E I V A S E V F R V 3061 GAGAAGGCGTGCTCAGAAATCGTTGCATCTGAAGTATTCCGAGTG 3106 GAGGAACGACACTGT 5' primer5 909 L L A V T K L G T I N Q E R A 3106 CTCCTTGCTGTGACAAAGCTCGGAACTATCAATCAAGAACGAGCG 924 G S T E Q A K R A I D A A E K 3151 GGATCTACAGAGCAAGCGAAACGGGCGATCGATGCCGCTGAAAAG 939 F G L V K P S D R E M Y E R D 3196 TTTGGACTTGTCAAACCGTCGGATCGAGAGATGTACGAGAGAGAC 954 T K M S T I Q E * 3241 ACCAAAATGAGCACTATTCAGGAATAAtgtt

A map showing primer annealing sites generated using Primer Show. The translation (shown in blue) was generated using the uppercase option, which treats the uppercase text as a continuous reading frame (use GenBank Feature Extractor to automatically convert exons to uppercase before using Primer Show). Each primer label (shown in red) is aligned with its anealing site on the direct strand or reverse strand on the DNA sequence below. The orientation of the primer is indicated by the 5' or 3' symbol placed after the primer sequence.

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