#!/usr/bin/strap-protein-alignment # Number of characters per line set_characters_per_line 24 # Emphasize residues conserved in 70% of all rows set_conservation_threshold 70 # Color according to the chemical features of amino acids set_color_mode charge # The next two lines define the alignment aa_sequence MVLSAADKGNVKAAWGKVGGHAAEYGAEALERMFLSFPTTKTYFP, Canus aa_sequence -VLSAAERAQVKAAWGKI--QAGAHGAEALERMFLGFPTTKTYPF, Xenopus aa_sequence MILSAAERAQIKAAWGKVG-NAGAHGAEALD--FLGYPTTKSYPY, Drosophila # Xenopus starts with amino acid number 2 set_residue_index_offset 1, Xenopus # The next line sets the secondary structure. H=helix, E=extended sheet secondary_structure ---HHHHHHHHHHHHHHH--HHHHHHHHHHHHHHHHHHHHHHH-- , Canus # Accession ID provides a link to a database accession_id UNIPROT:P0A7B8 , Canus # With the command icon, the protein icon image is set icon http://www.bioinformatics.org/strap/toHTML/data/dog_temp.gif, Canus icon http://www.bioinformatics.org/strap/toHTML/data/Frog.gif, Xenopus icon fly_temp.gif, Drosophila balloon_text Ein Frosch, Xenopus balloon_text If you do not like this sequence
you can move it to the trash bin with the mouse., Canus # new_selection defines a new residue selection # add_annotation or set_annotation adds information to the residue selection new_selection Canus/activeSite, group_test, 11 set_annotation Canus/activeSite, Hyperrefs, http://en.wikipedia.org/wiki/Active_site set_annotation Canus/activeSite, Style, STYLE_BACKGROUND set_annotation Canus/activeSite, Color, #ff0000 add_annotation Canus/activeSite, Balloon, This residue is part of the active site new_selection Canus/a_selection, group_test, 2-18 set_annotation Canus/a_selection, Hyperrefs, http://en.wikipedia.org/wiki/N-terminus set_annotation Canus/a_selection, Style, STYLE_BACKGROUND set_annotation Canus/a_selection, Color, #00ffFF add_annotation Canus/a_selection, Balloon, Another balloon message add_annotation Canus/a_selection, Balloon, This can be dragged with the mouse to the trash. new_selection Xenopus/my_1st_selection, group_test, 30- set_annotation Xenopus/my_1st_selection, Hyperrefs, http://www.charite.de set_annotation Xenopus/my_1st_selection, Style, STYLE_UNDERLINE set_annotation Xenopus/my_1st_selection, Color, #00ff01 add_annotation Xenopus/my_1st_selection, Balloon, This is a selection. new_selection Xenopus/my_2nd_selection, group_test, 20-33 set_annotation Xenopus/my_2nd_selection, Hyperrefs, http://en.wikipedia.org/wiki/C-terminus set_annotation Xenopus/my_2nd_selection, Style, STYLE_UNDERLINE set_annotation Xenopus/my_2nd_selection, Color, #ff0000 add_annotation Xenopus/my_2nd_selection, Balloon, This is another selection. Drag it to the trash with the mouse. project_coordinates 1QPW_A , Xenopus