Manually set reading frame and coding nucleotides
This Dialog allows to translate coding nucleotide sequences into amino acid sequences.
Three nucleotides are called a triplet and are translated into one amino acid ().
The user specifies the and can select translated and untranslated sequence regions.
The coding nucleotides (Exons) are drawn bright white (), while
non-coding nucleotides are shown in gray (, gion UTRs).
The horizontal scroll-bar outlines the intron-exon structure of DNA.
Change coding/non-coding: By pressing the Insert-key or
Space-bar, nucleotides are turned into coding nucleotides. Those are
drawn bright white. Backspace and Delete, however, turns translation
int amino acids off and creates or prolongs introns and UTRs. Those
nucleotides are drawn gray.
Navigation:
The keys ←, →, Home, End, PgUp, PgDown and the mouse allow navigation within the DNA sequence.
The Shift-key in conjunction with these keys creates a block selection.
Alt+→ and Alt+← jumps to the next interesting sequence
position. These positions are intron/exon boundaries and selected
nucleotides or selected amino acids. Typing letter c jumps to
the nucleotide position corresponding to the alignment-cursor
position. Typing a number followed by letter e goes to the
n-th exon. Typing a number followed by letter 'i' or 'r' goes to the
i-th nucleotide position counted from left or right.
Num-prefix Some key-stroke can be applied n times. For
example typing a number followed by Insert will turn the next n
nucleotides into coding nucleotides.
Zoom:
Ctrl++ and Ctrl+- or Ctrl+Wheel
Saving/restoring cursor and scroll position: Type a
number followed by upper letter s to store the current position. For
reverting to a saved position type the number followed by
lower letters.
Mouse actions:
Left-click an amino acid moves the alignment cursor to the clicked residue.